Browse > Article
http://dx.doi.org/10.1007/s11816-009-0112-9

Modest calcium increase in tomatoes expressing a variant of Arabidopsis cation/$H^+$ antiporter  

Chung, Mi-Young (Boyce Thompson Institute for Plant Research, Cornell University)
Han, Jeung-Sul (Department of Ecological Environment Conservation, Kyungpook National University)
Giovannoni, James (Boyce Thompson Institute for Plant Research, Cornell University)
Liu, Yang (Boyce Thompson Institute for Plant Research, Cornell University)
Kim, Chang-Kil (Department of Environmental Horticulture, Kyungpook National University)
Lim, Ki-Byung (School of Plant Biosciences, Kyungpook National University)
Chung, Jae-Dong (School of Plant Biosciences, Kyungpook National University)
Publication Information
Plant Biotechnology Reports / v.4, no.1, 2010 , pp. 15-21 More about this Journal
Abstract
The over-expression of Arabidopsis CAX1 and CAX2 causes transgenic tomato plants to reveal severe $Ca^{2+}$ deficiency-like symptoms such as tip-burn and/or blossom end rot, despite there being sufficient $Ca^{2+}$ in each plant part. To correct the symptoms and to moderately enhance the calcium level, a worldwide vegetable tomato was genetically engineered using a modified Arabidopsis cation/$H^+$ antiporter sCAX2A, a mutant form of Arabidopsis CAX2. Compared with the wild-type, the sCAX2A-expressing tomato plants demonstrated elevated $Ca^{2+}$ levels in the fruits with almost no changes in the levels of $Mn^{2+}$, $Cu^{2+}$, and $Fe^{2+}$. Moreover, expression of sCAX2A in tomato plants did not show any significant alterations in their morphological phenotypes. Unlike 35S::sCAX1 construct, sCAX2A antiporter gene driven by 35S promoter can be a valuable tool for enriching $Ca^{2+}$ contents in the tomato fruit without additional accumulation of the undesirable cations.
Keywords
Solanum lycopersicum; Genetic transformation; Cation/$H^+$ antiporter; $Ca^{2+}$ nutrition; Blossom-end rot; Tip-burn;
Citations & Related Records

