• Title/Summary/Keyword: center and periphery

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Rereading Tie Ning's Rose Gate - Focused on Female Characters' "Becoming" (철응(铁凝)의 『매괴문(玫瑰门)』 다시 읽기 - 여성인물의 '되기'를 중심으로 -)

  • Choi, Eun Jeong
    • Cross-Cultural Studies
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    • v.31
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    • pp.197-216
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    • 2013
  • This thesis analyzes various becomings of female characters in Rose Gate written by Tie Ning. By this analyzation, we can pick out what identity the female characters eager to have decomposed, what identity they hope to create, and what do these procedures mean. Si Qiwen's 'becoming male' presents strong desire to shift from periphery to center. However, it is meaningless when her tragic life can not be justified by those same female people. Zuxi's 'becoming wanderer' shows that Tie Ning refuses usual reality of woman's life which vest in man. Sumei's 'becoming mother' is the exact point where torn female ego gets to consolidate, and it means Tie Ning takes 'mother-becoming' as ultimate truth of woman's life. Besides, Tie Ning finds women's becoming something new as escaping from the other and she denies equation of man with savior.

Micromachined DNA Manipulation Device Using Circular Multi-Electrodes (원형 다중전극을 이용한 DNA 조작소자)

  • Moon, Sang-Jun;Yun, Jae-Young;Lee, Seung-S.;Nam, Hong-Kil;Chi, Yeun-Tae
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.27 no.7
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    • pp.1071-1075
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    • 2003
  • In this paper, we present a DNA manipulation device in the reaction chamber, which consists of a center electrode and circular outer electrodes of a reaction unit. The charged bio-molecules, DNA, are manipulated by the charge of the electrode in reaction unit. Controlling the induced dynamic electric field between the center electrode and the outer electrodes, concentration / repulsion / manipulation of bio-molecules are enabled at a periphery of electrode. Concentration of the fluorescent DNA at the center electrode is observed by applying +2V. Subsequently, applying -2V, the concentrated DNA is repelled rapidly from the center electrode, which makes dispersion completely in 0.5second. Furthermore, repeated applying +1V/-1V every 5 seconds at each outer electrode, we can circulate the DNA. We also investigate a micro-heater and sensor for DNA manipulation and reaction temperature. The coefficient of heat-resistance and heater temperature characteristic is 0.0043 and 100$^{\circ}C$/sec, respectively.

Culture of Human Umbilical Vein Endothlial Cells Using 96-well Microplates and Position Effects on Cell Growth

  • Lee, Soohyun;Insook Sohn;Park, Myungjin;Park, Inchul;Youngsook Sohn;Seokil Hong;Taeboo Choe
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.5 no.3
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    • pp.207-210
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    • 2000
  • When endothelial cells isolated isolated from human umbilical venis were cultred for 6dary using 96-well microplates, the final cell density in each was fiund not to be the same although the medium composition of each well was exactly the same. Cell growth in the wells located at the periphery of a microplate was low, while that in the central area of the plate was high. A possible cause for different rate of growth was proposed as the uneven concentration of oxygen in the culture medium of each well.

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Effects of fibrin-binding oligopeptide on osteopromotion in rabbit calvarial defects

