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Effects of Carthami Flos on Human Colorectal Adenocarcinoma Cells  

Han, Song-Ee (Division of Longevity and Biofunctional Medicine School of Korean Medicine, Pusan National University)
Kim, Jung-A (Division of Longevity and Biofunctional Medicine School of Korean Medicine, Pusan National University)
Song, Ho-Joon (Division of Longevity and Biofunctional Medicine School of Korean Medicine, Pusan National University)
Chae, Han (Division of Longevity and Biofunctional Medicine School of Korean Medicine, Pusan National University)
Kwon, Young-Kyu (Division of Longevity and Biofunctional Medicine School of Korean Medicine, Pusan National University)
Kim, Byung-Joo (Division of Longevity and Biofunctional Medicine School of Korean Medicine, Pusan National University)
Publication Information
Korean Journal of Oriental Medicine / v.17, no.2, 2011 , pp. 129-134 More about this Journal
Abstract
Objective : The purpose of this study was to investigate the anti-cancer effects of Carthami Flos in some kinds of human colorectal adenocarcinoma cells. Method : We used two kinds of human colorectal adenocarcinoma cell lines, such as HT-29 and WiDr cells. We examined cell death by MTT assay and observed the morphological changes with Carthami Flos. Result : We showed that the combination of sub-optimal doses of Carthami Flos and cisplatin noticeably suppresses in HT-29 cells and doxorubicin in WiDr cells. Furthermore, we studied the caspase 3 activity to identify the apoptosis. Conclusion : Our findings provide insight into unraveling the effects of Carthami Flos in human colorectal adenocarcinoma cells and developing therapeutic agents against colorectal cancer.
Keywords
Carthami Flos; Human Colorectal Adenocarcinoma Cell; HT-29; WiDr;
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Times Cited By KSCI : 3  (Citation Analysis)
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1 Takii T, Hayashi M, Hiroma H, Chiba T, Kawashima S, Zhang HL, Nagatsu A, Sakakibara J, Onozaki K. Serotonin derivative, N-(p-Coumaroyl)serotonin, isolated from safflower (Carthamus tinctorius L.) oil cake augments the proliferation of normal human and mouse fibroblasts in synergy with basic fibroblast growth factor (bFGF) or epidermal growth factor (EGF). J Biochem. 1999:125(5): 910-5.   DOI
2 Kawashima S, Hayashi M, Takii T, Kimura H, Zhang HL, Nagatsu A, Sakakibara J, Murata K, Oomoto Y, Onozaki K. Serotonin derivative, N-(p-coumaroyl) serotonin, inhibits the production of TNF-alpha, IL-1alpha, IL-1beta, and IL-6 by endotoxin-stimulated human blood monocytes. J Interferon Cytokine Res. 1998:18 (6):423-8.   DOI   ScienceOn
3 Jones JM, Anderson JW, Tukey RH. Using the metabolism of PAHs in a human cell line to characterize environmental samples. Environ Toxicol Pharmacol. 2000:8(2):119-126.   DOI   ScienceOn
4 정승용. 대장암 조기검진을 위한 권고안. 대한소화기내시경학회지. 2002:24:317-320.
5 김정범, 안규석. 대장암의 동서의학적비교연구. 동의병리학회지. 1995:10:89-126.
6 박상구, 이지현, 문구, 문석재, 원진희, 박래길. 백화사설초 메탄올 추출물에 의한 HL-60세포고사과정에 있어서의 transcriptional factors 활성변화 연구. 대한한방종양학회지. 2000:6(1):67-80.
7 LeBrun DP, Warnke RA, Cleary ML. Expression of bcl-2 in fetal tissues suggests a role in morphogenesis. Am J Pathol. 1993:142(3):743-753.
8 김병주, 문구. 대장암의 동서의 결합진료 현황. 대한한방종양학회지. 1995:5:1-17.
9 Heo J. Dong-I Bogam 5. 3th eds., In: Safflower. Yekang Publishers, Seoul, Korea. 2763-2764, 1986.
10 Kee CH. The Pharmacology of Chinese Herbs. CRC press, 1993:249-250.
11 Kim MN, Kim KH. Researches for analgesic and hepatoprotective action of Carthami Flos. Pusan Bull. Pharm. Sci. 1992:26:32-36.
12 Cleary MP, Phillips FC, Morton RA. Genotype and diet effects in lean and obese Zucker rats fed either safflower or coconut oil diets. Proc. Soc. Exp. Biol. Mec. 1999:220:153-161.   DOI   ScienceOn
13 한종현, 유광석, 강성용. 홍화가 인체의 암세포주에 미치는 영향. 대한한의학회지. 1996:17(2):303-310.
14 보건복지부, 중앙암등록본부, 국립암센터. 국가암등록 사업 연계 보고서(2008년 국가암등록통계). 국민건강보험공단. . 2010.12
15 대한대장항문학회. 암진단양상조사결과. 대한대장항문학회. . 2010.9.1.
16 정명채, 김진성, 윤상협, 류봉화, 류기원. 반모가 수종의 인체 암세포에 미치는 영향. 대한한방내과학회지 2004:25(2):202-213.
17 Lee TB. Illustrated flora of Korea. Seoul: Hyangnounsa. 1989.
18 Kim JB, Cho MH, Hahn TR, Paik YS. Efficient purification and chemical structure identification of carthamin from carthamus tinctorius. Agric. Chem. Biotechnol. 1996:39:501-505.
19 Namba T. Coloured illustrations of wakan-yaku 1st ed. Vol.2. Osaka:Hoikusha Publishing Co., Ltd. 1986
20 Zhang HL, Nagatsu A, Watanabe T, Sakakibara J, Okuyama H. Antioxidative compounds isolated from safflower (Carthamus tinctorius L.) oil cake. Chem Pharm Bull (Tokyo). 1997:45(12):1910-4.   DOI   ScienceOn
21 Kasahara Y, Kumaki K, Katagiri S, Yasukawa K, Yamanouchi S, Takido M, Akihisa T, Tamura T. Carthami flos extract and its component, stigmasterol, inhibit tumour promotion in mouse skin two-stage carcinogenesis. Phytotherapy Research 1994:8:327-331.   DOI   ScienceOn
22 Cox C, Mann J, Sutherland W, Chisholm A, Skeaff M. Effects of coconut oil, butter, and safflower oil on lipids and lipoproteins in persons with moderately elevated cholesterol levels. J. Lipid Res. 1995:36:1787-1795.
23 Iwata T, Ohya K, Takehisa F, Tsutsumi K, Furukawa Y, Kimura S. The effect of dietary safflower phospholipid on sterols in gastrointestinal tract of rats fed a hypercholesterolemic diet. J. Nutr. Sci. Vitaminol. 1992:38:615-622.   DOI   ScienceOn
24 최영주, 최상욱. 홍화(Carthamus tinctorius L.)씨의 sterol 및 phytoestrogen 분석. Korean Journal of Life Science 2003:13(4):529-534.   DOI