Browse > Article
http://dx.doi.org/10.5392/JKCA.2021.21.04.825

A study on the Aptamer Specific Detection on P. gingivalis  

Shin, Ae-Ri (광주보건대학교 치위생과)
Publication Information
The Journal of the Korea Contents Association / v.21, no.4, 2021 , pp. 825-832 More about this Journal
Abstract
In this study, by selecting specific aptamers that selectively detection on P. gingivalis, the main cause of periodontal disease, and purifying and identifying protein molecules that bind to the selected aptamers, the mechanism of action of P. gingivalis was investigated. A DNA library having 39 random sequences was prepared, and aptamers with specificity for P. gingivalis were selected using the SELEX method, and the nucleotide sequence was analyzed by cloning using PCR2.1 cloning vector. 8 of aptamers with different nucleotide sequences were selected, and modified weston blot was performed using APG-3 among the selected aptamers to identify 11 proteins that act directly, and proteins were analyzed. As a result, a protein that selectively binds to P. gingivalis was isolated and identified. Therefore, aptamer selectively binds and attaches to proteins related to inhibition of sugar metabolism and cell activity of P. gingivalis, suggesting the possibility of a sensor for diagnosis of periodontal disease.
Keywords
P. gingivalis; Aptamer; SELEX;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Y. Tang, W. Wu, X. Zhang, Z. Lu, J. Chen, and W. Fang, "Catabolite control protein A of Streptococcus suis type 2 contributes to sugar metabolism and virulence," J Microbiol, Vol.50, No.6, pp.994-1002, 2012.   DOI
2 C. Tuerk and L. Gold, "Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase," Science, Vol.249, No.4968, pp.505-510, 1990.   DOI
3 A. D. Keefe, S. Pai, and A. Ellington, "Aptamers as therapeutics," Nat Rev Drug Discov, Vol.9, No.7, pp.537-550, 2010.   DOI
4 K. Germer, M. Leonard, and X. Zhang, "RNA aptamers and their therapeutic and diagnostic applications," Int J Biochem Mol Biol, Vol.4, No.1, pp.27-40, 2013.
5 C. Slocum, C. Kramer, and C. A. Genco, "Immune dysregulation mediated by the oral microbiome: potential link to chronic inflammation and atherosclerosis," J intern med, Vol.280, No.1, pp.114-128, 2016.   DOI
6 T. R. Mikuls, J. B. Payne, F. Yu, G. M. Thiele, R. J. Reynolds, G. W. Cannon, J. Markt, D. McGowan, G. S. Kerr, R. S. Redman, A. Reimold, G. Griffiths, M. Beatty, S. M. Gonzalez, D. A. Bergman, B. C. Hamilton, A. R. Erickson, J. Sokolove, W. H. Robinson, C. Walker, F. Chandad, and J. R. O'Dell, "Periodontitis and Porphyromonas gingivalis in patients with rheumatoid arthritis," Arthritis Rheum, Vol.66, No.5, pp.1090-1100, 2014.   DOI
7 H. Loe, CR. Schiott, G. Karring, and T. Karring, "Two years oral use of chlorhexidine in man.I. General design and clinical effects," J Periodontal Res, Vol.11, No.3, pp.135-144, 1976.   DOI
8 D. Adams and M. Addy, "Mouthrinses," Adv Dent Res, Vol.8, No.2, pp.291-301, 1994.   DOI
9 S. Shin, IH. Kim, W. Kang, J. K. Yang, and S. S. Hah, "An alternative to Western blot analysis using RNA aptamer functionalized quantum dot,s, Bioorg Med Chem Lett, Vol.20, No.11, pp.3322-3325, 2010.   DOI
10 김병희, 김성천, 장병탁, "기계학습에 의한 압타머칩 데이터 기반 심혈관 질환 단계의 예측," 한국정보과학회 한국컴퓨터종합학술대회 논문집, 제33권, 제1호, pp.85-87, 2006.
