• 한국육종학회지
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• 제40권2호
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• pp.113-118
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• 2008

This study was carried out to investigate the chemical composition of peanut mutants induced by gamma radiation (Co-60) at 300 Gy in seeds of the cultivar Shinnamkwang. The protein contents of twenty-eight peanut mutants ranged from 23.3% to 31.7% and were increased by from 0.5% to 8.4% in fifteen mutants lines from the 26.8% of the original variety, cv. Shinnamkwang. Lipid contents of in mutants ranged from 43.2% to 53.5%, an increase of 0.2% to 5.7% from the 47.8% of the original variety. The range of unsaturated oleic acid in 28 mutants was from 38.9% to 56.9% an increase of from 1.3% to 14.0% from the 50.6% in the original variety. Linoleic acid, the highest unsaturated fatty acid, constituted 32.6% meanly of mutants, it was 17.4% lesser than oleic acid, ranging from 25.9% to 42.0%. Palmitic acid (16:0) contents ranged from 8.6% to 11.1%, and the mutant line-9 had the highest content. The ratio of oleic to linoleic acid was 0.9 to 2.2. A negative coefficient (r=-0.98**) was obtained between oleic and linoleic acid, but for other fatty acids, no significant relation was observed. Similarly, a negative coefficient of r=-0.68**was observed between saturated and unsaturated acids. The sucrose compositions of mutants ranged from 2.6% to 6.2%.

• 한국육종학회지
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• 제51권3호
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• pp.214-221
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• 2019

'아람'은 보석(IT213209)과 Camp (IT267356)를 모본으로 2007년 인공교배 하여 F₁, F₂ 분리집단을 전개하고 F₃-F₄ 세대는 세계 채소 센터(AVRDC, Asian Vegetable Research and Development Center)에서 세대촉진을 수행하고 F₅ 세대에서 계통을 선발하였다. 2012년~2013년 생산력검정시험을 통해 수량과 농업적 형질을 평가하고, 2014~2016년 수원, 나주, 달성, 제주에서 지역적응시험을 수행하여 지역별 적응성과 재배 안정성을 평가하였다. 아람은 유한신육형이며 엽형이 삼각형, 화색이 백색, 모용색이 회색, 협색은 황색이며 종실은 소립 구형으로 백립중이 9.9 g으로 풍산나물콩보다 1.0 g 가볍고 종피색은 황색, 배꼽색은 담갈색의 질적 특성을 가지고 있다. 개화기는 8월 5일, 성숙기는 10월 15일로 만숙종이며 풍산나물콩 보다 7일 느리다. 경장은 65 cm, 착협고는 13 cm, 마디수는 16개, 분지수는 4.5개이며 분지가 길고 각도가 작은 도원추형 초형이다. 내병성 검정결과 불마름병, 종자 이병립율, 검은뿌리 썩음병, 콩나방 저항성 등이 모두 강하였으며, 콩모자이크바이러스는 G5, G6H, G7H 접종 시 모자이크 증상이 나타났으나 3년간의 자연 이병 검정결과에서는 병징이 조사되지 않았다. 발아특성은 발아세와 발아율 모두 풍산나물콩보다 우수하였으며 콩나물 특성은 풍산나물콩과 비슷한 수준이었다. 수량성은 지역적응시험 결과 남부지역 평균 3.59 ton/ha로 풍산나물콩 대비 12% 증수되었다.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2015년도 춘계학술대회 및 임시총회
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• pp.33-33
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• 2015

Clubroot disease is the major threat to the production of Chinese cabbage (Brassica rapa L.) in Japan. Although the breeding of the clubtoot resistant (CR) cultivars is one of the most efficient ways to control this disease, the CR cultivars do not always have effects due to the breakdown of resistance. Therefore, it is necessary to develop the breeding strategy to accumulate multiple CR genes in a single cultivar effectively. We have identified two incomplete dominant CR loci, Crr1 and Crr2, which are originated from the European CR turnip Siloga. To investigate the effectiveness of marker-assisted selection (MAS) for CR breeding, the inbred line with Crr1 and Crr2 was crossed with parental lines of the existing CR $F_1$ cultivar of Chinese cabbage, followed by 5 times of MAS and backcrossing. The $F_1$ derived from a cross between the resulting parental lines improved the clubroot resistance as expected and had the same morphological characters as the original $F_1$ cultivar. We have shown that the Crr1 locus comprised two loci: Crr1a, which by itself conferred resistance to the mild isolate; and Crr1b, which had a minor effect, but was not required for Crr1a-mediated resistance. Further genetic analysis suggested that Crr1b was necessary to acquire resistance to the more virulent isolate in combination with Crr2. Molecular characterization of Crr1a encoding TIR-NB-LRR class of R protein revealed that there were at least 4 alleles in Japanese CR cultivars of Chinese cabbage. PCR analysis with Crr1a-specific markers demonstrated that the functional alleles were predicted to be present in European CR turnips, Debra and 77b besides Siloga, whereas rarely in Japanese CR cultivars, indicating that Crr1a is an useful source to improve the resistance of Chinese cabbage cultivars.

