Browse > Article
http://dx.doi.org/10.5713/ajas.2012.12710

Molecular Characterization and Expression Analysis of S6K1 in Cashmere Goats (Capra hircus)  

Wu, Manlin (College of Life Science, Inner Mongolia University)
Bao, Wenlei (College of Life Science, Inner Mongolia University)
Hao, Xiyan (College of Life Science, Inner Mongolia University)
Zheng, Xu (College of Life Science, Inner Mongolia University)
Wang, Yanfeng (College of Life Science, Inner Mongolia University)
Wang, Zhigang (College of Life Science, Inner Mongolia University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.26, no.8, 2013 , pp. 1057-1064 More about this Journal
Abstract
p70 ribosomal S6 kinase (p70S6K) can integrate nutrient and growth factor signals to promote cell growth and survival. We report our molecular characterization of the complementary DNA (cDNA) that encodes the goat p70S6K gene 40S ribosomal S6 kinase 1 (S6K1) (GenBank accession GU144017) and its 3' noncoding sequence in Inner Mongolia Cashmere goats (Capra hircus). Goat S6K1 cDNA was 2,272 bp and include an open reading frame (ORF) of 1,578 bp, corresponding to a polypeptide of 525 amino acids, and a 694-residue 3' noncoding sequence with a polyadenylation signal at nucleotides 2,218 to 2,223. The relative abundance of S6K1 mRNA was measured by real-time PCR in 6 tissues, and p70S6K expression was examined by immunohistochemistry in heart and testis. The phosphorylation of p70S6K is regulated by mitogen-activated protein kinase (MAPK) signaling in fetal fibroblasts.
Keywords
Inner Mongolia Cashmere Goat; S6K1; Expression Pattern; U0126;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Chung, J., C. J. Kuo, G. R. Crabtree, and J. Blenis. 1992. Rapamycin-FKBP specifically blocks growth-dependent activation of and signaling by the 70 kd S6 protein kinases. Cell 69:1227-1236.   DOI   ScienceOn
2 Coffer, P. J., and J. R. Woodgett. 1994. Differential subcellular localization of two isoforms of p70 S6 protein kinase. Biochem. Biophys. Res. Commun. 198:780-786.   DOI   ScienceOn
3 Duvel, K., J. L. Yecies, S. Menon, P. Raman, A. I. Lipovsky, A. L. Souza, E. Triantafellow, Q. Ma, R. Gorski, and S. Cleaver. 2010. Activation of a metabolic gene regulatory network downstream of mTOR complex 1. Mol.cell 39:171-183.   DOI   ScienceOn
4 Dibble, C. C., J. M. Asara, and B. D. Manning. 2009. Characterization of Rictor phosphorylation sites reveals direct regulation of mTOR complex 2 by S6K1. Mol. Cell. Biol. 29:5657-5670.   DOI   ScienceOn
5 Duchene, S., E. Audouin, S. Crochet, M. J. Duclos, J. Dupont, and S. Tesseraud. 2008. Involvement of the ERK1/2 MAPK pathway in insulin-induced S6K1 activation in avian cells. Domest. Anim. Endocrinol. 34:63-73.   DOI   ScienceOn
6 Favata, M. F., K. Y. Horiuchi, E. J. Manos, A. J. Daulerio, D. A. Stradley, W. S. Feeser, D. E. Van Dyk, W. J. Pitts, R. A. Earl, and F. Hobbs. 1998. Identification of a novel inhibitor of mitogen-activated protein kinase kinase. J. Biol. Chem. 273:18623-18632.   DOI   ScienceOn
7 Fenton, T. R., and I. T. Gout. 2011. Functions and regulation of the 70kDa ribosomal S6 kinases. Int. J. Biochem. Cell Biol. 43:47-59.   DOI   ScienceOn
8 Gout, I., T. Minami, K. Hara, Y. Tsujishita, V. Filonenko, M. D. Waterfield, and K. Yonezawa. 1998. Molecular cloning and characterization of a novel p70 S6 kinase, p70 S6 kinase ${\beta}$ containing a proline-rich region. J. Biol.Chem. 273:30061-30064.   DOI
9 Grove, J. R., P. Banerjee, A. Balasubramanyam, P. Coffer, D. Price, J. Avruch, and J. Woodgett. 1991. Cloning and expression of two human p70 S6 kinase polypeptides differing only at their amino termini. Mol. Cell. Biol. 11:5541-5550.
