• Korean Journal of Plant Resources
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• v.10 no.1
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• pp.39-44
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• 1997

This study was conducted to determine the effects of growth regulators, temperature, and light on the germination of Chwinamul(Aster scaber, Aster tataricus, Synurus deltoides, Solidago virga-aurea var. asiatica, Ligularia fisheri) seeds. Compared to GA, IAA, and kinetin, BA (150ppm) stimulated best seed germination of all five species under the both light and dark condition at $25^{\circ}C$. The effect of growth regulators was not observed significantly under the light condition except of Ligularia fisheri and Synurus deltoides while it was shown markedly under the dark condition. Germination rates of Ligularia fisheri and Synurus deltoides in different concentration of BA were relatively higher under the light at $15^{\circ}C$ while those of other species varied in different concentration and temperature.

• BMB Reports
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• v.46 no.10
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• pp.513-518
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• 2013

We investigated the protective effects of Gymnaster koraiensis against oxidative stress-induced hepatic cell damage. We used two different cytotoxicity models, i.e., the administration of tert-butyl hydroperoxide (t-BHP) and acetaminophen, in HepG2 cells to evaluate the protective effects of G. koraiensis. The ethyl acetate (EA) fraction of G. koraiensis and its major compound, 3,5-di-O-caffeoylquinic acid (DCQA), exerted protective effects in the t-BHP-induced liver cytotoxicity model. The EA fraction and DCQA ameliorated t-BHP-induced reductions in GSH levels and exhibited free radical scavenging activity. The EA fraction and DCQA also significantly reduced t-BHP-induced DNA damage in HepG2 cells. Furthermore, the hexane fraction of G. koraiensis and its major compound, gymnasterkoreayne B (GKB), exerted strong hepatoprotection in the acetaminophen-induced cytotoxicity model. CYP 3A4 enzyme activity was strongly inhibited by the extract, hexane fraction, and GKB. The hexane fraction and GKB ameliorated acetaminophen-induced reductions in GSH levels and protected against cell death.

• Korean journal of food and cookery science
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• v.30 no.4
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• pp.369-377
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• 2014

Obesity increases oxidative stress, which could contribute to the development of insulin resistance and hyperglycemia. The purpose of this study was to investigate the hypoglycemic and antioxidant effect of sanchae-namul (SN) in mice with diet-induced obesity. Five-week-old male C57BL/6J mice were fed a basal or high-fat and high-sucrose (HFHS) diet with or without 3% freeze-dried SN powder composed of chamnamul, daraesoon, miyeokchwi, bangpung namul, and samnamul for 12 weeks after a 1-week adaptation. After sacrifice, serum glucose and insulin were measured and the homeostasis model assessment for insulin resistance (HOMA-IR) was determined as well. Hepatic lipid peroxidation, glutathione (GSH), and activities of the antioxidant enzymes were determined. SN given at 3% of the total diet did not significantly influence body weight and food intake in mice fed the HFHS diet. Serum glucose and insulin levels, as well as HOMA-IR values, were significantly lower in the SN group than those in the HFHS group. Thiobarbituric acid reactive substances (TBARS) levels in the liver were decreased significantly in the SN group compared with those in the HFHS group. SN significantly increased the GSH levels and the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) in the liver compared with those in the HFHS group. Overall, these findings suggest that SN may be useful in alleviating insulin resistance and hyperglycemia in mice fed HFHS diet; further, the improvement of insulin resistance could partly occur by reducing the oxidative stress.

• The Korean Journal of Community Living Science
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• v.17 no.1
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• pp.101-108
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• 2006

This study was conducted to develop the regulating methods for the optimal harvest stage of new sprout for woody plants, and to clarify the effects of storage condition and period on the growth of new sprout formed of Aralia elata cv. 'Jeongkwang'. Japanese angelica tree were collected in the Jangheung district of Jeonnam on 20. March, 2005, and those cut branches were stored at $5{\pm}2^{\circ}C$ and 80 to 90% of the relative humidity, and were cut in water. Survival rate of cut branches for Aralia elata cv. 'Jeongkwang' was significantly increased when that was used for the cutting after the collection with no storage. And those survival rate was much more increased when that was stored at 80 to 90% of relative humidity than 40 to 50% of relative humidity. Number of new sprouts formed which were not stored immediately after the collection 3.2 to 3.4, and that were stored at cold storage room before cutting and cut on 1. June and 1. August were 5.1 to 5.3. Leaf growth of cut branches cut in water on 20. March started at ten days after cutting, and that on 1. June and 1. August at one to two days after cutting. Slow and mature sprout growth of Aralia elata cv. 'Jeongkwang' which were cut in water were different by the cold storage periods. Nineteen days were needed for the mature growth of sprout when cut in water on 20. March, and thirteen days on 1. August.

