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Pathogenic Vibrio spp. Isolated from the Gwangan Beach of Busan in 2003

  • Park Mi-Yeon;Park Chan-Woong;Kwon Chil-Sung;Chang Dong-Suck
    • Fisheries and Aquatic Sciences
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    • v.7 no.1
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    • pp.10-15
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    • 2004
  • A total of 52 pathogenic Vibrio strains was isolated from the Gwangan Beach during summer in 2003. The isolated vibrios were composed of 6 different species: V. parahaemolyticus, V. cholerae non O1, V. fluvialis, V. vulnificus, V. alginolyticus, and V. mimicus. V. parahaemolyticus was most predominant as $46\%$ (24/52), V. cholerae non O1 was the second with $23\%$ (12/52), and V. fluvialis was the third with $17\%$ (9/52). Among the isolated strains, 22 strains showed hemolytic, proteolytic or ureolytic activity. Eight strains showed both hemolysin and protease activities, and either 6 strains showed only hemolysin activities and 7 strains only protease activities. Only one strain of V. parahaemolyticus isolates showed urease activity. The urease-positive V. parahaemolyticus strain (V. parahaemolyticus S25) showed the same biochemical characteristics as the reference strain, V. parahaemolyticus KCTC 2471 (urease­negative) except for urease production. To compare the degree of virulence of Vibrio strains having different pathogenic factors, hemolysin, protease, or urease-positive strains were injected into groups of 10 each of ICR mice (7- to l0-week-old male). The lethal rate of urease-positive V. parahaemolyticus S25 was significantly high, being $70\%$. Protease-positive strains showed $40-60\%$ of lethal rate. Hemolysin-positive strains showed no mortality, similar to non-pathogenic V. parahaemolyticus KCTC 2471 and V. parahaemolyticus FM12.

High-Cell-Density Fed-Batch Culture of Saccharomyces cerevisiae KV-25 Using Molasses and Corn Steep Liquor

  • Vu, Van Hanh;Kim, Keun
    • Journal of Microbiology and Biotechnology
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    • v.19 no.12
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    • pp.1603-1611
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    • 2009
  • High-cell-density cultivation of yeast was investigated using the agricultural waste products corn steep liquor (CSL) and molasses. The Saccharomyces cerevisiae KV-25 cell mass was significantly dependent on the ratio between C and N sources. The concentrations of molasses and CSL in the culture medium were statistically optimized at 10.25% (v/v) and 16.87% (v/v), respectively, by response surface methodology (RSM). Batch culture in a 5-l stirred tank reactor using the optimized medium resulted in a cell mass production of 36.5 g/l. In the fed-batch culture, the feed phase was preceded by a batch phase using the optimized medium, and a very high dried-cell-mass yield of 187.63 g/l was successfully attained by feeding a mixture of 20% (v/v) molasses and 80% (v/v) CSL at a rate of 22 ml/h. In this system, the production of cell mass depended mainly on the agitation speed, the composition of the feed medium, and the glucose level in the medium, but only slightly on the aeration rate.

Analysis on the Positional Accuracy of the Non-orthogonal Two-pair kV Imaging Systems for Real-time Tumor Tracking Using XCAT (XCAT를 이용한 실시간 종양 위치 추적을 위한 비직교 스테레오 엑스선 영상시스템에서의 위치 추정 정확도 분석에 관한 연구)

