Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Biological Effect and Chemical Composition Variation During Self-Fermentation of Stored Needle Extracts from Pinus densiflora Siebold & Zucc.

  • Paudyal, Dilli P. (Department of Biotechnology and BK21 Research Team for Protein Activity Control, Chosun University) ;
  • Park, Ga-Young (Department of Biotechnology and BK21 Research Team for Protein Activity Control, Chosun University) ;
  • Hwang, In-Deok (Department of Biotechnology and BK21 Research Team for Protein Activity Control, Chosun University) ;
  • Kim, Dong-Woon (Department of Clinical Pathology, Gwangyang Health College) ;
  • Cheong, Hyeon-Sook (Department of Biotechnology and BK21 Research Team for Protein Activity Control, Chosun University)
  • Published : 2007.12.31
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Abstract

Extract of Japanese red pine needles has been used in Asia pacific regions since long periods believing its valuable properties as tonic and ability of curing diseases of unidentified symptoms. Some selective compounds present in the extract and their effects were analyzed. Carbohydrates and vitamin c were identified using HPLC; terpenoid compounds by GC-MS; anti-bacterial analysis by paper discs, plates count and gastrointestinal motility by whole cell patch clamp. The extract is a mixture of compounds therefore its diverse effect was expected. Self-fermentation in extract proceeds after spontaneous appearance of yeast strains without inoculation. Effects and composition of the extract vary with varying period of self-fermentation. Extract inhibits the growth of bacteria dose dependently exhibiting its antibacterial properties however effectiveness increases with increase in fermentation period. The extract also can modulate gastrointestinal motility in murine small intestine by modulating pace maker currents in ICC mediated through ATP sensitive potassium channel.

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