• Journal of agricultural medicine and community health
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• v.15 no.1
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• pp.3-8
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• 1990

Seven specimens of salmon(Oncorhynchus keta) purchased directly, in Oct. 10. 1990, in the Taep'o port. Kang-won-do were examined for infection state of anisakid larvae and classification of extracted larvae according to morphological characters. The results were as follows. 1) From seven salmon 202 anisakid larvae were found, and mean infection number of anisakid larvae per individual salmon was 28.86. 2) From total extracted anisakid larvae 198 larvae(98%) were found in muscle. Therefore the distribution of anisakid larvae in intestine was extremely rare compared to in muscle. 3) The percentage of anisakid larvae in II, IV muscle region was 93% and from this we could surmise that most anisakid larvae in salmon did not penetrate to the very distanted muscle from intestine. 4) Three types of anisakid larvae(Anisakis Type I, Contracaecum Type B, Contracaecum Type D) were identified and, among them, Contracaecum Type B was the first recording type in Korea 5) Larvae of Contracaecum it genus were found only in intestine. Therefore it surmised that penetration neture to muscle of Contracaecum larvae was less than that of Anisakis Type I.

• Journal of agricultural medicine and community health
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• v.13 no.1
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• pp.32-40
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• 1988

Anisakiasis occur after the ingestion of raw marine fish and squid containg anisakid larvae. In recent years about 40 cases of anisakiasis have been recorded in Korea. Considering, however, the Korean style of eating raw fish, many more cases would exist and prevention is necessary. We describe the infection rate of anisakid larvae in 13 species of marine fish and squid which were caught in the Korean sea. And each extracted larva is classified according to morphological characters. The results are following ; Scomber japonicus, Pseudosciaena manchurica, Trichiurus haumela showed high infection rate of anisakid larvae. Although Sepia esculenta showed low infection rate, most of anisakid larvae found in Sepia edulis are embedded in muscles. So relative high frequent rate of anisakiasis may developed by Sepia esculenta. Five type (Anisakis Type I, Terranova Type B, Raphidascaris sp., Contracaecum Type A, Contracaecum Type D) of anisakid larvae are classified according to their morphological characters.

• Journal of agricultural medicine and community health
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• v.15 no.2
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• pp.89-96
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• 1990

The results of infection state of anisakid larvae and parasitic helminths in salmon and sea-trout which caught from Taep'o port. Kang-won do were as follows. 1) From twelve specimens of sea-trout. 122 individuals of anisakid larvae were found and mean infection number of anisakid larvae per individual sea-trout was 10.17. 2) From total extracted anisakid larvae from sea-trout. 37 larvae(30.3%) were found in the muscle. This percentage was lower than that of salmon. but higher than that of other sea-fishes. 3) The taxonomic list of parasitic helminths which found in salmon and sea-trout was as follows. Class Trematoda Family Hemiuridae l. Brachyphallus crenatus(Rudolphi. 1802) 2. Lecithaster salmonis Yamaguti. 1934 Class Cestoda Family Phllobothriidae 3. Pelichnibothrium speciosum Monticelli, 1889-larval form Family Tentacuariidae 4. Tentacularia sp. - larval form 4) Among those 4 species of helminths. B.crenatus. L.salmonis, P.speciosum were the first recording species in Korea.

• Journal of fish pathology
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• v.23 no.1
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• pp.123-129
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• 2010

The infestation status of anisakid type larvae was investigated in migrating chum salmon (Oncorhynchus keta), with different condition (captured area, sex, body portion) during 2006~2008. The mean infection number of anisakid larvae per individual female and male fish captured from Namdae river was $98{\pm}27$, $103{\pm}27$, respectively. The mean infection number of anisakid larvae per individual female and male fish captured from the coastal area of Yangyang was $63{\pm}18$ and $108{\pm}17$, respectively. The anisakid larvae were mainly found in abdominal muscles (85%) but only a little in the visceral portion. Two types of anisakid larvae (A. simplex, Contracaecum type) were identified but other anisakid larvae were not detected. To investigate the effect of storing temperature on the viability of anisakid larvae, the section of abdominal muscle were stored at different temperature (room temperature, $4^{\circ}C$, $-20^{\circ}C$, $-80^{\circ}C$). As a result, it was necessary to store at $-20^{\circ}C$ for more than 6 hrs to kill the larvae. The present results revealed that chum salmon caught in Korea are heavily infected with anisakid larvae, mainly in the abdominal muscle, and A. simplex was dominantly found in this study.

• Journal of Environmental Health Sciences
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• v.26 no.2
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• pp.67-69
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• 2000

Yellow tails (Seriola quinqueradiata) were purchased from the Jakalchi fish market located in Busna and examined for anisakid larvae. The collected larvae were classified by their morphologicaltypes. Of the 16 yellow tails exmained, seven (43.8%) were infected with the larvae. Among 90 larvae, 81 were collected from the omentum and 9 from the muscle. Anisakids were classified into five larval types, anisakis type I (56 larvae in number, 62.2%), Contracaecum type A (8, 8.9%) and type D (7, 7.8%) and Contracaecum type C (19, 21.1%). The present study revealed that Anisakis type I was the most common among the five larval types in the yellow tails caught in the South sea of Korea peninsula.