• Current Research on Agriculture and Life Sciences
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• 제28권
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• pp.39-46
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• 2010

칠성초에 역병 저항성을 도입한 칠복1호에 베트남 도입 풋마름병 저항성 계통을 교배하여 육성한 $F_2$ 및 $BC_1F_1$에서부터 $F_4$ 및 $BC_1F_3$까지 역병-풋마름병 복합 저항성 선발을 2009년도와 2010년도에 걸쳐 수행하였다. 매 세대 역병을 접종하여 저항성을 평가하여 선발하고 선발개체에 풋마름병을 접종하여 감염되는 개체는 도태하였다. 역병에 대한 저항성은 선발과 함께 현저히 향상되었으며, 선발계통들은 역병 저항성으로 판매되고 있는 교배종 '무한질주'와 비슷한 수준의 저항성을 나타내었다. $BC_1F_2$ 선발개체를 칠복CMS-A라인에 교배를 하여 $F_1$의 임성을 보고 화분친의 CMS-Rf유전자형을 검정하였다. 대부분Nrfrf로 고정되고, 칠복 ${\times}$ KC995, 칠복 ${\times}$ KC1009 조합의 일부 개체가 이형(heterozygote) 상태인 것으로 확인되었다.

• The Plant Pathology Journal
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• 제30권1호
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• pp.75-81
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• 2014

In total, 170 carrot lines developed in Korea were screened for resistance to Meloidogyne incognita race 1 to select parental genetic resources useful for the development of nematode-resistant carrot cultivars. Using the gall index (GI), gall formation was examined on carrot roots inoculated with approximately 1,000 second-stage juveniles of the nematode 7 weeks after inoculation. Sixty-one carrot lines were resistant (GI ${\leq}1.0$), while the other 109 were susceptible (GI > 1.0) with coefficient of variance (CV) of GI for total carrot lines 0.68, indicating low-variation of GI within the lines examined. The histopathological responses of two carrot plants from resistant and susceptible lines were examined after nematode infection. In susceptible carrots, giant cells formed with no discernible necrosis around the infecting nematodes. In the resistant carrot line, however, no giant cells formed, although modified cells were observed with extensive formation of necrotic layers through their middle lamella and around the infecting nematodes. This suggested that these structural modifications were related to hypersensitive responses governed by the expression of true resistance genes. Therefore, the Korean carrot lines resistant to the nematode infection are potential genetic resources for the development of quality carrot cultivars resistant to M. incognita race 1.

• 한국육종학회지
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• 제41권3호
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• pp.346-348
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• 2009

1997년부터 황색 겹꽃 프리지아 품종 육성을 위한 품종을 수집하여, '98년 2월에 'Golden Crown'을 모본으로 하고 'White Wing'을 부본으로 한 교배조합으로부터 240개의 종자를 획득하였다. 실생으로부터 파종 7개월 후 화색이 선명한 황색겹꽃 우수개체를 1차로 선발하였다. 2000년부터 2004년까지 5년에 걸쳐 생육 특성 및 수량 검정을 실시하였으며 안정성, 균일성, 병 저항성, 절화수명, 및 구근 증식력이 우수하다고 판단되어 2005년 농작물 직무육성 신품종선정위원회의 심의를 거쳐 "Golden Amor"로 명명하였다. "Golden Amor"는 황색 겹꽃 품종으로 꽃잎 내부 반점이 없는 절화용 대형화품종이다. 개화기는 대비품종에 비해 늦고, 화수는 12개 이상으로 많다. 주당 자구수는 9.9개로 대비품종보다 많으며 기호성이 높다.

• 한국육종학회지
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• 제42권4호
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• pp.357-364
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• 2010

In a previous study, we mapped 12 QTLs for 1,000 grain weight (TGW) in the 172 $BC_2F_2$ lines derived from a cross between Oryza sativa ssp. Japonica cv. Hwaseongbyeo and O. rufipogon. These QTLs explained 5.4 - 11.4% of the phenotypic variance for TGW. Marker-aided selection combined with backcrosses was employed to develop QTL-NILs for each QTL. $BC_2F_2$ lines with each target QTL were backcrossed to Hwaseongbyeo twice and then allowed to self to produce $BC_4F_5$ populations. SSR markers linked to TGW were employed to select QTL-NILs with the respective target QTL. Six QTL-NILs with the recurrent parent, Hwaseongbyeo were evaluated for nine traits for three years from 2007 and 2009. Differences were observed between each of the 6 QTL-NILs and Hwaseongbyeo in TGW. In addition to TGW, these QTL-NILs displayed differences in other agronomic traits possibly indicating a tight linkage of genes controlling these traits. The direction of the QTL for TGW in 6 QTL-NILs was consistent as in the $BC_2F_2$ lines from the same cross. Difference in TGW between each of the QTL-NILs and Hwaseongbyeo was associated with the difference in one or two grain shape traits; grain length, grain width, and grain thickness. SSR markers linked to the QTL for TGW will facilitate selection of the grain shape character in a breeding program to diversify grain shape and provide the foundation for map-based gene isolation. Also, the QTL-NILs developed in this report and the progenies from crosses between the QTL-NILs will be useful in clarifying epistatic interactions among QTLs for TGW.

