• 한국자원식물학회지
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• 제28권1호
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• pp.93-100
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• 2015

유채 종자에서 기름을 착유한 후 부산물인 유채박을 토양에 처리하여 토양 내 선충을 친환경적으로 방제하기 위하여 실험한 결과를 보면, 유채박을 혼합한 토양이 OM, $Av.P_2O_5$, Ca, Mg, CEC 등의 수치가 증가하였으며, 유기물함량은 무처리 토양보다 3배가량 높게 나타났다. 또한 유채박의 glucosinolate 함량은 제주재래종 유채박이 $199.97{\mu}mol/g$로 선망 유채박의 함량보다 $76.62{\mu}mol/g$ 더 많았으며, 특히 progoitrin, gluconapin, glucobrassicanapin, 및 sinigrin 등의 함량이 높게 나타나기 때문에 유채박의 선충밀도 감소에 효과가 있으며 '선망' 유채박보다 제주재래종 유채박이 선충밀도를 더 줄여주었지만 유의적인 차이는 아니었다.

• 한국해양바이오학회지
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• 제8권2호
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• pp.45-53
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• 2016

Most of microalgae shift their metabolic pathways toward the fatty acid biosynthesis following nitrogen deprivation. Recent studies on Nannochloropsis species, oleaginous microalgae, have been performed to investigate the regulation of contents and compositions of fatty acids under stressful condition. The objective of this experiment is to identify the effect of nitrogen on cell growth and fatty acids production in Nannochloropsis oculata K-1281 and compare fatty acid composition response to nitrogen deficiency between N. oculata LB2164 and K-1281. The fatty acids content in N. oculata K-1281 was increased up to 210%, while the growth rate was decreased under nitrogen deficient condition. The contents of C16:0 and C16:1 increased dramatically in both N. oculata K-1281 and LB2164, while the contents of C20:4 and C20:5 increased in N. oculata LB2164. The fatty acids content and composition in N. oculata K-1281 returned following addition of nitrogen after nitrogen starvation. These results demonstrated that fatty acid contents and compositions under nitrogen deficiency will provide the understanding of fatty acid synthesis in microalgae.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.323-323
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• 2022

Jack bean [Canavalia ensiformis (L.)], belonging to the Leguminosae family has been frequently used in edible and medicinal plants in Asian countries. Jack beans are high in protein which is approximately 30%. Concanavalin A (Con A) is a major protein of Jack bean and belongs to the family of legume lectins. It has inhibitory effect on hepatocellular carcinoma by inducing autophagy. However, Con A negatively affects nutrient utilization by other mechanisms. It binds to the glycoproteins and glycolipids of the digestive tract mucosa, inhibits the activity of the enzymes of the brush border of the enterocytes. In order to use Jack bean young seedpods, they are restricted to 'young pods (soft, pre-swelling)' according to the 'Food Code' (Ministry of Food and Drug Safety). Therefore, in this study, we investigated the quantitative change of Con A across developmental stages of Jack bean pods. Biological samples consisted of Jack bean pods and seeds in 7 stages of development. The expression pattern of Con A mRNA was monitored by quantitative reverse transcription PCR (RT-qPCR). Expression of Con A proteins was analyzed by western blotting. The expression of Con A mRNA and protein in the seeds tended to increase gradually as the seeds expanded. However, in pods, they were much less than in seeds. As the expression of Con A mRNA and protein increases as the pods thicken, it is predicted that Con A synthesis increases when the thickness growth of the pod begins after the length growth of the pod is completed. Since the expression of Con A in the pods and seeds in very low when the pods are about 2 cm, therefore 2 cm pods seem appropriate when using 'young pods'. It is also necessary to study other proteins in Jack bean, such as Urease and Canavalin. These studies will serve as the basis for processing Jack bean.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.229-229
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• 2022

