Browse > Article
http://dx.doi.org/10.9721/KJFST.2018.50.6.594

Isolation and characterization of bacteriophages for the control of Shiga Toxin-producing E. coli  

Lim, Ga-Yeon (Department of Food Science and Biotechnology, College of BioNano Technology, Gachon University)
Park, Do Won (Department of Food Science and Biotechnology, College of BioNano Technology, Gachon University)
Lee, Young-Duck (Department of Food Science and Engineering, Seowon University)
Park, Jong-Hyun (Department of Food Science and Biotechnology, College of BioNano Technology, Gachon University)
Publication Information
Korean Journal of Food Science and Technology / v.50, no.6, 2018 , pp. 594-600 More about this Journal
Abstract
Shiga toxin-producing Escherichia coli (STEC) is an important pathogenic bacterium. To control STEC, the characteristics of the ECP33 and NOECP91 coliphages, which belong to the Myoviridae family, were analyzed. The host inhibition range for a total of 44 STEC strains was 45.5% for ECP33 and 65.9% for NOECP91. ECP33 and NOECP91 were relatively stable at $65^{\circ}C$, 50 ppm of sodium hyperchlorite, and a pH value of 4-10. However, the two phages were susceptible to a temperature of $70^{\circ}C$. NOECP91 was killed within 1 h after exposure to 30% ethanol, but ECP33 showed high tolerance even after exposure to 70% ethanol for 1 h. Interestingly, the inhibition of STEC growth according to the multiplicity of infection of 0.1 was confirmed until no growth was observed after 10 hours of culture with the phages. Therefore, the ECP33 and NOECP91 phages may be applied as a biological control agent for Shiga toxin-producing E. coli.
Keywords
shiga toxin-producing E. coli; coliphage; host spectrum; stability; biocontrol;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Ackermann HW. Bacteriophage observations and evolution. Res. Microbiol. 154: 245-251 (2003)   DOI
2 Brooks JT, Sowers EG, Wells JG, Greene KD, Griffin PM, Hoekstra RM, Strockbine NA. Non-O157 shiga toxin-producing Escherichia coli infections in the United States, 1983-2002. J. Infect. Dis. 192: 1422-1429 (2005)   DOI
3 Brussow H. Phage therapy: The Escherichia coli experience. Microbiol. 151: 2133-2140 (2005)   DOI
4 Buzrul S, Ozturk P, Alpas H, Akcelik M. Thermal and chemical inactivation of lactococcal bacteriophages. LWT-Food Sci. Technol. 40: 1671-1677 (2007)   DOI
5 Dini C, Urraza PJ. Isolation and selection of coliphages as potential biocontrol agents of enterohemorrhagic and shiga toxin-producing E. coli (EHEC and ETEC) in cattle. J. Appl. Microbiol. 109: 873-887 (2010)   DOI
6 Frank C, Werber D, Cramer JP, Askar M, Faber M, Heiden M, Bernard H, Fruth A, Prager R, Spode A, Wadl M. Epidemic profile of shiga-toxin-producing Escherichia coli O104: H4 outbreak in Germany. New Eng. J. Med. 365: 1771-1780 (2011)   DOI
7 Gould LH, Mody RK, Ong KL, Clogher P, Cronquist AB, Garman KN, Lathrop S, Medus C, Spina NL, Webb TH, White PL. Increased recognition of non-O157 shiga toxin-producing Escherichia coli infections in the United States during 2000-2010: Epidemiologic features and comparison with E. coli O157 infections. Foodborne Pathog. Dis. 10: 453-460 (2013)   DOI
8 Guglielmotti DM, Mercanti DJ, Reinheimer JA, Quiberoni Adel L. Efficiency of physical and chemical treatments on the inactivation of dairy bacteriophages. Front. Microbiol. 2: 282 (2011)
9 Hendrix RW. Bacteriophage genomics. Curr. Opin. Microbiol. 6: 506-511 (2003)   DOI
10 Joczyk E, Kak M, Mizybrodzki R, Gorski A. The influence of external factors on bacteriophages. Folia Microbiol. 56: 191-200 (2011)   DOI
11 Kaper JB, Nataro JP, Mobley HL. Pathogenic Escherichia coli. Nat. Rev. Microbiol 2: 123-140 (2004)   DOI
12 Karch H, Denamur E, Dobrindt U, Finlay BB, Hengge R, Johannes L, Ron EZ, Tonjum T, Sansonetti PJ, Vicente M. The enemy within us: Lessons from the 2011 European Escherichia coli O104: H4 outbreak. EMBO Mol. Med. 4: 841-848 (2012)   DOI
13 Khakhria R, Duck D, Lior H. Extended phage-typing scheme for Escherichia coli 0157: H7. Epidemiol. Infect. 105: 511-520 (1990)   DOI
14 Kim JY. Characterization of bacteriophage for enteroheamorrhagic E. coli and application as sanitizer. MS thesis, Gachon University, Sungnam, Korea (2013)
15 Kim EJ, Chang HJ, Kwak SJ, Park JH. Virulence factors and stability of coliphages specific to Escherichia coli O157:H7 and to various E. coli infection. J. Microbiol. Biotechnol. 26: 2060-2065 (2016a)   DOI
