• Title/Summary/Keyword: biological stability

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A Faunistic Study of Insects of Uninhabited Islands in the Docho-myeon, Sinan-gun, Jeollanam-do, Korea (전라남도 신안군 도초면 일대 무인도서의 곤충상)

  • Cho, Young-Ho;Kim, Young-Jin;Lim, Heon-Myoung;Han, Yong-Gu;Choi, Min-Joo;Nam, Sang-Ho
    • Korean Journal of Environment and Ecology
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    • v.25 no.5
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    • pp.673-684
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    • 2011
  • Shinan-gun in Jeollanam-do has 1,004 islands which is the biggest number in the whole country. As most of the islands in Korea are secluded from the mainland or other islands, biological surveys are very important for the biogeographical perspective. Especially, the species diversity of insecta on an island plays an important role in the food stability of top predators, including birds. This study provides baseline academic resources for the preservation and management of uninhabited islands by providing information about the status of insects on the uninhabited islands in Docho-myeon, Jeollanam-do, Korea. As far as the appearance aspects of the insects in 28 uninhabited islands were 921 individuals, 122 species, 60 families or 12 orders. Ga Island showed the highest appearance as 32 species, 22 families or 9 orders. Appearance of specific insects was investigated unknown species: 1 species, southern characteristic species: 3 species, coastal dune species: 1 species, climate-sensitive indicator species: 1 species.

Isolation of New CHO Cell Mutants Defective in CMP-Sialic Acid Biosynthesis and Transport

  • Shin, Dong-Jun;Kang, Ji Young;Kim, Youn Uck;Yoon, Joong Sik;Choy, Hyon E;Maeda, Yusuke;Kinoshita, Taroh;Hong, Yeongjin
    • Molecules and Cells
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    • v.22 no.3
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    • pp.343-352
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    • 2006
  • Sialic acid is a sugar typically found at the N-glycan termini of glycoproteins in mammalian cells. Lec3 CHO cell mutants are deficient in epimerase activity, due to a defect in the gene that encodes a bifunctional UDP-GlcNAc 2-epimerase/ManNAc kinase (GNE). Sialic acid modification on the cell surface is partially affected in these cells. We have mutagenized Lec3 CHO cells and isolated six mutants (termed C2m) deficient in the cell surface expression of polysialic acid (PSA). Mutant C2m9 was partially defective in expression of cell-surface PSA and wheat germ agglutinin (WGA) binding, while in the other five mutants, both cell-surface PSA and WGA binding were undetectable. PSA expression was restored by complementation with the gene encoding the CMP-sialic acid transporter (CST), indicating that CST mutations were responsible for the phenotypes of the C2m cells. We characterized the CST mutations in these cells by Northern blotting and RT-PCR. C2m9 and C2m45 carried missense mutations resulting in glycine to glutamate substitutions at amino acids 217 (G217E) and 256 (G256E), respectively. C2m13, C2m39 and C2m31 had nonsense mutations that resulted in decreased CST mRNA stability, and C2m34 carried a putative splice site mutation. PSA and CD15s expression in CST-deficient Lec2 cells were partially rescued by G217E CST, but not by G256E CST, although both proteins were expressed at similar levels, and localized to the Golgi. These results indicate that the novel missense mutations isolated in this study affect CST activity.

Evaluation for Optimal HUD Location on a Train Using EEG (뇌파를 이용한 열차 최적의 HUD위치 평가)

