• 전기의세계
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• v.65 no.6
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• pp.12-18
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• 2016

• Journal of Korean Medical classics
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• v.20 no.2
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• pp.125-135
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• 2007

Eum Syndrom include symptoms which is because by the cold thingsand by the infection of SamEum of TaeEum, SoEum, GualEum in Treatise On Exogenous Febrile Diseases(傷寒論). After Wang HoGo, many medical people had proceed the research of Eum Syndrom, but recently, we have almost never or no nothing research about that. So, I want to make modern base of Eum syndrom, and researched mainly for the "YinZhengLueLi". That can be summarized like below. Eum Syndrom shows firstly red face, tremor, waist-and-leg heaviness, lastly body heaviness, fatigue, narcolepsy, congestion of the pupils because of from exogenous attack of wind-cold, impairment of spleen due to Cold things, and dew and mist and rain and damp's invation by mouth and nose, greedy of sexual desire, So, in the diagnosis of Eum Syndrom, we have to look over precisely the color and pulse, especially, by pulse. We can know that, he used the prescription which are have heating kidney function, Byuklyuksan, Jungyangsan, Huahamsan, Huiyangdan, Baneumdan etc, and the prescription which are have strengthening spleen and kidney, Bujasan, Yukgyesan, Bakchulsan etc. So, we can know that he was very interested in deficiency and cold of kidney's function. While, he newly made the prescriptions of Sinchultang, Bakchultang, Huanggitang, Jujunghuan, and he used various prescriptions.

• The Journal of Korean Medical History
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• v.20 no.1
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• pp.7-12
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• 2007

Wang Hao Gu is a famous Chinese medical scientist of the 13 century. He studied the oldest classic in China, Huangjaenaekyung, and also studied Zhangzhongjing's medical documents. He was influenced by contemporary medical scientists Zhang Yuan Su and Li Dong Yuan, and came up with an original medical theory Yinzheng. Although he is of considerable importance in the history of medicine, not many studies on him were done in Korea although Korean medicine was influenced by Chinese medicine. This study focuses on the study of his insight on medical stuffs and theory of Shanghan.

• The Journal of Pediatrics of Korean Medicine
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• v.22 no.2
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• pp.187-204
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• 2008

Objectives : Myopia is a disease which can be easily found in the child and it considers as a prevalent disease. This study has a reference of the literatures of oriental medicine on the myopia to investigate treatment principles of the myopia. Methods : This study is based on the literatures of oriental medicine. Results : According to the literatures of oriental medicine, there were two opposite opinions those could cause myopia. One was 'lack of Yinqi(陰氣)', the other was 'lack of Yangqi(陽氣)'. Originally many scholars thought that myopia was caused by laking of Yinqi(陰氣). The representative scholar was LiGao, and his work is Dongwonsunsangsihyobang(東垣先生試效方). But, some scholars insisted the opposite opinions, and the next generations accepted this opinion. The representative scholar was WangHaoGu(王好古), his work was Chasanangi(此事難知). Euihakgangmok(醫學綱目) published conversely the opinion of LiGao. Therefore, after ages appreciated that LiGao and WangHaoGu(王好古) insisted the same opinions-the cause of the myopia was lack of Yangqi(陽氣). So the most latest generation scholars knew that the cause of myopia was lack of Yangqi(陽氣). Conclusions : Historically many scholars insisted that myopia was caused by lack of 'Yangqi(陽氣)'. But that is the result of false transmission through the generation.

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.8
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• pp.1057-1064
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• 2013

p70 ribosomal S6 kinase (p70S6K) can integrate nutrient and growth factor signals to promote cell growth and survival. We report our molecular characterization of the complementary DNA (cDNA) that encodes the goat p70S6K gene 40S ribosomal S6 kinase 1 (S6K1) (GenBank accession GU144017) and its 3' noncoding sequence in Inner Mongolia Cashmere goats (Capra hircus). Goat S6K1 cDNA was 2,272 bp and include an open reading frame (ORF) of 1,578 bp, corresponding to a polypeptide of 525 amino acids, and a 694-residue 3' noncoding sequence with a polyadenylation signal at nucleotides 2,218 to 2,223. The relative abundance of S6K1 mRNA was measured by real-time PCR in 6 tissues, and p70S6K expression was examined by immunohistochemistry in heart and testis. The phosphorylation of p70S6K is regulated by mitogen-activated protein kinase (MAPK) signaling in fetal fibroblasts.

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.11
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• pp.1644-1650
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• 2013

Ribosomal protein (rp) S6 is the substrate of ribosomal protein S6K (S6 kinase) and is involved in protein synthesis by mTOR/S6K/S6 signaling pathway. Some S6 cDNA have been cloned in mammals in recent years but has not been identified in the goat. To facilitate such studies, we cloned the cDNA encoding Cashmere goat (Capra hircus) S6 (GenBank accession GU131122) and then detected mRNA expression in seven tissues by real time PCR and protein expression in testis tissue by immunohistochemisty. Sequence analysis indicated that the obtained goat S6 was a 808 bp product, including a 3' untranslated region of 58 bp and an open reading frame of 750 bp which predicted a protein of 249 amino acids. The predicted amino acid sequence was highly homologous to cattle, human, mouse and rat S6. Expression analysis indicated S6 mRNA was expressed extensively in detected tissues and S6 protein was expressed in testis tissue.

