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http://dx.doi.org/10.5142/JGR.2004.28.1.039

The Red-ginseng Extract Alters the Cell Cycle and Viability in the Human Neuronal Stem Cells  

Kim, Hyun-Jung (Department of Neurology, Seoul National University Hospital)
Kang, La-Mi (Department of Neurology, Seoul National University Hospital)
Ahn, Jin-Young (Department of Neurology, Gangnam General Hospital Public Corporation)
Han, Jung-Soon (Department of Nutrition and Food Sciences of Junior College of Allied Health Sciences of Korea University/Diet Research Institute of InJae University)
Kim, Seung-U. (Brain Disease Research Center, Ajou University)
Lee, Kwang-Woo (Department of Neurology, Seoul National University Hospital)
Kim, Man-Ho (Department of Neurology, Seoul National University Hospital)
Publication Information
Journal of Ginseng Research / v.28, no.1, 2004 , pp. 39-44 More about this Journal
Abstract
The present study is to determine whether the Red-ginseng extract has a proliferative or cytotoxic effect on the human neuronal stem cells(hNSCs). The hNSCs were grown and incubated with different doses of Red-ginseng extract. We tested the proliferative or cytotoxic effects by MTT and FACS analysis. Cell viability cell cycle analysis, DNA fragmentation, and bax or PARP expressions were evaluated. The hNSCs showed a proliferafe trend with its peak concentration at 0.3 $\mu\textrm{g}$/$m\ell$. Beyond this point, higher doses decreased viabilities and showed a cytotoxic effect at 10 $\mu\textrm{g}$/$m\ell$. There was a tendency of increased S and G2/M phases during cell proliferation. In a cytotoxic condition, decreased S phase and increased G0/G1 phases were noted, suggesting cell cycle arrest. The cytotoxic effect was associated with increase DNA fragmentation in a dose-dependent manner, However PARP cleavage or bax expression was not detected. Our results suggest that Red-ginseng extract has dual effects, the cell proliferative or cytotoxic effect, on hNSCs in vitro with dose-dependent manner.
Keywords
Red ginseng; human neuronal stem cell; cell proliferation; cell cycle; cytotoxicity;
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