Il Kyu Cho;Gyeong Hwan Lee;Woo Young Cho;Yun-Su Jeong;Danbi Kim;Kil Yong Kim;Gi-Woo Hyoung;Chul Hong Kim
Korean Journal of Environmental Agriculture
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v.41
no.4
/
pp.355-364
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2022
BACKGROUND: Pre-harvest interval and decline pattern of thiamethoxam were determined in kiwifruit using liquid chromatography-tandem mass spectrometry (LCMS/MS). The study was carried out to propose import tolerance using OECD maximum residue limit (MRL) calculator for the export promotion of kiwifruit to Taiwan. METHODS AND RESULTS: The thiamethoxam residue in kiwifruit was determined by using the LC-TriQ-MS/MS with the analytical process to set up the import tolerance under greenhouse conditions for Taiwan. Excellent linearity was observed for all of the analytes with a determination coefficient (R2)≥0.99. The limit of quantification was determined to be 0.01 mg/kg for both thiamethoxam and clothianidin in kiwifruit. Linearity was determined from the co-efficient of determinants (R2) obtained from the seven-point calibration curve. The standard calibration curve showed as follows; 1) Site 1 (Gimje): y = 944,406X + 1,583 (R2=0.9995), 2) Site 2 (Goheung): y = 1,356,205X + 934 (R2=0.9983), and 3) Site 3 (Jangheung): y = 1,239,937X - 3,090 (R2=0.9908). The residue of thiamethoxam in the kiwifruit for three decline trials showed the range of 0.35 to 0.56 mg/kg in site 1 (Gimje), 0.24 to 0.55 mg/kg in site 2 (Goheung), and 0.28 to 0.42 mg/kg in site 3 (Jangheung), respectively. However, clothianidin was not detected in all of the treatments. The maximum residual amounts (decline) in the samples, sprayed according to the safe-use standard for thiamethoxam 10% WG in kiwifruit (30 days before harvest, 3 sprays every 7 days) were 0.56 mg/kg in site 1, 0.55 mg/kg in site 2, and 0.42 mg/kg in site 3, respectively. CONCLUSION(S): The import tolerance (IT) of thiamethoxam for kiwifruit may be proposed to be 0.9 mg/kg by using the OECD MRL calculator.
Journal of the Korea institute for structural maintenance and inspection
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v.27
no.2
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pp.33-41
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2023
The application of seismic separation joints that can improve the deformation capacity of piping is an effective way to improve seismic performance. Seismic separation joints capable of axial expansion and bending deformation are installed where deformation is expected and used for the purpose of safely protecting the piping. Bellows are flexible and have low stiffness, so they can be used as seismic separation joints because they have excellent ability to respond to relatively large deformation. In this study, the seismic performance and limit state for bending deformation of 2-ply and 3-ply bellows specimens were evaluated. Seismic performance was evaluated by applying an increasing cyclic load to consider low-cycle fatigue due to seismic load. In order to confirm the margin for the limit state of the evaluated seismic performance, an experiment was conducted in which a cyclic loading of constant amplitude was applied. As a result of the experiment, it was confirmed that the bellows specimen was made of stainless steel and had a high elongation, so that the 2-ply bellows specimen had the limit performance of resisting within 3 cycles even at the maximum forced displacement of the 3-ply bellows specimen.
Journal of Korean Society of Disaster and Security
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v.16
no.2
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pp.1-13
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2023
As interest in urban safety has increased since COVID-19, various institutions have developed and used indicators that evaluate the safety city model. Yongsan-gu was ranked No. 1 in 2021 by Social Safety Index evaluation and was selected as the safest city in Korea. However, the Itaewon disaster in Yongsan-gu in 2022 caused many casualties. The study of indicators for evaluating cities' safety was necessary. This study aims to examine domestic and foreign safe city models and review the differences between each model and the indicators used to evaluate safe cities. As a result of collecting 11 safe city models and analyzing each evaluation index, safe city models can be classified into program-based safe city models, such as the World Health Organization's International safe community and the UN Office for Disaster Risk Reduction's International Safe city. Considering the diversification of threats to safety, it is reasonable to comprehensively consider digital security, health safety, infrastructure safety, personal safety, environmental safety, traffic safety, fire safety, crime safety, life safety, suicide, and infectious diseases when evaluating safe cities as evaluation parameters.
