• 대한한의학방제학회지
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• 제23권1호
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• pp.101-110
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• 2015

Objectives : The purpose of this study was to investigate the anti-cancer effects of Oldenlandia diffusa extract on WiDr human colorectal adenocarcinoma cells. Methods : We examined cell death by (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide) MTT assay and the caspase 3 and 9 activity assay with Oldenlandia diffusa extract. To examine the inhibitory effects of Oldenlandia diffusa extract, we performed a cell cycle (sub-G1) analysis and mitochondrial membrane potential for the WiDr cells after 24 hours with Oldenlandia diffusa extract. Results : 1. Oldenlandia diffusa extract induced cell death in WiDr cells. 2. The sub-G1 peak was increased by Oldenlandia diffusa extract in WiDr cells. 3. Oldenlandia diffusa extract leads to increase the mitochondrial membrane depolarization in WiDr cells. 4. Oldenlandia diffusa extract increases caspase 3 and 9 activities in WiDr cells. 5. Oldenlandia diffusa extract combined with several anti-cancer drugs (paclitaxel, 5-fluorouracil, cisplatin, ectoposide, doxorubicin and docetaxel) markedly inhibited the growth of WiDr cells compared to Oldenlandia diffusa extract and anti-cancer drugs alone. Conclusions : Oldenlandia diffusa extract has an apoptotic role in human colorectal cancer cells and a potential role in developing therapeutic agents against colorectal cancer.

• 한국축산식품학회지
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• 제22권1호
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• pp.72-76
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• 2002

상업적으로 생산 판매되고 있는 여러 가지 유청 단백질을 이용하여 각종 암세포에 대한 세포독성을 조사한 결과, IgG, BSA, $\beta$-LG는 세포독성이 거의 없었으나, $\alpha$-LA가 강한 세포독성을 갖고 있음이 확인되었다. MKN45, HeLa, WiDr, 그리고 A498의 경우, $\alpha$-LA(1mg/ml)에 의해 세포성장이 95% 이상 억제되는 것으로 나타났다. HPLC를 이용하여 $\alpha$-LA에 혼입되어 있는 미량성분을 제거한 후, 세포독성을 조사한 결과, $\alpha$-LA이 나타내었던 세포독성이 대부분 없어지는 것으로 나타났다. 또한 $\alpha$-LA을 trypsin으로 부분 가수분해한 후, 각종 암세포에 대한 성장 억제효과를 검토한 결과, 암세포에 대한 독성을 상실하는 것으로 나타났으며, $\alpha$-LA를 EDTA로 처리할 경우, $\alpha$-LA의 세포독성이 대부분 상실되는 것으로 나타났다.

• Radiation Oncology Journal
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• 제26권3호
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• pp.166-172
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• 2008

목 적: 암환자의 방사선 치료 전 방사선에 대한 감수성을 미리 측정할 수 있다면 임상적으로 많은 도움이 될 것이다. 본 연구는 전 임상 실험을 통하여 MTT assay가 세포집락 측정기법과 비교해서 방사선 감수성을 예측할 수 있고, 직장암 환자의 조직에 사용할 수 있는지 가능성을 확인하고자 하였다. 대상 및 방법: 대장암 세포 주인 HCT-8, LoVo, CT-26, WiDr을 이용하여 세포집락 측정기법을 통해 세포생존곡선 및 2 Gy에서의 세포생존확률(SF2)을 구하였다. 세포 주 자체를 대상으로 MTT assay를 시행하는 실험(in vitro) 및 환자의 암 조직과 같은 상태를 만들기 위하여, 누드 마우스에 세포 주를 주입하여 암 조직을 형성한 후 in vitro와 같은 방식으로 MTT assay를 시행(ex vivo)하였다. 이 두 실험에 대한 흡광도 값에 따른 저해율(inhibition rate, %)을 구하였다. 결 과: $SF_2$ 및 세포생존곡선에 따르면 CT-26 및 LoVo가 HCT-8, WiDr에 비해 방사선에 민감하였다(p<0.05). In vitro MTT assay 결과 WiDr, HCT-8, LoVo와 CT-26의 방사선 저해율이 각각 17.3%, 21%, 30%, 56.5%를 나타내었다. 또한 ex vivo MTT assay의 저해율은 HCT-8, WiDr, LoVo와 CT-26에서 각각 23.5%, 26%, 38%, 53%를 나타내었다. 통계적인 차이를 감안하였을 때 세포생존곡선을 통해 얻은 방사선 감수성의 결과와 동일한 순서를 가졌다. 결 론: 4개의 세포 주의 방사선의 감수성의 순서가 세포집락 측정기법 및 in vitro와 ex vivo MTT assay 결과에서 거의 일치함을 보였다. 이는 직장암 환자에서 MTT assay를 통해 방사선 감수성을 예측할 수 있는 가능성을 제시하였다.

• 한국한의학연구원논문집
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• 제17권2호
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• pp.129-134
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• 2011

Objective : The purpose of this study was to investigate the anti-cancer effects of Carthami Flos in some kinds of human colorectal adenocarcinoma cells. Method : We used two kinds of human colorectal adenocarcinoma cell lines, such as HT-29 and WiDr cells. We examined cell death by MTT assay and observed the morphological changes with Carthami Flos. Result : We showed that the combination of sub-optimal doses of Carthami Flos and cisplatin noticeably suppresses in HT-29 cells and doxorubicin in WiDr cells. Furthermore, we studied the caspase 3 activity to identify the apoptosis. Conclusion : Our findings provide insight into unraveling the effects of Carthami Flos in human colorectal adenocarcinoma cells and developing therapeutic agents against colorectal cancer.