• Journal of Environmental Health Sciences
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• v.23 no.1
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• pp.14-17
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• 1997

A total of 93 specimens of Astroconger myriaster were purchased from the Chungmu fishery market at the south sea area during the period from October 1 to November 25, 1996. Specimens were examined for the infection status with larvae anisakid. Anisakid larvae were collected from viscera, muscle, omenturn and liver. Eight hundred four larval anisakids sorted from 93 specimens of A. myriaster ranged from 9.0 to 26.5mm in their body length. They were classified based on morphological and morphometric observations as follows Anisakis type 1 larvae (465 larvae, 57.8%: positive rate), Contracaecum type D(129, 16.0%), Contracaecum type A(90, 11.2%), Anisakis type 11(60, 7.5%), Phapidascaris(3, 0.4%) and unknown type(57, 7.1%).

• Parasites, Hosts and Diseases
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• v.50 no.4
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• pp.295-299
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• 2012

The present study was performed to determine the infection status of anisakid larvae in marine fish collected from 3 sea areas of the Republic of Korea. Total 86 marine fish (8 species) collected from the East Sea (Goseong-gun, Gangwon-do), 171 fish (10 species) from the South Sea (Sacheon-si, Gyeongsangnam-do), and 92 fish (7 species) from the Yellow Sea (Incheon Metropolitan City) were examined by both naked eyes and artificial digestion method. Among the total of 349 fish examined, 213 (61.0%) were infected with 8 species of anisakid larvae, i.e., Anisakis simplex, 6 types of Contracaecum spp., and Raphidascaris sp., and the mean larval density was 13.8 per infected fish. Anisakid larvae were detected in 45 fish (52.3%) from the East Sea, 131 fish (76.6%) from the South Sea, and 37 fish (40.2%) from the Yellow Sea. The average numbers of larvae detected were 4.0, 16.6, and 15.9, respectively. Anisakis simplex larvae were detected in 149 fish (42.7%), and the mean larval density was 9.0 per infected fish. They were found in 26 fish (30.2%) collected from the East Sea, 96 fish (56.1%) from the South Sea, and 27 fish (29.3%) from the Yellow Sea. The average numbers of larvae detected were 2.9, 10.3, and 10.5, respectively. Conclusively, the present study suggests that the infection rate and density of anisakid larvae are more or less higher in the fish from the South Sea than those from the East Sea or the Yellow Sea.

• Parasites, Hosts and Diseases
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• v.57 no.6
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• pp.699-704
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• 2019

Anisakiasis (anisakidosis) refers to a foodborne zoonosis caused by ingesting raw or undercooked marine fish or cephalopods infected with anisakid larvae. The present study was performed to investigate the prevalence of anisakid larvae in anchovies (Engraulis japonica) purchased from 2 local markets in Gyeongsangnam-do, the Republic of Korea (=Korea), during 2018-2019. Anchovies were transported to our laboratory and examined by pepsin-HCl artificial digestion technique followed by microscopic observations and molecular analyses. The overall prevalence of anisakid larvae was 19.5% (39/200), from which a total of 51 larvae (av. 1.3 larvae/infected anchovy) were recovered. Sequencing of the larvae targeting the ITS region, including ITS1, 5.8S rRNA, and ITS2 genes confirmed the species of larvae as Anisakis pegreffii (54.9%; 28/51), Hysterothylacium sinense (23.5%; 12/51), and Hysterothylacium aduncum (21.5%; 11/51). The results suggested that anchovies could be a potential source of human anisakiasis in Korea.

• Journal of fish pathology
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• v.23 no.3
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• pp.421-427
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• 2010

Anisakid nematodes third stage larvae were isolated from the muscles of masou salmon (Oncorhynchus masou). Fish were purchased from Jumunjin fishery market in Gangneung. Four Anisakid third stage larvae were isolated from 4 fish. Molecular identification of the isolated worms was conducted by PCR-RFLP analysis of ribosomal DNA internal transcribed spacer region and direct sequencing of mitochondrial DNA cox2 gene. As results, all the tested individual worms were identified as Anisakis simplex (sensu stricto). This is the first report of molecular detection of anisakid worms in salmonid fishes in Korea.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.1 no.1
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• pp.55-57
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• 1996

A total of 248 specimens of Todarodes pacificus were purchased from the Jumungin fishery market at the East Sea area two times during the period from November 1 to December 30, 1995. Samples were examined for their infection status with larvae anisakid. Anisakid larvae were collected from muscle, viscera and omentum. One hundred sixteen larval anisakids sorted from 34 specimens of T. pacificus (13.7% of infection rate) ranged from 13.5 to 29.5 mm in their body length. They were classified based on morphological and morphometric observations as follows; Anisakis type I larvae (68 larvae, 58.6%: positive rate), Contracaecum type A (5, 4.3%), Anisakis type II (17, 14.5%), Contracaecum type D (8, 7.0%) and unknown type (18, 15.5%).