10 Li, S., W. Ogawa, A. Emi, K. Hayashi, Y. Senga, K. Nomura, K. Hara, D. Yu, and M. Kasuga. 2011. Role of S6K1 in regulation of SREBP1c expression in the liver. Biochem. Biophys. Res. Commun. 412:197-202.   DOI   ScienceOn
11 Medeiros, C., M. J. Frederico, G. da Luz, J. R. Pauli, A. S. Silva, R. A. Pinho, L. A. Velloso, E. R. Ropelle, and C. T. De Souza. 2011. Exercise training reduces insulin resistance and upregulates the mTOR/p70S6k pathway in cardiac muscle of diet-induced obesity rats. J. Cell. Physiol. 226:666-674.   DOI   ScienceOn
12 Melnik, B. C. 2012. Leucine signaling in the pathogenesis of type 2 diabetes and obesity. World J. Diabetes 3:38-53.   DOI   ScienceOn
13 Moreno-Navarrete, J. M., F. Ortega, M. A. Sanchez-Garrido, M. Sabater, W. Ricart, A. Zorzano, M. Tena-Sempere, and J. M. Fernandez-Real. 2013. Phosphorylated S6K1 (Thr389) is a molecular adipose tissue marker of altered glucose tolerance. J. Nutr. Biochem. 24:32-38. doi:10.1016/j.jnutbio.2012.01.005   DOI   ScienceOn
14 Nawroth, R., F. Stellwagen, W. A. Schulz, R. Stoehr, A. Hartmann, B. J. Krause, J. E. Gschwend, and M. Retz. 2011. S6K1 and 4E-BP1 are independent regulated and control cellular growth in bladder cancer. PLoS One 6:e27509. doi: 10.1371/ journal.pone.0027509   DOI
15 Proud, C. G. 2002. Regulation of mammalian translation factors by nutrients. Eur. J. Biochem. 269:5338-5349.   DOI   ScienceOn
16 Pullen, N., P. B. Dennis, M. Andjelkovic, A. Dufner, S. C. Kozma, B. A. Hemmings, and G. Thomas. 1998. Phosphorylation and activation of p70s6k by PDK1. Science 279:707-710.   DOI   ScienceOn
17 Ragan, T. J., D. B. Ross, M. M. Keshwani, and T. K. Harris. 2008. Expression, purification, and characterization of a structurally disordered and functional C-terminal autoinhibitory domain (AID) of the 70 kDa 40S ribosomal protein S6 kinase-1 (S6K1). Protein Expr. Purif. 57:271-279.   DOI   ScienceOn
18 Rosner, M., and M. Hengstschlager. 2011. Nucleocytoplasmic localization of p70 S6K1, but not of its isoforms p85 and p31, is regulated by TSC2/mTOR. pncogene 30:4509-4522.
19 Reinhard, C., A. Fernandez, N. Lamb, and G. Thomas. 1994. Nuclear localization of p85s6k: functional requirement for entry into S phase. EMBO J. 13:1557-1565.
