• Journal of Microbiology
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• v.34 no.1
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• pp.25-29
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• 1996

Since sequencing of randomly selected cDNA clones has been known to be a powerful approach to obtain information on gene expression pattern in specific cells or tissues, we have analyzed a 3'-directed cDNA library of vegetative mycelia of A. nidulans by single-pass sequencing of hundreds of randomly selected clones. Sequencing of 292 cDNA clones yielded 209 gene signatures (GSs) probably representing highly or lesser expressed genes in the vegetative mycelia. Among the 209 GSs, 25 (79 cDNA clones) appeared more than once and 184 only once. One GS appeared at a highest frequency of 6 times, 2 GSs5 times, 4 GSs 4 times, a GSs 3 times and 16 GSs twice. About 6.6% GSs comprizing of 13 GSs showed alternative polyadenylation. Among 23 redundant GSs, three were common in both mycelia and sexual organs, and 22 were probably mycelia-specific. Out of 209 GSs, 36 were identified in GenBank showing of 70% or greater similaritis. Only six GSs were for A. nidulans genes, and 13 GSs were of DNA or genes encoding cytoplasmic or organellar proteins. This pattern is similar to those in the human HepG2 cell line and in human colonic mucosa, although very few genes for nuclear proteins and for protein synthesis were in A. nidulans.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.37 no.9
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• pp.47-54
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• 2000

We designed asynchronous event logic library with 0.25um CMOS technology and interface chip for heterogeneous system with high-speed asynchronous FIFO operating at 1.6GHz. Optimized asynchronous standard cell layouts and Verilog models are designed for top-down design methodology. A Method for mitigating a design bottleneck when it comes to tolerate clock skew is described. This communication scheme using clock control circuits, which is used for the free of synchronization failures, is analyzed and implemented. With clock control circuit and FIFO, high-speed communication between synchronous modules operating at different clock frequencies or with asynchronous modules is performed. The core size of implemented high-speed 32bit-interface chip for heterogeneous system is about $1.1mm{\times}1.1mm$.

• Molecules and Cells
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• v.44 no.8
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• pp.602-612
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• 2021

DNA methylation is an important epigenetic mechanism affecting genome structure, gene regulation, and the silencing of transposable elements. Cell- and tissue-specific methylation patterns are critical for differentiation and development in eukaryotes. Dynamic spatiotemporal methylation data in these cells or tissues is, therefore, of great interest. However, the construction of bisulfite sequencing libraries can be challenging if the starting material is limited or the genome size is small, such as in Arabidopsis. Here, we describe detailed methods for the purification of Arabidopsis embryos at all stages, and the construction of comprehensive bisulfite libraries from small quantities of input. We constructed bisulfite libraries by releasing embryos from intact seeds, using a different approach for each developmental stage, and manually picking single-embryo with microcapillaries. From these libraries, reliable Arabidopsis methylome data were collected allowing, on average, 11-fold coverage of the genome using as few as five globular, heart, and torpedo embryos as raw input material without the need for DNA purification step. On the other hand, purified DNA from as few as eight bending torpedo embryos or a single mature embryo is sufficient for library construction when RNase A is treated before DNA extraction. This method can be broadly applied to cells from different tissues or cells from other model organisms. Methylome construction can be achieved using a minimal amount of input material using our method; thereby, it has the potential to increase our understanding of dynamic spatiotemporal methylation patterns in model organisms.

• The Journal of the Korea Contents Association
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• v.21 no.4
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• pp.825-832
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• 2021

In this study, by selecting specific aptamers that selectively detection on P. gingivalis, the main cause of periodontal disease, and purifying and identifying protein molecules that bind to the selected aptamers, the mechanism of action of P. gingivalis was investigated. A DNA library having 39 random sequences was prepared, and aptamers with specificity for P. gingivalis were selected using the SELEX method, and the nucleotide sequence was analyzed by cloning using PCR2.1 cloning vector. 8 of aptamers with different nucleotide sequences were selected, and modified weston blot was performed using APG-3 among the selected aptamers to identify 11 proteins that act directly, and proteins were analyzed. As a result, a protein that selectively binds to P. gingivalis was isolated and identified. Therefore, aptamer selectively binds and attaches to proteins related to inhibition of sugar metabolism and cell activity of P. gingivalis, suggesting the possibility of a sensor for diagnosis of periodontal disease.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.27 no.9C
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• pp.861-870
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• 2002

