Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Molecular identification of Anisakid worm third stage larvae isolated from masou salmon Oncorhynchus masou

시마연어, Oncorhynchus masou에서 분리된 아니사키스 속 선충 3기 유충의 분자생물학적 방법을 이용한 동정

  • Jeon, Chan-Hyeok (Faculty of Marine Bioscience and Technology, Gangneung-Wonju National University) ;
  • Setyobudi, Eko (Faculty of Marine Bioscience and Technology, Gangneung-Wonju National University) ;
  • Kim, Jeong-Ho (Faculty of Marine Bioscience and Technology, Gangneung-Wonju National University)
  • 전찬혁 (강릉원주대학교 생명과학대학 해양생명공학부 해양자원육성) ;
  • ;
  • 김정호 (강릉원주대학교 생명과학대학 해양생명공학부 해양자원육성)
  • Received : 2010.08.11
  • Accepted : 2010.10.28
  • Published : 2010.12.31
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Abstract

Anisakid nematodes third stage larvae were isolated from the muscles of masou salmon (Oncorhynchus masou). Fish were purchased from Jumunjin fishery market in Gangneung. Four Anisakid third stage larvae were isolated from 4 fish. Molecular identification of the isolated worms was conducted by PCR-RFLP analysis of ribosomal DNA internal transcribed spacer region and direct sequencing of mitochondrial DNA cox2 gene. As results, all the tested individual worms were identified as Anisakis simplex (sensu stricto). This is the first report of molecular detection of anisakid worms in salmonid fishes in Korea.

Keywords

References

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