Rapeseed is a crop that is waterlogging sensitive, and it is necessary to breed waterlogging tolerance varieties. Our study presents the comparative transcriptome changes in two rapeseed lines, i.e., waterlogging-tolerant (tJ8634-B-30,) and - sensitive ('EMS26') lines under control and waterlogging stress treatments at the flowering stage. RNA-sequencing analysis revealed 13,279 differentially expressed genes (DEGs) for 'J8634-B-30' and 8,682 DEGs for 'EMS26' under waterlogging stress condition compared to control. Among DEGs of 'J8634-B-30', 6,818 were up-regulated and 6,461 were down-regulated. On the other hand, among the DEGs of 'EMS26', the number of down-regulated genes (5,240) were higher than that of up-regulated genes (3,442). Gene ontology enrichment analysis showed that DEGs related to glucan metabolic, cell wall, and oxidoreductase activity were significantly changed in 'J8634-B-30'. Kyoto Encyclopedia of Genes and Genomes (KEGG)-based analysis in 'J8634-B-30' identified up-regulated DEGs being involved in MAPK signaling pathways. In addition, the DEGs belonging to mechanisms responding to waterlogging stress, i.e., plant hormones, carbon metabolism, Reactive oxygen species (ROS), Nitric oxide (NO) etc. were compared in rapeseed lines. Several DEGs including ethylene-responsive transcription factor (ERF), constitutive triple response (CTR) (in ethylene signaling pathway), monodehydroascorbate Reductase (MDAR), NADPH oxidase (in ROS pathway), cytochrome c oxidase assembly protein (COX) (in NO pathway) up-regulated in 'J8634-B-30'. These outcomes provided the valuable information for further exploring the genetic mechanism of waterlogging tolerance in rapeseed.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.192-192
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• 2022

Sweetpotato is rich in starch, which is converted to sugar during storage due to enzymatic hydrolysis. The sugar content of sweetpotato is a component related to taste and storability. In this study, the sugar content (fructose, glucose, maltose, sucrose and total sugar content) of 94 genotypes was evaluated and the GWAS (Genome-Wide Association Study) was conducted to search for candidate genes for sugar content. The fructose and glucose content were 0.2 ~ 8.8 and 0.2 ~ 9.4 g/100g, respectively. The maltose, sucrose and total sugar content were 0.2 ~ 9.1,3.2 - 30.0 and 7.9 ~ 40.2 g/100g, respectively. The fructose and glucose showed a positive correlation (0.98). The 94 genotypes were genotyped with genotyping-by-sequencing (GBS) and aligned against the reference genome sequences of sweetpotato. The GBS libraries from 94 genotypes were sequenced on an Illumina HiSeqXten system, and 1,339,892 SNPs (Single Nucleotide Polymorphism) were generated. Filtering for < 60% missing rate and > 0.05 minor allele frequency resulted in a total of 44,255 SNPs used in GWAS. The GAPIT (Genome Association and Prediction Integrated Tool) was used to conduct based on the mean of sugar content with a Bonferroni-corrected chromosome-wide significance threshold with a -logio(P) of 5.95. The significant SNPs were obtained with fructose (seven), glucose (six), maltose (four) and sucrose (nine). There were several genes related to sugar content around the significant SNPs such as sugar transport protein 8-like, probable galactose-1 -phosphate uridyltransferase-like and beta-amylase. These results will contribute to understanding of sugar content and conversion in sweetpotato.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2022년도 추계학술대회
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• pp.83-83
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• 2022

