• 한국미생물·생명공학회지
• /
• 제23권3호
• /
• pp.297-303
• /
• 1995

Antitumor antibiotic material was produced by Streptomyces sp. YBE-316 which was isolated from soil, and the optimal culture conditions for the antitumor antibiotic material production were as follows; 2.0% (w/v) sucrose, 0.8% (w/v) polypeptone, 0.4% (w/v) yeast extract, 0.2% (w/v) K$_{2}$HPO$_{4}$, pH 7.0, at 30$\circ$C, 150 rpm and for 100 hours culture. The antitumor antibiotic material had strong antitumor antibiotic activities against most testing tumor cell lines, gram positive and negative bacteria, yeasts, and, especially, Penicillium chrysogenum in fungi.

• Journal of Microbiology and Biotechnology
• /
• 제13권4호
• /
• pp.483-486
• /
• 2003

3'-[S-Methyl-cysteinyl]-3'-amino-3'-deoxy-N,N- dimethyl adenosine, cystocin, is a biosynthesized antibiotic material newly identified from Streptomyces sp. GCA0001. Its structure was found to be similar to puromycin, where the terminal tyrosine is replaced by a methyl cysteine. NMR data prove that the 3-ammo ribose is connected to dimethylaminopurine through the anomeric carbon at 1'-carbon. The methyl cysteinyl unit is connected to the amino unit of ribose by peptide bond. The verification of the structure was performed by comparing the puromycin nucleosides resulted from the hydrolysis of cystocin and puromycin, respectively. Antibiotic activity of cystocin against Streptococcus was found to be two times more potent than that of puromycin.

• 생명과학회지
• /
• 제19권5호
• /
• pp.676-679
• /
• 2009

미생물들 사이에 존재하는 수평적 유전자 전달을 이용하여 Jurkat T cell에 대하여 새로운 항생물질을 생산하기 위해 토양박테리아 AY2000과 여러 종류의 항생물질 생산 균주인 Streptomyces griseus의 공배양을 수행하였다. MTT assay를 수행하여 세포 독성을 실험을 하였을 때 공배양 상등액은 각각 배양 하였을 때보다 높은 세포독성을 보였고 또한 48시간 배양 하였을 때 가장 높은 활성을 나타내는 것으로 나타났다. 더욱이 DAPI 염색을 하였을 때 Jurkat T cell 세포의 세포핵의 변화도 관찰 되었다. 이런 결과는 공배양 상등액에 새로운 항생물질이 생성되었음을 보여주었고 이런 방법으로 새로운 항생물질 생산에 이용되어 질수 있음을 의미한다.

• Journal of Microbiology and Biotechnology
• /
• 제12권1호
• /
• pp.18-24
• /
• 2002

The present study was performed to investigate the excellent microbial anticaries substance, aminoglycoside antibiotic, which is more effective than chlorhexidine for the treatment of dental caries. The aminoglycoside antibiotic against Streptococcus mutans JC-2 from a novel alkaliphilic Bacillus alkalophilshaggy JY-827 exhibited no significant difference at the treatment concentration of $2.5{\times}10^{-7}M$, however, it inhibited the activity of the Streptococcus mutans glucosyltransferase by 70.2% and 99.8% at the concentrations of $2.5{\times}10^{-7}$M\;and\;2.5{\times}10^{-6}M$, respectively. Lineweaver-Burk plot of the inhibitory aminoglycoside antibiotic showed competitive inhibition, with $K_i$ value of $6.4{\times}10^{-6}$ M. The aminoglycoside antibiotic did not show any cytotoxicity against human gingival cells. To evaluate the industrial applicability of the aminoglycoside antibiotic, a toothpaste containing this substance was prepared and tested on the extracted human teeth. The inhibitory rate of tooth calcification and calcium ion elution by the aminoglycoside antibiotic were 50% and 2.5 times, respectively. These results suggested that the aminoglycoside antibiotic from Bacillus alkalophilshaggy JY-827 is an effective agent against dental caries.

• 한국포장학회지
• /
• 제5권2호
• /
• pp.31-37
• /
• 1999

본 연구에서는 새로운 개념의 항균처리 기술을 도입하여 항균 물질을 플라스틱 용기에 혼입하지 않고도 항균기능이 발휘되도록 하는 기술을 개발하고자 하였다. 기술의 요체는 항균기능정보를 물에 각인한 다음에 이를 플라스틱 제조용 원료 수지에 전사하여 최종 제품에서도 같은 기능이 나타나도록 한 것이다. 이러한 개념에 입각한 기능 정보 각인 기술의 실용화 가능성을 검토하기 위하여 시제품을 만들어 항균 실험을 수행한 결과 세균 증식 억제율이 75% 전후의 수준인 것으로 나타났다. 앞으로 이러한 기술이 보다 더 다듬어지고 그 작용기전이 밝혀진다면 더욱 뛰어난 제품이 개발될 가능성이 높다고 하겠다.

• 도시과학
• /
• 제8권2호
• /
• pp.1-5
• /
• 2019

China is the great powers of use and production of antibiotics.The current process of sewage treatment plants can not effectively remove antibiotics in water. Chinese scholars have detected different kinds of antibiotics in major waters of the country, which have potential harm to human body. Among all kinds of antibiotic treatment technologies, adsorption removal technology has the advantages of simple operation, low cost and high removal efficiency. It is a widely concerned antibiotic removal technology. However, at present, few materials have been put into practical application, and more materials with low cost and high efficiency need to be found. Different adsorptive materials have different adsorptivity to different antibiotics. For different antibiotics, different adsorptive materials can be integrated in the future, and the theory can be extended to application.