In this paper, with targeting on the drawback of RSA of operation speed, a new 1024-bit RSA cryptosystem has been proposed and implemented in hardware to increase the operational speed and perform the variable-length encryption. The proposed cryptosystem mainly consists of the modular exponentiation part and the modular multiplication part. For the modular exponentiation, the RL-binary method, which performs squaring and modular multiplying in parallel, was improved, and then applied. And 4-stage CSA structure and radix-4 booth algorithm were applied to enhance the variable-length operation and reduce the number of partial product in modular multiplication arithmetic. The proposed RSA cryptosystem which can calculate at most 1024 bits at a tittle was mapped into the integrated circuit using the Hynix Phantom Cell Library for Hynix 0.35㎛ 2-Poly 4-Metal CMOS process. Also, the result of software implementation, which had been programmed prior to the hardware research, has been used to verify the operation of the hardware system. The size of the result from the hardware implementation was about 190k gate count and the operational clock frequency was 150㎒. By considering a variable-length of modulus number, the baud rate of the proposed scheme is one and half times faster than the previous works. Therefore, the proposed high speed variable-length RSA cryptosystem should be able to be used in various information security system which requires high speed operation.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.48 no.1
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• pp.22-30
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• 2011

In this paper, we propose a memory efficient multi-rate Low Density Parity Check (LDPC) decoder for China Mobile Multimedia Broadcasting (CMMB). We find the best trade-off between the performance and the circuit area by designing a partially parallel decoder which is capable of passing multiple messages in parallel. By designing an efficient address generation unit (AGU) with an index matrix, we could reduce both the amount of memory requirement and the complexity of computation. The proposed regular LDPC decoder was designed in Verilog HDL and was synthesized by Synopsys' Design Compiler using Chartered $0.18{\mu}m$ CMOS cell library. The synthesized design has the gate size of 455K (in NAND2). For the two code rates supported by CMMB, the rate-1/2 decoder has a throughput of 14.32 Mbps, and the rate-3/4 decoder has a throughput of 26.97 Mbps. Compared with a conventional LDPC for CMMB, our proposed design requires only 0.39% of the memory.

• The Korean Journal of Mycology
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• v.23 no.2 s.73
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• pp.129-138
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• 1995

DNA fragment being able to restore in vitro activity of ${\beta}-1,3-glucan$ synthase was cloned by transformation of the Saccharomyces cerevisiae LP353 mutant strain with genomic library constructed in the YCp50. For the selection of transformants which showed no detectable phenotype linked to recovery of the defect in ${\beta}-1,3-glucan$ synthase activity, the colony autoradiography was succesfully applied. The restriction map of the cloned DNA fragment, which is 8.5-kb in length, was constructed. Both the YEplac195 and the YCp50 carrying the 8.5-kb fragment increased ${\beta}-1,3-glucan$ synthase activity of LP353 by two fold. Neither the YEplac195 nor the YCp50 carrying the 8.5-kb DNA fragment, however, complemented the temperature-dependent osmotic sensitivity which is another distinctive phenotype of LP353. Subcloning experiments indicated that a functional region was located in 4.8-kb BglII-KpnI fragment. The 4.8-kb fragment was also able to increase the level of ${\beta}-1,3-glucan$ content in cell wall as well as the resistance of cells to cell wall lytic enzyme, ${\beta}-1,3-glucanase$. The growth rate of the LP353 with 4.8-kb fragment was almost same as that of wild type strain in liquid medium with 1.2 M sorbitol at nonpermissive temperature. Taken these results together, the 4.8-kb fragment seemed to contain the BGS2 gene for ${\beta}-1,3-glucan$ synthase activity in yeast S. cerevisiae.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.30 no.5C
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• pp.344-354
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• 2005