Jin-Soo Kim;Jae-Bong Park;Kwang-Rim Park;Dong-Uk Park;Sung-Wan Kim
Journal of the Korea institute for structural maintenance and inspection
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v.27
no.5
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pp.120-129
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2023
Hanger cable tension is a major response that can determine the integrity and safety of suspension bridges. In general, the vibration method is used to estimate hanger cable tension on operational suspension bridges. It measures natural frequencies from hanger cables and indirectly estimates tension using the geometry conditions of the hanger cables. This study estimated the hanger cable tension of the Palyeong Bridge using a vision-based system. The vision-based system used digital camcorders and tripods considering the convenience and economic efficiency of measurement. Measuring the natural frequencies for high-order modes required for the vibration method is difficult because the hanger cable response measured using the vision-based system is displacement-based. Therefore, this study proposed a back analysis technique for estimating tension using the natural frequencies of low-order modes. Optimization for the back analysis technique was performed by defining the difference between the natural frequencies of hanger cables measured in the field and those calculated using finite element analysis as the objective function. The direct search method that does not require the partial derivatives of the objective function was applied as the optimization method. The reliability and accuracy of the back analysis technique were verified by comparing the tension calculated using the method with that estimated using the vibration method. Tension was accurately estimated using the natural frequencies of low-order modes by applying the back analysis technique.
Sepsis is a systemic inflammatory response, with manifestations in multiple organs by pathogenic infection. Currently, there are no promising therapeutic strategies. Signal transducer and activator of transcription 3 (STAT3) is a cell signaling transcription factor. Niclosamide is an anti-helminthic drug approved by the Food and Drug Administration (FDA) as a potential STAT3 inhibitor. C57BL/6 mice were treated with an intraperitoneal injection of lipopolysaccharide (LPS). Niclosamide was administered orally 2 hours after the LPS injection. This study found that Niclosamide improved the survival and lung injury of LPS-induced mice. Niclosamide decreased the levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and interleukin-1β (IL-1β), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lactate dehydrogenase (LDH) in serum. The effects of Niclosamide on phosphoinositide 3-kinase (PI3K), AKT, nuclear factor-κB (NF-κB), and STAT3 signaling pathways were determined in the lung tissue by immunoblot analysis. Niclosamide reduced phosphorylation of PI3K, AKT, NF-κB, and STAT3 significantly. Furthermore, it reduced the phosphorylation of STAT3 by LPS stimulation in RAW 264.7 macrophages. Niclosamide also reduced the LPS-stimulated expression of proinflammatory mediators, including IL-6, TNF-α, and IL-1β. Niclosamide provides a new therapeutic strategy for murine sepsis models by suppressing the inflammatory response through STAT3 inhibition.
The fire risk and fire safety of four types of wood were comprehensively evaluated according to Chung's equation-XII. White ash, willow, fraxinus mandshurica, and sagent cherry trees were selected as test specimens. A cone calorimetery (ISO 5660-1) was used to examine the combustion characteristics of the test piece, and finally, the fire risk rating (FRR) was predicted using the fire risk index-XII (FRI-XII). The predicted fire performance index-X (FPI-X) and fire growth index-X (FGI-X) ranged from 469.03 to 1109.73 s2/kW and 0.0009 to 0.0280 kW/s2, respectively. Additionally, the fire performance index-XI (FPI-XI) and fire growth index-XI (FGI-XI) ranged from 0.41 to 0.97 and 1.11 to 3.11, respectively. The fire risk index-XII (FRI-XII), representing a fire risk rating, showed that the fire risk of frasxinus mandsurica tree (FM) was very high at 7.60 (fire risk rating: D). And it was compared with Chung's equation-IX, fire risk index-IX (FRI-IX). The fire risk ratings according to FRI-IX and FRI-XII were generally high for willow and frasxinus mandsurica trees. Additionally, the results of FRI-XII and FRI-IX had a similar relationship, and the size of each fire safety rating closely matched each other.
