• Title/Summary/Keyword: Wi-gi

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Effect of EDTA on canine parthenote development during in vitro culture (개 parthenote in vitro culture시 EDTA 첨가에 의한 발달율 향상)

  • Jeong, Haeyun;Zhao, Minghui;No, Jin-Gu;Ullah, Imran;Lee, Whi-Cheul;Wi, Hayeon;Ock, Sun A;Hur, Tai-young;Woo, Jae-Seok;Im, Gi-sun;Kim, Jong-Gug;Lee, Seunghoon
    • Journal of Embryo Transfer
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    • v.33 no.3
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    • pp.139-147
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    • 2018
  • Somatic cell nuclear transfer (SCNT) is a useful biotechnological tool for animal cloning. Until now, SCNT has been inefficient, especially in dog. It is believed that an embryo developmental block in SCNT embryos is cause of low production efficiency. However, no studies have been performed on canines for embryo developmental block. In this study, we attempted to evaluate the beneficial role of EDTA in canine parthenogenic (PA) embryos development to overcome embryo developmental block. The PA embryos were divided into 0.01 mM EDTA treated and non-treated groups. Embryo developmental efficiency was measured by activating chemically parthenote. After EDTA induction, PA embryos were evaluated for embryonic development, Reactive Oxygen Species (ROS) activity, mitochondrial integrity, ATP production and genomic activation. The EDTA treated PA embryos showed significantly higher survival rate and improved cavity formation compared to non-treated. Furthermore, cytoplasmic ROS level was mitigated and mitochondrial membrane potential was found significantly higher in EDTA treated group followed by higher ATP production. Moreover, major embryonic genomic activation specific markers/factors were also elevated in EDTA treated group. Conclusively, we elucidated that EDTA showed substantially positive effect to overcome embryo developmental block in canine.

Study on the Chemically Method of Epoxy Restoration Material in Antic Ceramics and Stabilization of Their Materials (토기.도자기 복원에 사용된 에폭시 복원재료의 화학적 제거방법과 그 안정성에 관한 연구)

  • Han, Won-Sik;Bae, Jin-Soo;Park, Gi-Jung;Hong, Tae-Kee;Wi, Koang-Chul
    • Journal of Conservation Science
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    • v.26 no.1
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    • pp.25-32
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    • 2010
  • The yellowing is always occurred phenomena in epoxy restoration material of antic-ceramics. For re-restoration of remains with epoxy restoration material, the epoxy elimination step is the first one in all course of restoration In this paper, the chemically elimination treatments methods of epoxy in various antic-ceramics were discussed. We fabricated the epoxy elimination material for antic-ceramics restoration which were made up Dichloromethane and Dichlormethane based Dimethylformamide solution. Dichloromethane and Dichloromethane + Dimethylformamide mixture were applied to epoxy in various antic-ceramic sample. Particularly, Dichloromethane + Dimethylformamide solution had the best result in variation of color change, gloss, vending strength, weight, exfoliation time test after deposition in this solution. Dichloromrthane had the volume increase characteristics for epoxy material and Dimethylformamide had the mollification ones. So, Dichloromathane increased exfoliation in approximal surface of the epoxy material and antic ceramic, and Dimethylformamide decreased the surface hardness of epoxy. In this result, epoxy material even inside of ceramic that have very weak inside bonding is adaptable and stable eliminated. And in order to show the perfect elimination of this material, we successfully dissolve the epoxy restoration material in one antic pottery that is in one university museum's possession using this mixture. So, there is guarantee in the eternity and stabilization of restoration for antic-ceramics.

The Development of Water Quality Monitoring System and its Application Using Satellite Image Data

