• Microbiology and Biotechnology Letters
• /
• v.38 no.2
• /
• pp.144-150
• /
• 2010

This study was aimed to screen bacteria of high alginate-degrading activity, to select the nitrogen source and concentration of NaCl and sodium alginate for the production of alginate-degrading enzyme, and to determine reaction conditions of enzyme. A novel alginate-degrading bacterium was isolated from abalone (Haliotis discus hannai) and named Methylobacterium sp. HJM27 by 16S rDNA sequence analysis. The optimum culture conditions for the production of alginate-degrading enzyme were 1.0% sodium alginate, 0.5% peptone, 0.3% yeast extract, 1.5% NaCl, $25^{\circ}C$ and 48 hours incubation time. The raw enzyme showed the highest activity at $25^{\circ}C$ and pH 9, and produced 1.217 g - reducing sugar per liter in 0.8% (w/v) sodium alginate for 30 minutes. Methylobacterium sp. HJM27 and its alginate-degrading enzyme would be useful for the production of bioenergy and biofunctional oligosaccharides from seaweed.

• Microbiology and Biotechnology Letters
• /
• v.42 no.2
• /
• pp.190-193
• /
• 2014

CMP-Neu5Ac synthetase is a key enzyme for the synthesis of CMP-Neu5Ac, which is an essential precursor of sialylated glycoconjugates. For the soluble expression of the CMP-Neu5Ac synthetase gene (neuA) from Escherichia coli K1, various heat shock proteins were co-expressed in E. coli BL21 (DE3) Star. In order to do this, a pG-KJE8 plasmid, encoding genes for GroEL-ES and DnaK-DnaJ-GrpE, was co-transformed with neuA and was expressed at $20^{\circ}C$ by the addition of 0.01 mM IPTG and 0.005 mg/ml L-arabinose. The co-expression of a variety of heat shock proteins resulted in the remarkably improved production of soluble CMP-Neu5Ac synthetase in E. coli.

• Journal of Korean Medical Ki-Gong Academy
• /
• v.16 no.1
• /
• pp.1-58
• /
• 2016

Objects : The purpose of this study is to understand trends of "Qigong"-related study since 2008 and to help guide the research direction of Qigong study. Methods : The computerized Korean database was searched from January 2008 until September 2016. The search terms used were 'Qi', 'Qigong', 'Doin', 'Meditation', 'bioenergy', 'training', 'life nurturing' and random or Korean language terms. Results : 1. A total of 140,914 studies were searched in this study. A total of 2,147 studies were finally selected as Qigong-related studies. 2. The average number of Qigong-related studies published in Korea since 2008 is 252.6 per year and there was no significant difference in the number of studies published by year 3. Results according to the subject categories, 805 cases were in the humanities and 2 cases were in the agriculture & maritime field. 4. Results according to the middle subject categories, philosophy was the largest with 280 studies. 5. As a result of Qigong categories showed that meditation was the highest with 1,043 (48.58%) not including duplication. 6. As a result of research method, the most frequent method was Analysis research with 1,138(53.00%) cases and the experimental research was the least with 118(5.50%) cases. 7. When the authors were investigated, the result was the most in 35 cases by Kim Byung-chae. 8. The journal that published the most Qigong-related studies was "J. of The Studies of Taoism and Culture" (52), and Dongguk University (75) had the largest number of Qigong-related studies. Conclusions : 1. Since 2008, there is no significant difference between the yearly and yearly the number of Qigong-related studies. 2. Since 2008, Qigong-related studies have been the most successful in the field of humanities, but it has been regularly published in various other field. 3. Since 2008, Qigong-related studies has shown a remarkable decline in category on External Qigong Therapy(外氣發功) and Science of Qi(氣科學). However, category on Nae-Dan(內丹), meditation, Do-In (導引) has continued steadily every year. And did not show a tendency to increase or decrease.

