• Title/Summary/Keyword: biochemical research

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Biochemical, Haematological and Thyroid Hormone Profile in Healthy Indian Kathiawari Horses

  • Gupta, A.K.;Kumar, Sanjay;Pal, Yash
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.8
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    • pp.1215-1221
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    • 2002
  • Normal haematological and biochemical indices along with thyroid hormone status were studied in healthy Kathiawari horses of different age groups (yearling, young stock, adults and old stock) belonging to either sex. Effect of both age and sex was observed on thyroid hormone levels, haematological and biochemical indices. In females, hemoglobin levels was significantly lower in yearlings than adult animals while total leukocyte counts were higher in yearlings than equids of other age groups. Sex had effect only on total erythrocyte counts, mean corpuscular hemoglobin concentration and mean corpuscular hemoglobin in horses of 1-3 years age group (young stock) and on packed cell volume in adult female and male equids. Among biochemical indices, activities of enzymes were observed to be influenced both by age and sex. Creatine kinase, gamma glutamyl transferase, glutamate pyruvate transaminase, glutamic oxaloacetate transaminase and lactate dehydrogenase activities were significantly higher in young and adult equids than animals of other age groups in Kathiawari horses while activity of alkaline phosphatase was significantly higher in yearlings than equids belonging to other age groups in both male and females. However, activity of sorbitol dehydrogenase was unaltered due to both sex and age factor. Albumin, bilirubin direct, bilirubin total, cholesterol, creatinine, protein, triglyceride and uric acid were statistically different in various age and sex groups of horses. Calcium, magnesium and chloride contents were almost same in various age groups of male horses. Significantly higher levels of $T_3$ and $T_4$ were observed in both male and female yearlings as compared to equids of other age groups in both the sexes.

On-Line Monitoring of Low Biochemical Oxygen Demand Through Continuous Operation of a Mediator-Less Microbial Fuel Cell

  • MOON, HYUN-SOO;CHANG, IN-SEO;JANG, JAE-KYUNG;KIM, KYUNG-SHIK;LEE, JI-YOUNG;LOVITT, ROBERT W.;KIM, BYUNG-HONG
    • Journal of Microbiology and Biotechnology
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    • v.15 no.1
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    • pp.192-196
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    • 2005
  • Abstract Oligotrophic microbial fuel cells (MFCs) were tested for the continuous monitoring of low biochemical oxygen demand (BOD) by using artificial wastewater, containing glucose and glutamate, as check solution. Ten times diluted trace mineral solution was used to minimize the background current level, which is generated from the oxidation of nitrilotriacetate used as a chelating agent. The feeding rate of 0.53 ml/min could increase the sensitivity from 0.16 to 0.43 ${\mu}$A/(mg BOD/l) at 0.15 ml/min. The dynamic linear range of the calibration curve was between 2.0 and 10.0 mg BOD/l, and the response time to the change of 2 mg BOD/l was about 60 min. The current signal from an oligotroph-type MFCs increased with the increase in salts concentration, and the salt effect could be eliminated by 50 mM phosphate buffer.

Thermal Curing Property of Silicone Encapsulant Containing Quantum Dot Surrounded by Various Types of Ligands

  • Lee, Chae Sung;Kim, BeomJong;Jeon, Seongun;Han, Cheul Jong;Hong, Sung-Kyu
    • Bulletin of the Korean Chemical Society
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    • v.34 no.12
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    • pp.3787-3789
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    • 2013
  • In this study, the silicone thermal curing degree of the silicone-encapsulated quantum dot light emission diode was measured using the various types of chemical ligands around quantum dot. It was confirmed that the trioctyl phosphin oxide (TOPO) ligand around the quantum dot was responsible for dispersion of the quantum dot in silicone encapsulant and decline of the thermal curing degree of the silicone encapsulant. Also, it was confirmed that the thermal curing degree of silicone encapsulants containing the steric acid (SA) and the dodecanoic acid (DA) ligands were higher than the one of TOPO ligand.

Demonstration of Two Independent Dextranase and Amylase Active Sites on a Single Enzyme Elaborated by Lipomyces starkeyi KSM 22

  • LEE, SO-YOUNG;JIN-HA LEE;JOHN F. ROBYT;EUN-SEONG SEO;HYEN-JOUNG PARK;DOMAN KIM
    • Journal of Microbiology and Biotechnology
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    • v.13 no.2
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    • pp.313-316
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    • 2003
  • Lipomyces starkeyi KSM 22 elaborates an enzyme that has both dextranase and amylase activities in a single protein of 100 kDa. Competition studies, using different amounts of dextran and starch as substrates, gave a competition plot consistent with the hypothesis that the hydrolysis of dextran and starch occurs at two independent active sites, each specific for starch and dextran, respectively.

Biochemical properties and serotypes of pathogenic Escherichia coli isolated from poultry in Korea (가금 유래 병원성대장균의 생화학적 성상 및 혈청형)

  • Sung, Myung-Suk;Kim, Jin-Hyun;Ha, Jong-Su;Cho, Jae-Keun;Seol, Sung-Yong;Kim, Ki-Seuk
    • Korean Journal of Veterinary Research
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    • v.48 no.2
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    • pp.145-151
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    • 2008
  • This study was conducted to investigate biochemical properties and O group serotypes of pathogenic 203 Escherichia (E.) coli isolates from poultry with collibacillosis in Korea during the period from April 2003 to December 2005. Biochemical and fermentative properties of 203 isolates of E. coli tested were in accordance with Cowan and Steel's classification standard. One hundred and forty one isolates (69.5%) could be classified into a total of 20 O serotypes. Among them, the predominant O groups were O78 (32.5%), O88 (7.8%), O15 (6.8%), O141 (6.4%), and O158 (3.0%) in decreased order. Other infrequently encountered serogroups included : O8 (2%), O161 (2%), O20 (1.5%), O125 (1.5%), O2 (1%). And O6, O18, O24, O46, O76, O109, O119, O138, O139 and O148 had a frequency of 0.5%, respectively. Sixty two isolates (30.5%) were non-typeable with standard 173 O antisera used in this study.

Subcutaneous Injection of Bee Venom in Wistar Rats: effects on blood cells and biochemical parameters

  • Yousefpoor, Yaser;Osanloo, Mahmoud;Mirzaei-Parsa, Mohamad Javad;Najafabadi, Mohammad Reza Hoseini;Hashemi, Seyyed Mohammad;Abbasifard, Mitra
    • Journal of Pharmacopuncture
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    • v.25 no.3
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    • pp.250-257
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    • 2022
  • Objectives: Bee venom (BV) therapy is performed by a bee sting or subcutaneous injection of BV. However, there is not much information on the effect of BV on blood parameters after entering the body. This project aimed to assess the side effects of subcutaneous BV injections in healthy rats by measuring the hematological and biochemical parameters. Methods: Various amounts of BV, including 100, 200, and 500 (㎍/day), were subcutaneously injected into rats for 30 days. The results showed that BV affected the metabolism of the liver, kidney, and glands. Results: An increase in blood sugar and a decrease in other biochemical parameters, including cholesterol, triglyceride, urea, creatinine AST, ALT, ALP, and phosphorous, were observed. Results also showed increased counts of white blood cells, neutrophils (%), and platelets and decreased levels of red cells, hemoglobin, and hematocrit. Conclusion: This study demonstrates that BV therapy in medical clinics requires routine care and testing to prevent eventual metabolic and anemia side effects.