• Journal of Plant Biotechnology
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• v.43 no.3
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• pp.293-301
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• 2016

High-quality gene sets are necessary for functional research of genes. Although Perilla is a commonly cultivated oil crop and vegetable crop in Southeast Asia, the quality of its available gene set is insufficient. To construct a high-quality Perilla gene set, we sequenced mRNAs extracted from different tissues of Perilla citriodora, the wild species (2n = 20) of Perilla. To make a high-quality gene set for P. citriodora, we compared the quality of assemblies produced by Velvet and Trinity, the two well-known de novo assemblers, and improved the de novo assembly pipeline by optimizing k-mers and removing redundant sequences. We then selected representative transcripts for loci according to several criteria. The improved assembly yielded a total of 86,396 transcripts and 38,413 representative transcripts. We evaluated the assembled transcripts by comparing them to 638 homologous Arabidopsis genes involved in fatty acid and TAG biosynthesis pathways. High proportions of full-length genes and transcripts in the assembled transcripts matched known genes in other species, indicating that the P. citriodora gene set can be applied in future functional studies. Our study provides a reference P. citriodora gene set for further studies. It will serve as valuable genetic resource to elucidate the molecular basis of various metabolisms.

• Journal of Plant Biotechnology
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• v.43 no.3
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• pp.376-382
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• 2016

DNA methylation regulations gene expression, thus having pivotal roles in a myriad of physiological and pathological processes. In this study, the morphology and stress tolerance of transgenic rice overexpressing NtROS2a were determined. Transgenic plants exhibited less and shorter lateral shoots. Under various treatments, rice overexpressing NtROS2a showed alleviation of damage symptoms with higher survival rate. After drought and re-watering treatment, transgenic rice seedlings restored their normal growth. However, wild type plants could not be rescued. These findings indicate that overexpression of NtROS2a gene in rice seedlings can increase their tolerance to drought stresses.

• Natural Product Sciences
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• v.21 no.3
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• pp.155-161
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• 2015

Peroxynitrite (ONOO⁻)-scavenging activities of nine Compositae herbs consisting of three Ixeris, two Youngia, two Cirsium and one of each Lactuca and Taraxacum species were evaluated. The contents of their ONOO⁻ scavengers in the extracts were also determined on a HPLC using seven standard compounds, chlorogenic acid (CGA), chicoric acid (CA), luteolin 7-glucoside (luteolin-7-glc), luteolin 7-glucuronide (luteolin-7-glcU), luteolin, linarin and pectolinarin. Five of those compounds exhibited potent ONOO⁻-scavenging activities: IC₅₀, CA (0.76 μM), CGA (1.34 μM), luteolin (0.81 μM), luteolin-7-glc (0.86 μM) and luteolin-7-glcU (3.13 μM). Both CA and luteolin-7-glc were highly contained in I. dentata (19.71 mg/g and 13.58 mg/g, respectively), I. dentata var. albiflora (17.58 mg/g and 23.83 mg/g, respectively) and I. sonchifolia (65.71 mg/g and 6.99 mg/g, respectively). Among the nine herbs, those three Ixeris species had very low IC₅₀ values over the range of 0.48 - 1.74 μg/mL, suggesting that they could be potential therapeutic vegetables, particularly for preventing diabetic complications or obesity, which can be caused by an excess production of ONOO⁻.