  • Wang, Chang-Won;Kim, Yong-Kyu;Min, Se-Dong
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.39C no.11
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    • pp.985-993
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    • 2014
  • This paper suggested a new evaluation method for optimal HUD position through a correlation based on between biological signal as and statistical analysis which using (Electroencephalogram, EEG) and ANOVA. This experiment was conducted two kinds of method to evaluate the optimal HUD position. At first, visual stimulus suggested from six different positions(the top and the bottom of the left, the top and the bottom of the center and the top and the bottom of the right on the screen) in laboratory and an object image was shown for 30 seconds in a screen which has $235{\times}197cm2$ size. And second, HUD image was configured from three different positions and an object image was shown for 30 seconds in a screen. EEG, which used ${\alpha}$-wave and ${\beta}$-wave for evaluate an emotional stability, were measured from Fp1, Fp2, F7 and F8 channel based on ten to twenty electrode system. From the result in laboratory, F7 ${\beta}$-wave was shown statistically significant to significance probability of 0.006 and between ${\alpha}$-wave and ${\beta}$-wave were showed a negative correlation(r=-0.190). Also, Both the top of left and the bottom of center were showed lower ${\beta}$-wave than the bottom of right. From the result in railway simulator, Fp1 ${\beta}$-wave was appeared statistically significant as significance probability of 0.033 and it was showed lower ${\beta}$-wave than center. The outcome of this study will be helpful about evaluation of optimal HUD position through correlation between alpha wave and beta wave.

Dewatering Filtrate Treatment with Center Well Depth of Secondary Clarifier in Small Sewage Treatment Plant (소규모하수처리시설의 이차침전조 내통길이 변화를 통한 탈수여액의 처리)

  • Choi, Jung-Su;Kim, Hyun-Gu;Lee, Dong-Ho;Joo, Hyun-Jong
    • Journal of Korean Society of Environmental Engineers
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    • v.35 no.10
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    • pp.694-702
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    • 2013
  • The purpose of this study is to evaluate a de-watering filtrate treatment and the possibility of securing biological treatment capacity by changing the structure of the secondary clarifier. Accordingly, the column test was conducted to determine the effect of polymer in the de-watering filtrate on sludge sedimentation. Also, the characteristics of de-watering filtrate processing was evaluated through batch test and continuous processing operation. The results showed that sludge settling velocity increased with higher polymer concentration, and that effluent SS concentration was found to decrease. Regarding processing characteristics of de-watering filtrate, the removal efficiency of TSS and TBOD5 increased as the length of secondary clarifier was longer. Also, comparing injections into anoxic tank and secondary clarifier, de-watering filtrate by continuous infusion treatment process showed stability in both conditions. Therefore, by modifying the structure of secondary clarifier, efficient processing of de-watering filtrate is expected to be possible and processing capacity of small sewage treatment plants is considered to be improved.

Cold Pasteurization of Frozen Crushed Garlics using Electron Beam Irradiation and the Stability of Bioactive Components (냉동다진마늘에 대한 전자선 살균 효과와 생리활성 성분의 안정성)

  • Lee, Hyun-Gyu;Jo, Yunhee;Kwon, Joong-Ho
    • Korean Journal of Food Science and Technology
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    • v.48 no.1
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    • pp.9-14
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    • 2016
  • Recently, the market for ready-to-use vegetables has increased largely due to consumer demands, which led to the production of minimally-processed frozen crushed garlic products. This study was designed to determine the effect of electron beam irradiation (0-7 kGy) on microbial decontamination, bioactive compounds, and antimicrobial activities of frozen crushed garlic obtained from Korea and China. The microbial counts (total bacteria, yeasts & molds) were reduced from log 3-4 CFU/g to non-detectable levels as a result of irradiation at 4 kGy. Irradiation treatment at 4 kGy did not affect the amount of alliin, allicin, total pyruvate, and total thiosulfinate of crushed garlic, however, this dose induced insignificant changes in antibacterial activities against pathogenic bacteria including Bacillus subtilis, Staphylococcus aureus, Salmonella enteritidis, Vibrio parahaemolyticus, and Yersinia enterocolitica. Therefore, electron beam irradiation less than 4 kGy can be considered suitable to improve the microbial decontamination without altering the biological activity of frozen garlics.

