• Journal of Plant Biotechnology
• /
• v.30 no.3
• /
• pp.251-255
• /
• 2003

An efficient system for in vitro bulblet formation of Lilium oriental hybrids(cvs). Casa Blanca and Siberia is described. Transverse thin cell layer(tTCL)(1mm thick) explants of 'Casa Blanca' formed bulblets at a frequency of 97.7％ when cultured on Murashige and Skoog (MS) medium supplemented with 1mg/L 2,4 dichlorophenoxyacetic acid(2,4-D) (On average 15.6 bulblets were formed per explant). The frequency of bulblet formation was drastically reduced when the explant ghickness was thinner than 1 mm. Explants from the outermost layer of bulb scale produced greater frequency of bulblet formation than middle or innermost layer. Among auxins supplemented to culture medium at 1 mg/L, 2,4-D led to greater frequency of bulblet formation on explants than dicamba, picamba, or phenylacetic acid(PAA). tTCL explants from the middle region of the outermost layer bulb scale yielded greater frequency of bulblet formation than the upper or lower region. tTCL stem explants of 'Siberia' formed bulblets at a frequency of 95.3% when cultured on MS medium with 1 mg/L 2,4-D(On average 9.1 bulblets were formed per explant). The system estabilished in this study will be useful for in vitro rapid propagation and genetic transformation of Lilium Oriental hybrids.

• Proceedings of the Korean Geotechical Society Conference
• /
• 2008.03a
• /
• pp.1240-1249
• /
• 2008

Jacket anchor was developed to increase the pullout resistance of general ground anchor in soft ground, and the mechanism of pullout resistance of jacket anchor was analyzed. Also, the ultimate bond stress of jacket anchor was estimated by ultimate resistance which is determined by field tests. Grout milk was injected into the jacket to make grout bulb of jacket anchor. The formation of grout bulb of jacket anchor increases the diameter of grout bulb, ground strength and confining pressure between anchor grout and soil. From the twelve field test results, it was observed that the pullout resistance of jacket anchor is 15.38~295.02%(average 83.53%) greater than that of general ground anchor, and plastic deformation of jacket anchor is 20.78~1,496.45%(average 288.78%) smaller than that of general ground anchor at the same load cycle. Especially, it was investigated that the increase of ultimate resistance over 200% and the reduction of plastic deformation over 600% was obtained in gravel layer. It means that the jacket anchor is superior to the general ground anchor in gravel layer. Finally, the ultimate bond stress was proposed to design jacket anchor.

• Horticultural Science & Technology
• /
• v.35 no.3
• /
• pp.373-380
• /
• 2017

Amaryllis ($Hippeastrum{\times}johnsonii$) is commonly propagated by three methods: seeds, offset bulblets, and twin scaling. Since the number and size of bulblets produced by these methods are low, we conducted an experiment to evaluate different bulb cutting methods for propagation. For this purpose, bulbs (circumference of 30 - 35 cm) were sectioned or notched into 8, 12, or 16 segments or twin-scaled into 48, 72, or 96 segments for bulblet formation. Our results show that the largest number of bulblets was produced by the twin scaling method, but they take longer to grow to a final, commercial size. Sectioning and notching resulted in larger, but fewer bulblets than twin-scaling. Compared to notching, sectioning provided more space for the bulblets to grow, and is therefore the recommended method. While increasing the number of sections cut from a single bulb resulted in a larger number of bulblets, the diameter of the bulblets decreased. Therefore, sectioning the bulb into 8 segments was the best method for producing an acceptable number of vigorous bulblets.

• Korean Journal of Plant Tissue Culture
• /
• v.25 no.1
• /
• pp.27-31
• /
• 1998

Immature hybrid embryos of H. hybridum, 'Picottee', 'White Christmas', 'Eldorado', 'Origin', 'Red Lion', 'elstar', 'Crypsy' were cultured on the MS medium supplemented with various concentrations of 2,4-D, NAA, BA and TDZ. Among the treatments, NAA were more effective for the shoot regeneration and bulblet formation than other treatment. Addition of 0.5 ㎎/L NAA was effective for bulblet induction from explant Shoot regeneration was most effective on the medium with 1.0㎎/L NAA and 2.0 ㎎/L TDZ. The addition of 1.0-2.0㎎/L TDZ induced numerous shoots per explant but strongly inhibited root development when compared to 1.0-2.0㎎/L BA. When bulb scale segments of 'Star Van Holland' was incubated, bulblet formation was the most effective on MS medium with 0.5㎎/L NAA.