• Plant Biotechnology Reports
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• v.4 no.1
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• pp.15-21
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• 2010

The over-expression of Arabidopsis CAX1 and CAX2 causes transgenic tomato plants to reveal severe $Ca^{2+}$ deficiency-like symptoms such as tip-burn and/or blossom end rot, despite there being sufficient $Ca^{2+}$ in each plant part. To correct the symptoms and to moderately enhance the calcium level, a worldwide vegetable tomato was genetically engineered using a modified Arabidopsis cation/$H^+$ antiporter sCAX2A, a mutant form of Arabidopsis CAX2. Compared with the wild-type, the sCAX2A-expressing tomato plants demonstrated elevated $Ca^{2+}$ levels in the fruits with almost no changes in the levels of $Mn^{2+}$, $Cu^{2+}$, and $Fe^{2+}$. Moreover, expression of sCAX2A in tomato plants did not show any significant alterations in their morphological phenotypes. Unlike 35S::sCAX1 construct, sCAX2A antiporter gene driven by 35S promoter can be a valuable tool for enriching $Ca^{2+}$ contents in the tomato fruit without additional accumulation of the undesirable cations.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.58-58
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• 2018

'다목이'는 일반 곰취를 모본으로 한대리곰취를 부본으로 교배하여 병에 강하고 수량성이 뛰어난 특성을 가진 곰취 신품종으로 고령지농업연구소에서 육성하였다. 경관농업은 청정 농산물의 생산, 가공과 서비스가 연계된 농업의 6차산업화를 대표하는 농업형태이다. 곰취를 이용한 산채류 경관농업을 구현하기 위해 '다목이'와 자생식물의 혼식을 통한 친환경 생산기술을 개발코자 하였다. 인공상토로 채운 화분에 곤달비와 삼백초, 백리향, 라벤더 등 국내 외 자생식물을 혼식하여 각 식물의 지상부와 지하부 생육을 조사하였다. 삼백초, 라벤더, 이질풀, 웜우드, 어성초는 '다목이'와 혼식하여 지상부 생체중 214.1~362.4g, 지하부 생체중 171.0~258.5g으로 생육이 대등하거나 양호한 결과를 보였다. 반면에 생강과 박하는 '다목이'의 생육량을 지상부 생체중 90.9~123.6g, 지하부 생체중 54.0~110.3g으로 현저히 감소시켰다. 백리향과 좀씀바귀, 제충국, 옥잠화는 '다목이'에 의해 생육이 극도로 저하되거나 고사하였다. 배초향은 '다목이'와 서로의 생육이 감소된 것으로 나타났다. 따라서 '다목이'와의 혼식은 경관농업의 목적에 따라 삼백초, 라벤더, 이질풀, 웜우드, 어성초가 적합한 것으로 생각되었다.

• Journal of People, Plants, and Environment
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• v.22 no.4
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• pp.341-354
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• 2019

Urban agriculture, which promotes communication in vulnerable classes and the formation of social networks has been gaining attention with an emphasis on healthy city, elderly-friendly city, safe city and happy city as future keywords about urban life. There is a growing interest in public awareness in many areas such as health, society, economy, and ecology. As an attempt to improve the diversity of urban gardens, this study begins with collecting suitable crops for urban gardens from "Imwongyeongjeji (林園經濟志)," an encyclopedia written by Yoo-Ku Seo, a scholar in the 18-19th century. Out of those recorded in "Gwanhyuji (灌畦志)," 128 kinds of crops with linkage of the historical achievements of the realists who gave their priority to public welfare were selected and 53 crops which had traditionality, historicality, health functionality and popularity were finally selected. The properties (cold, warm, clam) of the selected crops were evenly distributed, and there was no crop that was hot and cool. In addition, the number of crops that have a sweet taste was the highest, followed by spicy and bitter, but there was no salty vegetable, which can be attributed to the fact that 12 namuls (wild vegetables) that grow in seas were excluded in this study since they were not suitable for urban gardens. Urban gardens can be transformed from those that focus on primary production and secondary consumption activities into a new resource that offers educational and traditional values by applying humanities to urban agriculture as a content resource in the era of cultural consilience and convergence. It is expected to satisfy urban residents' intellectual and participatory needs and to enhance the diversity and utility of urban gardens by applying traditional knowledge to a new model of urban agriculture. We hope that further research will be conducted to develop new types and models of urban agriculture going forward.