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Improvements in Storage Stability of Undaria pinnatifida after Gamma Irradiation (감마선 조사에 의한 미역의 저장성 개선)

  • Kim, Hyun-Joo;Choi, Jong-Il;Kim, Jae-Hun;Chun, Byung-Soo;Ahn, Dong-Hyun;Kim, Gwang-Hoon;Byun, Myung-Woo;Lee, Ju-Woon
    • Food Science and Preservation
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    • v.16 no.4
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    • pp.590-593
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    • 2009
  • The effect of gamma irradiation on the storage stability of Undaria pinnatifida was investigated. A three-log reduction in total aerobic bacteria in U. pinnatifida samples was obtained by irradiation with 3 kGy. During storage for 2 weeks, the viable counts of microorganisms in non-irradiated U. pinnatifida increased to 6 log CFU/g. However, irradiation of U. pinnatifida by 5 kGy eliminated microorganisms. The Hunter $L^{\ast}-value$ of U. pinnatifida was slightly increased after 5 kGy of irradiation. The hardness properties of U. pinnatifida decreased during storage, but were not changed by irradiation with up to 3 kGy. These results indicate that low-dose irradiation (3 kGy) could be effective for improvement of the storage stability of U. pinnatifida.

Effects of Xylanase on Performance, Blood Parameters, Intestinal Morphology, Microflora and Digestive Enzyme Activities of Broilers Fed Wheat-based Diets

  • Luo, Dingyuan;Yang, Fengxia;Yang, Xiaojun;Yao, Junhu;Shi, Baojun;Zhou, Zhenfeng
    • Asian-Australasian Journal of Animal Sciences
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    • v.22 no.9
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    • pp.1288-1295
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    • 2009
  • The study was conducted to investigate the effects of different levels of xylanase on performance, blood parameters, intestinal morphology, microflora and digestive enzyme activities of broilers. The wheat-based diets were supplemented with 0, 500, 1,000, 5,000 U/kg xylanase. Xylanase supplementation significantly (p<0.05) improved the feed:gain ratio of broilers from 1 to 21 d and 1 to 42 d. Supplementing 500 U/kg and 1,000 U/kg xylanase improved (p<0.05) the villus height and the ratio of villus height to crypt depth in the small intestine. Excess supplementation of xylanase (5,000 U/kg) increased the villus height in the ileum (p<0.01) and the ratio of villus height to crypt depth in the duodenum and ileum (p<0.05). The microflora in the ileum and caecum, digestive enzyme activities in the small intestine and the concentrations of serum glucose, uric acid, insulin and IGF-I were not affected by the supplementation of xylanase. Excess level of xylanase (5,000 U/kg) had a tendency to induce the multiplication of E. coli and total aerobes. The results suggested that supplementing 500 U/kg and 1,000 U/kg xylanase was beneficial for broilers and excess xylanase supplementation resulted in no further improvement or negative effects.

Role of Plasma Osmolality in AVP and Aldosterone Release in Korean Young Men (혈장 삼투질 농도 변동에 따른 항이뇨 호르몬과 Aldosterone 분비조절 : 한국청년)

  • Lee, Won-Jung;Choo, Young-Eun;Koo, Ja-Hoon
    • The Korean Journal of Physiology
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    • v.21 no.2
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    • pp.297-304
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    • 1987
  • A study was carried out to find out the relationship between arginine vasopressin (AVP) release and plasma osmolality in 15 young men (age: 21.4 yr). After an overnight fasting, wale. (20 ml/kg) was imbibed, and venous blood and urine samples were collected every 30 min for 90 min. then 5% saline was infused (0.06 ml/min/kg) for 120 min. AVP was extracted on Sep-Pak column and measured by radioimmunoassay. Under basal condition, plasma osmolality (pOsm), AVP (pAVP) and aldosterone (pAldo) levels were 286.5 mOsm/kg, 1.1 pg/ml, and 140 pg/ml, respectively. pAVP became undetectable during maximum water diuresis, and increased in response to hypertonic saline infusion. pAVP level began to increase when pOsm was above 280 mOsm/kg. Changes in urinary AVP excretion (uAVP) was parallel to pAVP levels. The fall in pAVP was followed by a decrease in uAVP, uOsm and an increase in free water clearance, while the later rise in pAVP was followed by an increase in uAVP, uOsm and a decrease in free water clearance. When pooling all data together, relationships between pAVP and pOsm, and uAVP and uOsm were best expressed by an exponential relationship (r=0.78, 0.86, respectively). pAldo level decreased to 71 pg/ml after water ingestion, and decreased further to 30 pg/ml 2 hr after 5% saline infusion. Even at the same pNa, pAldo level during dehydration state was significantly higher than during hydration state. Negative exponential relation (r=-0.59) was observed between pAldo and pNa. Response to change in body fluid volume was greater in aldosterone than in AVP release.