Jihun Kim;Bonhyuk Goo;Hyongjun Kim;Kyoungsuk Seo;Myungjin Oh;Myungseok Ryu;Sang-Hoon Yoon;Kwang Ho Lee;Hyun-Jong Lee;Jungtae Leem;Hyungsun Jun;Jeong Ihn Sook;Sung Woon Choi;Tae Wook Lee;Yeonhak Kim;Yoona Oh;Kunhyung Kim;Gi Young Yang;Eunseok Kim
The Journal of Korean Medicine
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v.45
no.1
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pp.182-201
/
2024
Objectives: This study was conducted to develop a standard acupotomy consent form that takes into account the unique characteristics of Korean Medicine. The study was motivated by the increasing importance of patient autonomy and the growing number of legal disputes related to medical malpractice in the clinical field of Korean Medicine. Methods: The analysis phase of the study involved a survey of the current status of acupotomy consent forms in Korean Medicine hospitals nationwide. The items of each form were analyzed based on the contents of the Medical law and the standard contract for medical procedures of the Fair Trade Commission (FTC). In the development and evaluation phase, the items and contents of the acupotomy consent form were evaluated using a 5-point Likert scale and content validity was assessed through two rounds of Delphi surveys. In the improvement phase, the contents of the consent form were revised based on the results of a survey of inpatient and outpatient patients in the Department of Acupuncture and Moxibustion at Pusan National University Korean Medicine Hospital, and real-time online meeting. The final version of the standard acupotomy consent form was completed after undergoing proofreading and corrections by a linguistics expert. Results: Only 30% of Korean Medicine hospitals have implemented acupotomy consent forms. The items of the consent forms did not fully include the items presented in the Medical act and the standard contract for medical procedures of the FTC. To address this issue, two rounds of Delphi surveys and a real-time discussion were conducted with a panel of 12 experts on 27 preliminary items of consent forms. The items and contents that met the criteria for content validity ratio, convergence, and consensus were derived. Based on the derived items and content, a standard acupotomy consent form was developed. Conclusions: The standard consent form for acupotomy is anticipated to ensure patient autonomy and enhance transparency and liability in acupotomy. Furthermore, it is expected to serve as evidence in case of medical disputes related to acupotomy and contribute as a reference document for the development of standard consents forms for various procedures of Korean Medicine. However, the limitations of the study include that the survey of consent forms was limited to only training hospitals of Korean Medicine, and the standard consent form is only applicable to adults in Korea. Future studies are needed to address these limitations.
Journal of the Korean Institute of Landscape Architecture
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v.40
no.4
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pp.36-50
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2012
This study was carried out to find contradiction factors on laws for children's activity spaces and facilities and to suggest the rational options to control and manage those spaces and facilities by environmental and landscape planning methods. The results of this study are as follows: 1. The major laws related to the environmental safety for children's activity spaces are "Environmental Health Act (ERA)" for managing the environmental safety of children's activity spaces; "Safety Supervision Law of Children's Play Facilities(SSLCPF)" for the inspection and management for safety of children's play facilities; "Quality Management and Industrial Products Safety Management Law(QMIPSML)" for managing safety certification on children's play equipments. 2. The interior space such as "living room" by the Children's Welfare Law(CWL), "Children Park" by the Act on Urban Parks and Green Spaces(AUPGS), "classroom" on private educational institutes by the Act on Establishment and Operation Private Lesson and Training(AEOPLT) and "nursing room" of child care center smaller than $430m^2$ are needed to be managed as an activity space. 3. In order to reduce industrial burden in the production, establishment, construction, and operation and to minimize unwilling extra burden in the administration effort due to legally double regulate, it is necessary to mitigate the inspections on the equipment certificate from QMIPSML and overlapped or different factors and standards must be unified. With this study, the landscape domain could he enlarged from producing, import of play equipment and establishment, construction and operation of play facilities for a comprehensive range of activity spaces, and the landscape industry such as engineering industry, academic research, management, etc.
