• 대한화장품학회지
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• 제49권1호
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• pp.39-46
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• 2023

본 연구에서는 골든크로쿠스비늘줄기를 정제수와 에탄올 농도별로 추출을 하여 항산화 효능과 티로시나아제 저해 활성을 측정하고 기능성 화장품 원료로서의 가능성을 확인해 보았다. 추출물의 수율은 1.8 ~ 6.0%을 얻었으며, 에탄올 용매의 농도가 증가할수록 수율은 감소하였다. 항산화 효능으로 DPPH free radical (1,1-diphenyl-2-picrylhydrazyl) 소거 활성 분석한 결과 70% 에탄올 추출물 625 ㎍/mL에서 DPPH free radical 소거 활성이 91.97%로 높게 나타내었다. 총 페놀 함량에서도 447 mg/g으로 다른 추출 방법보다 높게 측정되었다. 미백 효능은 in vitro의 tyrosinase 저해 활성을 통해 확인하였다. 추출물의 에탄올 농도가 높아질수록 tyrosinase 저해 활성은 증가하였고 70%와 94.5% 에탄올 추출물에서 높은 저해 활성을 나타내었다. 따라서 골든크로쿠스비늘줄기 추출물은 항산화 및 미백 효과를 통해 화장품 소재로써의 가능성을 확인하였다.

• 융합정보논문지
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• 제12권3호
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• pp.244-251
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• 2022

본 연구에서는 와일드제라늄 추출물의 기능성화장품 원료로서 활용 가능성을 확인하였다. 라디컬 소거능으로서 DPPH, ABTS, FRAP 실험을 실시하였으며, 항산화 물질 측정으로서 폴리페놀과 플라보노이드 농도 측정을 실시하였다. 또한, 세포실험에서는 B16F10 cell과 RAW 264.7 cell을 사용하여 세포독성 실험과 미백실험, 항염 실험을 실시하였다. 실험결과 DPPH에서는 265.8 mg ascorbic acid / g의 항산화능을 나타내었으며, ABTS 실험에서는 168.5 mg ascorbic acid / g의 항산화능을 나타내었다. FRAP에서는 와일드제라늄 추출물 1 mg의 환원력과 ascorbic acid 229±9 ㎍의 환원력이 같음을 확인하였다. 폴리페놀 농도는 32.989±1.610 mg/g이었고, 플라보노이드 농도는 11.098±0.261 mg/g이었다. 세포실험에서는 실험 농도 범위에서 80% 이상의 세포가 생존하여 와일드제라늄 추출물은 낮은 독성을 가지고 있음을 확인하였다. 미백활성 실험에서는 농도에 따라 멜라닌 생성을 감소시키는 동시에 100 ㎍/mL에서 40.62±2.07%의 멜라닌 생성 억제능을 보였다. 항염활성 실험에서도 농도에 따라 염증반응을 감소시키는 동시에 100 ㎍/mL에서 27.86±2.82%의 염증 억제능을 보여 와일드제라늄 추출물의 기능성 화장품 원료로서의 가능성을 확인하였다.

• 한방안이비인후피부과학회지
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• 제16권3호
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• pp.145-163
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• 2003

Objectives : This study was performed to investigate the depigmentation effects of Aloe, Camellia sinensis and Mel. Methods : Inhibition of tyrosinase activity, melanin production & melanoma cell viability in cultured B16 melanoma cells, UV screen and cytoprotective effects on PC12 cells injured by hydrogen peroxide were measured. Results : Aloe has some inhibitory effects on tyrosinase activity, on the other hand Camellia sinensis and Mel do not have. They did not show any inhibitory effects on melanin production in melanoma cells and cytoprotective effects on PC12 cells injured by hydrogen peroxide. Aloe and Camellia sinensis have some inhibitory effects on UV screen. Conclusions : This study shows that Aloe and Camellia sinensis which were generally used for external application have some depigmentation effects. Following this, We should use them for whitening agents and the depigmentation effects of the other natural subjects which were generally used for external application should be examined.

