Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Overexpression of NtROS2a gene encoding cytosine DNA demethylation enhances drought tolerance in transgenic rice

시토신 탈메틸화 관련 NtROS2a 유전자 도입 형질전환벼의 건조스트레스 내성 증진

  • Choi, Jang Sun (Vegetable Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Lee, In Hye (Department of Horticultural Life Science, Hankyong National University) ;
  • Cho, Yong-Gu (Department of Crop Science, Chungbuk National University) ;
  • Jung, Yu Jin (Department of Horticultural Life Science, Hankyong National University) ;
  • Kang, Kwon Kyoo (Department of Horticultural Life Science, Hankyong National University)
  • 최장선 (농촌진흥청 국립원예특작과학원 채소과) ;
  • 이인혜 (국립한경대학교 원예생명과학과) ;
  • 조용구 (충북대학교 식물자원학과) ;
  • 정유진 (국립한경대학교 원예생명과학과) ;
  • 강권규 (국립한경대학교 원예생명과학과)
  • Received : 2016.09.27
  • Accepted : 2016.09.29
  • Published : 2016.09.30
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Abstract

DNA methylation regulations gene expression, thus having pivotal roles in a myriad of physiological and pathological processes. In this study, the morphology and stress tolerance of transgenic rice overexpressing NtROS2a were determined. Transgenic plants exhibited less and shorter lateral shoots. Under various treatments, rice overexpressing NtROS2a showed alleviation of damage symptoms with higher survival rate. After drought and re-watering treatment, transgenic rice seedlings restored their normal growth. However, wild type plants could not be rescued. These findings indicate that overexpression of NtROS2a gene in rice seedlings can increase their tolerance to drought stresses.

DNA methylation은 무수히 많이 발생하는 생리적 및 병리적 측면의 과정에서 관련 유전자의 발현을 조절함으로써 중추적인 역할을 가지고 있다. 본 연구에서는 NtROS2a가 과발현된 형질전환 벼를 육성하고, 그들의 형태적 측면을 관찰하고 스트레스 내성을 검토하였다. 형질전환 식물체는 WT에 비하여 신초의 신장이 작게 나타났다. 저온 스트레스 처리 하에서 NtROS2a 형질전환 벼는 스트레스로 인한 생육 불량을 보였으나 건조 스트레스 처리 하에서는 WT보다는 높은 비율로 회복하여 생존하는 것을 확인할 수 있었다. 이러한 결과는 NtROS2a 유전자의 과발현으로 인해 벼가 건조 스트레스에 노출되면 내성을 증진시킨 것으로 생각된다.

Keywords

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