• Journal of radiological science and technology
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• v.33 no.3
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• pp.203-211
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• 2010

The purpose of this study is meant to develop an evaluation framework for PBL and S-PBL package, as well as verify reliability and propriety of developed framework. To achieve the purpose, existing researches and related documents were considered, and composed basic questionnaire which fits to the basic frame of the framework, 3 categories 15 items for PBL/S-PBL. Verifying its contents propriety, examined by 9 experts who professors in college and made 14 items for PBL, 15 items for S-PBL. To verify construct validity of basic frameworks, carried out factor analysis. Final evaluation framework questionnaire were 15 items selected, its PBL for 3 items in motivating, 7 items in integrity, 4 items in systematic and total 14 items, S-PBL for 4 items in motivating, 6 items in integrity, 5 items in systematic and total 15 items, and was verify to have adequate reliability with Cronbach's $\alpha$ is 0.9112 in PBL, 0.9166 of S-PBL.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.2
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• pp.651-656
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• 2014

Background: Cervical cancer is the third commonest type of cancer among women in Malaysia. Our aim was to determine the distribution of human papilloma virus (HPV) genotypes in cervical cancer in our multi-ethnic population. Materials and Methods: This was a multicentre study with a total of 280 cases of cervical cancer from 4 referral centres in Malaysia, studied using real-time polymerase chain reaction (qPCR) detection of 12 high risk-HPV genotypes. Results: Overall HPV was detected in 92.5% of cases, in 95.9% of squamous cell carcinomas and 84.3%of adenocarcinomas. The five most prevalent high-risk HPV genotypes were HPV 16 (68.2%), 18 (40%), 58 (10.7%), 33 (10.4%) and 52 (10.4%). Multiple HPV infections were more prevalent (55.7%) than single HPV infections (36.8%). The percentage of HPV positive cases in Chinese, Malays and Indians were 95.5%, 91.9% and 80.0%, respectively. HPV 16 and 18 genotypes were the commonest in all ethnic groups. We found that the percentage of HPV 16 infection was significantly higher in Chinese (75.9%) compared to Malays (63.7%) and Indians (52.0%) (p<0.05), while HPV 18 was significantly higher in Malays (52.6%) compared to Chinese (25.0%) and Indians (28%) (p<0.05). Meanwhile, HPV 33 (17.9%) and 52 (15.2%) were also more commonly detected in the Chinese (p<0.05). Conclusions: This study showed that the distribution of HPV genotype in Malaysia is similar to other Asian countries. Importantly, we found that different ethnic groups in Malaysia have different HPV genotype infection rates, which is a point to consider during the implementation of HPV vaccination.

• 한국노년학
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• v.36 no.3
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• pp.765-783
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• 2016

This study examines 2014 National Survey on Older Koreans (10,279 persons aged 65 or above) to understand social networks among older Koreans. In order to classify the types of social relationships, the latent profile analysis is conducted based on such structural aspects of social networks as network size, frequencies of contact, and closeness. The results show that older Koreans can be categorized into 5 distinct social network types: disengaged (11.6%), ritual-family-focused (17.7%), close-family-focused (23.6%), close-restricted (28,4%), and diverse (18.8%). Characteristics by each social network type are compared and multinominal logistic regression analyses are applied to figure out the influencing factors of social network type. Older persons with disengaged social network ties tend to be overwhelmingly female, living alone, old-old, and of low socioeconomic status. On the other hand, older persons with diverse social network ties tend to be young-old and had highly-ranked jobs in their mid-life. Spouse/children are the focal point of social relationships in both ritual-family-focused and close-restricted social network. However, the proportion of men is higher in the close-family-focused type than in the ritual-family-focused. Older Koreans with close-restricted social network ties tend to be female, uneducated, and have engaged in agricultural and allied activities. This study discusses the implications of its findings and how research in this area should develop in the future.

• Journal of Microbiology and Biotechnology
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• v.26 no.10
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• pp.1817-1823
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• 2016

Areca nut (AN) chewing is a habit in many countries in Central, Southern, and Southeast Asia. It is strongly associated with the occurrence of oral, pharyngeal, and esophageal cancer as well as systemic inflammation. However, the association between AN intake and the development of gastric lesions has not yet been identified. The aim of this study was to investigate the effect of AN on gastric diseases using a mouse model for Helicobacter pylori infection. We studied four groups of mice: those fed a normal diet (ND), those fed a diet containing 2.5% AN (AD), those fed ND and infected with H. pylori PMSS1 strain (ND/HP), and those fed AD and infected with H. pylori PMSS1 strain (AD/HP). Food intake and body weight were monitored weekly during the experiments. At 10 weeks, the mice were sacrificed, and the stomach weight, H. pylori colonization, and gastric inflammation were evaluated. The stomach weight had increased significantly in the ND/HP and AD/HP groups along with increases in H. pylori colonization; however, there was no significant difference between these two groups with respect to stomach weight and colonization. On histological grading, mononuclear cell infiltration was severer in the AD/HP group than in the ND/HP group. These data suggest that chronic gastric inflammation was aggravated by AN treatment in the mice with H. pylori-induced gastric lesions. Furthermore, as previously suggested, this animal model is useful to determine the effect of potential carcinogens on gastric lesions induced by H. pylori infection.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.12 no.1
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• pp.281-287
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• 2011