Times Cited By Web Of Science : 3  (Related Records In Web of Science)
연도 인용수 순위
  • Reference
1 Shigaki T, Pittman JK, Hirschi KD (2003) Manganese specificity determinants in the Arabidopsis metal/$H^{+}$ antiporter CAX2. J Biol Chem 278:6610-6661   DOI   ScienceOn
2 Pittman JK, Shigaki T, Marshall JL, Morris JL (2004) Functional and regulatory analysis of the Arabidopsis thaliana CAX2 cation transporter. Plant Mol Biol 56:959-971   DOI   ScienceOn
3 Schaaf G, Catoni E, Fitz M, Schwacke R, Schneider A, von Wire N, Frommer WB (2002) A putative role for the vacuolar calcium/ manganese proton antiporter AtCAX2 in heavy metal detoxification. Plant Biol 4:612-618   DOI   ScienceOn
4 Shear CB (1975) Calcium-related disorders of fruits and vegetables. HortScience 10:361-365
5 Lee KD, Lee YB, Yang MS, Kim PJ (2002) Effect of soil amendment application on yields and effective components of Chrysanthemum boreale M. Korean J Soil Sci Fertil 35:27-37
6 Marschner H (1995) Mineral nutrition of higher plants. Academic, New York
7 Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473-497   DOI
8 Nonami H, Fukuyama T, Yamamoto M, Yang L, Hashimoto Y (1995) Blossom-end rot of tomato plants may not be directly caused by calcium deficiency. Acta Hortic 395:107-114
9 Park S, Kim C-K, Pike LM, Smith RH, Hirschi KD (2004) Increased calcium in carrots by expression of an Arabidopsis $H^{+}/Ca^{2+}$ transporter. Mol Breed 14:275-282   DOI
10 Hirschi KD (2004) The calcium conundrum: both versatile nutrient and specific signal. Plant Physiol 136:2438-2442   DOI   ScienceOn
11 Kim CK, Han JS, Lee HS, Oh JY, Shigaki T, Park SH, Hirschi KD (2006) Expression of an Arabidopsis CAX2 variant in potato tubers increases calcium levels with no accumulation of manganese. Plant Cell Rep 25:1226-1232   DOI   ScienceOn
12 Hirschi KD, Korenkov VD, Wilganowski NL, Wagner GJ (2000) Expression of Arabidopsis CAX2 in tobacco. Altered metal accumulation and increased manganese tolerance. Plant Physiol 124:125-133   DOI
13 Ho LC, White PJ (2005) A cellular hypothesis for the induction of blossom end rot in tomato fruit. Ann Bot (Lond) 95:571-581   DOI   ScienceOn
14 Holsters M, De Waele D, Depickev A, Messens E, Van Montagu M, Schell J (1978) Transfection and transformation of A. tumefaciens. Mol Genet Genomics 163:181-187   DOI   ScienceOn
15 Fleming KH, Heimback JT (1994) Consumption of calcium in the US: food sources and intake levels. J Nutr 124:1426S-1430S   DOI
16 Adams P, Ho LC (1993) Effects of environment on the uptake and distribution of calcium in tomato and on the incidence of blossom-end rot plant and soil. Plant Soil 154:127-132   DOI   ScienceOn
17 Cheng NH, Pittman JK, Shigaki T, Lachmansingh J, LeClere S, Lahner B, Salt DE, Hirschi KD (2005) Functional association of Arabidopsis CAX1 and CAX3 is required for normal growth and ion homeostasis. Plant Physiol 138:2048-2060   DOI   ScienceOn
18 Feinberg AP, Vogelstein B (1983) A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6-13   DOI   ScienceOn
19 Flowers TJ, Colmer TD (2008) Salinity tolerance in halophytes. New Phytol 179:945-963   DOI   ScienceOn
20 Fulton T, Julapark C, Tanksley S (1995) Miniprep protocol for extraction of DNA from tomato and other herbaceous plants. Plant Mol Biol Rep 13:207-209   DOI   ScienceOn
21 Hirschi KD (1999) Expression of Arabidopsis CAX1 in tobacco: altered calcium homeostasis and increased stress sensitivity. Plant Cell 11:2113-2122   DOI
22 Park S, Cheng NH, Pittman JK, Yoo KS, Park J, Smith RH, Hirschi KD (2005) Increased calcium levels and prolonged shelf life in tomatoes expressing Arabidopsis $H^{+}/Ca^{2+}$ transporters. Plant Physiol 139:1194-1206   DOI   ScienceOn
23 Yamaguchi T, Fukada-Tanaka S, Inagaki Y, Saito N, Yonekura- Sakakibara K, Tanaka Y, Kusumi T, Iida S (2001) Genes encoding the vacuolar $Na^{+}/H^{+}$ exchanger and flower coloration. Plant Physiol 42:451-461
24 Weaver CM, Proulx WR, Heaney R (1999) Choices for achieving adequate dietary calcium with a vegetarian diet. Am J Clin Nutr 70:543S-548S   DOI
25 Wyatt SE, Tsou P-L, Robertson D (2002) Expression of the highcapacity calcium-binding domain of calreticulin increases bioavailable calcium stores in plants. Transgenic Res 11:1-10   DOI   ScienceOn
26 Simon EW (1978) The symptoms of calcium deficiency in plants. New Phytol 80:1-15   DOI   ScienceOn
27 Wang H, Inukai Y, Yamauchi A (2006) Root development and nutrient uptake. Crit Rev Plant Sci 25:279-301   DOI   ScienceOn
28 Hirschi KD, Zhen R-G, Cunningham KW, Rea PA, Fink GR (1996) CAX1, an $H^{+}/Ca^{2+}$ antiporter from Arabidopsis. Proc Natl Acad Sci USA 93:8782-8786   DOI   ScienceOn