In this paper, we proposed a new lifting architecture for JPEG2000 and implemented to ASIC. We proposed a new cell to execute unit calculation of lifting using the property of lifting which is the repetitious arithmetic with same structure, and then recomposed the whole lifting by expanding it. After the operational sequence of lifting arithmetic was analyzed in detail and the causality was imposed for implementation to hardware, the unit cell was optimized. A new lifting kernel was organized by expanding simply the unit cell, and a lifting processor was implemented for Motion JPEG2000 using it. The implemented lifting kernel can accommodate the tile size of $1024{\times}1024$, and support both lossy compression using the (9,7) filter and lossless compression using (5,3) filter. Also, it has the same output rate as input rate, and can continuously output the wavelet coefficients of 4 types(LL, LH, HL, HH) at the same time. The implemented lifting processor completed a course of ASIC using $0.35{\mu}m$ CMOS library of SAMSUNG. It occupied about 90,000 gates, and stably operated in about 150MHz though difference from the used macro cell for the multiplier. Finally, the improved operated in about 150MHz though difference from the used macro cell for the multiplier. Finally, the performance can be identified in comparison with the previous researches and commercial IPs.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.30 no.7C
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• pp.647-657
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• 2005

In this paper, we proposed a new lifting architecture for JPEG2000 and implemented to ASIC. We proposed a new cell to execute unit calculation of lifting using the property of lifting which is the repetitious arithmetic with same structure, and then recomposed the whole lifting by expanding it. After the operational sequence of lifting arithmetic was analyzed in detail and the causality was imposed for implementation to hardware, the unit cell was optimized. A new lifting kernel was organized by expanding simply the unit cell, and a lifting processor was implemented for Motion JPEG2000 using it. The implemented lifting kernel can accommodate the tile size of 1024$\times$1024, and support both lossy compression using the (9,7) filter and lossless compression using (5,3) filter. Also, it has the same output rate as input rate, and can continuously output the wavelet coefficients of 4 types(LL, LH, HL, HH) at the same time. The implemented lifting processor completed a course of ASIC using 0.35$\mu$m CMOS library of SAMSUNG. It occupied about 90,000 gates, and stably operated in about 150MHz though difference from the used macro cell for the multiplier. Finally, the improved operated in about 150MHz though difference from the used macro cell for the multiplier. Finally, the performance can be identified in comparison with the previous researches and commercial IPs.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.47 no.2
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• pp.107-121
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• 2021

Skin hypopigmentation, which is observed in albinism or vitiligo, occurs when melanin synthesis is decreased by genetic, epigenetic, and other factors. To identify drug candidates that can promote melanin synthesis in cells, we screened an epigenetic modulator library consisting of 141 cell-permeable, small molecule drugs. B16/F10 murine melanoma cells were treated with each drug at 0.1 𝜇M and melanin synthesis and cell viability were subsequently monitored. As a result, (-)-neplanocin A, 3-deazaneplanocin A (DZNep), and DZNep hydrochloride were found to increase cellular melanin synthesis without causing cytotoxicity. Because these three structurally related drugs exhibited similar dose-dependent effects on melanin synthesis and cell viability, DZNep was selected as a representative drug for additional experiments. DZNep increased intracellular melanin content and tyrosinase (TYR) activity. DZNep also induced the expression of TYR, tyrosinase-related protein 1 (TYRP1), and dopachrome tautomerase (DCT) at the mRNA and protein levels. DZNep also induced the mRNA and protein expression of microphthalmia-associated transcription factor (MITF), a key regulator of melanin synthesis. DZNep is a specific inhibitor of S-adenosylhomocysteine hydrolase and it caused the accumulation of S-adenosylhomocysteine that inhibits histone methyltransferases in cells. This study suggests that melanogenesis can be modulated by targeting S-adenosylhomocysteine hydrolase in certain cellular contexts.