20 Rolfe, M., L. E. McLEOD, P. F. Pratt, and C. G. Proud,. 2005. Activation of protein synthesis in cardiomyocytes by the hypertrophic agent phenylephrine requires the activation of ERK and involves phosphorylation of tuberous sclerosis complex 2 (TSC2). Biochem. J. 388:973-984.   DOI   ScienceOn
21 Rosner, M., K. Schipany, and M. Hengstschlager. 2012. p70 S6K1 nuclear localization depends on its mTOR-mediated phosphorylation at T389, but not on its kinase activity towards S6. Amino Acids 42:2251-2256.   DOI   ScienceOn
22 Saitoh, M., N. Pullen, P. Brennan, D. Cantrell, P. B. Dennis, and G. Thomas. 2002. Regulation of an activated S6 kinase 1 variant reveals a novel mammalian target of rapamycin phosphorylation site. J.Biol. Chem. 277:20104-20112.   DOI   ScienceOn
23 Schwede, T., J. Kopp, N. Guex, and M. C. Peitsch. 2003. SWISS-MODEL: an automated protein homology-modeling server. Nucleic Acids Res. 31:3381-3385.   DOI   ScienceOn
24 Selman, C., J. Tullet, D. Wieser, E. Irvine, S. J. Lingard, A. I. Choudhury, M. Claret, H. Al-Qassab, D. Carmignac, and F. Ramadani. 2009. Ribosomal Protein S6 kinase 1 signaling regulates mammalian life span. Science 326:140-144.   DOI   ScienceOn
25 Tandon, P., C. A. Gallo, S. Khatri, J. F. Barger, H. Yepiskoposyan, and D. R. Plas. 2011. Requirement for ribosomal protein S6 kinase 1 to mediate glycolysis and apoptosis resistance induced by Pten deficiency. Proc. Natl. Acad.Sci. 108:2361-2365.   DOI   ScienceOn
26 Wang, Z., T. Liu, Y. Chen, X. Zhang, M. Liu, H. Fu, and D. Liu. 2012. Inhibition of mammalian target of rapamycin signaling by CCI-779 (Temsirolimus) induces growth inhibition and cell cycle arrest in Cashmere goat fetal fibroblasts (Capra hircus). DNA Cell Biol. 31:1095-1099.   DOI   ScienceOn
27 Valovka, T., F. Verdier, R. Cramer, A. Zhyvoloup, T. Fenton, H. Rebholz, M. L. Wang, M. Gzhegotsky, A. Lutsyk, G. Matsuka, V. Filonenko, L. Wang, C. G. Proud, P. J. Parker, and I. T. Gout. 2003. Protein kinase C phosphorylates ribosomal protein S6 kinase betaII and regulates its subcellular localization. Mol. Cell. Biol. 23:852-863.   DOI   ScienceOn
28 Wang, P., Y. F. Guan, H. Du, Q. W. Zhai, D. F. Su, and C. Y. Miao. 2012. Induction of autophagy contributes to the neuroprotection of nicotinamide phosphoribosyltransferase in cerebral ischemia. Autophagy 8:77-87.   DOI
29 Wang, Y., Q. Ding, C. J. Yen, W. Xia, J. G. Izzo, J. Y. Lang, C. W. Li, J. L. Hsu, S. A. Miller, and X. Wang. 2012. The crosstalk of mTOR/S6K1 and hedgehog pathways. Cancer cell 21:374-387.   DOI   ScienceOn
30 Wang Z., T. Fukazawa, T. Nishikawa, N. Watanabe, K. Sakurama, T. Motoki, S. Hatakeyama, O. Omori, T. Ohara, S. Tanabe, Y. Fujiwara, M. Takaoka, Y. Shirakawa, T. Yamatsuji, N. Tanaka, and Y. Naomoto. 2010. RAD001 offers a therapeutic intervention through inhibition of mTOR as a potential strategy for esophageal cancer. Oncol.Rep. 23:1167-1172.
