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Isolation and Characteristics of Alginate-Degrading Methylobacterium sp. HJM27  

Kim, Ok-Ju (Department of Bioscience and Biotechnology, Graduate School, Silla University)
Lee, Dong-Geun (Department of Bioscience and Biotechnology, Graduate School, Silla University)
Lee, Sung-Mok (Department of Bioscience and Biotechnology, Graduate School, Silla University)
Lee, Suck-June (Department of Bioscience and Biotechnology, Graduate School, Silla University)
Do, Hyung-Joo (Department of Bioscience and Biotechnology, Graduate School, Silla University)
Park, Hye-Jin (Department of Pharmaceutical Engineering, College of Medical & Life Science, Silla University)
Kim, Andre (Department of Pharmaceutical Engineering, College of Medical & Life Science, Silla University)
Lee, Jae-Hwa (Department of Bioscience and Biotechnology, Graduate School, Silla University)
Ha, Jong-Myung (Department of Bioscience and Biotechnology, Graduate School, Silla University)
Publication Information
Microbiology and Biotechnology Letters / v.38, no.2, 2010 , pp. 144-150 More about this Journal
Abstract
This study was aimed to screen bacteria of high alginate-degrading activity, to select the nitrogen source and concentration of NaCl and sodium alginate for the production of alginate-degrading enzyme, and to determine reaction conditions of enzyme. A novel alginate-degrading bacterium was isolated from abalone (Haliotis discus hannai) and named Methylobacterium sp. HJM27 by 16S rDNA sequence analysis. The optimum culture conditions for the production of alginate-degrading enzyme were 1.0% sodium alginate, 0.5% peptone, 0.3% yeast extract, 1.5% NaCl, $25^{\circ}C$ and 48 hours incubation time. The raw enzyme showed the highest activity at $25^{\circ}C$ and pH 9, and produced 1.217 g - reducing sugar per liter in 0.8% (w/v) sodium alginate for 30 minutes. Methylobacterium sp. HJM27 and its alginate-degrading enzyme would be useful for the production of bioenergy and biofunctional oligosaccharides from seaweed.
Keywords
Alginate; alginate-degrading enzyme; brown algae; Methylobacterium sp.; marine organism; seaweed;
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