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http://dx.doi.org/10.4014/kjmb.1403.03008

Soluble Production of CMP-Neu5Ac Synthetase by Co-expression of Chaperone Proteins in Escherichia coli  

Choi, Hwa Young (Department of Food Science and Technology, BK21 Education and Research Center for Advanced Bio-Agriculture Technology, Chungbuk National University)
Li, Ling (Department of Food Science and Technology, BK21 Education and Research Center for Advanced Bio-Agriculture Technology, Chungbuk National University)
Cho, Seung Kee (Department of Food Science and Technology, BK21 Education and Research Center for Advanced Bio-Agriculture Technology, Chungbuk National University)
Lee, Won-Heong (Department of Bioenergy Science and Technology, Chonman National University)
Seo, Jin-Ho (Department of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University)
Han, Nam Soo (Department of Food Science and Technology, BK21 Education and Research Center for Advanced Bio-Agriculture Technology, Chungbuk National University)
Publication Information
Microbiology and Biotechnology Letters / v.42, no.2, 2014 , pp. 190-193 More about this Journal
Abstract
CMP-Neu5Ac synthetase is a key enzyme for the synthesis of CMP-Neu5Ac, which is an essential precursor of sialylated glycoconjugates. For the soluble expression of the CMP-Neu5Ac synthetase gene (neuA) from Escherichia coli K1, various heat shock proteins were co-expressed in E. coli BL21 (DE3) Star. In order to do this, a pG-KJE8 plasmid, encoding genes for GroEL-ES and DnaK-DnaJ-GrpE, was co-transformed with neuA and was expressed at $20^{\circ}C$ by the addition of 0.01 mM IPTG and 0.005 mg/ml L-arabinose. The co-expression of a variety of heat shock proteins resulted in the remarkably improved production of soluble CMP-Neu5Ac synthetase in E. coli.
Keywords
CMP-Neu5Ac; heterologous protein; heat shock proteins; chaperone; recombinant E. coli;
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