11 R. Stanislas and P. Jean, "VEGF as a target," Targeted Oncology, Vol.1, No.4, pp.239-240, 2006.   DOI
12 J. Folkman, "Angiogenesis," Annu Rev Med, Vol.57, pp.1-18, 2006.   DOI
13 S. Clais, G. Boulet, M. Kerstens, T. Horemans, W. Teughels, M. Quirynen, E. Lanckacker, ID. Meester, A. M. Lambeir, P. Delputte, L. Maes, and P. Cos, "Importance of biofilm formation and dipeptidyl peptidase IV for the pathogenicity of clinical Porphyromonas gingivalis isolates," Pathogens and Disease, Vol.70, No.3, pp408-413, 2014.   DOI
14 R. L. Mandell, J. L. Ebersole, and S. S. Socransky, "Clinical immunologic and icrobiologic features of active disease sites in juvenile periodontitis," J Clin Periodontol, Vol.14, No.9, pp.534-540, 1987.   DOI
15 B. E. Siegrist, F. A. Gusberti, M. C. Sreex, H. P. Weber, and N. P. Long, "Efficacy of supervised rinsing with chlorhexidine digluconate in comparison to phenolic and plant alkaloid compounds," J Periodontal Res, Vol.21, No.16, pp.60-73, 1986.   DOI
16 C. Cugini, V. Klepac-Ceraj, E. Rackatyte, J. E. Riggs, and M. E. Darvey, "Porphyromonas gingivalis : keeping the pathos out of the biont," J oral Microbiol, Vol.5, pp.1-10, 2013. DOI: 10.3402/jom.v5i0.19804   DOI
17 NJ. Lopez, "Occurrence of Actinobacillus actinomycetemcomitans, Porphryomonas gingivalis and Prevotella intermedia in progressing adult periodontitis," J Periodontal, Vol.71, No.6, pp948-952, 2000.   DOI
18 H. H. Jo and CI. Ban, "Aptamer-nanoparticle complexes as powerful diagnostic and therapeutic tools," Exp Mol Med, Vol.48, No.5, pp.1-9, 2016.
19 박병주, 옥승호, "DNA aptamers를 이용한 Streptococcus mutans의 부착억제," 한국산학기술학회논문지, 제19권, 제3호, pp.382-388, 2018.   DOI
20 C. C. Kietzman and M. G. Caparon, "Distinct time-resolved roles for two catabolite-sensing pathways during Streptococcus pyogenes infection," Infect Immun, Vol.79, No.2, pp.812-821, 2011.   DOI
21 R. P. Darveau, A. Tanner, and R. C. Page, "The microbial challenge in periodontitis," Periodontol 2000, Vol.14, No.1, pp.12-32, 1997.   DOI
22 나용철, 김강주, 유형근, 신형식, "성인형 치주염 환자에서 분리한 Actinobacillus actinomycetemcomitans의 항생제 내성에 관한 연구," 圓光齒醫學, 제6권, 제1호, pp.23-40, 1996.
23 M. J. Chun, K. J. Park, and S. H. Ohk, "Putative Down-stream Signaling Molecule of GTPase in Porphyromonas gingivalis," Prikl Biokhim Mikrobiol, Vol.48, No.4, pp.389-393, 2012.
24 http://dongascience.donga.com/news/view/43897
25 J. Aduse-Opoku, J. M. Slaney, A. Hashim, A. Gallagher, R. P. Gallagher, M. Rangarajan, K. Boutaga, M. L. Laine, A. J. V. Winkelhoff, and M. A. Curtis, "Identification and characterization of the capsular polysaccharide (K-antigen) locus of Porphyromonas gingivalis," Infect Immun, Vol.74, No.1, pp.449-460, 2006.   DOI
26 P. E. Petersen and H. Ogawa, "Strengthening the prevention of periodontal disease : The WHO approach," J Periodontal, Vol.76, No.12, pp.2187-2193, 2005.   DOI
27 M. Taba, J. J. Kinney, A. S. Kim, and W. V. Giannobile, "Diagnostic biomarkers for oral and periodontal diseases," Dent Clin North Am, Vol.49, No.3, pp.551-571, 2005.   DOI
28 J. P. Park, H. J. Shin, S. G. Park, H. K. Oh, C. H. Choi, H. J. Park, M. S. Kook, and S. H. Ohk, "Screening and development of DNA aptamers specific to several oral pathogens," J Microbiol Biotechnol, Vol.25, No.3, pp393-398, 2015.   DOI