• Journal of Electrochemical Science and Technology
• /
• 제15권1호
• /
• pp.152-160
• /
• 2024

In this study, ZnCo₂O₄ nanoparticles were synthesized via the coprecipitation method using different annealing temperatures from 200℃ to 800℃. By varying the treatment temperature, the morphology changed from amorphous to tetragonal, and finally to polygonal particles. As temperature increased, the sizes of the nanoparticles also changed from 5 nm at 200℃ to approximately 500 nm at 800℃. The fabricated material was used to modify the working electrode of a screen-printed carbon electrode (SPE), which was subsequently used to survey the detection performance of the antibiotic, chloramphenicol (CAP). The electrochemical results revealed that the material exhibits a good response to CAP. Further, the sample that annealed at 600℃ displayed the best performance, with a linear range of 1-300 μM, and a limit of detection (LOD) of 0.15 μM. The sensor modified with ZnCo₂O₄ also exhibited the potential for utilitarian application when the recovery in a real sample was above 97%.

• Journal of Korean Neurosurgical Society
• /
• 제64권2호
• /
• pp.297-308
• /
• 2021

Objective : Shunt infection is a common complication while treating hydrocephalus. The antibiotic-impregnated shunt catheter (AISC) was designed to reduce shunt infection rate. A meta-analysis was conducted to study the effectiveness of AISCs in reduction of shunt infection in terms of age, follow-up time and high-risk patient population. Methods : This study reviewed literature from three databases including PubMed, EMBASE, and Cochrane Library (from 2000 to March 2019). Clinical studies from controlled trials for shunt operation were included in this analysis. A subgroup analysis was performed based on the patient's age, follow-up time and high-risk population. The fixed effect in RevMan 5.3 software (Cochrane Collaboration) was used for this meta-analysis. Results : This study included 19 controlled clinical trials including 10105 operations. The analysis demonstrated that AISC could reduce the infection rate in shunt surgery compared to standard shunt catheter (non-AISC) from 8.13% to 4.09% (odds ratio [OR], 0.48; 95% confidence interval [CI], 0.40-0.58; p=0.01; I2=46%). Subgroup analysis of different age groups showed that AISC had significant antimicrobial effects in all three groups (adult, infant, and adolescent). Follow-up time analysis showed that AISC was effective in preventing early shunt infections (within 6 months after implant). AISC is more effective in high-risk population (OR, 0.24;95% CI, 0.14-0.40; p=0.60; I2=0%) than in general patient population. Conclusion : The results of meta-analysis indicated that AISC is an effective method for reducing shunt infection. We recommend that AISC should be considered for use in infants and high-risk groups. For adult patients, the choice for AISC could be determined based on the treatment cost.

• 한국연초학회지
• /
• 제20권2호
• /
• pp.172-177
• /
• 1998

The culture filtrate of Streptomyces sp. B25, which was identified in this experiment, was tested for the control of tobacco mosaic virus (TMV) with the susceptible tobacco cultivar, NC 82, under the field conditions following the preliminary examination of its characters for TMV control. Control efficacy of the culture filtrate against TMV infection continued over 50% up to 6 days after treatment, and its systemic effect was about 30% of the direct effect. In field conditions control efficacy of the culture filtrate against TMV infection was 95.3 % at 2 weeks after TMV inoculation, and decreased to 58.3 % at 3 weeks after inoculation. Five fold-dilution of the culture filtrate showed about half of the control efficacy by the stock culture filtrate. Analysis of the antibiotic material responsible for the inhibition of TMV infection through nuclear magnetic resonance experiments revealed that the antibiotic is antimycin $A_1$, which is firstly reported as an anti-phytoviral antibiotic in this experiment.

• 한방안이비인후피부과학회지
• /
• 제30권2호
• /
• pp.100-111
• /
• 2017

Objectives : The purpose of this study was to research the antibiotic, antioxidant and whitening effects of Jwa Kum-Whan(JKW) and Soo Ryeon-Whan(SRW). Both Jwa Kum-Whan and Soo Ryeon-Whan are composed of Coptidis rhizoma and Evodiae fructus, but the ratios of the two species are different. JKW is composed of Coptidis rhizoma and Evodiae fructus by ratio of 6:1 and SRW's ratio is 1:1. Methods : Antibiotic activities of JKW and SRW's water extracts were studied by paper disc diffusion method. Four kinds of bacteria were applied in paper disc, and each extract was dropped, individually. The diameter of inhibition zone was measured. DPPH assay was used for studying free radical scavenging activity. DPPH is quantitatively decolorized from purple color by antioxidant material. Change of color was measured by spectrophotometer. Whitening activity was analyzed by tyrosinase inhibition assay. Tyrosinase is major enzyme for control process of making eumelanin. Optical Density was measured by spectrophotometer. Results : Candida albicans and Staphylococcus aureus's inhibition zone were made large available by Both prescriptions. Inhibition zone's diameter of JKW was preferable to SRW's. Radical scavenging activity was better at SRW, but JKW's activity was available, too. Tyrosinase inhibition activity was found out available only for JKW, not for SRW. Conclusions : JKW had antibiotic effects for Candida albicans, Staphylococcus aureus. And had antioxidant, whitening effects. SRW had antibiotic, antioxidant activities but not whitening effect.