• Transactions on Electrical and Electronic Materials
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• 제18권6호
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• pp.359-363
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• 2017

A slotted implantable patch antenna with microstrip feeding is proposed for industrial, scientific, and medical band applications. The result is verified by implanting the antenna in animal tissue. Further, by varying the ground width and introducing a defect into the ground structure, the antenna becomes applicable for worldwide interoperability for microwave access operations. A simulation is performed using Empire XCcel software. An Agilent vector network analyzer is used for analyzing the return loss performance. Simulated and measured results are compared. Antennas with and without defected ground structure both have key advantages including low profile, desirable return loss, good impedance matching and required bandwidth.

• 전기학회논문지
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• 제58권12호
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• pp.2354-2358
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• 2009

Demand response (DR) can be used to improve the efficiency of electricity markets and increase the reliability of power systems. As more utilities attempt to reduce the purchasing costs by implementing DR programs strategically, there is an increasing need for studies of how to allocate the reduced purchasing costs among DR program participants. The rebates or incentives can be given to DR program participants in proportion to the participants' contributions to the reduced purchasing costs. This paper presents Shapley Value-based method to determine the DR program participants' contributions to the reduced purchasing costs. A numerical example is presented to validate the effectiveness of the proposed method.

• Journal of Ginseng Research
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• 제22권3호
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• pp.188-192
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• 1998

We established the model of clonogenic assay with human tumor cell line such as Calu-3 (lung carcinoma), HEC- lB (endometrial adenocarcinoma) , HEp-2 (larnyx carcinoma), Hs-5787 (breast carcinoma), K-562 (chronic myelogenous leukemia), SF-188 (brain carcinoma), SNU-1 (stomach carcinoma) and WiDr (colon carcinoma) . We investigated growth inhibition of solvent (EtOH, MeOH) and water (100$^{\circ}C$, 121$^{\circ}C$) extracts from Korean red ginseng by clonogenic assay. The results of clonogenic assay showed that EtOH extract had growth inhibition against Calu-3, SF-188 and SNU-1, MeOH extract had growth inhibition against Calu-3, Hs-5787, K-562, and WiDr, but water extract at 100$^{\circ}C$ and water extract at 121$^{\circ}C$ had not growth inhibition against used cell lines.

• Journal of Ginseng Research
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• 제19권1호
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• pp.27-30
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• 1995

We established the model of clonogenic assay with cancer cell lines such as SW-156(kidney), SNU-5(stomach), Hep G2(liver), and WiDr(colon), and we investigated anticancer effect of hydrophobic protein fraction(N-fraction) from Korea red ginseng by using this model. The results of clonogenic assay showed that N-fraction had anticancer activity against SNU-5 above 100 $0.2\mu\textrm{g}$/ml concentration, and did not exhibit anticancer activity against cell lines such as SW-156, WiDr, and Hep G2 up to 1,000 $\mu\textrm{g}$/ml concentration. This result suggests that N-fraction has specially anti-stomach cancerous effect.

• 제어로봇시스템학회논문지
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• 제22권11호
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• pp.967-976
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• 2016

This paper presents a 3D carrier-smoothed GNSS/DR (Global Navigation Satellite System/Dead Reckoning) integrated system for precise ground-vehicle trajectory estimation. For precise DR navigation on sloping roads, the AHRS (Attitude Heading Reference System) methodology is employed. By combining the integrated carrier phase of GNSS and DR sensor measurements, a vehicle trajectory with an accuracy of less than 20cm is obtained even when cycle slip or change of visibility occur. In order to supplement the weak GNSS environment with DR successfully, the DR sensor is precisely compensated for using GNSS Doppler measurements when GNSS visibility is good. By integrating a multi-GNSS receiver with low-cost IMU, a precise 3D navigation system for land vehicles is proposed in this paper. For real-time implementation, a decoupled Kalman filter is employed in the integrated system. Through field experiments, the performance of the proposed system is verified in various road environments, including sloping roads, good-visibility areas, high multi-path areas, and under-ground parking areas.

• 생명과학회지
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• 제15권6호
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• pp.857-862
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• 2005

본 연구는 whole molecular-, 40 kDa-, 10 kDa poly-mannuronate를 대장암세포에 첨가하였을 때 분자량과 농도 에 따른 항암효과를 세포수준에서 검토하고자 하였다. 3종류의 인체 대장암세포인 HT-29, DLD-1, WiDr의 증식 억제를 살펴본 결과, HT-29 세포는 모든 polymannuronate $0.25\%$의 농도에서 $50\%$의 증식 억제 효과를 보였으며 저분자화 될 수 록 증식 억제 효과가 증가되었다. DLD-1 세포는 저분자화 정도와 농도증가에 따른 유의적인 차이를 보이지 않았고, WiDr 세포는 농도증가에 따른 유의적인 효과는 있었으나, 분자량에 의한 차이는 보이지 않았다. $0.25\%$의 농도에서 $50\%$의 증식 억제 효과를 보인 HT-29 세포의 DNA 합성능을 살펴본 결과, 증식 억제 효과와 유사한 결과를 보였으며, 형태학적인 변화 또한 동일한 것으로 나타났다. 이상의 결과로부터 polymannuronate는 HT-29 대장암세포의 증식억제에 유의적인 효과가 있는 것으로 나타났다. 따라서 polymannuronate는 대장암의 예방뿐 아니라 그 치료에도 도움이 되며 본 연구가 polymannuronate의 효과에 대한 기초적인 자료가 될 것으로 사료된다.