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http://dx.doi.org/10.7314/APJCP.2014.15.20.8685

Preliminary Research on the Expression, Purification and Function of the Apoptotic Fusion Protein, Sival  

Zhang, Ya-Han (Suzhou Industrial Park Center for Disease Control and Prevention)
Yu, Lu-Gang (Suzhou Industrial Park Center for Disease Control and Prevention)
Zhu, Wan-Zhan (Suzhou Industrial Park Center for Disease Control and Prevention)
Wang, Sheng-Li (Suzhou Industrial Park Center for Disease Control and Prevention)
Wang, Dian-Dong (College of Life Science and Technology, Yangtze Normal University, Chongqing)
Yang, Yan-Xin (CSPC Zhongqi Pharmaceutical Technology) Co., LTD.)
Yu, Xia (Suzhou Center For Disease Prevention And Control)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.15, no.20, 2014 , pp. 8685-8688 More about this Journal
Abstract
The objective of the present study was to investigate cloning, expression, and functions of the recombinant protein, Siva1. Siva1 gene was synthesized by RT-PCR from HCT116 cells. Plasmids were cleaved with the restriction endonuclease, BamH1/Sal1 and products were connected to pQE30, which underwent cleavage by BamH1/Sal1. The recombinant plasmid, pQE30-Siva1, was identified after digestion with restriction endonucleases followed by transformation into E. coli M15. Expression of Siva1 was induced by IPTG and identified by SDS-PAGE following purification with affinity chromatography. The results showed that size of Siva1 was 12 kDa, consistent with the molecular weight of the His-Siva1 fusion protein. Functional test demonstrated that Siva1 significantly inhibited the invasion and migration of HCT116 cells. It may thus find clinical application for control of cancers.
Keywords
Siva1; cloning; expression; HCT116 cells; invasion; migration;
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