Korean Journal of Breeding Science (한국육종학회지)

The Korean Society of Breeding Science (한국육종학회)
권호 표지

Development and Evaluation of QTL-NILs for Grain Weight from an Interspecific Cross in Rice

  • Yun, Yeo-Tae (Chungnam Agricultural Research and Extension Services) ;
  • Kim, Dong-Min (Department of Agronomy, Chungnam National University) ;
  • Park, In-Kyu (Department of Agronomy, Chungnam National University) ;
  • Chung, Chong-Tae (Chungnam Agricultural Research and Extension Services) ;
  • Seong, Yeaul-Kyu (Chungnam Agricultural Research and Extension Services) ;
  • Ahn, Sang-Nag (Department of Agronomy, Chungnam National University)
  • Received : 2010.09.13
  • Published : 2010.09.30
⟨ Previous Next ⟩

Abstract

In a previous study, we mapped 12 QTLs for 1,000 grain weight (TGW) in the 172 $BC_2F_2$ lines derived from a cross between Oryza sativa ssp. Japonica cv. Hwaseongbyeo and O. rufipogon. These QTLs explained 5.4 - 11.4% of the phenotypic variance for TGW. Marker-aided selection combined with backcrosses was employed to develop QTL-NILs for each QTL. $BC_2F_2$ lines with each target QTL were backcrossed to Hwaseongbyeo twice and then allowed to self to produce $BC_4F_5$ populations. SSR markers linked to TGW were employed to select QTL-NILs with the respective target QTL. Six QTL-NILs with the recurrent parent, Hwaseongbyeo were evaluated for nine traits for three years from 2007 and 2009. Differences were observed between each of the 6 QTL-NILs and Hwaseongbyeo in TGW. In addition to TGW, these QTL-NILs displayed differences in other agronomic traits possibly indicating a tight linkage of genes controlling these traits. The direction of the QTL for TGW in 6 QTL-NILs was consistent as in the $BC_2F_2$ lines from the same cross. Difference in TGW between each of the QTL-NILs and Hwaseongbyeo was associated with the difference in one or two grain shape traits; grain length, grain width, and grain thickness. SSR markers linked to the QTL for TGW will facilitate selection of the grain shape character in a breeding program to diversify grain shape and provide the foundation for map-based gene isolation. Also, the QTL-NILs developed in this report and the progenies from crosses between the QTL-NILs will be useful in clarifying epistatic interactions among QTLs for TGW.

