Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Antioxidant and Whitening Effect of Crocus chrysanthus (Herb.) Herb. Bulb Extracts

골든크로쿠스비늘줄기 추출물의 항산화 및 미백 효과

  • Kyung Wan Yoo (Proyou Cosmetics) ;
  • So Min Lee (Department of Biological Science, Ajou University) ;
  • So Young Jung (Department of Applied Biotechnology, Ajou University) ;
  • Hyojin Heo (Department of Applied Biotechnology, Ajou University) ;
  • Byungsun Cha (Department of Biological Science, Ajou University) ;
  • Sofia Brito (Department of Applied Biotechnology, Ajou University) ;
  • Lei Lei (Department of Biological Science, Ajou University) ;
  • Sang Hun Lee (Department of Biological Science, Ajou University) ;
  • You-Yeon Chun (Department of Biological Science, Ajou University) ;
  • Ha Hyeon Jo (Department of Biological Science, Ajou University) ;
  • Ye Ji Kim (Department of Biological Science, Ajou University) ;
  • Mi-Gi Lee (GBSA, Gyeonggido Business and Science Accelerator) ;
  • Byeong-Mun Kwak (Department of Biological Science, Ajou University) ;
  • Bum-Ho Bin (Department of Applied Biotechnology, Ajou University)
  • 유경완 ((주)프로유화장품 부설연구소) ;
  • 이소민 (아주대학교 생명과학과) ;
  • 정소영 (아주대학교 응용생명공학과) ;
  • 허효진 (아주대학교 응용생명공학과) ;
  • 차병선 (아주대학교 생명과학과) ;
  • ;
  • ;
  • 이상훈 (아주대학교 생명과학과) ;
  • 천유연 (아주대학교 생명과학과) ;
  • 조하현 (아주대학교 생명과학과) ;
  • 김예지 (아주대학교 생명과학과) ;
  • 이미기 (경기도경제과학진흥원) ;
  • 곽병문 (아주대학교 생명과학과) ;
  • 빈범호 (아주대학교 응용생명공학과)
  • Received : 2022.10.26
  • Accepted : 2023.01.04
  • Published : 2023.03.30
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Abstract

In this study, Crocus chrysanthus (Herb.) Herb. bulb extracts were extracted by purified water and ethanol, and their antioxidant and tyrosinase inhibitory activity were measured to see the possibility as a cosmetic material. The yield of the extracts was 1.8% to 6.0%, and the yield decreased as the ethanol concentration increased. DPPH free radical (1,1-diphenyl-2-picrylhydrazyl) scavenging activity was high at 91.97% at 625 ㎍/mL of 70% ethanol extract. The total phenol content was also measured at 447 mg/g, higher than other extraction methods. The whitening effect was confirmed through in vitro tyrosinase inhibitory activity. As the ethanol concentration of the extracts increased, the tyrosinase inhibitory activity increased, and 70% and 94.5% ethanol extracts showed high inhibitory activity. Therefore, the Crocus chrysanthus (Herb.) Herb. bulb extracts confirmed its potential as a cosmetic material through antioxidant and whitening effects.

본 연구에서는 골든크로쿠스비늘줄기를 정제수와 에탄올 농도별로 추출을 하여 항산화 효능과 티로시나아제 저해 활성을 측정하고 기능성 화장품 원료로서의 가능성을 확인해 보았다. 추출물의 수율은 1.8 ~ 6.0%을 얻었으며, 에탄올 용매의 농도가 증가할수록 수율은 감소하였다. 항산화 효능으로 DPPH free radical (1,1-diphenyl-2-picrylhydrazyl) 소거 활성 분석한 결과 70% 에탄올 추출물 625 ㎍/mL에서 DPPH free radical 소거 활성이 91.97%로 높게 나타내었다. 총 페놀 함량에서도 447 mg/g으로 다른 추출 방법보다 높게 측정되었다. 미백 효능은 in vitro의 tyrosinase 저해 활성을 통해 확인하였다. 추출물의 에탄올 농도가 높아질수록 tyrosinase 저해 활성은 증가하였고 70%와 94.5% 에탄올 추출물에서 높은 저해 활성을 나타내었다. 따라서 골든크로쿠스비늘줄기 추출물은 항산화 및 미백 효과를 통해 화장품 소재로써의 가능성을 확인하였다.