  • Jeong, Hanseong;Kim, Youngju;Oh, Ohsung;Lee, Seho;Jeon, Hosang;Lee, Seung Wook
    • Progress in Medical Physics
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    • v.26 no.3
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    • pp.143-152
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    • 2015
  • In this study, we aim to design the architecture of the kV imaging system for tumor tracking in the dual-head gantry system and analyze its accuracy by simulations. We established mathematical formulas and algorithms to track the tumor position with the two-pair kV imaging systems when they are in the non-orthogonal positions. The algorithms have been designed in the homogeneous coordinate framework and the position of the source and the detector coordinates are used to estimate the tumor position. 4D XCAT (4D extended cardiac-torso) software was used in the simulation to identify the influence of the angle between the two-pair kV imaging systems and the resolution of the detectors to the accuracy in the position estimation. A metal marker fiducial has been inserted in a numerical human phantom of XCAT and the kV projections were acquired at various angles and resolutions using CT projection software of the XCAT. As a result, a positional accuracy of less than about 1mm was achieved when the resolution of the detector is higher than 1.5 mm/pixel and the angle between the kV imaging systems is approximately between $90^{\circ}$ and $50^{\circ}$. When the resolution is lower than 1.5 mm/pixel, the positional errors were higher than 1mm and the error fluctuation by the angles was greater. The resolution of the detector was critical in the positional accuracy for the tumor tracking and determines the range for the acceptable angle range between the kV imaging systems. Also, we found that the positional accuracy analysis method using XCAT developed in this study is highly useful and will be a invaluable tool for further refined design of the kV imaging systems for tumor tracking systems.

Fabrication of New Co-Silicided Si Field Emitter Array with Long Term Stability (Co-실리사이드를 이용한 새로운 고내구성 실리콘 전계방출소자의 제작)

  • Chang, Gee-Keun;Kim, Min-Young;Jeong, Jin-Cheol
    • Korean Journal of Materials Research
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    • v.10 no.4
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    • pp.301-304
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    • 2000
  • A new triode type Co-silicided Si FEA(field emitter array) was realized by Co-silicidation of Co coated Si FEA and its field emission properties were investigated. The field emission properties of the fabricated device through the unit pixel with $45{\times}45$ tip array in the area of $250{\mu\textrm{m}}{\times}250{\mu\textrm{m}}$ under high vacuum condition of $10^{-8}Torr$ were as follows : the turn-on voltage was about 35V and the anode current was about $1.2\mu\textrm{A}(0.6㎁/tip)$ at the bias of $V_A=500V\;and\; V_G=55V$. The fabricated device showed the stable electrical characteristics without degradation of field emission current for the long term operation except for the initial transient state. The low turn-on voltage and the high current stability of the Co-silicided Si FEA were due to the thermal and chemical stability and the low work function of silicide layer formed at the surface of Si tip.

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LOCAL AND GLOBAL EXISTENCE AND BLOW-UP OF SOLUTIONS TO A POLYTROPIC FILTRATION SYSTEM WITH NONLINEAR MEMORY AND NONLINEAR BOUNDARY CONDITIONS

  • Wang, Jian;Su, Meng-Long;Fang, Zhong-Bo
    • Bulletin of the Korean Mathematical Society
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    • v.50 no.1
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    • pp.37-56
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    • 2013
  • This paper deals with the behavior of positive solutions to the following nonlocal polytropic filtration system $$\{u_t=(\mid(u^{m_1})_x{\mid}^{{p_1}^{-1}}(u^{m_1})_x)_x+u^{l_{11}}{{\int_0}^a}v^{l_{12}}({\xi},t)d{\xi},\;(x,t)\;in\;[0,a]{\times}(0,T),\\{v_t=(\mid(v^{m_2})_x{\mid}^{{p_2}^{-1}}(v^{m_2})_x)_x+v^{l_{22}}{{\int_0}^a}u^{l_{21}}({\xi},t)d{\xi},\;(x,t)\;in\;[0,a]{\times}(0,T)}$$ with nonlinear boundary conditions $u_x{\mid}{_{x=0}}=0$, $u_x{\mid}{_{x=a}}=u^{q_{11}}u^{q_{12}}{\mid}{_{x=a}}$, $v_x{\mid}{_{x=0}}=0$, $v_x|{_{x=a}}=u^{q21}v^{q22}|{_{x=a}}$ and the initial data ($u_0$, $v_0$), where $m_1$, $m_2{\geq}1$, $p_1$, $p_2$ > 1, $l_{11}$, $l_{12}$, $l_{21}$, $l_{22}$, $q_{11}$, $q_{12}$, $q_{21}$, $q_{22}$ > 0. Under appropriate hypotheses, the authors establish local theory of the solutions by a regularization method and prove that the solution either exists globally or blows up in finite time by using a comparison principle.