Isolation and characterization of bacteriophages for the control of Shiga Toxin-producing E. coli (시가 독소 생성 대장균의 제어를 위한 박테리오파지의 분리와 특성 분석)

  • Lim, Ga-Yeon;Park, Do Won;Lee, Young-Duck;Park, Jong-Hyun
    • Korean Journal of Food Science and Technology
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    • v.50 no.6
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    • pp.594-600
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    • 2018
  • Shiga toxin-producing Escherichia coli (STEC) is an important pathogenic bacterium. To control STEC, the characteristics of the ECP33 and NOECP91 coliphages, which belong to the Myoviridae family, were analyzed. The host inhibition range for a total of 44 STEC strains was 45.5% for ECP33 and 65.9% for NOECP91. ECP33 and NOECP91 were relatively stable at $65^{\circ}C$, 50 ppm of sodium hyperchlorite, and a pH value of 4-10. However, the two phages were susceptible to a temperature of $70^{\circ}C$. NOECP91 was killed within 1 h after exposure to 30% ethanol, but ECP33 showed high tolerance even after exposure to 70% ethanol for 1 h. Interestingly, the inhibition of STEC growth according to the multiplicity of infection of 0.1 was confirmed until no growth was observed after 10 hours of culture with the phages. Therefore, the ECP33 and NOECP91 phages may be applied as a biological control agent for Shiga toxin-producing E. coli.

Assessments of Dissolved Rare Earth Elements and Anthropogenic Gadolinium Concentrations in Different Processes of Wastewater Treatment Plant in Busan, Korea (부산 하수처리장에서 공정별 용존 희토류 원소의 농도 및 인위적 기원 가돌리늄의 배출량 평가)

  • Lim, Ijin;Ryu, Jong-Sik;Lee, Joonyeob;Lee, Jun-Ho;Cho, Hyung-Mi;Kim, Taejin
    • Journal of the Korean earth science society
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    • v.43 no.2
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    • pp.303-311
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    • 2022
  • Gadolinium, commonly used as a contrast agent for magnetic resonance imaging (MRI), is discharged into aquatic environments without removal after treatment in wastewater treatment plants (WWTPs) because of its high stability. In this study, we collected water samples from Suyeong WWTP, Busan, to investigate the dissolved rare earth element (REE) removal capacity of each wastewater treatment process and to evaluate the discharge of anthropogenic Gd (Gdanth) from effluents. As wastewater passed through each stage of treatment, the concentrations of light REEs (La-Eu) decreased, whereas those of heavy REEs (Tb-Lu) were relatively consistent. Negative Sm anomalies (<1) were observed in several samples, indicating that Sm can be removed by adsorption onto particles or phosphate during the biological removal process. Positive Gd anomalies (149±50, n=9) were observed in all samples. The ratios of Gdanth concentrations to measured Gd concentrations in all wastewater treatment processes were higher than 97%. This indicates that Gdanth was discharged to the Suyeong River without removal during the wastewater treatment process. Considering the daily treatment capacity in each process, the total flux of Gdanth was estimated to be 259 mmol/day. Our results suggest that mid- and/or long-term monitoring of Gd is needed because Gdanth is continuously discharged into Suyeong Bay through WWTPs.

Mini-implant with additional retentive structure by using digital method (부가적인 유지구조를 가진 미니 임플란트의 디지털 수복 증례)

  • Hwang, Su-Hyun;Bae, Eun-Bin;Cho, Won-Tak;Huh, Jung-Bo
    • The Journal of Korean Academy of Prosthodontics
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    • v.60 no.1
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    • pp.119-126
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    • 2022
  • Mini-implant has been reported as an alternative method to conventional diameter implants in the treatment of mandibular anterior region with narrow ridge. However, one-piece type mini-implant showed biological and technical complications, such as peri-implantitis by residual cement and prosthetic detachment. Recently, one-piece type mini-implant with additional retentive structure has been introduced to increase the retention and stability of the mini-implant. The mini-implant is based on spreading the upper retentive structure with a spreader and connected it with the inner surface of the zirconia prosthesis. In two cases, we tried to reduce these complications through the mini-implant on the narrow mandibular anterior region, and it was possible to reduce the overall treatment period through a digital system. In this case report, restoration using the digital mini-implant system showed improvement of patient satisfaction on the aspect of function and esthetic in the narrow mandibular anterior region.

