• Journal of Dairy Science and Biotechnology
• /
• 제40권1호
• /
• pp.23-34
• /
• 2022

In this study, to determine the importance of lactic acid bacteria (LAB) in Kombucha fermentation, biological functions, such as organic acid production and anti-inflammatory and antibacterial activities, of Kombucha, with or without LAB inoculation, were evaluated. Lactobacillus paracasei DK215, Saccharomyces cerevisiae C3, and Acetobacter pasteurianus P2 were selected as the inoculants. Organic acids were measured every 3 days from the end of fermentation using HPLC; the organic acid content of LAB-inoculated Kombucha was relatively high. Samples with or without LAB inoculation showed high antibacterial activity against Escherichia coli. The MTT assay results indicated no significant difference in concentration difference and cell death. In the NO production test, compared with the uninoculated Kombucha sample, the LAB-inoculated Kombucha sample exhibited a value similar to that of the group without LPS treatment. The levels of cytokine (IL-1α, IL-6, TNF-α) production were significantly lower than those of the LPS(+) group, indicating the anti-inflammatory activity potential of the Kombucha sample. This improvement in the biological function of the LAB-inoculated Kombucha further verifies the value of LAB in the fermented food and beverage industry.

• Biomolecules & Therapeutics
• /
• 제30권6호
• /
• pp.593-602
• /
• 2022

The human papillomavirus (HPV)-18 E7 (E7) oncoprotein is a major transforming protein that is thought to be involved in the development of cervical cancer. It is well-known that E7 stimulates tumour development by inactivating pRb. However, this alone cannot explain the various characteristics acquired by HPV infection. Therefore, we examined other molecules that could help explain the acquired cancer properties during E7-induced cancer development. Using the yeast two-hybrid (Y2H) method, we found that the Elk-1 factor, which is crucial for cell proliferation, invasion, cell survival, anti-apoptotic activity, and cancer development, binds to the E7. By determining which part of E7 binds to which domain of Elk-1 using the Y2H method, it was found that CR2 and CR3 of the E7 and parts 1-206, including the ETS-DNA domain of Elk-1, interact with each other. As a result of their interaction, the transcriptional activity of Elk-1 was increased, thereby increasing the expression of target genes EGR-1, c-fos, and E2F. Additionally, the colony forming assay revealed that overexpression of Elk-1 and E7 promotes C33A cell proliferation. We expect that the discovery of a novel E7 function as an Elk-1 activator could help explain whether the E7 has novel oncogenic activities in addition to p53 inactivation. We also expect that it will offer new methods for developing improved strategies for cervical cancer treatment.

• 한국식품과학회지
• /
• 제7권2호
• /
• pp.61-68
• /
• 1975

산막효모(産膜酵母)가 형성(形成)된 제품(製品)간장에서 산막생성(産膜生成)과 발효력(醱酵力)이 강(强)한 효모(酵母)를 분리(分離)하고 Lodder 들의 효모분류법(酵母分類法)에 따라 Saccharomyces rouxii (film forming yeast)로 동정(同定)하였으며 이효모(酵母)들의 발효현상(發酵現象) 및 생리적(生理的) 특성(特性)은 다음과 같았다. 1. 간장발효실험(發酵實驗)에서 간장중(中)에 함유(含有)된 당분(糖分)을 모두 발효(醱酵)시켜 고형분(固形分)은 감소(減少)되고 총질소(總窒素), 색도(色度)를 감소(減少)시켰다. 2. 방부력실험(防腐力實驗)에서 butylparaben 은 60 ppm, sodium benzoate 800 ppm, sodium propionate 2,400 ppm, menadione 165 ppm, potassium sorbate 500 ppm, sorbic acid 300 ppm 이상(以上)의 농도(濃度)에서 이들균(菌)의 생육(生育)이 억제(抑制)되었고 에칠알콜 첨가구(添加區)에서는 3%에서도 방부력(防腐力)이 없었다. 3. 2%(w/v) glucose yeast extract peptone 배지(培地)에서 이들균(菌)의 최적식고농도(最適食?濃度)는 5%, 최적배양온도(最適培養溫度)는 $30^{\circ}C$, 사멸온도(死滅溫度)는 NO-1, 3균(菌)이 $62^{\circ}C$ (10분간(分間) 살균처리(殺菌處理)), NO-2, 4균(菌)이 $65^{\circ}C$ 이었으며 최적(最適) pH 는 5. 0이었다. 4. Gas chromatography 에 의(依)한 피막(皮膜)간장의 향기성분(香氣成分)은 표준(標準)간장에 비(比)해 저비점(低沸點)의 물질(物質)이 많으며 이 성분(成分)들이 간장의 풍미열화(風味劣化)에 기여(寄與)하는 것으로 생각되었다.

