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http://dx.doi.org/10.3831/KPI.2022.25.3.250

Subcutaneous Injection of Bee Venom in Wistar Rats: effects on blood cells and biochemical parameters  

Yousefpoor, Yaser (Department of Medical Biotechnology, School of Paramedical Sciences, Torbat Heydariyeh University of Medical Sciences)
Osanloo, Mahmoud (Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Fasa University of Medical Sciences)
Mirzaei-Parsa, Mohamad Javad (Department of Medical Nanotechnology, Faculty of Allied Medical Sciences, Kerman University of Medical Sciences)
Najafabadi, Mohammad Reza Hoseini (Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences)
Hashemi, Seyyed Mohammad (Khalil Abad Health Center, Mashhad University of Medical Sciences)
Abbasifard, Mitra (Immunology of Infectious Diseases Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences)
Publication Information
Journal of Pharmacopuncture / v.25, no.3, 2022 , pp. 250-257 More about this Journal
Abstract
Objectives: Bee venom (BV) therapy is performed by a bee sting or subcutaneous injection of BV. However, there is not much information on the effect of BV on blood parameters after entering the body. This project aimed to assess the side effects of subcutaneous BV injections in healthy rats by measuring the hematological and biochemical parameters. Methods: Various amounts of BV, including 100, 200, and 500 (㎍/day), were subcutaneously injected into rats for 30 days. The results showed that BV affected the metabolism of the liver, kidney, and glands. Results: An increase in blood sugar and a decrease in other biochemical parameters, including cholesterol, triglyceride, urea, creatinine AST, ALT, ALP, and phosphorous, were observed. Results also showed increased counts of white blood cells, neutrophils (%), and platelets and decreased levels of red cells, hemoglobin, and hematocrit. Conclusion: This study demonstrates that BV therapy in medical clinics requires routine care and testing to prevent eventual metabolic and anemia side effects.
Keywords
bee venom; subcutaneous injection; biochemical; hematological;
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1 Mankiewicz JL, Deck CA, Taylor JD, Douros JD, Borski RJ. Epinephrine and glucose regulation of leptin synthesis and secretion in a teleost fish, the tilapia (Oreochromis mossambicus). Gen Comp Endocrinol. 2021;302:113669.   DOI
2 Alqutub AN, Masoodi I, Alsayari K, Alomair A. Bee sting therapy-induced hepatotoxicity: a case report. World J Hepatol. 2011;3(10):268-70.   DOI
3 Rej R. Aminotransferases in disease. Clin Lab Med. 1989;9(4):667-87.   DOI
4 Li X, Zhang D, Bryden W. Calcium and phosphorus metabolism and nutrition of poultry: are current diets formulated in excess? Anim Prod Sci. 2017;57(11):2304-10.   DOI
5 Kutlu H, Avci E, Ozyurt F. White blood cells detection and classification based on regional convolutional neural networks. Med Hypotheses. 2020;135:109472.   DOI
6 Abbasifard M, Yousefpoor Y, Amani A, Arababadi MK. Topical bee venom nano-emulsion ameliorates serum level of endothelin-1 in collagen-induced rheumatoid arthritis model. BioNanoScience. 2021;11:810-5.   DOI
7 de Jonge G, Dos Santos TL, Cruz BR, Simionatto M, Bittencourt JIM, Krum EA, et al. Interference of in vitro hemolysis complete blood count. J Clin Lab Anal. 2018;32(5):e22396.   DOI
8 Rodriguez-Acosta A, Vega J, Finol HJ, Pulido-Mendez M. Ultrastructural alterations in cortex of adrenal gland caused by the toxic effect of bee (Apis mellifera) venom. J Submicrosc Cytol Pathol. 2003;35(3):309-14.
9 Lee JD, Park HJ, Chae Y, Lim S. An overview of bee venom acupuncture in the treatment of arthritis. Evid Based Complement Alternat Med. 2005;2(1):79-84.   DOI
10 Yousefpoor Y, Amani A, Divsalar A, Vafadar MR. Topical delivery of bee venom through the skin by a water-in-oil nanoemulsion. Nanomed J. 2022;9(2):131-7.
11 Pu N, Gao S, Xu Y, Zhao G, Lv Y, Nuerxiati A, et al. Alkaline phosphatase-to-albumin ratio as a prognostic indicator in pancreatic ductal adenocarcinoma after curative resection. J Cancer. 2017;8(16):3362-70.   DOI
12 Park JH, Yim BK, Lee JH, Lee S, Kim TH. Risk associated with bee venom therapy: a systematic review and meta-analysis. PLoS One. 2015;10(5):e0126971.   DOI
13 Zalat S, Nabil Z, Hussein A, Rakha M. Biochemical and haematological studies of some solitary and social bee venoms. Egypt J Biol. 1999;1:57-71.
14 Zhang S, Liu Y, Ye Y, Wang XR, Lin LT, Xiao LY, et al. Bee venom therapy: potential mechanisms and therapeutic applications. Toxicon. 2018;148:64-73.   DOI
15 Kamal A. Estimation of blood urea (BUN) and serum creatinine level in patients of renal disorder. Indian J Fundam Appl Life Sci. 2014;4(4):199-202.