A Study on Area-Wide Integrated Termite Management for the Preservation of Wooden Built Heritage (목조건축문화재의 예방 보존을 위한 공간적 통합 흰개미 관리(AW-ITM)의 적용)

  • KIM, Sihyun;CHUNG, Yongjae
    • Korean Journal of Heritage: History & Science
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    • v.55 no.3
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    • pp.60-72
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    • 2022
  • A number of wooden built heritage remain in Korea, and most have been damaged by various biological factors including termite. Owing to the irreversible damage caused by termites, wooden built heritage are losing their authenticity and structural stability. In this study, Area-Wide Integrated Termite Management(AW-ITM) was proposed to prevent termite damage. First, to understand the locational characteristics of these sites, the distance from adjacent forests and surrounding forest areas was analyzed for 182 national designated wooden built heritage(national treasures, treasures) using the Geographic Information System(GIS). By analyzing existing pest control projects(2003-2020) and the components of the ITM, the characteristics of termite control for cultural heritages were determined. Based on these results, the cultural heritage sites and their surrounding spaces were divided into three areas, and the types of cultural properties were divided into six types according to the location and number of buildings. Along with this, termite control measures were proposed for each area and type. The concept of AW-ITM has been partially applied to the "Comprehensive Control of Termites in wooden built heritages Sites" by the Cultural Heritage Administration. Caution must be taken with regard to the establishment of a cultural heritage management policy; AW-ITM should be applied on a trial basis with the results then being carefully analyzed and reflected in the establishment of policies pertaining to the conservation management of cultural heritage.

Antioxidant, Anti-thrombosis, and Lipid Accumulation Inhibition Activities of Different Dried Vinegars (3종 분말식초의 항산화, 항혈전 및 항비만 활성)

  • Jong-Kyu Lee;Jong-Sik Kim;Ho-Yong Sohn
    • Journal of Life Science
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    • v.34 no.5
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    • pp.296-303
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    • 2024
  • Vinegar is a fermented food product created by fermenting various sugar- and starch-containing ingredients with microorganisms. It contains a variety of organic acids, sugars, amino acids, esters, and other compounds that contribute to its unique sensory properties. Vinegar is known for its potential benefits, including aiding digestion, lowering blood sugar levels, anti-obesity effects, and antioxidant properties. It is also believed to contribute to improving alkaline body conditions. This study was conducted to develop functional dried vinegar powder from naturally fermented vinegars. Unripe apple, brown rice, and black chokeberry (aronia) were fermented using Gluconacetobacter xylinus for 90-180 days. The filtrate vinegar was spray dried with 37.46% maltodextrin, 5% glucose, 1% citric acid, and 0.04% vitamin C. Analysis of the acidity, color difference, water and soluble solid content, and heat stability of dried vinegar (DV) confirmed that spray drying is a suitable method for powder production. Moreover, the DVs exhibited excellent sensory attributes and solubility. Among the DVs, aronia-DV showed the highest 1,1-diphenyl-2-picryl hydrazyl and 2,2-azobis (3- ethylbenzothiazoline-6-sulfonate) radical scavenging activity (36.7% and 75.3%) and reducing power (0.334) at 0.5 mg/ml concentration, respectively. The nitrite scavenging activity was highest in brown unripe apple-DV, followed by aronia-DV and brown rice-DV. In the anti-thrombosis activity assay, aronia-DV showed the highest prothrombin inhibition. The brown rice-DV exhibited lipid accumulation inhibitory activity in 3T3-L1 adipocytes without cell cytotoxicity. Our results suggest the potential for commercialization of dried vinegar, highlighting its diverse benefits and applications.