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Inhibitory effects of Sanghwang mushrooms, Phellinus linteus strains and Phellinus baumii, on platelet aggregation (상황버섯, Phellinus linteus 계통과 Phellinus baumii의 항 혈소판 응집 효과)

  • Kang, Hee-Wan;Rhee, Man-Hee;Yeo, Woon-Hyung
    • Journal of Mushroom
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    • v.15 no.3
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    • pp.124-128
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    • 2017
  • The fruiting bodies of Sanghwang mushrooms, Phellinus linteus HN1009K (PLH) and Phellinus linteus (Korea Sanghwang, PLK), and Phellinus baumii (Jangsu Sangwhang, PB), were extracted with 70% methanol. The methanolic extracts of different concentrations ($5-200{\mu}g/ml$) showed inhibitory effects of 20-95% on plated aggregation induced by collagen (2.5 ug/ml), ADP (10 uM), and thrombin (0.1 U/ml). The PLH, PLK, and PB extracts (200 ug/ml) reduced ATP release from ADP-activated platelets by 50-60%. These results suggest that the methanolic extracts from Sanghwang mushrooms inhibit platelet aggregation.

A Multi-Band Antenna on Automobile-Glass Using Flexible PCB (유연성 기판을 이용한 자동차 유리 부착용 다중 대역 안테나)

  • Kim, In-Bok;Woo, Dong-Sik;Kim, Kang Wook
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.24 no.1
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    • pp.20-26
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    • 2013
  • In this paper, we propose a multi-band monopole antenna with a band-notching U-slot, which is fabricated inside the flexible polymide film substrate. The U-shaped slot located on the patch-shaped monopole antenna provides band-notch at 2.7 GHz, but also helps to improve return loss at adjacent frequency bands. The performance of the antenna attached on an automobile-glass has been simulated and measured. The fabricated antenna provides more than 10 dB return loss for ISM band(2.4~2.483 GHz) and WAVE band(5.85~5.925 GHz), 2.8~5.7 dBi maximum gain, and good radiation patterns.

A 28-GHz Wideband 2×2 U-Slot Patch Array Antenna

  • Yoon, Nanae;Seo, Chulhun
    • Journal of electromagnetic engineering and science
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    • v.17 no.3
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    • pp.133-137
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    • 2017
  • In this study, a 28-GHz U-slot array antenna for a wideband communication system is proposed. The U-slot patch antenna structure consists of a patch, two U-shaped slot, and a ground plane. With the additional U-slot, the proposed antenna has around 10% of bandwidth at -10 dB. To increase gain, the U-slot antenna is arrayed to $2{\times}2$. The proposed antenna is designed and fabricated. The $2{\times}2$ array antenna volume is $41.3mm{\times}46mm{\times}0.508mm$. The proposed antenna was measured and compared with the simulation results to prove the reliability of the design. The bandwidth and gain of the measurement results are 3.35 GHz and 13 dBi, respectively and the operating frequency is around 28 GHz.

The SCORM Based Learning Support Framework for Ubiquitous Environment (유비쿼터스 환경을 위한 SCORM 기반의 학습지원 프레임워크)

  • Jeong, Hwa-Young;Hong, Bong-Hwa
    • Journal of Advanced Navigation Technology
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    • v.14 no.5
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    • pp.661-667
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    • 2010
  • A lot of existence e-learning are connected SCORM and LMS. And u-learning was researching as one of the new trend. But there are few research paper to connect the existing SCORM and LMS. In this paper, we proposed u-learning framework with connect the SCORM and LMS. And we used the mobile equipment transform module and learning object reconstruction module to apply each different characteristics of mobile equipment. Especially, information of the mobile equipment was stored and managed using the meta-data of the equipment.

INFINITELY MANY SMALL SOLUTIONS FOR THE p&q-LAPLACIAN PROBLEM WITH CRITICAL SOBOLEV AND HARDY EXPONENTS

  • Liang, Sihua;Zhang, Jihui;Fan, Fan
    • Journal of applied mathematics & informatics
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    • v.28 no.5_6
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    • pp.1143-1156
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    • 2010
  • In this paper, we study the following p&q-Laplacian problem with critical Sobolev and Hardy exponents {$-{\Delta}_pu-{\Delta}_qu={\mu}\frac{{\mid}u{\mid}^{p^*(s)-2}u}{{\mid}x{\mid}^s}+{\lambda}f(x,\;u)$, in $\Omega$, u=0, on $\Omega$, where ${\Omega}\;{\subset}\;\mathbb{R}^{\mathbb{N}}$ is a bounded domain and ${\Delta}_ru=div({\mid}{\nabla}u{\mid}^{r-2}{\nabla}u)$ is the r-Laplacian of u. By using the variational method and concentration-compactness principle, we obtain the existence of infinitely many small solutions for above problem which are the complement of previously known results.

