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http://dx.doi.org/10.9724/kfcs.2014.30.4.369

Antioxidant Effects of Sanchae-namul in Mice Fed High-Fat and High-Sucrose Diet  

Choi, Ha-Neul (Department of Smart Food and Drugs, School of Food and Life Science, Inje University)
Kang, Su-Jung (Department of Smart Food and Drugs, School of Food and Life Science, Inje University)
Choe, Eunok (Department of Food and Nutrition, Inha University)
Chung, Lana (College of Hotel & Tourism Management, Kyung Hee University)
Kim, Jung-In (Department of Smart Food and Drugs, School of Food and Life Science, Inje University)
Publication Information
Korean journal of food and cookery science / v.30, no.4, 2014 , pp. 369-377 More about this Journal
Abstract
Obesity increases oxidative stress, which could contribute to the development of insulin resistance and hyperglycemia. The purpose of this study was to investigate the hypoglycemic and antioxidant effect of sanchae-namul (SN) in mice with diet-induced obesity. Five-week-old male C57BL/6J mice were fed a basal or high-fat and high-sucrose (HFHS) diet with or without 3% freeze-dried SN powder composed of chamnamul, daraesoon, miyeokchwi, bangpung namul, and samnamul for 12 weeks after a 1-week adaptation. After sacrifice, serum glucose and insulin were measured and the homeostasis model assessment for insulin resistance (HOMA-IR) was determined as well. Hepatic lipid peroxidation, glutathione (GSH), and activities of the antioxidant enzymes were determined. SN given at 3% of the total diet did not significantly influence body weight and food intake in mice fed the HFHS diet. Serum glucose and insulin levels, as well as HOMA-IR values, were significantly lower in the SN group than those in the HFHS group. Thiobarbituric acid reactive substances (TBARS) levels in the liver were decreased significantly in the SN group compared with those in the HFHS group. SN significantly increased the GSH levels and the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) in the liver compared with those in the HFHS group. Overall, these findings suggest that SN may be useful in alleviating insulin resistance and hyperglycemia in mice fed HFHS diet; further, the improvement of insulin resistance could partly occur by reducing the oxidative stress.
Keywords
sanchae (wild vegetable); thiobarbituric acid reactive substances (TBARS); superoxide dismutase (SOD); glucose; insulin;
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1 Zhang ZF, Lu J, Zheng YL, Wu DM, Hu B, Shan Q, Cheng W, Li MQ, Sun YY. 2013. Purple sweet potato color attenuates hepatic insulin resistance via blocking oxidative stress and endoplasmic reticulum stress in high-fat-diet-treated mice. J Nutr Biochem 24(6):1008-1018   DOI   ScienceOn
2 Shin JW, Lee SI, Woo MH, Kim SD. 2008. Effect of ethanol extracts of Goat's beard on streptozotocin induced diabetic symptoms and oxidative stress in rats. J East Asian Soc Dietary Life 18(6):939-948   과학기술학회마을
3 Standl E, Baumgartl HJ, Fuchtenbusch M, Stemplinger J. 1999. Effect of acarbose on additional insulin therapy in type 2 diabetic patients with late failure of sulphonylurea therapy. Diabetes Obes Metab 1(4):215-220   DOI   ScienceOn
4 Styskal J, Van Remmen H, Richardson A, Salmon AB. 2012. Oxidative stress and diabetes: what can we learn about insulin resistance from antioxidant mutant mouse models? Free Radic Biol Med 52(1):46-58   DOI   ScienceOn