• Korean Journal of Plant Tissue Culture
• /
• v.22 no.2
• /
• pp.83-87
• /
• 1995

Regulation of organ differentiation by growth regulators was investigated through the shoot-tip and bulb-scale cultures of Lilium longiflorum (cv Georgia). When shoot tips were placed on MS medium supplemented with 0.1 mg/L NAA alone or 0.1 mg/L NAA and 0.1 mg/L BA, axillary shoots were proliferated. Root diffentiation and growth were stimulated on the basal medium. Although growth regulation did not seem to be necessary when bulb scales were used as explants for shoot differentiation, its differentiation was promoted vigorously by 0.2 mg/L NAA, but suppressed by BA. Bulblets were formed from bulb-scale-derived plantlets cultured on MS medium supplemented with 0.1 mg/L IBA. And more bulblets were formed from the plantlet in MS medium supplying 0.2 mg/L NAA with 6% than3% sucrose

• Journal of Microbiology and Biotechnology
• /
• v.33 no.5
• /
• pp.634-643
• /
• 2023

Chronic obstructive pulmonary disease (COPD), one of the leading causes of death worldwide, is caused by repeated exposure to harmful matter, such as cigarette smoke. Although Lilium longiflorum Thunb (LLT) has anti-inflammatory effects, there is no report on the fermented LLT bulb extract regulating lung inflammation in COPD. Thus, we investigated the protective effect of LLT bulb extract fermented with Lactobacillus acidophilus 803 in COPD mouse models induced by cigarette smoke extract (CSE) and porcine pancreas elastase (PPE). Oral administration of the fermented product (LS803) suppressed the production of inflammatory mediators and the infiltration of immune cells involving neutrophils and macrophages, resulting in protective effects against lung damage. In addition, LS803 inhibited CSE- and LPS-induced IL-6 and IL-8 production in airway epithelial H292 cells as well as suppressed PMA-induced formation of neutrophil extracellular traps in HL-60 cells. In particular, LS803 significantly repressed the elevated IL-6 and MIP-2 production after CSE and LPS stimulation by suppressing the activity of the nuclear factor kappa-light-chain-enhancer of activated B (NFκB) in mouse peritoneal macrophages. Therefore, our results suggest that the fermented product LS803 is effective in preventing and alleviating lung inflammation.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.51 no.5
• /
• pp.401-407
• /
• 2006

This study was conducted to investigate the effect of onion cultivation under paddy and upland condition on the yield and storage of onion bulbs. In comparison of soil conditions, nitrogen, phosphate, potassium content and average temperature in upland condition were higher than those of paddy condition, but calcium, magnesium, sodium and water content in paddy condition were higher than those of upland condition. The growth of aerial part was better in upland cultivated condition. It showed that bulb formation in paddy cultivated condition was faster during in early growth stage, but was slower during in late growth stage. Average bulb size and weight were higher in upland cultivated condition, but dry weight per 100 g fresh weight and bulb hardness were higher in paddy cultivated condition. Sugar contents, inorganic compounds, nitrogen, phosphate and potassium content of onion were higher in upland cultivated condition, but calcium, magnesium and sodium content of onion were higher in paddy cultivated condition. In distribution of onion bulb size, most of large sized onions were produced in upland cultivated condition. Average bulb production for seed harvesting were $63{\sim}70%$ higher in paddy cultivated condition than that of upland cultivated condition. The rotted rate of onion was about $27.2{\sim}34.6%$ in paddy, $37.5{\sim}51.4%$ in upland cultivated condition respectively, showed favorable result as the difference of $8.7{\sim}24.2%$ in paddy cultivated condition.

• Proceedings of the Korean Society of Plant Biotechnology Conference
• /
• 2003.10a
• /
• pp.129-129
• /
• 2003

• Korean Journal of Plant Resources
• /
• v.19 no.1
• /
• pp.88-92
• /
• 2006

Nerine was originated from South Africa and around 30 kinds of species are distributed in worldwide. In present study we have characterized the division methods and growing conditions for the bulb propagation of Nerine. Result indicated that new bulb formation, and growth of N. bowdenii cultivars was better after dormancy stage compared to post-harvest and dormancy stages, under twin-scale method at $20^{\circ}C$ Similarly, new bulb tissue density was higher at $20^{\circ}C$ compared to other temperatures. Moreover, N. bowdenii 'Favourite' exhibited better growth than N. bowdenii 'Stepanii' and vermiculite was found to be most suitable growing medium for Nerine bulb propagation.

• Proceedings of the Korean Geotechical Society Conference
• /
• 2006.03a
• /
• pp.424-433
• /
• 2006

This paper studies the field applicability of the Special Chemical grouting Method(SCM) in reinforcing and reducing permeability of the back of an existing continuous wall. SCM uses double rod which imposes intermediate pressure$(981\sim9,810kPa)$ to disturb, cut, discharge, and mix the ground. It is observed that a bulb is formed by using cement paste and environmentally friendly injection materials with minimal alkali leaching. Uniaxial compression tests, test for chemical properties and fish poison test are performed. Test results indicate that the method results in higher durability, less leaching through use of the environmentally friendly injection material, and faster mobilization of the strength. In addition, field tests confirm the formation of the bulb and the seepage cutoff wall.