  • Jang, Dong-Ho;Jo, Gi-Ho
    • Proceedings of the KSRS Conference
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    • 1998.09a
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    • pp.376-381
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    • 1998
  • In this study, we was measured the radiance reflectance by using multi-spectral image of low resolution camera(LRC) which will be loaded in the multi-purpose satellite(KOMPSAT) to use the data in analyzing water pollution. Also we investigated the possibility of extraction of water quality factors in rivers and water body by using high resolution remote sensing data such as Airborne MSS. Especially, we tried to extract the environmental factors related with eutrophication, and also tried to develop the process technique and the radiance feature of reflectance related with eutrophication. The results were summarized as follows: First, the spectrum of sun's rays which reaches the surface of the earth was consistent with visible rays bands of 0.4${\mu}{\textrm}{m}$~0.7${\mu}{\textrm}{m}$ and about 50% of total quantity of radiation were there. And at around 0.5${\mu}{\textrm}{m}$ of green spectral band in visible rays bands, the spectrum was highest. Second, as a result of the radiance reflectance Chlorophyll-a represented high spectral reflectance mainly around 0.52${\mu}{\textrm}{m}$ of green spectral band, and suspended sediments and turbidity represented high spectral reflectance at 0.8${\mu}{\textrm}{m}$ and at 0.57${\mu}{\textrm}{m}$ each. Third, as a result of the water quality analysis by using Airborne MSS, Chlorophyll-a could have a distribution chart when carried out ratio of B3 and BS to B7. And Band 7 was useful for making the distribution chart of suspended sediments. And when we carried out PCA, suspended sediments and turbidity had distributions at PC 1 , PC 4 each similarly to ground truth data. Above results can be changed according to the change of season and time. Therefore, in order to analyze more exactly the environmental factors of water quality by using LRC data, we need to investigate constantly the ground truth data and the radiance feature of reflectance of water body. Afterward in this study, we will constantly analyze the radiance feature of the surface of water in water body by measuring the on-the-spot radiance reflectance and using low resolution satellite image(SeaWiFs). Besides, we will gather the data of water quality analysis in water body and analyze the pattern of water pollution.

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Study on the Urethane Restoration Filling Material and Adhesive for Stone Cultural Heritage (석조문화재 복원용 우레탄 메움제 및 접착제에 관한 연구)

  • Han, Won-Sik;Lee, Ho-Youn;Park, Gi-Jung;Hong, Tae-Kee;Wi, Koang-Chul
    • Journal of Conservation Science
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    • v.27 no.1
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    • pp.115-121
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    • 2011
  • A Urethane resin restoration material was made to be used in the restoration of stone cultural assets. The Urethane resin restoration material showed strong adhesive strength and tensile strength similar to epoxy recovery material, which had been mainly used for the recovery of stone cultural assets. The sealing property, anti-shrinking property and paint-ability of Urethane resin restoration material are also similar to existing epoxy system restoration materials. Especially, this Urethane resin restration material is expected to give permanence and continuous stability in the restoration of cultural assets made in stone by resolving the two big issues of existing epoxy recovery material, which are 'yellowing' and 'ir-reversibility'. This Urethane resin restration material had been directly applied as a filling material and adhesive and it was dissolved again. The Urethane resin, which had been used for the recovery, was able to be perfectly removed, which means that this Urethane resin recovery material has perfect reversibility. This Urethane resin restoration material also has enhanced convenience since user can adjust the working-life dependent on work environment. It is believed that this Urethane resin restoration material can also be used as a filling material or adhesive for other cultural assets made of ceramic or metal heritage, in addition to stone, since it has strong adhesive strength and tensile strength.

Study on the Development of Putty According to Epoxy Resin Hardener (Epoxy 수지 경화제에 따른 Putty 개발에 관한 연구)

  • Oh, Seung-Jun;Cheong, Da-Som;Park, Gi-Jung;Wi, Koang-Chul
    • Journal of Conservation Science
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    • v.31 no.2
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    • pp.105-114
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    • 2015
  • In this study, an advanced epoxy putty which was as a multi-purpose restoration material being used to restore missing parts in the artifact preservation treatment process was developed. For the purpose of addressing the issues including the workability issue resulting from high strength, the drooping issue resulting from long hardening time during work process and the issue of contaminating the surface of artifact resulting from stained material on tools or gloves, a property comparison was conducted with existing materials to examine the properties of restoration materials in the form of epoxy putty currently being frequently used. For the purpose of addressing the issues of existing materials and allowing the developed epoxy putty to have similar properties, two types of hardeners with different properties were selected to conduct property experiments. As for the hardeners, mercaptan type hardener and aliphatic amine type hardeners in the total of two types were selected for the development. The result showed that the two types were both in the form of paste and their hardening time of 5-10 minutes were about 3-10 times shorter than that of existing materials, thereby improving the work convenience. In terms of abrasion rate to increase workability, it was improved by about 3 times to allow effective and convenient use. As for the issue of contaminating the artifact surface during the process, white micro-balloon was added as filler to address the issue to reduce the oil ingredient to develop multi-purpose restoration material with low shrinkage & high workability in coloring, light weight and cutting force.