5 Sun Y, Oberley LW, Li Y. 1988. A simple method for clinical assay of superoxide dismutase. Clin Chem 34(3):497-500
6 Surwit RS, Kuhn CM, Cochrane C, McCubbin JA, Feinglos MN. 1988. Diet-induced type II diabetes in C57BL/6J mice. Diabetes 37(9):1163-1167   DOI
7 Townsend DM, Tew KD, Tapiero H. 2003. The importance of glutathione in human disease. Biomed Pharmacother 57(3-4):145-155   DOI   ScienceOn
8 Yang JE, Lee JH, Kim DY, Choe EO, Chung LN. 2014. Sensory properties and drivers of liking Sanchae namul (seasoned dish with wild edible greens). Korean J Food Cook Sci 30(2):200-211   과학기술학회마을   DOI   ScienceOn
9 Yang ZH, Miyahara H, Takeo J, Katayama M. 2012. Diet high in fat and sucrose induces rapid onset of obesity-related metabolic syndrome partly through rapid response of genes involved in lipogenesis, insulin signalling and inflammation in mice. Diabetol Metab 4(1):32   DOI   ScienceOn
10 Maddux BA, See W, Lawrence JC Jr, Goldfine AL, Goldfine ID, Evans JL. 2001. Protection against oxidative stress-induced insulin resistance in rat L6 muscle cells by mircomolar concentrations of alpha-lipoic acid. Diabetes 50(2):404-410   DOI   ScienceOn
11 Manach C, Scalbert A, Morand C, Remesy C, Jimenez L. 2004. Polyphenols: food sources and bioavailability. Am J Clin Nutr 79(5):727-747
12 Moskaug JO, Carlsen H, Myhrstad MC, Blomhoff R. 2005. Polyphenols and glutathione synthesis regulation. Am J Clin Nutr 81(1 Suppl):277S-283S
13 Ohkawa H, Ohishi N, Yagi K. 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95(2):351-358   DOI   ScienceOn
14 Paglia DE, Valentine WN. 1967. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70(1):158-169
15 Park HJ, Kim HS. 2014. Korean traditional natural herbs and plants as immune enhancing, antidiabetic, chemopreventive, and antioxidative agents: a narrative review and perspective. J Med Food 17(1):21-27   DOI   ScienceOn
16 Raabo E, Terkildsen TC. 1960. On the enzymatic determination of blood glucose. Scand J Clin Lab Invest 12(4):402-407   DOI   ScienceOn
17 Rudich A, Tirosh A, Potashnik R, Hemi R, Kanety H, Bashan N. 1998. Prolonged oxidative stress impairs insulin-induced GLUT4 translocation in 3T3-L1 adipocytes. Diabetes 47(10):1562-1569   DOI   ScienceOn
18 Ruhe RC, McDonald RB. 2001. Use of antioxidant nutrients in the prevention and treatment of type 2 diabetes. J Am Coll Nutr 20(5 Suppl):363S-369S   DOI
19 Kahn SE, Hull RL, Utzschneider KM. 2006. Mechanisms linking obesity to insulin resistance and type 2 diabetes. Nature 444(7121):840-846   DOI   ScienceOn
20 Shah S, Iqbal M, Karam J, Salifu M, McFarlane SI. 2007. Oxidative stress, glucose metabolism, and the prevention of type 2 diabetes: pathophysiological insights. Antioxid Redox Signal 9(7):911-929   DOI   ScienceOn
21 Kim SJ, Min SC, Shin HJ, Lee YJ, Cho AR, Kim SY, Han J. 2013. Evaluation of the antioxidant activities and nutritional properties of ten edible plant extracts and their application to fresh ground beef. Meat Sci 93(3):715-722   DOI   ScienceOn
22 King H, Aubert RE, Herman WH. 1998. Global burden of diabetes, 1995-2025: prevalence, numerical estimates, and projections. Diabetes Care 21(9):1414-1431   DOI   ScienceOn
23 Kwak CS, Lee JH. 2014. In vitro antioxidant and antiinflammatory effects of ethanol extracts from sprout of Evening primrose (Oenothera laciniata) and Gooseberry (Actinidia arguta). J Korean Soc Food Sci Nutr 43(2):207-215   DOI   ScienceOn