16 Kim MJ, Kim SH, Kim TS, Kee HY, Seo JJ, Kim ES, Park JT, Chung JK, Lee J. Identification of shiga toxin-producing E. coli isolated from diarrhea patients and cattle in Gwangju area, Korea. J. Bacteriol. Virol. 39: 29-39 (2009)   DOI
17 Kim EJ, Lee H, Lee JH, Ryu S, Park JH. Morphological features and lipopolysaccharide attachment of coliphages specific to Escherichia coli O157: H7 and to a broad range of E. coli hosts. Appl. Biol. Chem. 59: 109-116 (2016b)   DOI
18 Law D. Virulence factors of Escherichia coli O157 and other shiga toxin-producing E. coli. J. Appl. Microbiol. 88: 729-745 (2000)   DOI
19 Lee YD, Kim JY, Park JH. Characteristics of coliphage ECP4 and potential use as a sanitizing agent for biocontrol of Escherichia coli O157:H7. Food Control 34: 255-260 (2013)   DOI
20 Lee YD, Park JH. Isolation and characterization of temperate phages in Enterococcus faecium from Sprouts. Korean J. Food Sci. Technol. 46: 323-327 (2014)   DOI
21 Lee YD, Park JH. Characterization and application of phages isolated from sewage for reduction of Escherichia coli O157:H7 in biofilm. LWT-Food Sci. Technol. 60: 571-577 (2015)   DOI
22 Mathusa EC, Chen Y, Enache E, Hontz L. Non-O157 shiga toxinproducing Escherichia coli in foods. J. Food Protect. 73: 1721-1736 (2010)   DOI
23 Lienemann T, Kyyhkynen A, Halkilahti J, Haukka K, Siitonen A. Characterization of Salmonella Typhimurium isolates from domestically acquired infections in Finland by phage typing, antimicrobial susceptibility testing, PFGE and MLVA. BMC Microbiol. 15: 131 (2015)   DOI
24 Majowicz SE, Scallan E, Jones-Bitton A, Sargeant JM, Stapleton J, Angulo FJ, Yeung DH, Kirk MD. Global incidence of human shiga toxin-producing Escherichia coli infections and deaths: A systematic review and knowledge synthesis. Foodborne Pathog. Dis. 11: 447-455 (2014)   DOI
25 Marks T, Sharp R. Bacteriophages and biotechnology: A review. J. Chem. Tech. Biot. 75: 6-17 (2000)   DOI
26 Minakhin L, Goel M, Berdygulova Z, Ramanculov E, Florens L, Glazko G, Karamychev VN, Slesarev AI, Kozyavkin SA, Khromov I, Ackermann HW. Genome comparison and proteomic characterization of Thermus thermophilus bacteriophages P23-45 and P74-26: Siphoviruses with triplex-forming sequences and the longest known tails. J. Mol. Biol. 378: 468-480 (2008)   DOI
27 Nataro JP, Kaper JB. Diarrheagenic Escherichia coli. Clin. Microbiol. Rev. 11: 142-201 (1998)   DOI
28 Oliveira A, Sillankorva S, Quinta R, Henriques A, Sereno R, Azeredo J. Isolation and characterization of bacteriophages for avian pathogenic E. coli strains. J. Appl. Microbiol. 106: 1919-1927 (2009)   DOI
29 Nakao H, Kataoka C, Kiyokawa N, Fujimoto J, Yamasaki S, Takeda T. Monoclonal antibody to shiga toxin 1, which blocks receptor binding and neutralizes cytotoxicity. Microbiol. Immunol. 46: 777-780 (2002)   DOI
30 O'Flynn G, Ross RP, Fitzgerald GF, Coffey A. Evaluation of a cocktail of three bacteriophages for biocontrol of Escherichia coli O157:H7. Appl. Environ. Microbiol. 70: 3417-3424 (2004)   DOI
31 Sulakvelidze A, Alavidze Z, Morris JG. Bacteriophage therapy. Antimicrob. Agents Ch. 45: 649-659 (2001)   DOI
32 Raya RR, Varey P, Oot RA, Dyen MR, Callaway TR, Edrington TS, Kutter EM, Brabban AD. Isolation and characterization of a new T-even bacteriophage, CEV1, and determination of its potential to reduce Escherichia coli O157: H7 levels in sheep. Appl. Environ. Microbiol. 72: 6405-6410 (2006)   DOI
33 Sanekata T, Fukuda T, Miura T, Morino H, Lee C, Maeda K, Araki K, Otake T, Kawahata T, Shibata T. Evaluation of the antiviral activity of chlorine dioxide and sodium hypochlorite against feline calicivirus, human influenza virus, measles virus, canine distemper virus, human herpesvirus, human adenovirus, canine adenovirus and canine parvovirus. Biocontrol Sci. 15: 45-49 (2010)   DOI
34 Santos TMA, Gilbert RO, Caixeta LS, Machado VS, Teixeira LM, Bicalho RC. Susceptibility of Escherichia coli isolated from uteri of postpartum dairy cows to antibiotic and environmental bacteriophages. Part II: In vitro antimicrobial activity evaluation of a bacteriophage cocktail and several antibiotics. J. Dairy Sci. 93: 105-114 (2010)   DOI
35 Sass P, Bierbaum G. Lytic activity of recombinant bacteriophage 11 and 12 endolysins on whole cells and biofilms of Staphylococcus aureus. Appl. Environ. Microbiol. 73: 347-352 (2007)   DOI
36 Smith JL, Fratamico PM, Gunther IV NW. Shiga toxin-producing Escherichia coli. Adv. Appl. Microbiol. 86: 145-197 (2014)
37 Tzipilevich E, Habusha M, Ben-Yehuda S. Acquisition of phage sensitivity by bacteria through exchange of phage receptors. Cell 168: 186-199 (2017)   DOI