In anatomical education, cyber class has been studied as a strong strategy coincided with academic characterestics. In the present study, we investigated the correlation between self-evaluation factors, use of cyber classroom and academic achievement of health allied undergraduate students according to application of cyber class in the anatomical education. The subjects of study were totally 231 students who were majored in nursing science (n=143) and sports science (n=88) in last 3 years (2007-2009). Based on frequency of log-in, survey and grade of score, we analyzed correlation of their factors and their scores using frequency analysis, T-test and analysis of variance. Regardless of their major, most of students gave high scores in all of self-evaluation factors, convinience of cyber classroom, suitability of assignment. The frequency of log-in correlated highly with academic achievement(r=0.246, p<0.01, nursing science; r=0.234, p<0.05 sports medicine). The academic achievement also had a high correlation of 0.218(p<0.01) with the level of difficulty in nursing science students, 0.499(p<0.05) with the levels of satisfaction difficulty and interest. in sports science students. This study suggested that cyber class was very useful and would be a promising strategy in anatomical education.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.7
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• pp.2929-2937
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• 2015

Endothelial nitric oxide synthase (eNOS or NOS3) produces nitric oxide and genetic polymorphisms of NOS3 gene play significant roles in various processes of carcinogenesis. The results from published studies on the association between NOS3 G894T and NOS3 intron 4 (4a/b) polymorphisms and cancer risk are conflicting and inconclusive. However, i n order to assess this relationship more precisely, a meta-analysis was performed with PubMed (Medline), EMBASE and Google web searches until February 2014 to select all published case-control and cohort studies. Genotype distribution data were collected to calculate the pooled odd ratios (ORs) and 95% confidence intervals (CIs) to evaluate the strength of association. A total of 10,546 cancer cases and 10,550 controls were included from twenty four case-control studies for the NOS3 G894T polymorphism. The results indicated no significant association with cancer risk as observed in allelic (T vs G: OR=1.024, 95%CI=0.954 to 1.099, p=0.508), homozygous (TT vs GG: OR=1.137, 95%CI=0.944 to 1.370, p=0.176), heterozygous (GT vs GG: OR=0.993, 95%CI=0.932 to 1.059, p=0.835), recessive (TT vs GG+GT: OR=1.100, 95%CI=0.936 to 1.293, p=0.249) and dominant (TT+GT vs GG: OR=1.012, 95%CI=0.927 to 1.105, p=0.789) genetic models. Similarly, a total of 3,449 cancer cases and 3,691 controls were recruited from fourteen case-control studies for NOS3 4a/b polymorphism. Pooled results indicated no significant association under allelic (A vs B: OR=0.981, 95%CI=0.725 to 1.329, p=0.902), homozygous (AA vs BB: OR=1.166, 95%CI=0.524 to 2.593, p=0.707), heterozygous (BA vs BB: OR=1.129, 95%CI=0.896 to 1.422, p=0.305), dominant (AA+BA vs BB: OR=1.046, 95%CI=0.779 to 1.405, p=0.763) and recessive (AA vs BB+BA: OR=1.196, 95%CI=0.587 to 2.439, p=0.622) genetic contrast models. This meta-analysis suggests that G894T and 4a/b polymorphisms of NOS3 gene are not associated with increased or decreased risk of overall cancer.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.8
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• pp.4003-4007
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• 2016

Long interspersed elements-1s (LINE-1s) are dispersed all over the human genome. There is evidence that hypomethylation of LINE-1s and levels of sex steroids regulate gene expression leading to cancer development. Here, we compared mRNA levels of genes containing an intragenic LINE-1 in breast cancer cells treated with various sex steroids from Gene Expression Omnibus (GEO), with the gene expression database using chi-square analysis (http://www.ncbi.nlm.nih.gov/geo). We evaluated whether sex steroids influence expression of genes containing an intragenic LINE-1. Three sex steroids at various concentrations, 1 and 10 nM estradiol (E2), 10 nM progesterone (PG) and 10 nM androgen (AN), were assessed. In breast cancer cells treated with 1 or 10 nM E2, a significant percentage of genes containing an intragenic LINE-1 were down-regulated. A highly significant percentage of E2-regulated genes containing an intragenic LINE-1 was down-regulated in cells treated with 1 nM E2 for 3 hours (p<3.70E-25; OR=1.91; 95% CI=2.16-1.69). Similarly, high percentages of PG or AN-regulated genes containing an intragenic LINE-1 wwere also down-regulated in cells treated with 10 nM PG or 10 nM AN for 16 hr (p=9.53E-06; OR=1.65; 95% CI=2.06-1.32 and p=3.81E-14; OR=2.01; 95% CI=2.42-1.67). Interestingly, a significant percentage of AN-regulated genes containing an intragenic LINE-1 was up-regulated in cells treated with 10 nM AN for 16 hr (p=4.03E-02; OR=1.40; 95% CI=1.95-1.01). These findings suggest that intragenic LINE-1s may play roles in sex steroid mediated gene expression in breast cancer cells, which could have significant implications for the development and progression of sex steroid-dependent cancers.

