[KSCI] Korea Science Citation Index Service

Demonstration of Two Independent Dextranase and Amylase Active Sites on a Single Enzyme Elaborated by Lipomyces starkeyi KSM 22

LEE, SO-YOUNG (Department of Material and Biochemical Engineering, Chonnam National University)
JIN-HA LEE (Engineering Research Institute, Chonnam National University)
JOHN F. ROBYT (Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, USA)
EUN-SEONG SEO (Department of Material and Biochemical Engineering, Chonnam National University)
HYEN-JOUNG PARK (Department of Material and Biochemical Engineering, Chonnam National University)
DOMAN KIM (Department of Material and Biochemical Engineering, Chonnam National University)

Publication Information

Journal of Microbiology and Biotechnology / v.13, no.2, 2003 , pp. 313-316 More about this Journal

Abstract

Lipomyces starkeyi KSM 22 elaborates an enzyme that has both dextranase and amylase activities in a single protein of 100 kDa. Competition studies, using different amounts of dextran and starch as substrates, gave a competition plot consistent with the hypothesis that the hydrolysis of dextran and starch occurs at two independent active sites, each specific for starch and dextran, respectively.

Keywords

Dextranase; amylase; Lipomyces starkeyi; active site; competition plot;

Citations & Related Records

Times Cited By KSCI : 8 (Citation Analysis)
Times Cited By Web Of Science : 26 (Related Records In Web of Science)

Reference
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