Keywords

References

  1. H. J. Son, W. Whang, and E. J. Lee, The antioxidant activities and whitening effect Prunus domesica L. extracts, J. Soc. Cosmet. Sci. Korea, 23(4), 688 (2017).
  2. N. Tsuji, S. Moriwaki, Y. Suzuki, Y. Takema, and G. Imokawa, The role of elastases secreted by f ibroblasts in wrinkle formation: implication through selective inhibition of elastase activity, Photochem Photobiol., 74(2), 283 (2001).
  3. V. Afonso, R. Champy, D. Mitrovic, P. Collin, and A. Lomri, Reactive oxygen species and superoxide dismutases role in joint diseases, Joint Bone Spine, 74(4), 324 (2007).
  4. O. H. Yoon, B. Jeong, E. Kim, and Y. Jeong, Chemical composition and antioxidant activities of Prunus salicina Formosa produced in Gimcheon, J. Korean. Soc. Food. Sci, Nutr., 40(3), 379 (2011).
  5. N. Puizina-Ivic, Skin aging, Acta Dermatoven., 17(2), 47 (2008).
  6. A. Kammeyer and R. M. Luiten, Oxidation events and skin aging, Ageing Res. Rev., 21, 16 (2015).
  7. M. Yaar and B. A. Gilchrest, Photoageing: mechanism, prevention and therapy, Br. J. Dermatol., 157(5), 874 (2007).
  8. H. J. Yang, E. H. Kim, and S. N. Park, Antioxidative activity and component analysis of Psidium guajava leaf extracts, J. Soc. Cosmet. Sci. Korea, 34(3), 233 (2008).
  9. H. U. Simon, A. Haj-Yehia, and F. Levi-Schaffer, Role of reactive oxygen species (ROS) in apoptosis induction, Apoptosis, 5(5), 415 (2000).
  10. G. M. Williams, C. X. Wang, and M. J. Iatropoulos, Toxicity studies of butylated hydroxyanisole and butylated hydroxytoluene. II. Chronic feeding studies, Food Chem. Toxicol, 28(12), 799 (1990).
  11. A. L. Branen, Toxicology, and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene, J Am Oil Chem Soc., 52(2), 59 (1975).
  12. H. M. Seog, M. S. Seo, H. M. Kim, M. S. Ahn, and Y. T. Lee, Antioxidative activity of barley polyphenol extract (BPE) separated from pearling by-products, Korean J. Food Sci. Technol., 34(5), 59 (2002).
  13. J. Cabanes, S. Chazarra, and F. Garcia-Carmona, Kojic acid, a cosmetic skin whitening agent, is a slow-binding inhibitor of catecholase activity of tyrosinase, J. Pharm. Pharmacol, 46(12), 982 (1994).
  14. E. J. Kwon, H. J. Park, M. M. Kim, K. R. Lee, I. Hong, D. G. Lee, and Y. H. Oh, Ef f ect of Ulmus macrocapa ethanolic extracts on anti-oxidant activity and melanin synthesis in B16F1 cells, J. Life. Sci., 24(9), 946 (2014).
  15. J. J. Ahn, T. Y. Hwang, and H. S. Kim, Study on the physiological activities of Cleyera japonica extract, Korean J. Plant Res., 28(2), 153 (2015).
  16. E. J. Seo, E. S. Hong, M. H. Choi, K. S. Kim, and S. J. Lee, The antioxidant and skin whitening effect of Artemisia iwayomogi extracts, Korean J. Food Sci. technol., 44(1), 89 (2012).
  17. T. Aburjai, M. Hudaib, R. Tayyem, M. Yousef, and M. Qishawi, Ethnopharmacological survey of medicinal herbs in Jordan, the Ajloun Heights region, J. Ethnopharmacol., 110(2), 294 (2007).
  18. R. Norbaek, J. K. Nielsen, and T. Kondo, Flavonoids from flowers of two Crocus chrysanthus-biflorus cultivars:"Eye-catcher" and "Spring Pearl" (Iridaceae), Phytochemistry, 51(8), 1139 (1999).
  19. G. Zengin, M. Z. Aumeeruddy, A. Diuzheva, J. Jeko, Z. Cziaky, A. Yildiztugay, E. Yildiztugay, and M. F. Mahomoodally, A comprehensive appraisal on Crocus chrysanthus (Herb.) Herb. flower extracts with HPLC-MS/MS profiles, antioxidant and enzyme inhibitory properties, J Pharm Biomed Anal., 164, 581 (2019).
  20. Y. S. Han and E. S. Jung, A study of correlation between antioxidant activity and whitening effect of plant extract, Kor. J. Aesthet. Cosmetol., 1(1), 11 (2003).
  21. Y. K. Park, S. H. Choi, S. H. Kim, J. G. Han, and H. G. Chung, Changes in antioxidant activity, total phenolics and vitamin C content during fruit ripening in Rubus occidentalis, Korea J. Plant Res., 20(5), 461 (2007).
