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Enzymological Evaluation of Bowel Inflammation Inhibitory Activity and Intestinal Microbial Flora Improvement by Enzymatic Hydrolysate of Schizandrae Fructus  

Ryu, Il-Hwan (College of Life Science and Natural Resources, Wonkwang University)
Kwon, Tae-Oh (College of Life Science and Natural Resources, Wonkwang University)
Lee, Kang-Soo (Facult of Biological Resources Science, Jeonbuk National University)
Yun, Yong-Gab (Department of Prescription, Wonkwang University School of Oriental Medicine)
Publication Information
Korean Journal of Pharmacognosy / v.38, no.4, 2007 , pp. 363-371 More about this Journal
Abstract
Schizandrae Fruits has been used as a traditional Oriental medicine for treatment of many stress-induced diseases. In the present study, we investigated inhibitory activity of enzymatic hydrolysate of Schizandrae fructus (SC-EX) in growth of tested intestinal microorganism and activity of bowel inflammation related enzyme. SC-EX was added to the proteose peptone-yeast extract-fildes (PYF) media to investigation the effect on the growth of type culture of intestinal microorganism. The growth of lactic acid bacteria such as Bifidobacterium species and Lactobacillus species was accelerated by more than 3% concentration of SC-EX. But, growth of harmfulness bacteria such as E.coli, Clostridium sp. Staphylococcus sp. Streptococcus sp. was inhibited by more than 3% concentration of SC-EX. Also, SC-EX was exhibited a concentration-dependent inhibitory activity of the bowel inflammation related enzymes. The SC-EX was showed 76% and 92% inhibitory activity of 5-lipoxygenase and cyclooygenase at 5% additional concentration respectively. Our results indicated that SC-EX may possess improvement effect on the intestinal flora and Anti-inflammatory effect on the bowel.
Keywords
Schizandrae fructus; intestinal microbial flora; enzymological evaluation;
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