31 Wullschleger, S., R. Loewith, and M. N. Hall. 2006. TOR signaling in growth and metabolism. Cell 124:471-484.   DOI   ScienceOn
32 Xu, X., K. Chen, S. Kobayashi, D. Timm, and D. Liang. 2012. Resveratrol attenuates doxorubicin-induced cardiomyocyte death via inhibition of p70 S6 kinase 1-mediated autophagy. J. Pharmacol. Exp. Ther. 341:183-195.   DOI
33 Zoncu, R., A. Efeyan, and D. M. Sabatini. 2011. mTOR: from growth signal integration to cancer, diabetes and ageing. Nat. Rev. Mol. Cell Biol. 12:21-35.   DOI   ScienceOn
34 Casas-Terradellas, E., I. Tato, R. Bartrons, F. Ventura, and J. L. Rosa. 2008. ERK and p38 pathways regulate amino acid signalling. Biochim.Biophys.Acta 1783:2241-2254.   DOI   ScienceOn
35 Arnold, K., L. Bordoli, J. Kopp, and T. Schwede. 2006. The SWISS-MODEL workspace: a web-based environment for protein structure homology modelling. Bioinformatics 22:195-201.   DOI   ScienceOn
36 Brian, M., E. Bilgen, and C. F. Diane. 2012. Regulation and function of ribosomal protein S6 kinase (S6K) within mTOR signalling networks. Biochem. J. 441:1-21.   DOI   ScienceOn
37 Carnevalli, L. S., K. Masuda, F. Frigerio, O. Le Bacquer, S. H. Um, V. Gandin, I. Topisirovic, N. Sonenberg, G. Thomas, and S. C. Kozma. 2010. S6K1 plays a critical role in early adipocyte differentiation. Dev. Cell 18:763-774.   DOI   ScienceOn
38 Castaneda, T. R., W. Abplanalp, S. H. Um, P. T. Pfluger, B. Schrott, K. Brown, E. Grant, L. Carnevalli, S. C. Benoit, and D. A. Morgan. 2012. Metabolic control by S6 kinases depends on dietary lipids. PloS One 7:e32631. doi: 10.1371/journal.pone.0032631   DOI
39 Charron, J., V. Bissonauth, and V. Nadeau. 2012. Implication of MEK1 and MEK2 in the establishment of the blood-placenta barrier during placentogenesis in mouse. Reprod. BioMed. Online 25:58-67.   DOI   ScienceOn
40 Guex, N., and M. C. Peitsch. 1997. SWISS(c)\MODEL and the Swiss(c)\Pdb Viewer: an environment for comparative protein modeling. Electrophoresis 18:2714-2723.   DOI   ScienceOn
41 Hamilton, D. L., A. Philp, M. MacKenzie, and K. Baar. 2010. Prolonged activation of S6K1 does not suppress IRS or PI-3 kinase signaling during muscle cell differentiation. BMC Cell Biol. 11:37. doi: 10.1186/1471-2121-11-37.   DOI   ScienceOn
42 Hannan, K. M., Y. Brandenburger, A. Jenkins, K. Sharkey, A. Cavanaugh, L. Rothblum, T. Moss, G. Poortinga, G. A. McArthur, and R. B. Pearson. 2003. mTOR-Dependent regulation of ribosomal gene transcription requires S6K1 and is mediated by phosphorylation of the carboxy-terminal activation domain of the nucleolar transcription factor UBF. Mol. Cell. Biol. 23:8862-8877.   DOI   ScienceOn
43 Hannan, K. M., G. Thomas, and R. B. Pearson. 2003. Activation of S6K1 (p70 ribosomal protein S6 kinase 1) requires an initial calcium-dependent priming event involving formation of a high-molecular-mass signalling complex. Biochem. J. 370:469-477.   DOI   ScienceOn
44 Hay, N., and N. Sonenberg. 2004. Upstream and downstream of mTOR. Genes Dev. 18:1926-1945.   DOI   ScienceOn
45 Julien, L. A., A. Carriere, J. Moreau, and P. P. Roux. 2010. mTORC1-activated S6K1 phosphorylates Rictor on threonine 1135 and regulates mTORC2 signaling. Mol. Cell. Biol. 30:908-921.   DOI   ScienceOn
46 Karapetsas, A., A. Giannakakis, M. Pavlaki, M. Panayiotidis, R. Sandaltzopoulos, and A. Galanis. 2011. Biochemical and molecular analysis of the interaction between ERK2 MAP kinase and hypoxia inducible factor-1alpha. Int. J. Biochem. Cell. Biol. 43:1582-1590.   DOI   ScienceOn
47 Kim, D., A. Akcakanat, G. Singh, C. Sharma, and F. Meric-Bernstam. 2009. Regulation and localization of ribosomal protein S6 kinase 1 isoforms. Growth Factors 27:12-21.   DOI   ScienceOn
48 Laplante, M., and D. M. Sabatini. 2012. mTOR signaling in growth control and disease. Cell 149:274-293.   DOI   ScienceOn