Korean Journal of Breeding Science (한국육종학회지)

The Korean Society of Breeding Science (한국육종학회)
권호 표지

Mapping QTL for Grain Quality Traits Using an Introgression Line Population from a Cross between Ilpumbyeo and Moroberekan in Rice

일품벼/모로베레칸 이입계통을 이용한 미질특성 관련 QTL 분석

  • Ju, Hong-Guang (College of Agriculture & Life Sciences, Chungnam National University) ;
  • Kim, Dong-Min (College of Agriculture & Life Sciences, Chungnam National University) ;
  • Oh, Chang-Sik (College of Agriculture & Life Sciences, Chungnam National University) ;
  • Kim, Myung-Ki (Rice Research Division, National Institute of Crop Science) ;
  • Kim, Kee-Jong (Rice Research Division, National Institute of Crop Science) ;
  • Ahn, Sang-Nag (College of Agriculture & Life Sciences, Chungnam National University)
  • 구홍광 (충남대학교 농업생명과학대학) ;
  • 김동민 (충남대학교 농업생명과학대학) ;
  • 오창식 (충남대학교 농업생명과학대학) ;
  • 김명기 (국립식량과학원 답작과) ;
  • 김기종 (국립식량과학원 답작과) ;
  • 안상낙 (충남대학교 농업생명과학대학)
  • Received : 2009.11.03
  • Published : 20091200
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Abstract

We conducted a QTL analysis of grain quality traits using 117 $BC_3F_4$ and $BC_3F_5$ lines developed from a cross between Ilpumbyeo and Moroberekan. Genotypes of 117 $BC_3F_5$ lines were determined using 134 simple sequence repeat (SSR) markers. A linkage map constructed using 134 SSR markers was employed to characterize quantitative trait loci (QTL). The 117 $BC_3F_4$ and $BC_3F_5$ lines were evaluated for eleven grain quality traits in 2005 and 2006. A total of 18 QTLs were identified for eleven traits, and the phenotypic variance explained by each QTL ranged from 9.9% to 35.2%. Moroberekan alleles contributed positive effects in the Ilpumbyeo background at two QTL loci for 1,000 grain weight. Four QTLs, two for chalky rice and one each for 1,000 grain weight and head rice were consistently detected in two consecutive years indicating that these QTLs are stable. Clusters of QTLs were observed in three chromosome regions. One cluster harboring five QTLs including head rice and brown rice ratio near SSR markers RM190 and RM314 was detected on chromosome 6. Another cluster harboring grain weight and white belly was detected on chromosome 2. Increase in white belly at this locus might be due to the increase in grain weight due to the presence of the Moroberekan allele. The Moroberekan alleles at two QTL loci, gw3 and gw4 associated with increased grain weight might be useful in breeding programs to develop high-yielding cultivars.

Keywords

Acknowledgement

Supported by : 작물기능유전체사업단

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