• 동의생리병리학회지
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• 제33권6호
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• pp.349-355
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• 2019

The anti-inflammatory effects of 4'-O-methylalpinumisoflavone (OMAI) has been reported in recent years. To develop effective and safe skin whitening agents, we investigated the anti-oxidative and melanogenic effects of OMAI isolated from fruit of Maclura tricuspidata Carrière (Cudrania tricuspidata) in macrophage and melanoma cell lines. In our results, OMAI showed effective superoxide scavenging activity and suppressed production of lipopolysaccharide (LPS)-induced intracellular reactive oxygen species (ROS) in RAW264.7 cells. In addition, α-melanocyte stimulation hormone (MSH)-induced production of melanin was also reduced by OMAI in B16F10 cells. Finally, OMAI significantly inhibited tyrosinase activity in B16F10 cells. These results suggest that OMAI suppressed melanin production via scavenging reactive oxygen species and inhibition of tyrosinase activity.

• KSBB Journal
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• 제24권6호
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• pp.579-583
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• 2009

The effects of cell lytic enzyme treatment on total phenolic content, antioxidant and antityrosinase activities of lotus leaf were investigated. The dried lotus leaves were hydroyzed by cell lytic enzymes such as Promozyme, Ceremix, Pectinex, Ultraflo, Celluclast, Pentopan, Tunicase, Viscozyme at their optimum pHs (pH 5-8) at $50^{\circ}C$ for 4 hrs. Depending on the enzymes used, total phenolic compounds content was measured as $1,079-1,476{\mu}g$/mL, and antioxidant activities and whitening activities were increased by 5~10% and 20%, respectively Among the tested hydrolytic enzymes, Promozyme (pullulanase) was selected as the most suitable enzyme for the extraction of total polyphenol from lotus leaf. The optimal dosage of Promozyme were found to be 1-2% (w/w). By Promozyme treatment, total phenolic compounds content of the lotus extract significantly increased compared to the extraction without enzyme treatment.

• 대한한의학회지
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• 제38권4호
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• pp.62-81
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• 2017

Objectives: As interests in the beauty of skin is growing continuously, more people are focusing on white and clean skin. Melanin is the major factor that determines skin color. The abnormal concentration of melanin causes various skin diseases such as vitiligo, freckles, and melasma. This study investigated the inhibitory effect of Eriobotryae Folium extracts (EF) with phreatic water (PW) on the melanin synthesis. Methods: The effect of EF on melanin synthesis was evaluated by using mouse melanoma cells (B16F10). To define the mechanisms, real-time PCR and western blot were used. We also evaluated the inhibitory effects of EF and PW on melanin synthesis by using HRM-2 melanin-possessing hairless mice. After UVB irradiation, melanin differences between the skin parts that were treated and untreated with EF and PW. Levels of mRNA were measured by real-time quantitative PCR and histological analysis of the dorsal skin was conducted by hematoxylin and eosin staining. Results: EF inhibited various mechanisms of melanogenesis, and the effect was increased when combined with PW. In vitro experiments have shown that EF inhibited the expressions of tyrosinase related protein-1 (TRP-1) mRNA, tyrosinase mRNA, microphthalmia-associated transcription factor (MITF) mRNA and the tyrosinase inhibitory activation, but it stimulated the extracellular regulated kinase (ERK) mRNA expression. In vivo experiments have shown that EF prevented melanogenesis in the mice dorsal skin and inhibited TRP-1 mRNA expression. Also these effects were increased when combined with PW. Conclusions: EF and PW might be a new and effective treatment for whitening and treating pigmentation of skin.

• The Korean Journal of Physiology and Pharmacology
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• 제18권3호
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• pp.249-254
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• 2014

The purpose of this study is to characterize the effects of KHG26792 (3-(naphthalen-2-yl(propoxy)methyl)azetidine hydrochloride), a potential skin whitening agent, on melanin synthesis and identify the underlying mechanism of action. Our data showed that KHG26792 significantly reduced melanin synthesis in a dose-dependent manner. Additionally, KHG26792 downregulated microphthalmia-associated transcription factor (MITF) and tyrosinase, the rate-limiting enzyme in melanogenesis, although tyrosinase was not inhibited directly. KHG26792 activated extracellular signal-regulated kinase (ERK), whereas an ERK pathway inhibitor, PD98059, rescued KHG26792-induced hypopigmentation. These results suggest that KHG26792 decreases melanin production via ERK activation. Moreover, the hypopigmentary effects of KHG26792 were confirmed in a pigmented skin equivalent model using Cervi cornus Colla (deer antler glue), in which the color of the pigmented artificial skin became lighter after treatment with KHG26792. In summary, our findings suggest that KHG26792 is a novel skin whitening agent.