• Biomolecules & Therapeutics
• /
• 제21권5호
• /
• pp.391-397
• /
• 2013

In the present study, we aimed to analyze the antinociceptive, immunomodulatory and antipyretic activities of nymphayol were investigated in wistar rats and mice. Antinociceptive effect was evaluated by acetic acid induced writhing, formalin induced paw licking and hot-plate tests. Immunomodulatory activity was assessed by neutrophil adhesion test, humoral response to sheep red blood cells, delayed-type hypersensitivity, phagocytic activity and cyclophosphamide induced myelosuppression. Antipyretic activity was evaluated by yeast induced hyperthermia in rats. Nymphayol produced significant (p<0.05) antinociceptive activity in acetic acid induced writhing response and late phase of the formalin induced paw licking response. Pre-treatment with nymphayol (50 mg/kg, oral) evoked a significant increase in neutrophil adhesion to nylon fibres. The augmentation of humoral immune response to sheep red blood cells by nymphayol (50 mg/kg) was evidenced by increase in antibody titres in rats. Oral administration of nymphayol (50 mg/kg) to rats potentiated the delayed-type hypersensitivity reaction induced by sheep red blood cells. Treatment with nymphayol showed a significant (p<0.05) reduction in pyrexia in rats. The results suggest that nymphayol possesses potent anti-nociceptive, immunomodulatory and antipyretic activities.

• 생약학회지
• /
• 제38권4호
• /
• pp.363-371
• /
• 2007

Schizandrae Fruits has been used as a traditional Oriental medicine for treatment of many stress-induced diseases. In the present study, we investigated inhibitory activity of enzymatic hydrolysate of Schizandrae fructus (SC-EX) in growth of tested intestinal microorganism and activity of bowel inflammation related enzyme. SC-EX was added to the proteose peptone-yeast extract-fildes (PYF) media to investigation the effect on the growth of type culture of intestinal microorganism. The growth of lactic acid bacteria such as Bifidobacterium species and Lactobacillus species was accelerated by more than 3% concentration of SC-EX. But, growth of harmfulness bacteria such as E.coli, Clostridium sp. Staphylococcus sp. Streptococcus sp. was inhibited by more than 3% concentration of SC-EX. Also, SC-EX was exhibited a concentration-dependent inhibitory activity of the bowel inflammation related enzymes. The SC-EX was showed 76% and 92% inhibitory activity of 5-lipoxygenase and cyclooygenase at 5% additional concentration respectively. Our results indicated that SC-EX may possess improvement effect on the intestinal flora and Anti-inflammatory effect on the bowel.

• KSBB Journal
• /
• 제23권5호
• /
• pp.419-424
• /
• 2008

본 연구에서 당뇨병의 예방, 치료제 및 건강기능 식품으로서 산업적 활용가능성을 평가하기 위한 기초연구로서 번행초 추출물을 사용하여 당뇨관련 효소인 $\alpha$-amylase, $\alpha$-glucosidase, $\beta$-glucosidase 그리고 aldose reductase에 대한 저해활성을 조사하였다. 번행초의 HX 분획물은 인간 타액, 돼지 췌장 $\alpha$-amylase에 대해 5 mg/mL 농도에서 각각 62.23%, 88.74%로 높은 저해효과를 나타냈으며, $\alpha$-glucosidase에 대해서도 HX 분획물이 1 mg/mL 농도에서 60.27%로 높은 저해효과를 나타냄으로써 현재 혈당강하제로 사용되고 있는 acarbose와 1-deoxynorjirimycin보다 높은 억제 효과를 보였다. 그리고 만성 합병증에 의한 시력장애, 신경성장애, 신장기능장애, 심장기능 장애 등의 심각한 당뇨병성 합병증 유발과 관련이 있는 aldose reductase에 대한 억제활성을 조사한 결과, 1 mg/mL 농도에서 번행초 DCM 분획과 HX 분획물이 각각 51.95%, 47.22%로 높은 저해활성을 나타냄으로써 당뇨병과 그로 인한 합병증의 예방에도 효과가 있는 것으로 판단된다. 전 세계적으로 당뇨병 환자가 증가추세에 있어 당뇨병 치료제의 시장 규모는 더욱 확대될 가능성이 높은 가운데 지금까지 보고되지 않은 번행초의 항당뇨 효능의 우수성을 검증함으로써 바이오산업육성뿐만 아니라 신소득 작물로 순화재배를 통한 농가소득 향상에도 기여할 것으로 기대된다.

