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Effects of the Sciripi rhizoma on Antioxidation and Melanogenesis  

Lee, Kyung-Eun (R&D Center, Hanbul Cosmetics Co. Ltd)
Sim, Gwan-Sub (R&D Center, Hanbul Cosmetics Co. Ltd)
Kim, Jin-Hwa (R&D Center, Hanbul Cosmetics Co. Ltd)
Park, Sung-Min (R&D Center, Hanbul Cosmetics Co. Ltd)
Lee, Bum-Chun (R&D Center, Hanbul Cosmetics Co. Ltd)
Yun, Yeo-Pyo (College of Pharmacy, Chungbuk National University)
Zhang, Yong He (Department of Pharmacology, School of Basic Medical Science, Peking University)
Pyo, Hyeong-Bae (R&D Center, Hanbul Cosmetics Co. Ltd)
Publication Information
YAKHAK HOEJI / v.48, no.6, 2004 , pp. 323-327 More about this Journal
Abstract
Whitening effect, which decreases the skin pigmentation, is the one of important targets in cosmetics. This study was investigated the effects of Scirpi rhizoma on ant ioxidation and melanogenesis. S.rhizoma is a rhizome of Scirpus fluviatilis G. a perennial Cyperaceae species of wide occurrence in Asia, Europe, Africa and North America. S.rhizoma shown scavenging activities of free radicals and reactive oxygen species (ROS) with the IC50 of 638${\mu}g/ml$ against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and 21.7${\mu}g/ml$ against superoxide radicals in the xanthine/xanthine oxidase system, respectively. S.rhizoma treatment (48 h) suppressed the biosynthesis of melanin up to 27% and reduced tyrosinase activity up to 31% at 100${\mu}g/ml$ in B16 melanoma cells. S.rhizoma was also able to significantly inhibit tyrosinase and TRP-1 expres- sion in protein level. These results suggest that S.rhizoma inhibited melanin biosynthesis by regulating tyrosinase activity and expression in B16 melanoma cells. Therefore S.rhizoma may be useful as new whitening agent due to the antioxidant effect and the inhibitory effect against melanogenesis.
Keywords
Scirpi rhizoma; antioxidation; melanogenesis; tyrosinase;
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1 Cabanes, J., Chazarra, S. and Garcia-Carmona, F. : Kojic acid, a cosmetic skin whitening agent, is a slow-binding inhibitor of catecholase activity of tyrosinase. J. Pharm Pharmacol. 46(12), 982 (1994)
2 Ryu, K. Y., Kang, W. S., Kim, Y. H., Jang, H. D., Hong, J. T., Yoo, H. S. and Yun, Y. P. : Antioxidative effects of the rhizoma of Rhodiola sa halinensis. Yakhak Hoeji. 42(3), 312 (1998)
3 Lee, S. H., Park, J. S., Kim, S. Y., Kim, J. J. and Chung, S. R. : Isolation of Inhibitory components on tyrosinase activity from the back of paeonia moutan. Yakhak Hoeji. 42(4), 353 (1998)
4 Mosmann, T. : Rapid colorimetric assay for the cellular growth and survival : application to proliferation and cytotoxic assay. J. Immun. Methos. 65, 55 (1983)
5 Hearing, V. J. and Tsukamoto, K. : Biochemical control of melanoenesis and melanosornal organization. J. Invest Dermatol. 4, 24 (1999)   DOI   ScienceOn
6 Higuchi, M., Miura, Y., Boohena, J., Kinoshita, Y.,Yamamamoto, Y., Yushimura, I. and Yamaha, Y. : Inhibition of tyrosinase activity by cultured lichen tissues and bionts. Planta. Med. 59, 253 (1993)   DOI   ScienceOn
7 Blois, M. S. : Antioxidation determination by the use of a stable free radical. Nature 181, 1199 (1958)   DOI   ScienceOn
8 Pawelk, J. : Melanoma cells in culture. Methods Enzymol. 58, 564 (1978)
9 Yasunobu, O., Tomoko, K., Yuri, O., Hitoshi, M., Yoshiko, K., Yoko, F., Masamitsu, I., Ytaka, Y., Yoshitane, K. and Hiromu, S. : Development of a novel zinc complex as whitening agent in a new concept. ASCS. 6th, 69 (2003)
10 Kuppusamy, P. and Zweier, J. L. : Characterization of free radical generation by xanthine oxidase. J. BioI. Chem. 264, 9880 (1989)
11 Masaki, H., Sakaki, S., Atsumi, T. and Sakurai, H. : Active-oxygen scavengmg activity of plant extracts. BioI. Pharm. Bull. 18, 162 (1995)
12 Pomerantz, S. H. : Separation, purification, and properties of two tyrosinases from hamster melanoma. J. BioI. Chem. 238, 2351 (1963).
13 Hill, H. Z., Li, w., Xin, P. and Michell, D. L. : Melanin : a two edged sword? Pigment Cell Res. 10(3), 158 (1997)
14 Park, J. H., Shin, Y. G., Baek, S. K., Lee, U. K., Chung, H. and Park, Y. I. : Tyrosinase inhibition activity of some herbal drugs. Yakhak Hoeji. 41(4), 518 (1997)
15 Kitahara, A., Matsumoto, U., Ueda, H. and Ueoka, R. : Aremarkable antioxidation effect of natural phenol derivatives on the autoxidation of ${\gamma}$-irradiated methyl linolate. Chem. Pharm. Bull. 40, 2208 (1992)
16 Veronique del Marmol and Friedrich Beermann. : Tyrosnase and related protein in mammalian pigmentation. FEBS Letters 381, 165 (1996)
17 Hatano, T. : Constituents of natural medicines with scavenging effects on active oxygen species-Tannins and related polyphenols. Natural Medicines. 49, 357 (1995)
18 Furuno, K., Akasako, T. and Sugihara, N. : The contribution of the pyrogallol moiety to the superoxide radical scavenging activity of flavonoids. Biol. Pharm. Bull. 25, 19 (2002)   DOI   ScienceOn