Estimation of Watershed Parameters and Runoff Computation Using GIS (GIS를 이용한 유역매개변수의 추정 및 유출량 산정)

  • Lee, Im-Keun;Ahn, Kyung-Soo
    • Journal of Korea Water Resources Association
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    • v.40 no.1 s.174
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    • pp.11-24
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    • 2007
  • There exist various difficulties in runoff analysis due to many ungauged basins in Korea and the runoff phenomena is also more and more complicated by the change of geologic characteristics due to the urbanization. So, we use GIS technique which is widely used in hydrologic field and cell runoff concept for the fast and effective runoff simulation. This study uses the observations of 6 stage stations in Wi-Cheon watershed and simulates the watershed parameters by using WMS model. We construct DEM by the grids which are consisted based on the criteria of minimum area according to land use. The cell runoff is estimated by an average weighted method using mean annual streamflow and mean maximum daily streamflow obtained from six stage stations. The runoff ratio at arbitrary site is estimated by conducting the direction analysis of streamflow and by removing sinkhole. We compare the simulated and observed runoffs and know that the simulated runoff shows the valid results. So, we could use the geographical information and cell runoff concept for more fast and effective runoff simulation studies.

Modern Interpretation of the Method of Learning Reflected in the Teacher-Student Relationship in On Haeng Lok by Toe-gye (퇴계 『언행록』의 사제관계에서 탐색한 학습법과 그 현대적 이해)

  • Shin, Chang-Ho;Chi, Chun-Ho;Lee, Seung-Chul;Sim, Seung-Woo
    • The Journal of Korean Philosophical History
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    • no.56
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    • pp.209-238
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    • 2018
  • The purpose of this research is to analyze characteristics of the method of education or learning reflected in the teacher-student relationship in On Haeng Lok By Toe-gye and explore their relevance to modern education. By writing various works and conversing with his students, Toe-gye devoted himself in the education of the traditional Confucian principles. Specially, he taught his students based on two Confucian educative principles, namely Shim Deuk(心得) and Goong Haeng(躬行). Judging from this, Toe-gye can be seen as someone who tries to fulfill the role of teacher as dictated in the educative principles of the Confucianism. In Confucianism, teacher is responsible for forming a well-rounded view on life in student, rather than simply transmitting knowledge. As such, the teacher was supposed to find a harmonious way to create something new based on what was inherited from the past generation and try to do his best in learning new things himself and teaching his students. These Toe-gye managed to do successfully, earning his students' trust and respect. Being moved and inspired by their teacher, the students continued their intellectual pursuit. This relationship between Toe-gye and his students can be analyzed from the perspective of education method and discussed in terms of cognitive learning and adult learning. In terms of cognitive learning, the education method reflected in the relationship is similar to potential learning, insight learning, and imitation learning. In terms of adult learning, it is similar to self-directed learning and communicative learning.!

Gu-am Kim Kyung Jang's Life and Learning (구암(龜巖) 김경장(金慶長)의 생애(生涯)와 학문(學問))

  • Yoo, Kwon Jong
    • (The)Study of the Eastern Classic
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    • no.33
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    • pp.57-96
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    • 2008
  • Kim, Kyung Jang(1597~1653) as a disciple of Jang Hyun Gwang was very famous for his virtuous conduct. But his collection of works has been published recently. There has never been any studies about his thoughts, learning and life. Therefore this study has a target to arrange the process of his life and events, and to introduce outlines of his learning. The items of this study will be as follows: The first category will be his lineage, his life, and writings. The second category will be his learning. This category has two lower categories. The first will be focused on his intention to morally accomplished self by the method of self cultivation and practise of Confucian proprieties. The second will be focused on his learning of the Change and of the Confucian proprieties. And giving attention to his 6 diagrams that have symbolized principles of the Change, this paper tries to compare these to the other scholars ones and so will explane the features of his diagrams. The most important thing in this paper is to define his learning as so called in the rhetoric of Confucianism Wi-gi-ji-hak(爲己之學), which means self organization of one's moral personality by the method of self cultivation and to distinguish the steps of his learning developed. This method has never been applied, but this is expected to become a effective method for explanation of the truth of the Confucian scholar's world of learning.