유채(Brassica napus L.)는 가을에 파종하여 이듬해에 초여름에 수확하는 겨울작물로 종실 수량이 많고 종자의 조지방 함량이 높아 주로 기름을 생산하기 위해서 재배되고 있다. 우리나라에서 유채는 경관을 목적으로 주로 재배되며 면적은 약 5,000ha 정도 재배되고 있다. 최근에는 유채유 생산을 목적으로 전남 등 남부지방에서 재배면적이 증가하고 있다. 유채유의 대량 생산을 위해서는 재배과정의 생력기계화에 유리한 논 재배가 주로 이루어지고 있다. 따라서 유채의 육종은 논 재배 적응성이 뛰어나며, 벼와의 작부가 가능하며 봄 파종 재배가 가능한 조숙품종의 육성이 필요하며, 식용유로 이용이 가능한 지방산 조성이 우수한 품종의 육성도 필요하다. 본 연구는 농촌진흥청 농업유전자원센터에서 보유하고 있는 유채 유전자원 350점을 대상으로 작물학적 생육특성을 평가하였고 종자를 수확한 후 지방산 조성을 분석하였다. 생육특성은 경장 등 12항목을 유채 유전자원 특성조사 및 관리요령(RDA, 2011)을 기준으로 조사하였다. 가을에 파종하여 재배할 때 개화소요일수는 파종 후 137일부터 210일까지 소요되었으며, 봄 파종 재배 시에는 파종 후 65일부터 150일까지 개화가 진행되었고 개화가 되지 않은 계통이 67계통이었다. 경장은 85 ~ 211cm, 수장은 28 ~ 79cm, 분지수는 5 ~ 21개, 수당 협수는 29 ~ 106개, 협당 종자수는 18 ~ 35개 및 협장은 2.7 ~ 8.8cm로 다양하였다. 유채 유전자원의 지방산 중 올레산과 에루스산 함량은 각각 9.7 ~ 70.4% 및 0 ~ 54.7% 범위였다. 공시계료 중 IT 279089 등 3자원은 개화기가 빨라 조생종 육성에, IT279125 등 3자원은 올레산 함량이 68%이상으로 양질 지방산 품종 육성재료로 활용할 예정이다.

• Journal of Microbiology and Biotechnology
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• 제28권3호
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• pp.448-453
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• 2018

In this study, a 107 kDa protease from psychrophilic Janthinobacterium lividum PAMC 26541 was purified by anion-exchange chromatography. The specific activity of the purified protease was 264 U/mg, and the overall yield was 12.5%. The J. lividum PAMC 25641 protease showed optimal activity at pH 7.0-7.5 and $40^{\circ}C$. Protease activity was inhibited by PMSF, but not by DTT. On the basis of the N-terminal sequence of the purified protease, the gene encoding the cold-adapted protease from J. lividum PAMC 25641 was cloned into the pET-28a(+) vector and heterologously expressed in Escherichia coli BL21(DE3) as an intracellular soluble protein.

• 한국태양에너지학회:학술대회논문집
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• 한국태양에너지학회 2008년도 추계학술발표대회 논문집
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• pp.132-140
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• 2008

Recently, the whole world is concerned about the saving energy and protective environment, so interest is increasing in new and renewable energy. Specially the Bioenergy continuity is possible, the research is advanced by the energy which it contributes in environmental conservation. From the research which it sees consequently it investigates about co-generation system of domestic bio-energy, it is used to analyze the electricity and heat energy of buildings that Energy Consumption Survey of Korea Energy Management Corporation and food waste generation quantity of Ministry of Environment. This paper is analyzed that application of food waste Biogas plant system.

• 폴리머
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• 제38권4호
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• pp.464-470
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• 2014

A chemo-mechanical method was used to prepare lignin-containing cellulose nanofibrils(L-CNF) from unbleached woodpulps dispersed uniformly in an organic solvent. L-CNF/PLA composites were obtained by solvent casting method. The effects of L-CNF concentration on the composite performances were characterized by tensile test machine, contact angle machine, scanning electron microscope (SEM), and Fourier transform infrared spectroscopy (FTIR). The tensile test results indicated that the tensile strength and elongation-at-break increased by 50.6% and 31.8% compared with pure PLA. The contact angle of PLA composites decreased from $79.3^{\circ}$ to $68.9^{\circ}$. The FTIR analysis successfully showed that L-CNF had formed intermolecular hydrogen bonding with PLA matrix.

• The Plant Pathology Journal
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• 제27권2호
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• pp.164-169
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• 2011

Previous studies have addressed the management of phyllosphere pathogens by leaf and root-associated microbes. The present study evaluated the effect of the foliar application of three strains of Bacillus spp. on plant growth and fruit quality. The application of a bacilli spore preparation significantly improved leaf growth parameters such as leaf thickness and photosynthesis capacity, indicating that bacilli treatment directly promoted leaf growth. In addition, foliar treatment resulted in an improvement in the key indicators of fruit quality including water, glucose, and sucrose contents. The present results suggest that foliar spraying of beneficial bacilli is a potential treatment of wide application for the improvement of apple quality. Foliar application of bacilli preparation as effective plant growth-promoting rhizobacteria broadens the spectrum of their availability for orchard application.