Analysis of 5-aza-2'-deoxycytidine-induced Gene Expression in Lung Cancer Cell Lines (폐암 세포주에서 5-aza-2'-deoxycytidine 처치에 의해 발현되는 암항원 유전자 분석)

  • 김창수;이해영;김종인;장희경;박종욱;조성래
    • Journal of Chest Surgery
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    • v.37 no.12
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    • pp.967-977
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    • 2004
  • Background: DNA methylation is one of the important gene expression mechanisms of the cell. When cytosine of CpG dinucleotide in promotor is hypomethylated, expression of some genes that is controlled by this promoter is altered. In this study, the author investigated the effect of DNA demethylating agent, 5-aza-2'-deoxycytidine (ADC), on the expressions of cancer antigen genes, MHC and B7 in 4 lung cancer cell lines, NCIH1703, NCIH522, MRC-5, and A549. Material and Method: After treatment of cell lines, NCIH1703, NCIH522, MRC-5 and A549 with ADC (1 uM) for 48 hours, RT-PCR was performed by using the primers of MAGE, GAGE, NY-ESO-1, PSMA, CEA, and SCC antigen gene. In order to find the optimal ADC treatment condition for induction of cancer antigen, we studied the effect of ADC treatment time and dose on the cancer antigen gene expression. To know the effect of ADC on the expression of MHC or B7 and cell growth, cells were treated with 1 uM of ADC for 72 hours for FACS analysis or cells were treated with 0.2, 1 or 5 uM of ADC for 96 hours for cell counting. Result: After treatment of ADC (1 uM) for 48 hours, the expressions of MAGE, GAGE, NY-ESO-1, and PSMA genes increased in some cell lines. Among 6 MAGE isotypes tested, and gene expression of MAGE-1, -2, -3, -4 and -6 could be induced by ADC treatment. However, CEA gene expression did not change and SCC gene expression was decreased by ADC treatment. Gene expression was generally induced 24 - 28 hours after ADC treatment and expression of MAGE, GAGE, and NY-ESO-1 was maintained at least 14 days after ADC ADC teatment, and expression of MAGE, GAGE, and NY-ESO-1 was maintained at least 14 days after ADC teatment in ADC-Free medium. Most gene expression could be induced at 0.2 uM of ADC, but gene expression increased dependently on ADC treatment dose. The expression of MHC and B7 was not increased by ADC treatment in all four cell lines, and the growth rate of 4 cell lines decreased significantly with the increase of ADC concentrations. Conclusion: Treatment of lung cancer cell lines with ADC increases the gene expression MAGE, GAGE and NY-ESO-1 that are capable of induction of cytotoxic T lymphocyte response. We suggest that treatment with 1 uM of ADC for 48 hours and then culturing in ADC-free medium is optimal condition for induction of cancer antigen. However, ADC has no effect on MHC and B7 induction, additional modification for increase of expression of MHC, B7 and cytokine will be needed for production of efficient cancer cell vaccine.

Analysis on Connection of Information Infra for Efficient Ubiquitous-service Realization (효율적인 u-서비스 구현을 위한 정보 인프라 연계성 분석)

  • Choi, Pill-Soon;Kang, Joon-Mook;Park, Joon-Kyu
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.29 no.5
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    • pp.497-507
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    • 2011
  • Ubiquitous city which was appeared since 2006 is defined as "The city provided the ubiquitous city service anytime and anywhere using ubiquitous urban infrastructure to improve the city's competitiveness and quality of life" under ubiquitous city construction law. To realize the ubiquitous city, the city has to be constructed efficiently based on the spatial information. And connection of information achieve effective synergy. In this study, applied multilateral analysis to spatial information and administrative information to implement u-service focused on MACCA(Multifunctional Administrative City Construction Agency). Study about main function and product data with connection process of Korean Land Spatialization System and administrative information System was performed. Also, details of u-service and method of acquiring necessary data were derived. In Addition, the information connection possibility for efficient u-service realization was suggested by linkage analysis method to utilize the related information infrastructure jointly.