Korean Journal of Breeding Science (한국육종학회지)

The Korean Society of Breeding Science (한국육종학회)
권호 표지

Inheritance of Cgy1 gene and Ti gene in Mature Soybean Seed

  • Sung, Mi-Kyung (Department of Agronomy, Research Institute of Life Science, Gyeongsang National University) ;
  • Han, Eun-Hui (Department of Agronomy, Research Institute of Life Science, Gyeongsang National University) ;
  • Kim, Kyung-Roc (Department of Agronomy, Research Institute of Life Science, Gyeongsang National University) ;
  • Park, Jung-Soo (Department of Agronomy, Research Institute of Life Science, Gyeongsang National University) ;
  • Hwang, Kyo-Jin (Department of Agronomy, Research Institute of Life Science, Gyeongsang National University) ;
  • Nam, Jin-Woo (Department of Agronomy, Research Institute of Life Science, Gyeongsang National University) ;
  • Chung, Jong-Il (Department of Agronomy, Research Institute of Life Science, Gyeongsang National University)
  • Received : 2010.03.15
  • Published : 20100300
⟨ Previous Next ⟩

Abstract

Soybean proteins are widely used for human and animal feed in the world. ${\beta}$-conglycinin protein exhibits poor nutritional and food processing properties and Kunitz trypsin inhibitor (KTI) protein is a main anti-nutritional factor in soybean seed. The objective of this research was to identify the inheritance of $cgy_1$ gene and ti gene for the improvement of soybean cultivar with no KTI proteins and low amount of ${\beta}$-conglycinin. $F_2$ population was made by crossing between "Gaechuck2ho" (${\alpha}^{\prime}$-subunit present $Cgy_1Cgy_1$, KTI protein absent titi) and PI506876 (${\alpha}^{\prime}$-subunit absent $cgy_1cgy_1$, KTI protein present TiTi) parent. A total of 434 $F_2$ seeds were obtained and analyzed for the segregation of ${\alpha}^{\prime}$-subunit protein and KTI protein using SDS-PAGE. The segregation ratio of 3 : 1 for $Cgy_1$ locus (310 $Cgy_1$_ : 124 $cgy_1cgy_1$) and Ti locus (339 Ti_ : 95 titi) were observed. Segregation ratios of 9 : 3 : 3 : 1 (241 $Cgy_1$_Ti_: 69 $Cgy_1$_titi: 98 $cgy_1cgy_1$Ti_: 26 $cgy_1cgy_1titi$) between $Cgy_1$ gene and Ti gene in $F_2$ seeds were also observed (${\chi}^2= 5.367$, P = 0.10 - 0.20). This data showed that $Cgy_1$ gene was inherited independently with the Ti gene in soybean. These results will be useful in breeding program for selecting the line that does not exhibit or lacks both ${\alpha}^{\prime}$-subunit protein and KTI protein in soybean.

Keywords

Acknowledgement

Supported by : Functional Genomics Center (CFGC)