A Integrated Circuit Design of DC-DC Converter for Flat Panel Display (플랫 판넬표시장치용 DC-DC 컨버터 집적회로의 설계)

  • Lee, Jun-Sung
    • Journal of the Institute of Electronics and Information Engineers
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    • v.50 no.10
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    • pp.231-238
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    • 2013
  • This paper describes a DC-DC converter IC for Flat Panel Displays. In case of operate LCD devices various type of DC supply voltage is needed. This device can convert DC voltage from 6~14[V] single supply to -5[V], 15[V], 23[V], and 3.3[V] DC supplies. In order to meet current and voltage specification considered different type of DC-DC converter circuits. In this work a negative charge pump DC-DC converter(-5V), a positive charge pump DC-DC converter(15V), a switching Type Boost DC-DC converter(23V) and a buck DC-DC converter(3.3V). And a oscillator, a thermal shut down circuit, level shift circuits, a bandgap reference circuits are designed. This device has been designed in a 0.35[${\mu}m$] triple-well, double poly, double metal 30[V] CMOS process. The designed circuit is simulated and this one chip product could be applicable for flat panel displays.

A Study of on Minimizing the Number of V\ulcorner/V\ulcorner Pins in Simultaneous Switching Environment (동시 스위칭 환경에서 V\ulcorner/V\ulcorner Pin 수의 최소화를 위한 연구)

  • Bae, Yun-Jeong;Lee, Yun-Ok;Kim, Jae-Ha;Kim, Byeong-Gi
    • The Transactions of the Korea Information Processing Society
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    • v.7 no.7
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    • pp.2179-2187
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    • 2000
  • This paper provides a heuristic analysis technique which determines an optimal number of V\ulcorner/V\ulcorner pads meeting allowable Simultaneous Switching Noise(SN) budget, early in the design phase. Until now, in determining the number of V\ulcorner/V\ulcorner pads, designers had to simulate packaging models case by case in the design phase or roughly allocate the power/ground pins in an inaccurate way according to typical design rules. However, due to the high density and frequency trends of IC technologies, the V\ulcorner/V\ulcorner pads allocation method can affect an adverse effect on IC operations, which requires more accurate and efficient methods be devised. Thus, this paper proposes an analytic V\ulcorner/V\ulcorner pads calculation method that gives a practical help for packaging designs early in the design phase. The proposed method is applied to a design example of a 1/8x208 pin plastic quad flat package (PQFP) and the results are verified through simulation using HSPICE.

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Implementation of Electronic Nose System applicable to MPEG-V(ISO/IEC 23005) Standardization (MPEG-V(ISO/IEC 23005) 표준적용이 가능한 전자코 시스템 구현)

  • Lim, Hea-Jin;Choi, Jang-Sik;Jeon, Jin-Young;Byun, Hyung-Gi
    • Journal of Sensor Science and Technology
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    • v.25 no.6
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    • pp.388-393
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    • 2016
  • MPEG-V(ISO/IEC 23005) standardizes normative sensory effects metadata and sensory devices command for adapting the sensory effects between the virtual world and the real world. MPEG-V(Virtual) standardization has been carried out by 3DG(Dimensional Graphics) ad-hoc group inside MPEG Working Group(ISO IEC JTC1/SC29/WG11). For the scent effect, one of the sensory effects within MPEG-V, we proposed an olfactory interaction model including electronic nose and scent display to the ad-hoc group. Recently, we proposed types and elements related to the electronic nose as a sensor defined in MPEG-V standard for olfactory interaction. At the 114th MPEG meeting, the types and elements were consequently reflected on MPEG-V CD(Committee Draft) 4th edition. In this paper, we implement an electronic nose system applicable to MPEG-V standard by using MPEG-V schema, encoder, and decoder in order to assess their adequacy.