• Journal of Microbiology and Biotechnology
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• v.26 no.6
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• pp.1077-1086
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• 2016

Glycerol dehydrogenases (GlyDHs) are essential for glycerol metabolism in vivo, catalyzing its reversible reduction to 1,3-dihydroxypropranone (DHA). The gldA gene encoding a putative GlyDH was cloned from Thermoanaerobacterium thermosaccharolyticum DSM 571 (TtGlyDH) and expressed in Escherichia coli. The presence of Mn²⁺ enhanced its enzymatic activity by 79.5%. Three highly conserved residues (Asp¹⁷¹, His²⁵⁴, and His²⁷¹) in TtGlyDH were associated with metal ion binding. Based on an investigation of glycerol oxidation and DHA reduction, TtGlyDH showed maximum activity towards glycerol at 60℃ and pH 8.0 and towards DHA at 60℃ and pH 6.0. DHA reduction was the dominant reaction, with a lower K_m(DHA) of 1.08 ± 0.13 mM and V_max of 0.0053 ± 0.0001 mM/s, compared with glycerol oxidation, with a K_m(glycerol) of 30.29 ± 3.42 mM and V_max of 0.042 ± 0.002 mM/s. TtGlyDH had an apparent activation energy of 312.94 kJ/mol. The recombinant TtGlyDH was thermostable, maintaining 65% of its activity after a 2-h incubation at 60℃. Molecular modeling and site-directed mutagenesis analyses demonstrated that TtGlyDH had an atypical dinucleotide binding motif (GGG motif) and a basic residue Arg⁴³, both related to dinucleotide binding.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.10
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• pp.5849-5854
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• 2013

Background: Apoptosis may be induced after Bcl-2 expression is inhibited in proliferative cancer cells. This study focused on the effect of autophagy activation by ABT737 on anti-tumor effects of epirubicin. Methods: Cytotoxic effects of ABT737 on the HepG2 liver cancer cell line were assessed by MTT assay and cell apoptosis through flow cytometry. Mitochondrial membrane potential was measured by fluorescence microscopy. Monodansylcadaverin (MDC) staining was used to detect activation of autophagy. Expression of p53, p62, LC3, and Beclin1, apoptotic or autophagy related proteins, was detected by Western blotting. Results: ABT737 and epirubicin induced growth inhibition in HepG2 cells in a dose- and time-dependent manner. Both ABT737 and epirubicin alone could induce cell apoptosis with a reduction in mitochondrial membrane potential as well as increased apoptotic protein expression. Further increase of apoptosis was detected when HepG2 cells were co-treated with ABT373 and epirubicin. Furthermore, our results demonstrated that ABT373 or epirubicin ccould activate cell autophagy with elevated autophagosome formation, increased expression of autophagy related proteins and LC3 fluorescent puncta. Conclusions: ABT737 influences cancer cells through both apoptotic and autophagic mechanisms, and ABT737 may enhance the effects of epirubicin on HepG2 cells by activating autophagy and inducing apoptosis.

• Korean Journal of Materials Research
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• v.30 no.12
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• pp.641-649
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• 2020

A facile microwave assisted solvothermal process is designed for fabricating SnS nanoparticles decorated on graphene nanosheet, which used as visible light driven photocatalyst. Some typical characterization techniques such as XRD, FT-IR, SEM with EDX analysis, and TEM and BET analysis are used to analyse the physical characteristics of as-prepared samples. Spherical SnS nanoparticles are uniformly dispersed on the surface of graphene nanosheet due to ammonia, which can prevent the aggregation of graphene oxide. Meanwhile, microwave radiation provides fast energy that promotes the formation of spherical SnS nanoparticles within a short time. The visible light photocatalytic activity of as-prepared SnS-GR nanocomposites is analysed through photodegradation efficiency of methylene blue with high concentration. According to the higher photocatalytic property, the as-prepared SnS-GR nanocomposites can be expected to be an efficient visible light driven photocatalyst. After five cycles for decolorization, the rate decreases from 87 % to 78 % (about 9 %). It is obvious that the photocatalytic activity of SnS-GR nanocomposite has good repeatability.

• Structural Engineering and Mechanics
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• v.81 no.5
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• pp.539-550
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• 2022

A detailed experimental program was conducted to investigate the flexural behavior of ultra high performance concrete (UHPC) beams reinforced with high strength steel (HSS) rebars with a specified yield strength of 600 MPa via direct tensile test and monotonic four-point bending test. First, two sets of direct tensile test specimens, with the same reinforcement ratio but different yield strength of reinforcement, were fabricated and tested. Subsequently, six simply supported beams, including two plain UHPC beams and four reinforced UHPC beams, were prepared and tested under four-point bending load. The results showed that the balanced-reinforced UHPC beams reinforced with HSS rebars could improve the ultimate load-bearing capacity, deformation capacity, ductility properties, etc. more effectively owing to interaction between high strength of HSS rebar and strain-hardening characteristic of UHPC. In addition, the UHPC with steel rebars kept strain compatibility prior to the yielding of the steel rebar, further satisfied the plane-section assumption. Most importantly, the crack pattern of the UHPC beam reinforced with HSS rebars was prone to transform from single main crack failure corresponding to the normal-strength steel, to multiple main cracks failure under the condition of balanced-reinforced failure, which validated by the conclusion of direct tensile tests cooperated with acoustic emission (AE) source locating technique as well.