• Asian Pacific Journal of Cancer Prevention
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• v.15 no.20
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• pp.8685-8688
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• 2014

The objective of the present study was to investigate cloning, expression, and functions of the recombinant protein, Siva1. Siva1 gene was synthesized by RT-PCR from HCT116 cells. Plasmids were cleaved with the restriction endonuclease, BamH1/Sal1 and products were connected to pQE30, which underwent cleavage by BamH1/Sal1. The recombinant plasmid, pQE30-Siva1, was identified after digestion with restriction endonucleases followed by transformation into E. coli M15. Expression of Siva1 was induced by IPTG and identified by SDS-PAGE following purification with affinity chromatography. The results showed that size of Siva1 was 12 kDa, consistent with the molecular weight of the His-Siva1 fusion protein. Functional test demonstrated that Siva1 significantly inhibited the invasion and migration of HCT116 cells. It may thus find clinical application for control of cancers.

• Parasites, Hosts and Diseases
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• v.47 no.4
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• pp.413-415
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• 2009

Genotyping of Toxoplasma gondii has been performed in 23 PCR positive blood samples from stray cats in Korea. We used 2 separate PCR-restriction fragment length polymorphism (RFLP) patterns of SAG2 gene, amplifying the 5' and 3' ends of the locus. The results revealed that all samples belonged to the type I clonal lineage. Although T. gondii organisms were not isolated from the samples, the results of the present study represent that stray cats with T. gondii infection should be seriously concerned in our environment. Adequate and continuous control programs of stray cats are needed to reduce the risk of transmission of T. gondii as a zoonotic infection threatening the public health.

• Proceedings of the KIEE Conference
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• 1999.07g
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• pp.3027-3029
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• 1999

인간과 함께 협력하는 서어비스 산업에서 요구되는 로봇은 고정된 환경에서 단순 반복 작업을 하는 기존 산업용 로봇의 개념을 뛰어 넘어 변화하는 환경에서 이동하면서 물체 이송 및 조작 기능을 수행하는 작업 형태를 취한다. 일반적으로 서어비스 로봇은 이동 능력과 조작능력이 겸비된 mobile manipulator의 외양을 갖는 것이 가장 응용 분야가 넓다. 이러한 mobile manipulator는 구동 축만 10축 내외가 되므로 축별 서보 제어기와 종류별 센서 제어기들을 분산화 시키는 구조가 신뢰성, 연산 능력의 측면에서 바람직하다. 개방형 구조로써 서보 제어기와 센서 제어기의 결합에 따른 비용 절감을 위하여 저가의 고속 통신망을 사용하고, 제어기 구성 요소들의 수급 다양화와 보수 유지의 간편화를 위하여 개방화된 인터페이스 하드웨어를 채택한다. 또한 자체 동력원에 의해 구동되는 것이 작업범위에 제한을 가하지 않으므로 로봇 제어기가 로봇 내부에 실장되어 오랜 시간 운용 가능하도록 소형화된 구조를 지니도록 설계한다.

• Journal of Microbiology and Biotechnology
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• v.24 no.3
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• pp.363-370
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• 2014

Campylobacter jejuni is a prevalent foodborne pathogen worldwide. Human infection by C. jejuni primarily arises from contaminated poultry meats. Genes expressed in vivo may play an important role in the pathogenicity of C. jejuni. We applied an immunoscreening method, in vivo-induced antigen technology (IVIAT), to identify in vivo-induced genes during human infection by C. jejuni. An inducible expression library of genomic proteins was constructed from sequenced C. jejuni NCTC 11168 and was then screened using adsorbed, pooled human sera obtained from clinical patients. We successfully identified 24 unique genes expressed in vivo. These genes were implicated in metabolism, molecular biosynthesis, genetic information processing, transport, and other processes. We selected six genes with different functions to compare their expression levels in vivo and in vitro using real-time RT-PCR. The results showed that the selected six genes were significantly upregulated in vivo but not in vitro. In short, these identified in vivo-induced genes may contribute to human infection of C. jejuni, some of which may be meaningful vaccine candidate antigens or diagnosis serologic markers for campylobacteriosis. IVIAT may present a significant and efficient method for understanding the pathogenicity mechanism of Campylobacter and for finding targets for its prevention and control.

• Proceedings of the KIEE Conference
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• 1998.07f
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• pp.1937-1939
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• 1998

This paper presents a simple switching method to generate a PWM pattern mostly relevant to signle-phase three-level PWM rectifier. The adopted PWM switching pattern is performed in a manner similar to the space vector PWM method, which is popularly used in the three-phase rectifier and inverter. A set of possible voltages has been selected so that an equation with a time integral considered within a sampling period should be satisfied every sampling time. The simulation result shows that the proposed control scheme is good in some performance criteria such as unity power factor, low harmonic distortion of input current, dynamic response and voltage balancing of two series-connected DC capacitors.

• Proceedings of the KSME Conference
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• 2001.11a
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• pp.544-549
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• 2001

The effect of power supply voltage on the performance limits in a laboratory Magneto-rheological fluid based device was identified by experiments. It suggests that the frequency range of motion for control be limited by the voltage attenuation due to the coil inductance and the maximum power supply voltage set for practical use of MRF devices. In this work, the magnetic and electrical characteristics of MRF device are investigated and a design procedure is formulated to achieve the desired performance for a given power supply.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.31 no.9
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• pp.934-942
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• 2007

In this study, the characteristics of the load-sensitive hydraulic control system for closed center type of a wheel loader were analyzed using developed analysis program based on Amesim tool. From the parametric analysis, the effects of each factor were revealed. Through the simulation with varying parameters, the system parameter effects on the controllable region and the pump pressure and load pressure variations were studied. The results were compared with the experimental ones. The results and discussions of the present paper could aid in the design of a load-sensitive hydraulic control system for closed center type.

• Proceedings of the KIPE Conference
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• 1998.10a
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• pp.790-796
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• 1998

A novel mode of parallel operation of a modular 3-phase AC-DC flyback converter for power factor correction along with tight regulation was recently analyzed and presented. The advantage of the proposed converter does not require expensive high voltage and high current devices that are normally needed in popular boost type 3-phase converter. In this paper the detailed small signal analysis of the modular 3-phase AC-DC flyback converter is provided for control purposes and also experimental results are included to confirm the validity of the analysis.

• Proceedings of the KIPE Conference
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• 2010.07a
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• pp.314-315
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• 2010

This paper presents the design and development of Web control algorithm of continuous process control system using torque controlled vector inverter. Web algorithm used tension control with the tension sensor, and it is to calculate diameter of motor speed with maintain Web tension. The performance of Web control in this paper is verified by experiment.

• Proceedings of the KIEE Conference
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• 1998.07f
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• pp.1996-1998
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• 1998

This paper deals with the tension control of continuous processing line for steel plant. In order to improve the tension control performance, tension control is included in POR. FF compensation is applied to get the same speed characteristics during acceleration and deceleration period. In simulation roll diameter variation and inertia variation are considered. It becomes clear that the proposed tension control system has high accuracy performance.