Characteristics of Redox Flow Battery Using the Soluble Lead Electrolyte (납이 용해된 전해액을 사용하는 레독스 플로우 전지의 특성)

  • An, Sang-Yong;Kim, Eung-Jin
    • Journal of the Korean Electrochemical Society
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    • v.14 no.4
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    • pp.214-218
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    • 2011
  • The electrochemical characteristics and performance of redox flow battery using the soluble lead has been evaluated. Cyclic voltammetry was performed on the materials to evaluate deposition and dissolution of lead and lead dioxide. In the negative region, a reduction peak is not observed, and on the reverse scan, on-set voltage is observed at -0.47 V(vs SCE). In the positive region, the distinct peak is observed on the forward and reverse scan. The charge/discharge experiments were carried out graphite electrode in the beaker cell. The charging(deposition) of lead occurs at around 0.5 V(vs SCE) and discharging(dissolution) of lead occur at around 0.25 V(vs SCE). The potential difference is about 0.25 V. The charging(deposition) of dioxide lead is at 1.77 V(vs SCE) and discharging(dissolution) is at around 0.95 V(vs SCE) during first cycle. On subsequent cycles, the charging of dioxide lead starts at below 1.5 V(vs SCE), after a period the voltage increase to 1.7 V(vs SCE). The voltage of discharging is stable at around 1.0 V(vs SCE).

The Effects of Dietary Biotite V Supplementation on Growth Performance, Nutrients Digestibility and Fecal Noxious Gas Content in Finishing Pigs

  • Chen, Y.J.;Kwon, O.S.;Min, B.J.;Shon, K.S.;Cho, J.H.;Kim, I.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.8
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    • pp.1147-1152
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    • 2005
  • Two experiments were conducted to evaluate the effects of dietary Biotite V (BV) supplementation on growth performance, nutrients digestibility and fecal noxious gas content in finishing pigs. In Exp. 1, a total of eighty pigs (initial body weight 88.0${\pm}$1.35 kg) were used in a 35-d growth trial. Pigs were blocked by weight and allotted to five dietary treatments in a randomized complete block design. There were four pigs per pen and four pens per treatment. Dietary treatments included: 1) Control (CON; basal diet), 2) 200 mesh BV1.0 (basal diet+200 mesh Biotite V 1.0%), 3) 325 mesh BV1.0 (basal diet+325 mesh Biotite V 1.0%), 4) 200 mesh BV2.0 (basal diet+200 mesh Biotite V 2.0%) and 5) 325 mesh BV2.0 (basal diet+325 mesh Biotite V 2.0%). Through the entire experimental period, there were no significant differences in ADG, ADFI and gain/feed among the treatments (p>0.05). With the addition of Biotite V in diet, DM and N digestibilities were increased significantly (p<0.01). Also, Ca and P digestibilities tended to increase in pigs fed Biotite V supplemented diet (p<0.01) compared to pigs fed control diet. Supplementation of Biotite V in diet reduced the fecal $NH_3-N$ and volatile fatty acid (VFA) compared to CON treatment (p<0.01). In Exp. 2, a total of sixty four pigs (initial body weight 84.0${\pm}$1.05 kg) were used in a 35-d growth trial. Pigs were blocked by weight and allotted to four dietary treatments in a randomized complete block design. There were four pigs per pen and four pens per treatment. Dietary treatments included: 1) LP (low protein diet), 2) HP (high protein diet), 3) LP+BV (low protein diet+325 mesh Biotite V 1.0%) and 4) HP+BV (high protein diet+325 mesh Biotite V 1.0%). Through the entire experimental period, ADG and gain/feed tended to increase in HP and HP+BV treatments, however, there were no significant differences (p>0.05) among the treatments. With the addition of Biotite V in diets, digestibilities of nutrients (DM, N, Ca and P) were increased significantly (p<0.01). The addition of Biotite V in diets reduced the ammonia emissions in feces (p<0.01). Supplementation of Biotite V in diets also reduced the fecal propionic acid, butyric acid and acetic acid (p<0.01) compared to pigs fed diets without Biotite V. In conclusion, supplementation of Biotite V can increase nutrients digestibility and reduce fecal $NH_3-N$ and volatile fatty acid (VFA) concentrations in finishing pigs.