24 Lebovitz HE. 1998. ${\alpha}$-Glucosidase inhibitors as agents in the treatment of diabetes. Diabetes Rev 6(2):132-145
25 Lee YM, Bae JH, Jung HY, Kim JH, Park DS. 2011. Antioxidant activity in water and methanol extracts from Korean edible wild plants. J Korean Soc Food Sci Nutr 40(1): 29-36   과학기술학회마을   DOI
26 Lu J, Qian W, Xu L, Huang G, Cong W, Wang Z, Deng X, Wang D, Guan S. 2012. Phytochemical composition and toxicity of an antioxidant extract from Pimpinella brachycarpa (Kom.) Nakai. Environ Toxicol Pharmacol 34(2):409-415   DOI   ScienceOn
27 Choi IY, Song YJ, Lee WH. 2010. DPPH radical scavenging effect and antimicrobial activities of some herbal extracts. Kor J Hort Sci Technol 28(5):871-876   과학기술학회마을
28 Bae DS. 2012. Isolation and identification of antioxidative compounds from Ledebouriella seseloides. Master's thesis. The Pusan National University of Korea. p 17, p 27, p 29
29 Chae HS, Lee SH, Jeong HS, Kim WJ. 2013. Antioxidant activity and physicochemical characteristics of Pimpinella brachycarpa Nakai with treatments methods. Korean J Food Nutr 26(1):125-131   과학기술학회마을   DOI   ScienceOn
30 Cheng D. 2005. Prevalence, predisposition and prevention of type II diabetes. Nutr Metab (Lond) 2(29):1-12   DOI   ScienceOn
31 Dembinska-Kiec A, Mykkanen O, Kiec-Wilk B, Mykkanen H. 2008. Antioxidant phytochemicals against type 2 diabetes. Br J Nutr 99 E Suppl (1):ES109-117
32 Droge W. 2002. Free radicals in the physiological control of cell function. Physiol Rev 82(1):47-95
33 Ellman GL. 1959. Tissue sulfhydryl groups. Arch Biochem Biophys 82(1):70-77   DOI   ScienceOn
34 Furukawa S, Fujita T, Shimabukuro M, Iwaki M, Yamada Y, Nakajima Y, Nakayama O, Makishima M, Matsuda M, Shimomura I. 2004. Increased oxidative stress in obesity and its impact on metabolic syndrome. J Clin Invest 114(12):1752-1761   DOI   ScienceOn
35 Haffner SM, Miettinen H, Stern MP. 1997. The homeostasis model in the San Antonio Heart Study. Diabetes Care 20(7):1087-1092   DOI   ScienceOn
36 Harman D. 1991. The aging process: major risk factor for disease and death. Proc Natl Acad Sci USA 88(12):5360-5363   DOI   ScienceOn
37 AOAC. 1995. Official methods of analysis. 14th ed. Association of official analytical chemists. Washington DC. USA
38 Hu ML. 2011. Dietary polyphenols as antioxidants and anticancer agents: more questions than answers. Chang Gung Med J 34(5):449-460
39 Aebi H. 1974. Catalase. Methods of enzymatic analysis. Vol 2. pp 673-684. In: Bergmeyer HU, Gawehn K, editors. Academic Press. New York, NY. USA
40 Ahn SY, Kim JH, Choi SJ, Kim YJ. 2009. Current status and prospect of cultivation of wild vegetable crops. Korean J Hortic Sci Technol 27(S):36-36
41 Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72(1-2):248-254   DOI   ScienceOn
42 Lee SJ, Choi HN, Kang MJ, Choe E, Auh JH, Kim JI. 2013. Chamnamul [Pimpinella brachycarpa (Kom.) Nakai] ameliorates hyperglycemia and improves antioxidant status in mice fed a high-fat, high-sucrose diet. Nutr Res Pract 7(6):446-452   과학기술학회마을   DOI   ScienceOn
43 Kim MS, Kim KH, Jo JE, Choi JJ, Kim YJ, Kim JH, Jang SA, Yook HS. 2011. Antioxidative and Antimicrobial Activities of Aruncus dioicus var. kamtschaticus Hara Extracts. J Korean Soc Food Sci Nutr 40(1):47-55   과학기술학회마을   DOI   ScienceOn
44 Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M. 2006. Free radicals, metals and antioxidants in oxidative stressinduced cancer. Chem Biol Interact 160(1):1-40   DOI   ScienceOn