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Molecular identification of Anisakid worm third stage larvae isolated from masou salmon Oncorhynchus masou  

Jeon, Chan-Hyeok (Faculty of Marine Bioscience and Technology, Gangneung-Wonju National University)
Setyobudi, Eko (Faculty of Marine Bioscience and Technology, Gangneung-Wonju National University)
Kim, Jeong-Ho (Faculty of Marine Bioscience and Technology, Gangneung-Wonju National University)
Publication Information
Journal of fish pathology / v.23, no.3, 2010 , pp. 421-427 More about this Journal
Abstract
Anisakid nematodes third stage larvae were isolated from the muscles of masou salmon (Oncorhynchus masou). Fish were purchased from Jumunjin fishery market in Gangneung. Four Anisakid third stage larvae were isolated from 4 fish. Molecular identification of the isolated worms was conducted by PCR-RFLP analysis of ribosomal DNA internal transcribed spacer region and direct sequencing of mitochondrial DNA cox2 gene. As results, all the tested individual worms were identified as Anisakis simplex (sensu stricto). This is the first report of molecular detection of anisakid worms in salmonid fishes in Korea.
Keywords
Anisakis simplex (s.s.); Oncorhynchus masou; Masu salmon; PCR-RFLP; Mitochondrial cox2 gene;
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