References

  1. Birk Y (1961). Purification and some properties of a highly active trypsin and chymotrypsin from soybeans. Biochem.Acta 54:378-381. https://doi.org/10.1016/0006-3002(61)90387-0
  2. Chen Z, Shoemaker RC (1998). Four genes affecting seed traits in soybeans map 10 linkage group F. The J. of Heredity 89(3):211-215. https://doi.org/10.1093/jhered/89.3.211
  3. Cregan PB, Jarvik T, Bush AL, Shoemaker RC, Lark KG, Kahler AL, KayaN, VanToai TT, Lohnes DG, Chung JI, Specht JE (1999). An integrated genetic linkage map of the soybean. Crop Sci.39:1464-1490. https://doi.org/10.2135/cropsci1999.3951464x
  4. Frattali V, Steiner RF (1968). Soybean inhibitor. I. Separationssome properties of three inhibitors from commercial crudetrypsin inhibitors. Biochem. 7:521-530. https://doi.org/10.1021/bi00842a006
  5. Hyrnowitz T, Hadley HH (1972). Inheritance of a trypsin variant in seed protein of soybeans. Crop Sci. 12:197-198. https://doi.org/10.2135/cropsci1972.0011183X001200020013x
  6. Hildebrand DF, Orf JH, Hymowitz T (1980). Inheritance of anacid phosphatase and its linkage with the Kunitz trypsin inhibitor seed protein of soybeans. Crop Sci. 20:83-85. https://doi.org/10.2135/cropsci1980.0011183X002000010019x
  7. Hajika M, Takahashi M, Sakai S, Matsunaga R (1998). Dominant inheritance of a trait lacking ${\beta}-conglycinin$ detected in a wild soybean line. Breeding Science 48:383-386.
  8. Hayashi M, Nishioka M, Kitamura K, Harada K (2000). Identification of AFLP markers lightly linked to thc gene for deficiency of the 7S globulin in soybean iced and characterization of abnormal phenotypes involved in the mutation. Breeding Science 50:123-129. https://doi.org/10.1270/jsbbs.50.123
  9. Kunitz M (1945). Crystallization of a trypsin inhibitor from soybean. Science 101:668-669. https://doi.org/10.1126/science.101.2635.668
  10. Kiang YT (1987). Mapping three protein loci on a soybean chromosome. Crop Sci. 27:44-46. https://doi.org/10.2135/cropsci1987.0011183X002700010011x
  11. Kim MS, Park MJ, Jeong WH, Nam KC, Chung JI (2006). SSRmarker tightly linked to the Ti locus in soybean [Glycine max (L.) Merr.]. Euphytica 152:361-366. https://doi.org/10.1007/s10681-006-9223-3
  12. Koshiyama I (1968). Chemical and physical properties in soybean globulins. Cereal Chem. 45:394-404.
  13. Kitamura K, Kaizuma N (1981). Mutant strains with low level of subunits of 7S globulins in soybean seed. Japan J. Breed. 31:353-359.
  14. Kitamura K, Davies CS, Nielsen NC (1984). Inheritance of alleles for $Cgy_1$ and $Gy_4$ storage protein genes in soybean. Theor. Appl. Genet. 68:253-257. https://doi.org/10.1007/BF00266899
  15. Lee KJ, Park MS, Sung MK, Kim MS, Chung JI (2008). Inheritance between Le gene and Ti gene in soybean (Glycine max L.). Korean J. Breed. Sci. 40(2): 97-100.
  16. Orf JH, Hymowitz T (1979). Inheritance of the absence of the trypsin inhibitor in seed protein of soybeans. Crop Sci. 19:107-109. https://doi.org/10.2135/cropsci1979.0011183X001900010026x
  17. Ogawa TE, Tayama E, Kitamura K, Kaizuma N (1989). Genetic improvement of seed storage proteins using three variant alleles of 7S globulin subunits in soybean. Japan J. Breed. 39:137-147.
  18. Rackis JJ, Sasame HA, Mann RR, Anderson RL, Smith AK (1962). Soybean trypsin inhibitors: Isolation, purification and properties. Biochem. Biophys. 98:471-478. https://doi.org/10.1016/0003-9861(62)90213-8
  19. Singh L, Wilson CM, Hooky HH (1969). Genetic differences in soybean trypsin inhibitors separated by disc electrophoresis. Crop Sci. 9:489-491. https://doi.org/10.2135/cropsci1969.0011183X000900040031x
  20. Thanh VH, Shibasaki K (1976). Heterogeneity of beta-conglycinin. Biochim. Biophys. Acta. 469:326-328.
  21. Takahashi K, Mizuno Y, Yumoto S, Kitamura K, Nakamllra S (1996). Inheritance of the ${\alpha}-subunit$ deficiency of ${\beta}-conglycinin in soybean (Glycine max M.) line induced by gamma-ray irradiation. Breeding Science 46:251-255.