• 한국육종학회지
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• 제41권4호
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• pp.429-436
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• 2009

We conducted a QTL analysis of grain quality traits using 117 $BC_3F_4$ and $BC_3F_5$ lines developed from a cross between Ilpumbyeo and Moroberekan. Genotypes of 117 $BC_3F_5$ lines were determined using 134 simple sequence repeat (SSR) markers. A linkage map constructed using 134 SSR markers was employed to characterize quantitative trait loci (QTL). The 117 $BC_3F_4$ and $BC_3F_5$ lines were evaluated for eleven grain quality traits in 2005 and 2006. A total of 18 QTLs were identified for eleven traits, and the phenotypic variance explained by each QTL ranged from 9.9% to 35.2%. Moroberekan alleles contributed positive effects in the Ilpumbyeo background at two QTL loci for 1,000 grain weight. Four QTLs, two for chalky rice and one each for 1,000 grain weight and head rice were consistently detected in two consecutive years indicating that these QTLs are stable. Clusters of QTLs were observed in three chromosome regions. One cluster harboring five QTLs including head rice and brown rice ratio near SSR markers RM190 and RM314 was detected on chromosome 6. Another cluster harboring grain weight and white belly was detected on chromosome 2. Increase in white belly at this locus might be due to the increase in grain weight due to the presence of the Moroberekan allele. The Moroberekan alleles at two QTL loci, gw3 and gw4 associated with increased grain weight might be useful in breeding programs to develop high-yielding cultivars.

• 한국육종학회지
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• 제42권1호
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• pp.35-39
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• 2010

Soybean proteins are widely used for human and animal feed in the world. ${\beta}$-conglycinin protein exhibits poor nutritional and food processing properties and Kunitz trypsin inhibitor (KTI) protein is a main anti-nutritional factor in soybean seed. The objective of this research was to identify the inheritance of $cgy_1$ gene and ti gene for the improvement of soybean cultivar with no KTI proteins and low amount of ${\beta}$-conglycinin. $F_2$ population was made by crossing between "Gaechuck2ho" (${\alpha}^{\prime}$-subunit present $Cgy_1Cgy_1$, KTI protein absent titi) and PI506876 (${\alpha}^{\prime}$-subunit absent $cgy_1cgy_1$, KTI protein present TiTi) parent. A total of 434 $F_2$ seeds were obtained and analyzed for the segregation of ${\alpha}^{\prime}$-subunit protein and KTI protein using SDS-PAGE. The segregation ratio of 3 : 1 for $Cgy_1$ locus (310 $Cgy_1$_ : 124 $cgy_1cgy_1$) and Ti locus (339 Ti_ : 95 titi) were observed. Segregation ratios of 9 : 3 : 3 : 1 (241 $Cgy_1$_Ti_: 69 $Cgy_1$_titi: 98 $cgy_1cgy_1$Ti_: 26 $cgy_1cgy_1titi$) between $Cgy_1$ gene and Ti gene in $F_2$ seeds were also observed (${\chi}^2= 5.367$, P = 0.10 - 0.20). This data showed that $Cgy_1$ gene was inherited independently with the Ti gene in soybean. These results will be useful in breeding program for selecting the line that does not exhibit or lacks both ${\alpha}^{\prime}$-subunit protein and KTI protein in soybean.

• 한국육종학회지
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• 제40권2호
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• pp.201-205
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• 2008

고구마는 주로 식용으로 이용되고 있지만, 당면 제빵 제과와 같은 식품가공용이나 에탄올 등 공업원료용 그리고 지상부의 특성을 이용한 관상용으로도 이용이 가능하다. 본 연구는 고구마의 잎색이나 잎모양, 개화성 등 지상부의 다양한 특성을 이용한 관상용 고구마를 선발하여 고구마의 이용도를 높이고자 하였다. 식용으로 재배되는 고구마는 극심한 한발이나 저온과 같은 주위 환경의 영향으로 드물게 꽃이 피는 경우도 있지만 일반적으로 꽃이 피지 않는다. 그러나 개화성 고구마 '모닝퍼플'은 일장에 무관하게 줄기가 자라면서 액아에서 화총이 형성되어 계속적으로 개화하는 특성이 있다. '모닝퍼플'은 화기특성으로 화색은 담자색이고, 꽃잎크기는 4.5 cm, 꽃부리통은 0.9 cm, 꽃길이는 4 cm, 화판 모양은 8각형이다. 지상부 특성으로 줄기길이는 186 cm, 줄기색은 녹색, 잎모양은 단결각, 잎자루색은 녹색을 띠고 있다. 개화특성으로 개화 기간은 90일, 주당화수는 108개, 꽃자루수는 95개, 꽃자루길이는 5~1 cm, 꽃받침은 5개이다. 모닝퍼플은 실내 및 도로변 화분에 식재하여 관상용으로 이용이 가능하다.