  22. B. H. Kang and M. J. Ryu, Physiological activities of the Neem and the comfrey extracts as cosmetic ingredients, Asian J. Beauty Cosmetol., 19(2), 223 (2021).
  23. S. Y. Kim, C. R. Kim, H. M. Kim, M. Kong, J. H. Lee, H. J. Lee, M. S Lim, N. R, Jo, and S. N. Park, Antioxidant activity and whitening effect of Cedrela sinensis A. Juss shoots extracts, J. Soc. Cosmet. Sci. Korea, 36(3), 175 (2010).
  24. M. S. Blois, Antioxidant Determinations by the use of a stable free radical, Nature, 181, 1199 (1958).
  25. V. L. Singleton and J. A. Rossi, Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents, J. Enol. Vitic., 16(3), 144 (1965).
  26. J. Y. Cha, J. J. Jeong, Y. T. Kim, W. S. Seo, H. J. Yang, J. S. Kim, and Y. S. Lee, Detection of chemical characteristics in hamcho (Salicornia herbacea) according to harvest periods, J. Life Sci., 16, 683 (2006).
  27. J. Y. Kim, Y. S, Maeng, and K. Y. Lee., Antioxidative effects of soybean extracts by using various solvents, Korean J. Food Sci. Technol., 27(5), 635 (1995).
  28. J. Y. Cha, H. Y. Ahn, K. E. Eom, B. K. Park, B. S. Jun, and Y. S. Cho, Antioxidative activity of Aralia elata shoot and leaf extracts, J. Life Sci., 19(5), 652 (2009).
  29. K. A. Lee, K. T. Kim, H. J. Kim, M. S. Chung, P. S. Chang, H. Park, and H. D. Pai, Antioxidant activities of onion (Allium cepa L.) peel extracts produced by ethanol, hot water, and subcritical water extraction, Food Sci. Biotechnol., 23(2), 615 (2014).
  30. Z. Mohammedi, and F. A. Atik, Impact of solvent extraction type on total polyphenols content and biological activity from Tamarix aphylla (L.) Karst, Int. J. Pharma Bio Sci., 2(1), 609 (2011).
  31. C. Y. Lee, K. M. Kim, and H. S. Son, Optimal extraction conditions to produce rosemary extracts with higher phenolic content and antioxidant activity, Korean J. Food Sci. technol., 45(4), 501 (2013).
  32. S. K. Choi, Q. M. Yu, E.J. Lim, and J. S. Seo, The effects of extraction conditions on the antioxidative effects of extracts from campbell early and muscat bailey a grapevine leaves, J. Korean Soc. Food Sci. Nutr., 42(2), 168 (2013).
  33. C. Rice-Evans, N. Miller, and G. Paganga, Antioxidant properties of phenolic compounds, Trends Plant Sci, 2(4), 152 (1997).
  34. S. O. Lee, H. J. Lee, M. H. Yu, H. G Im, and I. S Lee, Total polyphenol contents and antioxidant activities of methanol extracts from vegetables produced in Ullung island, Korean J. Food Sci. Technol,. 37(2), 233 (2005).
  35. H. J. Jeong, S. B. Park, S. Kim, and H. K. Kim, Total polyphenol content and antioxidative activity of wild grape (Vitis coignetiae) extracts depending on ethanol concentration, J Korean Soc Food Sci Nutr., 36(12), 1491 (2007).
  36. I. Han and J. H. Kim, Antioxidant and physiological activities of water and ethanol extracts of diverse parts of welsh onion, J Korean Soc Food Sci Nutr., 46(4), 426 (2017).
  37. S. B. Han, S. K. Kwon, B. J. Kong, K. J. Kim, and S. N. Park, Antioxidative effect and tyrosinase inhibitory activity of the unripened fruit extract of Rubus coreanus Miquel, J. Soc. Cosmet. Sci. Korea, 39(4), 295 (2013).
  38. M. J. Choi, Skin whitening efficacy of mixed extracts of Crassostrea gigas, Trichosanthes cucumeroides, Angelica dahurica and Asarum sieboldii, Asian J. Beauty. Cosmetol., 20(2), 157 (2022).
  39. I. H. Kim and J. H. Lee, Skin whitening and anti-wrinkle ef f ects of chambirum (Amaranthus mangostanus), Asian J. Beauty. Cosmetol., 20(1), 21 (2022).
  40. Y. Jin, S. Y. Ahn, E. S. Hong, G. H. Li, E. K. Kim, and K. H. Row, Extraction of whitening agents from natural plants and whitening effect, J. Korean Ind. Eng. Chem, 16(3), 348 (2005).
  41. Y. S. Han and E. S. Jung, A study of correlation between antioxidant activity and whitening effect of plant extracts, Asian J. Beauty. Cosmetol., 1(1), 11 (2003).