• The Korean Journal of Food And Nutrition
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• v.16 no.2
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• pp.158-164
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• 2003

This study was conducted to investigate the properties of noodle which was made of composite flour blended with the powder of synnemata of Beauveria bassiana. The characteristics of cooked-noodle including color, cooking properties, mechanical texture properties were measured, and sensory evaluation was performed. The L-value of dry and wet-noodle which made of composite flour was similar to that of 100% wheat flour, but a and b-value were higher than those of 100% wheat flour. There were no differences in the weight, volume and water absorption of the cooked-noodle made of composite flour and that of 100% wheat flour, but the turbidity of the cooked-noodle made of composite flour was higher than that of 100% wheat flour. The breaking force of dry-noodle which was made of composite flour was same as that of 100% wheat flour. Hardness and Gumminess of the cooked-noodle which was made of composite flour blended with synnemata powder at level of 1∼5% were same as those of 100% wheat flour. Springiness, chewiness, cohesiveness and adhesiveness of the cooked-noodle which was made of composite flour blended with synnemata powder at level of 1% were similar to those 100% wheat flour. The results of sensory evaluation showed that the cooked-noodles containing 1% and 3% synnemata powder were acceptable as much as those of 100% wheat flour in terms of color, taste, flavor, texture and overall acceptance.

• Korean Journal of Food Science and Technology
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• v.30 no.1
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• pp.139-145
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• 1998

This study was performed to provide fundamental data on the optimum manufacture condition of oleoresin using citron peel. Oleoresin was extracted from freeze-dried or hot air dried citron peels using various solvents (hexane, ether, dichloromethane, acetone. and methanol), mixing ratio, extraction temperature, and time. As a result, optimum extraction conditions of oleoresin were: solvent mixing ratio 1:10 (w/v), extraction time 2 hours, and extraction temperature $60^{\circ}C$ when used methanol, and their dichloromethane 1:10 (w/v), 4 hours and $20^{\circ}C$, respectively. At optimum extraction conditions, the yield of oleoresin was shown that 35.79% at hot air drying samples, 32.04% at freeze-dried ones when extracted by methanol, but shown 5.86% and 6.16% when used dichloromethane respectively. The number of volatile components present in citron oleoresin were confirmed as thirty two in methnol extracion method and twenty nine in dichloromethane extraction method by GC and GC/MS, respectively. But, in the kinds and amounts of volatile flavor components, relatively greater numbers of volatiles were identified in freeze-dried sample extracted by dichloromethane compared with other methods. In freeze-dried sample extracted by dichloromethane, volatile components of citron oleoresin predominantly occupied by limonene and ${\gamma}-terpinene$ with about 85%. Other important compounds were shown hydrocarbons. such as ${\alpha}-pinene$, myrcene, terpinolene, ${\beta}-farnesene\;and,\;{\delta}-elemene$, and linalool as alcohols.

• Journal of Ginseng Research
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• v.28 no.1
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• pp.39-44
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• 2004

The present study is to determine whether the Red-ginseng extract has a proliferative or cytotoxic effect on the human neuronal stem cells(hNSCs). The hNSCs were grown and incubated with different doses of Red-ginseng extract. We tested the proliferative or cytotoxic effects by MTT and FACS analysis. Cell viability cell cycle analysis, DNA fragmentation, and bax or PARP expressions were evaluated. The hNSCs showed a proliferafe trend with its peak concentration at 0.3 $\mu\textrm{g}$/$m\ell$. Beyond this point, higher doses decreased viabilities and showed a cytotoxic effect at 10 $\mu\textrm{g}$/$m\ell$. There was a tendency of increased S and G2/M phases during cell proliferation. In a cytotoxic condition, decreased S phase and increased G0/G1 phases were noted, suggesting cell cycle arrest. The cytotoxic effect was associated with increase DNA fragmentation in a dose-dependent manner, However PARP cleavage or bax expression was not detected. Our results suggest that Red-ginseng extract has dual effects, the cell proliferative or cytotoxic effect, on hNSCs in vitro with dose-dependent manner.