Keywords

Acknowledgement

Supported by : RDA

References

  1. Causse MA, Fulton TM, Cho YG, Ahn SN, Chunwongse J, Wu K, Xiao J, Yu Z, Ronald PC, Harrington SE, Second G, McCouch SR, Tanksley SD. 1994. Saturated molecular map of the rice genome based on an interspecific backcross population. Genetics 138: 1251-1274.
  2. Cho YC, Suh JP, Choi IS, Hong HC, Baek MK, Kang KH, Kim YG, Ahn SN, Choi HC, Hwang HG, Moon HP. 2003. QTLs analysis of yield and its related traits in wild rice relative Oryza rufipogon. Treat. Crop Res. 4: 19-29.
  3. Fan C, Xing YZ, Mao HL, Lu TT, Han B, Xu C, Li XH, Zhang Q. 2006. GS3, a major QTL for grain length and weight and minor QTL for grain width and thickness in rice, encodes a putative transmembrane protein. Theor. Appl. Genet. 112: 1164-1171. https://doi.org/10.1007/s00122-006-0218-1
  4. Hirota O, Oka M, Takeda T. 1990. Sink activity estimation by sink size and dry matter increase during the ripening stage of barley (Hordeum vulgare) and rice (Oryza sativa). Ann. Bot. 65, 349-354. https://doi.org/10.1093/oxfordjournals.aob.a087944
  5. Kinoshita T. 1998. Report of the committee on gene symbolization, nomenclature and linkage groups. II. Linkage mapping using mutant genes in rice. Rice Genet. Newsl. 15: 13-74.
  6. Kubo T, Takanokai N, Yoshimura A. 2001. RFLP mapping of genes for long kernel and awn on chromosome 3 in rice. Rice Genet. Newsl. 18: 26-28.
  7. Li JM, Thomson M, McCouch SR. 2004. Fine mapping of a grain-weight quantitative trait locus in the pericentromeric region of rice chromosome 3. Genetics 168: 2187-2195. https://doi.org/10.1534/genetics.104.034165
  8. Lin HX, Yamamoto T, Sasaki T, Yano M. 2000. Characterization and detection of epistatic interactions of three QTLs, Hd1, Hd2 and Hd3, controlling heading date in rice using nearly isogenic lines. Theor. Appl. Genet. 101:1021-1028. https://doi.org/10.1007/s001220051576
  9. McCouch SR, Teytelman L, Xu Y, Lobos KB, Clare K, Walton M, Fu B, Maghirang R, Li Z, Xing Y, Zhang Q, Kono I, Yano M, Fjellstrom R, DeClerck G, Schneider D, Cartinhour S, Ware D, Stein L. 2002. Development and mapping of 2240 new SSR markers for rice (Oryza sativa L). DNA Res. 9:199–207
  10. Mei HW, Li ZK, Shu QY, Guo LB, Wang YP, Yu XQ, Ying CS, Luo LJ. 2005. Gene actions of QTL affecting several agronomic traits resolved in a recombinant inbred rice population and two backcross population. Theor. Appl. Genet. 110: 649-659. https://doi.org/10.1007/s00122-004-1890-7
  11. Panaud O, Chen X, McCouch SR. 1996. Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in Rice (Oryza sativa L.). Mol. Gen. Genet. 252: 597-607.
  12. Shomura A, Izawa T, Ebana K, Ebitan T, Kanegae H, Konishi S, Yano M. 2008. Deletion in a gene associated with grain size increased yields during rice domestication. Nat. Genet. 40: 1023-1028. https://doi.org/10.1038/ng.169
  13. Song XJ, Huang W, Shi M, Zhu MZ, Lin HX. 2007. A QTL for rice grain width and weight encodes a previously unknown RING-type E3 ubiquitin ligase. Nat. Genet. 39:623-630. https://doi.org/10.1038/ng2014
  14. Temnykh S, Park WD, Ayres N, Cartinhour S, Hauck N, Lipovich L, Cho YG, Ishii T, McCouch SR. 2000. Mapping and genome organization of microsatellite sequences in rice (Oryza sativa L.). Theor. Appl. Genet. 100:698-712.
  15. Thomson MJ, Tai TH, McClung AM, Lai XH, Hinga ME, Lobos KB, Xu Y, Martinez CP, McCouch SR. 2003. Mapping quantitative trait loci for yield components and morphological traits in an advanced backcross population between Oryza rufipogon and the Oryza sativa cultivar Jefferson. Theor. Appl. Genet. 107: 479-493. https://doi.org/10.1007/s00122-003-1270-8
  16. Wan XY, Wan JM, Weng JF, Jiang L, Bi JC, Wang CM, Zhai HQ. 2005. Stability of QTLs for rice grain dimension and endosperm chalkiness characteristics across eight environments. Theor. Appl. Genet. 110: 1334-1346. https://doi.org/10.1007/s00122-005-1976-x
  17. Wang E, Wang J, Zhu X, Hao W, Wang L, Li Q, Zhang L, He W, Lu B, Lin H, Ma H, Zhang G, He Z. 2008. Control of rice grain-filling and yield by a gene with a potential signature of domestication. Nat. Genet. 40: 1370-1374. https://doi.org/10.1038/ng.220
  18. Xie X, Song MH, Jin F, Ahn SN, Suh JP, Hwang HG, McCouch SR. 2006. Fine mapping a grain weight quantitative trait loci on rice chromosome 8 using nearly-isogenic lines derived from a cross between Oryza sativa and O. rufipogon. Theor. Appl. Genet. 113: 885-894. https://doi.org/10.1007/s00122-006-0348-5
  19. Xie X, Jin F, Song MH, Suh JP, Hwang HG, Kim YG, McCouch SR, Ahn SN. 2008. Fine mapping a yieldenhancing QTL cluster associated with transgression variation in an Oryza sativa and O. rufipogon cross. Theor. Appl. Genet. 116: 613-622. https://doi.org/10.1007/s00122-007-0695-x
  20. Yamamoto T, Lin HX, Sasaki T, Yano M. 2000. Identification of heading date quantitative trait locus Hd6 and characterization of its epistatic interactions with Hd2 in rice using advanced backcross progeny. Genetics 154:885-891.
  21. Yamamoto T, Yonemaru J, Yano M. 2009. Towards the understanding of complex traits in rice: substantially or supercially?. DNA Res. 16: 141-154. https://doi.org/10.1093/dnares/dsp006
  22. Yano M, Sasaki T. 1997. Genetic and molecular dissection of quantitative traits in rice. Plant Mol. Biol. 35:145-153. https://doi.org/10.1023/A:1005764209331
  23. Yoon DB, Kang KH, Kim HJ, JU HG, Kwon SJ, Suh JP, Jeong OY, Ahn SN. 2006. Mapping quantitative trait loci for yield components and morphological traits in an advanced backcross population between Oryza grandiglumis and the O. sativa japonica cultivar Hwaseongbyeo. Theor. Appl. Genet. 112: 1052-1062. https://doi.org/10.1007/s00122-006-0207-4
  24. Zhou L, Chen L, Jiang L, Zhang W, Liu L, Liu X, Zhao Z, Zhang L, Wang J, Wan J. 2009. Fine mapping of the grain chalkiness QTL qPGWC-7 in rice (Oryza sativa L.). Theor. Appl. Genet. 118: 581-590. https://doi.org/10.1007/s00122-008-0922-0