  • Lee, Ju-A;Ku, Young;Rhyu, In-Chul;Chung, Chong-Pyoung;Park, Yoon-Jeong
    • Journal of Periodontal and Implant Science
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    • v.40 no.5
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    • pp.211-219
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    • 2010
  • Purpose: Fibronectin (FN) has been shown to stimulate bone regeneration in animal models. The aim of this study was to evaluate the capacity of bovine bone mineral coated with synthetic oligopeptides to enhance bone regeneration in rabbit calvarial defects. Methods: Oligopeptides including fibrin-binding sequences of FN repeats were synthesized on the basis of primary and tertiary human plasma FN structures. Peptide coated and uncoated bone minerals were implanted into 10 mm calvarial defects in New Zealand white rabbits, and the animals were sacrificed at 4 or 8 weeks after surgery. After specimens were prepared, histologic examination and histomorphometric analysis were performed. Results: At 4 weeks after surgery, the uncoated groups showed a limited amount of osteoid formation at the periphery of the defect and the oligopeptide coated groups showed more osteoid formation and new bone formation in the center of the defect as well as at the periphery. At 8 weeks, both sites showed increased new bone formation. However, the difference between the two sites had reduced. Conclusions: Fibrin-binding synthetic oligopeptide derived from FN on deproteinized bovine bone enhanced new bone formation in rabbit calvarial defects at the early healing stage. This result suggests that these oligopeptides can be beneficial in reconstructing oral and maxillofacial deformities or in regenerating osseous bone defects.

Gut Microbiota-Derived Short-Chain Fatty Acids, T Cells, and Inflammation

  • Kim, Chang H.;Park, Jeongho;Kim, Myunghoo
    • IMMUNE NETWORK
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    • v.14 no.6
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    • pp.277-288
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    • 2014
  • T cells are central players in the regulation of adaptive immunity and immune tolerance. In the periphery, T cell differentiation for maturation and effector function is regulated by a number of factors. Various factors such as antigens, co-stimulation signals, and cytokines regulate T cell differentiation into functionally specialized effector and regulatory T cells. Other factors such as nutrients, micronutrients, nuclear hormones and microbial products provide important environmental cues for T cell differentiation. A mounting body of evidence indicates that the microbial metabolites short-chain fatty acids (SCFAs) have profound effects on T cells and directly and indirectly regulate their differentiation. We review the current status of our understanding of SCFA functions in regulation of peripheral T cell activity and discuss their impact on tissue inflammation.

Green Spaces in the Urban Peripheries of Metropole Regions for Sustainable Development - Focused on Berlin, Milano and Seoul - (지속가능한 발전을 위한 대도시 외연부 녹지 활용 사례연구 - 베를린, 밀라노, 서울을 대상으로 -)

  • Hoh, Yun Kyeong;Chae, Jin-Hae
    • Journal of the Korean Institute of Landscape Architecture
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    • v.46 no.1
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    • pp.72-85
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    • 2018
  • This study focused on cases that led sustainable urban development through the construction and utilization of organic greenery systems linking green spaces of urban peripheries with metropolitan areas. To that end, Berlin - Brandenburg's regional parks in Germany and Milan's Raggi Verdi, a radial green axis project, in Italy were selected for analysis as case studies. As frameworks for this analysis, this study has established existing infrastructure accessibility and linkage, recycling and cooperative management. The results of the case study analysis are as follows: First, the specialized spatial strategy based on the individuality of the green space outside the city rather than a uniform landscape was used as the foundation of the sustainable development plan. Second, physical linkage from the center of the city to the periphery contributed to the sustainable development of the overall metropolis by improving the economic value of the surrounding area as well as ecological and environmental values. Third, the central management system was established to reduce the administrative inconvenience caused by multiple administrative districts in the green space of urban periphery. The implications of applying the above results to Seoul, Korea are as follows. First, it is necessary to establish a differentiation strategy by re-establishing the identity of a green landscape in the urban periphery, because the green spaces of Seoul's periphery are dispersed and mostly have a repetitious mountain landscape. Also, it is necessary to actively link peripheral mountains and urban green areas to create ecological value and economic value, and ultimately to help the sustainable development of the city. Finally, building an integrated management system is required to solve fragmented green space management departments in most of the urban periphery's green spaces. In conclusion, this study shows the significant possibility that the sustainable development of a metropolis can be derived from the utilization, linkage, and management of the green space in the urban peripheries, which is extraordinary compared to normal centralized urban development.

