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Antitumor Activity of $\alpha$-Lactalbumin on the Tumor Cells  

이수원 (성균관대학교 생명공학부)
신영하 (성균관대학교 생명공학부)
황보식 (성균관대학교 생명공학부)
Publication Information
Food Science of Animal Resources / v.22, no.1, 2002 , pp. 72-76 More about this Journal
Abstract
Bovine serum albumin(BSA), $\alpha$-lactalbumin($\alpha$-LA), $\beta$-lactoglobulin($\beta$-LG) and bovine immunoglobulin G (IgG) were investigated the cytotoxicity on tumor cell lines. $\alpha$-LA was showned a tendency of dose dependaent on cytotoxicity using WiDr. The growth of WiDr was inhibited 82% by 1mg/ml of $\alpha$-LA. However, IgG, BSA and $\beta$-LG were not shown the cytotoxicity on WiDr. When $\alpha$-LA was purified by using high pressure liquid chromatography(HPLC), the main component($\alpha$-LA) was eluted at 33.057 min and extremely small quantities eluted at 32.310 min. The cytotoxicity of main component (eluted at 33.057 min peak) was lower than commercial $\alpha$-LA. And the cytotoxic activity of hydrolyzed $\alpha$-LA and $\alpha$-LA treated with EDTA were lower than commercial $\alpha$-LA on tumor cells.
Keywords
${\alpha}$-lactalbumin; cytotoxicity; whey protein; MTT assay;
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