Open-pit mines require constant monitoring as they can cause surface changes and environmental disturbances. In open-pit mines, there is little vegetation at the mining site and can be monitored using InSAR (Interferometric Synthetic Aperture Radar) coherence imageries. In this study, activities occurring in mine were analyzed by applying the recently developed InSAR coherence-based NDAI (Normalized Difference Activity Index). The March 5 Youth Mine is a North Korean mine whose development has been expanded since 2008. NDAI analysis was performed with InSAR coherence imageries obtained using Sentinel-1 SAR images taken at 12-day intervals in the March 5 Youth Mine. First, the area where the elevation decreased by about 75.24 m and increased by about 9.85 m over the 14 years from 2000 was defined as the mining site and the tailings piles. Then, the NDAI images were used for time series analysis at various time intervals. Over the entire period (2017-2019), average mining activity was relatively active at the center of the mining area. In order to find out more detailed changes in the surface activity of the mine, the time interval was reduced and the activity was observed over a 1-year period. In 2017, we analyzed changes in mining operations before and after artificial earthquakes based on seismic data and NDAI images. After the large-scale blasting that occurred on 30 April 2017, activity was detected west of the mining area. It is estimated that the size of the mining area was enlarged by two blasts on 30 September 2017. The time-averaged NDAI images used to perform detailed time-series analysis were generated over a period of 1 year and 4 months, and then composited into RGB images. Annual analysis of activity confirmed an active region in the northeast of the mining area in 2018 and found the characteristic activity of the expansion of tailings piles in 2019. Time series analysis using NDAI was able to detect random surface changes in open-pit mines that are difficult to identify with optical images. Especially in areas where in situ data is not available, remote sensing can effectively perform mining activity analysis.
Purpose : The measurement of radiation survival using a clonogenic assay, the established standard, can be difficult and time consuming. In this study, We have used the MTT assay, based on the reduction of a tetrazolium salt to a purple formazan precipitate by living cells, as a substitution for clonogenic assay and have examined the optimal condition for performing this assay in determination of radiation sensitivity. Materials and Methods : Four human cancer cell lines - PCI-1, SNU-1066, NCI-H630 and RKO cells have been used. For each cell line, a clonogenic assay and a MTT assay using Premix WST-1 solution, which is one of the tetrazolium salts and does not require washing or solubilization of the precipitate were carried out after irradiation of 0, 2, 4, 6, 8, 10 Gy. For clonogenic assay, cells in $25\;cm^2$ flasks were irradiated after overnight incubation and the resultant colonies containing more than 50 cells were scored after culturing the cells for $10\~14$ days. For MTT assay, the relationship between absorbance and cell number, optimal seeding cell number, and optimal timing of assay was determined. Then, MTT assay was performed when the irradiated cells had regained exponential growth or when the non-irradiated cells had undergone four or more doubling times. Results : There was minimal variation in the values gained from these two methods with the standard deviation generally less than $5\%$, and there were no statistically significant differences between two methods according to t-test in low radiation dose (below 6 Gy). The regression analyses showed high linear correlation with the $R^2$ value of $0.975\~0.992$ between data from the two different methods. The optimal cell numbers for MTT assay were found to be dependent on plating efficiency of used cell line. Less than 300 cells/well were appropriate for cells with high plating efficiency (more than $30\%$). For cells with low plating efficiency (less than $30\%$), 500 cells/well or more were appropriate for assay. The optimal time for MTT assay was after 6 doubling times for the results compatible with those of clonogenic assay, at least after 4 doubling times was required for valid results. In consideration of practical limits of assay (12 days, in this study) cells with doubling time more than 3 days were inappropriate for application. Conclusion : In conclusion, it is found that MTT assay can successfully replace clonogenic assay of tested cancer cell lines after irradiation only if MTT assay was undertaken with optimal assay conditions that included plating efficiency of each cell line and doubling time at least.
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