• 한국식품과학회지
• /
• 제42권5호
• /
• pp.549-553
• /
• 2010

본 연구에서는 다양한 효모 배양액을 자가소화 시킨 후 관능검사를 통해 1차적으로 8종(S. cerevisiae 3종, C. utilis 4종 및 Z.rouxii 1종)을 선별하였다. 이 중 정미성과 균체 성장율을 고려하여 C. utilis인 SEM-Y8을 선정하여 고글루타치온 생산을 위한 균주 개량을 수행하였다. 두 번의 UV 처리를 통해 SEM-Y8보다 글루타치온 생성능이 2.0배 증가된 SY8-M2-1 개량 효모 균주를 얻었으며, 선택배지(4.0% yeast extract, 1.5% glucose, 0.04% $NH_4H_2PO_4$, 0.04% L-cystein)를 사용함으로써 글루타치온 함량을 1.5배 더 향상시킬 수 있었다. 이상의 조건에서 얻어진 고글루타치온 함유 효모추출물은 마늘과 양파엑기스보다 우수한 감칠맛과 부드럽고 깊은 맛을 나타내었으며, SEM-Y8 대비 3.2배 향상된 라디컬 소거능을 나타내었다.

• Journal of Plant Biotechnology
• /
• 제34권4호
• /
• pp.313-322
• /
• 2007

Extract of Japanese red pine needles has been used in Asia pacific regions since long periods believing its valuable properties as tonic and ability of curing diseases of unidentified symptoms. Some selective compounds present in the extract and their effects were analyzed. Carbohydrates and vitamin c were identified using HPLC; terpenoid compounds by GC-MS; anti-bacterial analysis by paper discs, plates count and gastrointestinal motility by whole cell patch clamp. The extract is a mixture of compounds therefore its diverse effect was expected. Self-fermentation in extract proceeds after spontaneous appearance of yeast strains without inoculation. Effects and composition of the extract vary with varying period of self-fermentation. Extract inhibits the growth of bacteria dose dependently exhibiting its antibacterial properties however effectiveness increases with increase in fermentation period. The extract also can modulate gastrointestinal motility in murine small intestine by modulating pace maker currents in ICC mediated through ATP sensitive potassium channel.

• Journal of Applied Biological Chemistry
• /
• 제62권2호
• /
• pp.137-141
• /
• 2019

본 연구에서 금잔화(Calendula arvensis)에 대한 다양한 생리, 화학적 활성을 시험하여 기능성소재 가능성을 검토하였다. 금잔화 추출물은 낮은 세포독성을 나타냈다. 세포독성이 거의 없는 농도에서 금잔화 추출물 처리 시, 산화방지 활성, tyrosinase 활성저해 및 멜라닌 함량 저하를 보여주었다. 금잔화 추출물 농도에 따라 tyrosinase 단백질 발현이 억제되었으며, 이는 금잔화 추출물이 tyrosinase의 활성 및 단백질 발현을 억제시키거나 혹은 멜라노좀 수송 저해 등 다른 요인에 의해 멜라닌 합성을 저해하는 것으로 생각된다. 또한, 금잔화 추출물 처리 시, 항균활성이 나타남을 확인하였다. 따라서 금잔화 추출물은 향후 기능성 소재에 활용할 수 있는 효과적인 물질이 될 수 있다고 판단된다.

• Journal of Microbiology and Biotechnology
• /
• 제32권10호
• /
• pp.1315-1324
• /
• 2022

Fermentation is an effective process for providing various beneficial effects in functional beverages. Lactic acid bacteria and yeast fermentation-based biotransformation contribute to enhancement of nutritional value and digestibility, including lactose intolerance reduction and control of infections. In this study, the probiotic fermented fruit juice (PFJ) was produced by Lactobacillus plantarum TISTR 1465, Lactobacillus salivarius TISTR 1112, and Saccharomyces boulardii CNCM I-745 while mixed fruit juice (MFJ) was used as the basic medium for microorganism growth. The potential function, the anti-salmonella activity of PFJ, was found to be effective at 250 mg/ml of MIC and 500 mg/ml of MBC. Biofilm inhibition was performed using the PFJ samples and showed at least 70% reduction in cell attachment at the MIC concentration of Salmonella Typhi DMST 22842. The antioxidant activities of PFJ were determined and the results revealed that FSB.25 exhibited 78.40 ± 0.51 mM TE/ml by FRAP assay, while FPSB.25 exhibited 3.44 ± 0.10 mM TE/ml by DPPH assay. The volatile compounds of PFJ were characterized by GC-MS, which identified alcohol, aldehyde, acid, ester, ketone, phenol, and terpene. The most abundant organic acid and alcohol detected in PFJ were acetic acid and 2-phenylethanol, and the most represented terpene was β-damascenone. The sensory attributes showed scores higher than 7 on a 9-point hedonic scale for the FPB.25, illustrating that it was well accepted by panelists. Taken together, our results showed that PFJ could meet current consumer demand regarding natural and functional, fruit-based fermented beverages.