• 한국응용과학기술학회지
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• 제32권3호
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• pp.505-511
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• 2015

본 연구에서는 초임계추출 및 생물전환공정을 통하여 우방자로부터 악티게닌을 얻었다. 악티게닌은 항염증, 항인플루엔자 등의 효능이 있기 때문에 다양한 분야에서 연구되고 있다. 제조된 악티게닌은 FT-IR, $^1H$-NMR 분석결과 4-[(3,4-Dimethoxyphenyl) methyl)]dihydro-3-[(4-hydroxy-3-methoxyphenyl)methyl]-2(3H)-furanone(arctigenin)임을 확인하였고, HPLC 분석결과 95.1 % 순도임을 확인하였다. 활성물질에 대한 티로시나아제 저해 효과를 확인한 결과, 농도 의존적으로 $260{\mu}g/m{\ell}$의 농도에서 $85.06{\pm}0.9%$를 저해하였으며, 멜라닌 생성 억제 효과를 확인한 결과 $3.0{\mu}g/m{\ell}$의 농도에서 $51.1{\pm}3.7%$를 억제하는 것을 확인하였고 악티게닌이 알부틴 보다 효과가 있음을 확인하였다. 결론적으로 제조된 악티게닌은 미백화장품에 활용 가능성이 기대된다.

• 약학회지
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• 제48권6호
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• pp.323-327
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• 2004

Whitening effect, which decreases the skin pigmentation, is the one of important targets in cosmetics. This study was investigated the effects of Scirpi rhizoma on ant ioxidation and melanogenesis. S.rhizoma is a rhizome of Scirpus fluviatilis G. a perennial Cyperaceae species of wide occurrence in Asia, Europe, Africa and North America. S.rhizoma shown scavenging activities of free radicals and reactive oxygen species (ROS) with the IC50 of 638${\mu}g/ml$ against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and 21.7${\mu}g/ml$ against superoxide radicals in the xanthine/xanthine oxidase system, respectively. S.rhizoma treatment (48 h) suppressed the biosynthesis of melanin up to 27% and reduced tyrosinase activity up to 31% at 100${\mu}g/ml$ in B16 melanoma cells. S.rhizoma was also able to significantly inhibit tyrosinase and TRP-1 expres- sion in protein level. These results suggest that S.rhizoma inhibited melanin biosynthesis by regulating tyrosinase activity and expression in B16 melanoma cells. Therefore S.rhizoma may be useful as new whitening agent due to the antioxidant effect and the inhibitory effect against melanogenesis.

• KSBB Journal
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• 제28권2호
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• pp.137-145
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• 2013

In order to develop new skin whitening agents, we prepared the $CH_2Cl_2$ layer (NGC) and BuOH layer (NGB) of 75% EtOH extract of the Nelumbinis nucifera Gaertner. We measured their tyrosinase inhibitory activity in vitro and melanin synthesis inhibitory activity in B16-F1 melanoma cells. They did not show inhibitory activity against mushroom tyrosinase but showed melanin synthesis inhibitory activity in a dose-dependent manner. In a melanin synthesis inhibition assay, NGC and NGB suppressed melanin production up to 52% and 46% at a concentration of $100{\mu}g/mL$, respectively. To elucidate the mechanism of the inhibitory effects of NGC and NGB on melanogenesis, we measured the expression of melanogenesis-related proteins by western blot assay. As a result, NGC suppressed the expression of tyrosinase, tyrosinase related protein 1 (TRP-1), tyrosinase related protein 2 (TRP-2), phosphorylated cAMP responsive element binding (p-CREB) protein, and microphthalmia associated transcription factor (MITF). And NGB inhibited the protein expression of tyrosinase and MITF, but had no significant effect on TRP-1, TRP-2, and p-CREB expression. Moreover, NGB increased the expression of phosphorylated extracellular signal-regulated kinase (p-ERK). In addition, we examined the inhibitory effect on the glycosylation of tyrosinase. As a result, NGC and NGB inhibited the activity of ${\alpha}$-glucosidase in vitro and the glycosylation of tyrosinase in B16-F1 melanoma cells. From these results, we concluded that NGC and NGB could be used as active ingredients for skin whitening.