Optical Properties of Plastic Fiber Made from a Transparent Polymethylmethacrylate (투명한 PMMA 로 된 플라스틱 섬유의 광학적 성질)

  • Hee-Ju Chae;Yasuhiro Koike
    • Journal of the Korean Chemical Society
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    • v.33 no.4
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    • pp.431-435
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    • 1989
  • The refractive index, refractive-index distribution, birefringence, and the attenuation of the plastic fiber prepared from the transparent PMMA prerod were measured in accordance with continuous heat drawing temperature($T_d$). The refractive indices were decreased with $T_d$ elevation but the refractive-index distribution from the center of fiber to periphery was higher at lower $T_d$. The steep decrease of gradient index was only at the periphery of higher $T_d$. Birefringence was observed only below $220^{\circ}C$ and ranged $5{\times}10^{-4}$ to $6{\times}10^{-4}$. No birefringence was observed above $220^{\circ}C$. An elevation of $T_d$ brought about a monotonous decrease in the attenuation of the fiber.

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Sources of Residential Satisfaction of the Apartment Households in Seoul : A Contextual Analysis (서울 아파트 가구의 주거만족도의 원천에 관한 연구 -중심지와 외곽지 고충과 저층단지의 비교를 중심으로-)

  • 김용일;여홍구
    • Journal of the Korean Institute of Landscape Architecture
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    • v.16 no.3
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    • pp.47-58
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    • 1989
  • ^x Residential satisfaction by apartment housing type and by location was examined in Seoul Korea for a sample of 303 housewives disaggregated into four housing subgroups. These group differ in their personal characteristic by housing type and location. They showed significant differences in their levels of satisfaction and in their perception and evaluation of several community, neighborhood and housing unit attributes. A regression model of satisfaction for entire sample explain about 45% of the variation, but this conceals the compositional and the contextual differences between groups. Seperate regression for the four groups explain an average of 63% of the variation in residential satisfaction. Residents of high-rise and low-rise apartments both of center and periphery location differ significantly both from each other. Results show that certain dwelling, neighborhood and community context elicit dissatisfaction across the full sample. The objective contextual factor of housing type prove significant in most compositional subsamples, indicating that sources of residential satisfaction are not same in everywhere.

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Fracture Analysis of Hole Flanging Process for High Strength Steel Sheets (고강도 열연판재의 홀 플랜정시 파단특성연구)

  • 김정운;김봉준;문영훈
    • Transactions of Materials Processing
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    • v.10 no.6
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    • pp.465-470
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    • 2001
  • Hole flanging experiments are performed on flat circular plates with a hole in the center and the flangeability and fracture behaviors of TRIP steels and ferrite-Bainite duplex steels were examined. In the hole flanging, deformation by lip and petalling occurs when plates are struck by punches of various shapes and high circumferential strains induced in the target material cause radial cracking and the subsequent rotation of the affected plate material in a number of symmetric petals. In all cases, failure of the plate was due to lip fracture that results from multiple localized neckings that take place around the hole periphery where straining is most severe and a somewhat regular pattern was observed in a fracture shape. The neck characteristics in flange formation and the transition from the lip to petal mode at which fracture occurs were compared with two materials.

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Treatment of E. coli B with two Antibiotics and their Influence on $T_3$ phage Absorption (대장균(E. coli B)의 항생제처리가 $T_3$ phage의 부착에 미치는 영향)

  • Chung, Sang-Jin;Kim, Woon-Soo
    • Applied Microscopy
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    • v.10 no.1_2
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    • pp.19-25
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    • 1980
  • E. coli B was treated with colistin and kanamycin and the influence of these antibiotics on the absorption of $T_3$ phage was studied using the plaque counting method and the electron microscope. E. coli B treated with colistin was sharply inhibited on phage absorption and cell walls were severely damaged showing some spiny appearance around the walls. No influence of kanamycin was noted on phage absorption. Bacterial cells treated with kanamycin showed wave form in the structure of walls and a profound change was noted in the cytoplasm where it was concentrated along the periphery of the inner wall leaving the center of the cell to appear almost empty.

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