16 Marin-Garcia PJ, Lopez-Lujan MC, Rodenas L, Martinez-Paredes E, Blas E, Pascual JJ. Plasma urea nitrogen as an indicator of amino acid imbalance in rabbit diets. World Rabbit Sci. 2020;28(2):63-72.   DOI
17 Mundy GR, Guise TA. Hormonal control of calcium homeostasis. Clin Chem. 1999;45(8 Pt 2):1347-52.   DOI
18 Kang HS, Kim SJ, Lee MY, Jeon SH, Kim SZ, Kim JS. The cardiovascular depression caused by bee venom in Sprague-Dawley rats associated with a decrease of developed pressure in the left ventricular and the ratio of ionized calcium/ionized magnesium. Am J Chin Med. 2008;36(3):505-16.   DOI
19 Son DJ, Lee JW, Lee YH, Song HS, Lee CK, Hong JT. Therapeutic application of anti-arthritis, pain-releasing, and anti-cancer effects of bee venom and its constituent compounds. Pharmacol Ther. 2007;115(2):246-70.   DOI
20 Yousefpoor Y, Amani A, Divsalar A, Mousavi SE, Torbaghan YE, Emami O. Assessment of hemolytic activity of bee venom against some physicochemical factors. J Asia Pac Entomol. 2019;22:1129-35.   DOI
21 Ali M. Studies on bee venom and its medical uses. Int J Adv Res Technol. 2012;1(2):69-83.
22 Yousefpoor Y, Bolouri B, Bayati M, Shakeri A, Eskandari Y. The combined effects of Aloe vera gel and silver nanoparticles on wound healing in rats. Nanomed J. 2016;3(1):57-64.
23 World Health Organization. General guidelines for methodologies on research and evaluation of traditional medicine. Geneva: World Health Organization; 2000. 71 p.
24 Wehbe R, Frangieh J, Rima M, El Obeid D, Sabatier JM, Fajloun Z. Bee venom: overview of main compounds and bioactivities for therapeutic interests. Molecules. 2019;24(16):2997.   DOI
25 Benton AW, Morse RA, Stewart JD. Venom collection from honey bees. Science. 1963;142(3589):228-30.   DOI
26 Mohammed ZI, Hassan AJ. Effect of bee venom on some blood and biochemical parameters in formaldehyde induced arthritis male rats in comparison with prednisolone drug. J Phys Conf Ser. 2019;1234:012066.   DOI
27 Yousefpoor Y, Amani A, Divsalar A, Mousavi SE, Shakeri A, Sabzevari JT. Anti-rheumatic activity of topical nanoemulsion containing bee venom in rats. Eur J Pharm Biopharm. 2022;172:168-76.   DOI
28 Elkotby D, Hassan AK, Emad R, Bahgat I. Histological changes in islets of Langerhans of pancreas in alloxan-induced diabetic rats following Egyptian honey bee venom treatments. Int J Pure Appl Zool. 2018;6(1):1-6.
29 Mousavi SM, Imani S, Haghighi S, Mousavi SE, Karimi A. Effect of Iranian honey bee (Apis mellifera) venom on blood glucose and insulin in diabetic rats. J Arthropod Borne Dis. 2012;6(2):136-43.
30 Pleuvry BJ. Pharmacological control of blood sugar. Anaesth Intensive Care Med. 2005;6(10):344-6.   DOI
31 Katsumi A, Abe A, Tamura S, Matsushita T. Anemia in older adults as a geriatric syndrome: a review. Geriatr Gerontol Int. 2021;21(7):549-54.   DOI
32 Fletcher JE, Tripolitis L, Beech J. Bee venom melittin is a potent toxin for reducing the threshold for calcium-induced calcium release in human and equine skeletal muscle. Life Sci. 1992;51(22):1731-8.   DOI
33 Lim C, Park S, Sun S, Lee K. Research on Korean pharmacopuncture in South Korea since 2007. J Pharmacopuncture. 2014;17(4):15-21.   DOI
34 Darwish SF, El-Bakly WM, Arafa HM, El-Demerdash E. Targeting TNF-α and NF-κB activation by bee venom: role in suppressing adjuvant induced arthritis and methotrexate hepatotoxicity in rats. PLoS One. 2013;8(11):e79284.   DOI
35 Sarma PR. Red cell indices. In: Walker HK, Hall WD, Hurst JW, editors. Clinical methods: the history, physical, and laboratory examinations. 3rd ed. Boston (MA): Butterworths; 1990.
36 Daher Ede F, da Silva Junior GB, Bezerra GP, Pontes LB, Martins AM, Guimaraes JA. Acute renal failure after massive honeybee stings. Rev Inst Med Trop Sao Paulo. 2003;45(1):45-50.   DOI
37 Orsolic N. Bee venom in cancer therapy. Cancer Metastasis Rev. 2012;31(1-2):173-94.   DOI
38 Tappy L. Metabolism of sugars: a window to the regulation of glucose and lipid homeostasis by splanchnic organs. Clin Nutr. 2021;40(4):1691-8.   DOI