Optimization of a Highly Efficient Narrow-viewing-angle LCD for Head-mounted-display Applications (헤드마운트 디스플레이 응용을 위한 고효율 협시야각 LCD 최적화 연구)

  • Wi, Sung Hee;Kang, Min Jin;Hwang, Eui Sun;Baek, Gi Hyeon;Kim, Jin Hwan;Park, Hyeon Uk;Cheong, Byoung-Ho
    • Korean Journal of Optics and Photonics
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    • v.33 no.2
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    • pp.67-73
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    • 2022
  • In a head-mounted display (HMD) for virtual-reality applications, a narrow viewing angle is preferred to the usual, wide viewing angle because the HMD is positioned close in front of the user's eyes, and the display position is fixed. In this paper, we propose a new back-light unit (BLU) for implementing a narrow viewing angle, which is suitable for a HMD. By optimizing the scattering patterns in the light-guide-plate and inverse-prism structures, the viewing angle and correlations between structural parameters in the BLU components are analyzed with ray-tracing simulations. As a result, a double-angle inverse-prism structure incorporating the scattering patterns of a light-guide plate is chosen, which results in a 14% increase in center luminance, a 16% decrease in the vertical viewing angle, and a light efficiency of up to 70%, compared to a conventional BLU. Thus, the new BLU system is expected to be applied in a high-efficiency liquid crystal display.

A literal study on the Gu-Chang (구창의 문헌연구)

  • Jung Han Sol;Park Jong Hoon;Ryuk Sang Won;Lee Kwang Gyu
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.16 no.1
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    • pp.32-44
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    • 2002
  • Gu-Chang is a disorder characterized by recurring ulcers confined to the oral mucosa. Despite much clinical and research attention, the causes remain poorly understood. In this paper, we will compare Gu-Chang with Recurrent Aphthous Stomatitis(RAS) in order to know what is the similiarity between Gu-Chang and RAS. So we will arrange various oriental and western medical literatures which are important. As a result of arrangement of the causes, symptoms and therapys of Gu-Chang, we can conclude through the studies as follows. 1. The etiologies of Gu-chang are following. In the Sthenia syndrome, there are evil heat of external factor, heat of heart and spleen, insomnia, heat of upper warmer, stress and diet, heat of lung and heart, excessive heat of upper warmer, inappropriate food intake, heat conveyance of organ, heat of stomach merdian, moistured heat of spleen and stomach and stasis of liver energy. In the Asthenia syndrome, there are deficiency of stomach energy, deficiency of upper warmer leading to heat, deficiency of middle warmer leading to cold, deficiency of lower warmer leading to heat, deficiency of middle energy, deficiency of blood, decreased fire and deficiency of soil, yin fire of lower warmer, deficiency of heart yin, deficiency of spleen yin and deficiency of qi and blood. 2. In western medicine the causes of RAS is presumed as local, microbial, systemic, nutritional, genetic, immunologic factors. 3. Once Gu-chang is compared with RAS, in the deficiency of yin leading to hyperactivity of fire, deficiency of yin leading to floating of fire and stasis of liver energy, recurring of Gu-chang is similar to RAS. Although recurring of Gu-chang due to tripple warmer of excessive fire has no recurrance, since there are the degree of Pain, site of lesion, dysphagia etc, it is similar to major RAS. It is may be believed that Sthenia Gu-chang is similar to major RAS, shape of recurring, site of lesion, degrree of Pain and white color of Asthenia Gu-chang are similar to minor RAS, but there is no similarity concerning herpes RAS in the literatures that describe the symptoms. 4. Generally, the treatment of Gu-chang is divided into Asthenia and Sthenia Syndrome. The method of cure to Sthenia syndrome is heat cleaning and purge fire, Asthenia syndrome is nourish yin to lower and adverse rising energy and strength the middle warmer and benefit vital energy. 5. Following is the medication for Sthenia syndrome. Heat of heart and spleen is Do Jok San, Yang Gyek San, Juk Yup Suk Go Tang, evil heat of external factor is Yang Gyek San Ga Gam, Stasis of liver energy is Chong Wi Fae Dok Yum, moistured heat of spleen and stomach is Chong Gi Sam Syep Tang. The medication for Asthenia Syndrome is following. Deficiency of upper warmer leading to heat is Bo Jung Ik Gi Tang, deficiency of middle warmer leading to cold is Bu Ja Lee Jung Tang, deficiency of lower warmer leading to heat is Yuk Mi Ji Hwang Tang, deficiency of yin leading to hyperactivity of fire is Ji Baek Ji Hwang Hwan, deficiency of yin leading to floating of fire is Lee Jung Tang Ga Bu Ja Medicine for external use were Yang Suk San, Boo Wyen San, Rok Po San, Yoo Hwa San ate. 6. In western medicine, there is no specific treatment for RAS, and management strategies depend on dinical presentation and symptoms and includes antibiotics, oral rinses, glucocorticoids, immunomodulatory drugs, vitamines, analgesics, laser and antiviral agents.