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http://dx.doi.org/10.5352/JLS.2009.19.5.676

Anti-cancer Activity of Supernatant of Rahnella aquatilis AY 2000 Cocultured with Streptomyces griseus  

Kim, Ji-Hyun (Department of Biotechnology and Bioengineering, Dong Eui university)
Kim, Kwang-Hyeon (Department of Biotechnology and Bioengineering, Dong Eui university)
Lee, Jong-Hwan (Department of Life Science and Biotechnology, Dong Eui university)
Publication Information
Journal of Life Science / v.19, no.5, 2009 , pp. 676-679 More about this Journal
Abstract
In order to produce a new antibiotic material against Jurkat T cells using horizontal gene transfer among microbes, co-cultures between soil bacteria AY2000 and the multiple antibiotic producer S. griseus was carried out. It showed that the highest active substance against Jurkat T cells was produced at 48 hr of co-culture time with MTT assay. Moreover, a morphological change of nuclear of Jurkat T cells treated with co-cultured substance was observed in DAPI staining. This result suggests that a new material was produced with co-culture supernatant, and that co-culture between microboes can develop new antibiotic materials.
Keywords
Coculture; horizontal gene transfer; AY2000; S. griseus; antibiotic material;
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