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http://dx.doi.org/10.14374/HFS.2015.23.1.101

Anti-Cancer Effects of Oldenlandia diffusa extract on WiDr human colorectal adenocarcinoma cells  

Lee, Soojin (Division of Longevity and Biofunctional Medicine, School of Korean Medicine, Pusan National University)
Gim, Huijin (Division of Longevity and Biofunctional Medicine, School of Korean Medicine, Pusan National University)
Shim, Ji Hwan (Division of Longevity and Biofunctional Medicine, School of Korean Medicine, Pusan National University)
Park, Hyun Soo (Division of Longevity and Biofunctional Medicine, School of Korean Medicine, Pusan National University)
Kim, Byung Joo (Division of Longevity and Biofunctional Medicine, School of Korean Medicine, Pusan National University)
Publication Information
Herbal Formula Science / v.23, no.1, 2015 , pp. 101-110 More about this Journal
Abstract
Objectives : The purpose of this study was to investigate the anti-cancer effects of Oldenlandia diffusa extract on WiDr human colorectal adenocarcinoma cells. Methods : We examined cell death by (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide) MTT assay and the caspase 3 and 9 activity assay with Oldenlandia diffusa extract. To examine the inhibitory effects of Oldenlandia diffusa extract, we performed a cell cycle (sub-G1) analysis and mitochondrial membrane potential for the WiDr cells after 24 hours with Oldenlandia diffusa extract. Results : 1. Oldenlandia diffusa extract induced cell death in WiDr cells. 2. The sub-G1 peak was increased by Oldenlandia diffusa extract in WiDr cells. 3. Oldenlandia diffusa extract leads to increase the mitochondrial membrane depolarization in WiDr cells. 4. Oldenlandia diffusa extract increases caspase 3 and 9 activities in WiDr cells. 5. Oldenlandia diffusa extract combined with several anti-cancer drugs (paclitaxel, 5-fluorouracil, cisplatin, ectoposide, doxorubicin and docetaxel) markedly inhibited the growth of WiDr cells compared to Oldenlandia diffusa extract and anti-cancer drugs alone. Conclusions : Oldenlandia diffusa extract has an apoptotic role in human colorectal cancer cells and a potential role in developing therapeutic agents against colorectal cancer.
Keywords
Oldenlandia diffusa; Human colorectal adenocarcinoma cancer cell; WiDr; Anti-cancer drugs;
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Times Cited By KSCI : 5  (Citation Analysis)
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