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http://dx.doi.org/10.3795/KSME-A.2003.27.7.1071

Micromachined DNA Manipulation Device Using Circular Multi-Electrodes  

Moon, Sang-Jun (포항공과대학교 대학원 기계산업공학부)
Yun, Jae-Young (포항공과대학교 대학원 생명과학부)
Lee, Seung-S. (포항공과대학교 대학원 생명과학부)
Nam, Hong-Kil (전남대학교 대학원 유전공학과)
Chi, Yeun-Tae (포항공과대학교 기계공학과)
Publication Information
Transactions of the Korean Society of Mechanical Engineers A / v.27, no.7, 2003 , pp. 1071-1075 More about this Journal
Abstract
In this paper, we present a DNA manipulation device in the reaction chamber, which consists of a center electrode and circular outer electrodes of a reaction unit. The charged bio-molecules, DNA, are manipulated by the charge of the electrode in reaction unit. Controlling the induced dynamic electric field between the center electrode and the outer electrodes, concentration / repulsion / manipulation of bio-molecules are enabled at a periphery of electrode. Concentration of the fluorescent DNA at the center electrode is observed by applying ＋2V. Subsequently, applying －2V, the concentrated DNA is repelled rapidly from the center electrode, which makes dispersion completely in 0.5second. Furthermore, repeated applying ＋1V/－1V every 5 seconds at each outer electrode, we can circulate the DNA. We also investigate a micro-heater and sensor for DNA manipulation and reaction temperature. The coefficient of heat-resistance and heater temperature characteristic is 0.0043 and 100$^{\circ}C$/sec, respectively.
Keywords
Circular Multi-Electrodes; DNA Manipulation; Fluorescence; Micro-Heater;
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