• Korean Journal of Soil Science and Fertilizer
• /
• v.31 no.4
• /
• pp.384-391
• /
• 1998

Nutrient removal during stem harvest was evaluated in a one-year rotation willow bioenergy plantation. For the stem nutrient content, the stem biomass and stem nutrient concentration were collected in the winter of 1987-1993 from the established willow plantation at Tully, New York, U.S.A. in 1987. Five willow clones and one hybrid poplar clone were planted. Half of the plots were fertilized annually with $336kg\;ha^{-1}$ N, $112kg\;ha^{-1}$ P, and $224kg\;ha^{-1}$ K. All trees were harvested annually. Mean annual nutrient removals of N, P, K, Ca, and Mg by annual stem harvesting over seven years were respectively 30-70, 4-10, 14-40, 19-59 and $3-5kg\;ha^{-1}$. Fertilized plants exported higher quantities of nutrients than non-fertilized ones. Nontheless, quantities of nutrients exported were well below the quantities supplied by fertilization suggesting that nutrients removal by stem harvesting is not likely to cause a decrease in soil fertility. However, in non-fertilized plots, the amount of nutrients removed could result in decrease of nutrient availability and soil fertility over the long-term. An evaluation of the clones revealed that clone SV1 is the most nutrient efficient.

• Korean Journal of Food Science and Technology
• /
• v.50 no.3
• /
• pp.308-315
• /
• 2018

To select suitable cultivars to produce sweet potato (SP) chips instead of potato chips, ten Korean cultivars, Sinjami, Yeunjami, Sinhwangmi, Juhwangmi, Sincheonmi, Sinyulmi, Gunhwangmi, Dahomi, Daeyumi, and Pungwonmi were used. The acrylamide content, quality characteristics, and oxidation stability of SP chips were investigated. Acrylamide content was shown to be under 0.48 mg/kg in case of all cultivars except Yeunjami (1.07 mg/kg). The color values significantly differed among the cultivars and were maintained like those of raw roots. The hardness, brittleness, and fracturability of Juhwangmi and Sinhwangmi chips showed the lowest values, while those of Daeyumi were the highest. According to the sensory evaluation data, the quality characteristics of chips were influenced by not only hardness, fracturability, and crunchiness, but also the flavor taste. Pungwonmi chips showed the highest score, followed by Gunhwangmi and Dahomi chips. Therefore, Pungwonmi, Dahomi, and Gunhwangmi are suggested as suitable cultivars to make SP chips, because of their high overall quality and very low acrylamide content.

• Ecology and Resilient Infrastructure
• /
• v.6 no.3
• /
• pp.163-170
• /
• 2019

The civil engineering materials used to stabilize the slopes of new riverbanks have a great impact on the types and growth of vegetation introduced after the completion of construction procedure. Recently, microbial-derived, ${\beta}$-glucan- and xanthan gum-based biopolymers are attracting attention as an ecofriendly strengthening material of riverbanks that can possibly stimulate plant growth. This study aimed to assess ecological effects of biopolymer application on native plants in Korean riverbanks. In particular, since dominant plant species could shape characteristics of an ecosystem, we examined the effects of biopolymer on the dominant plant species in riverbanks. Overall, biopolymer did not affect seed germination rates of testing plant species. In contrast, plants grew more vigorously in the soil mixed with biopolymer compared to those in the control soil. The biomass of Echinochloa crus-galli especially increased around two times more in the biopolymer treatment. Plants produced heavier root biomass and leaves with larger specific leaf area, which possibly contributes to the tolerance of environmental stress like drought. These results suggest that biopolymers treated on river banks are expected to stimulate plant growth and increase stress tolerance of domestic dominant plant species.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
• /
• v.14 no.2
• /
• pp.168-174
• /
• 2021

According to the World Agricultural Productivity Report, the current annual average growth rate of agriculture is 1.63%, which is lower than 1.73% to support the world's 10 billion people, which is growing by 2050. The demand for food, feed, and bioenergy is not growing enough to continue to meet the demand, and it is predicting a future food shortage. The purpose of this study was to create a regional irrigation control model for the purpose of reducing the production cost of crops, increasing production, and improving quality, and presenting a model that can give advice to farmers who start farming in the region. The irrigation control modeling presented in this study means to represent the change of medium weight·supply liquid·drainage amount due to changes in the root zone environment according to the passage of time and climate in a graph model. For water control modeling, we collected data on the change in the amount of the root zone environment and the weight of the badge·supply amount·drainage amount from March to June in Nonsan, Buyeo, and Yesan regions in Chungnam Province through the measuring device of the Ministry of Environment in the root region. We set up the parameters for derivation and derived an irrigation control model that can confirm the change in weight·supply liquid·drainage amount over time through the parameters.

• Journal of Climate Change Research
• /
• v.9 no.4
• /
• pp.303-312
• /
• 2018

In Ethiopia, climate change and deforestation are major issues hindering sustainable development. Local Ethiopian communities commonly perceive an increase in temperature and a decrease in rainfall. Meteorological data shows that rainfall has declined in southern Ethiopia, and spring droughts have occurred more frequently during the last 10-15 years. The frequently occurring droughts have seriously affected the agriculture-dominated Ethiopian economy. Forests can play an important role in coping with climate change. However, deforestation is alarmingly high in Ethiopia, and this is attributed mainly to agricultural expansion and fuel wood extraction. Deforestation has led to a decrease in various benefits from forest ecosystem services, and increased ecological and environmental problems including loss of biodiversity. To resolve the issues effectively, it is crucial to enhance climate change resilience through reforestation and various international collaborations are urgently needed. To continue collaboration activities for resolving these issues, it is first necessary to address fundamental questions on the nature of collaboration: does collaboration aim for a support-benefit or a mutual benefit situation; dividing the workload or sharing the workload; an advanced technology or an appropriate technology; and short-term and intensive or long-term and extensive?. Potential collaboration activities were identified by sectors: in the governmental sector, advancing governmental structure and policy, enhancing international collaborations and negotiations, and capacity building for forest restoration and management; in the research and education sector, identifying and filling gaps in forestry and climate change education, capacity building for reforestation and climate change resilience research, and developing bioenergy and feed stocks; and in the business and industry sector, supporting conservation based forestry businesses and industries, while promoting collaboration with the research and education sectors. It is envisaged that international collaboration for enhancing climate change resilience through reforestation will provide a strong platform for resolving climate change and deforestation issues, and achieving sustainable development in Ethiopia.

• Korean Journal of Breeding Science
• /
• v.41 no.4
• /
• pp.463-467
• /
• 2009

Interspecific hybrid plants between Allium cepa L. (2n=2X=16) and A. fistulosum L. (2n=2X=16)and their backcross lines were developed by artificial pollination in order to introduce new desirable characters of A, cepa to A. fistulosum. The 2C nuclear DNA content has been estimated by flow cytometry in 5 Allium fistulosum inbreed lines, 2 interspecific hybrid lines of A. cepa${\times}$A. fistulosum and 34 their backcross lines $BC_1F_1$ to $BC_2F_2$, using propidium iodide (PI) as a fluorescence dye. Estimated 2C DNA values ranged from 22.2 pg to 23.7 pg in 5 A. fistulosum inbreed lines, 37.9 pg in F1 hybrid between A. cepa and A. fistulosum, 24.3 pg to 27.3 pg in 7 backcross lines in $BC_1F_1$, 21.9 pg to 24.4 pg in 9 $BC_1F_2$, 22.9 pg to 25.1 pg in 14 $BC_2F_1$, 22.6 pg to 23.4 pg in 4 $BC_2F_2$. This study showed mean 2C nuclear DNA content of $F_1$ hybrid was higher than their backcross progeny lines, while it was lower than female parental line, A. cepa (2C DNA=33.2 pg). Mean 2C DNA content of backcross lines, $BC_1F_1$ to $BC_2F_2$ was not significantly different but their 2C DNA contents in the more progress generation from $BC_1F_1$ to $BC_2F_2$ were reduced.

• Korean Journal of Breeding Science
• /
• v.41 no.4
• /
• pp.468-473
• /
• 2009

Fluorescence in situ hybridization (FISH) and genomic in situ hybridization (GISH) were used for chromosome analysis of hybrids (2n=16) between onion (Allium cepa L., 2n=2X=16) and welsh onion (A. fistulosum L., 2n=2X=16). 5S rDNA, 45S rDNA, and tandemly repeated DNA (TSD) sequence were used as probes for FISH analysis. A. fistulosum specific DNA probe of telomeric repeats and A. fistulosum DNA were used for GISH analysis. In the analysis of meiotic chromosome GISH revealed that hybrids have 7 bivalants and 2 univalents chromosome and 2 univalents were derived from A. fistulosum chromosomes. In somatic chromosomes of hybrid each 8 chromosomes were derived from A. cepa and A. fistulosum, respectively. FISH signal of 45S rDNA probe in A. fistulosum was detected at secondary constriction of chromosomes, while FISH signal in A. cepa was observed in both secondary constriction and telomere of chromosomes. TDS signals in A. fistulosum chromosomes were detected at all subtelomeric of 8 chromosomes and also in 2 pericentromeric of the chromosomes, whereas TDS signals in A. cepa were observed only in subtelomeric in all chromosomes. The pattern of TDS signal in hybrid chromosomes was similar to those of A. fistulosum chromosomes.