• 한방안이비인후피부과학회지
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• 제20권2호통권33호
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• pp.199-212
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• 2007

Objective : The purpose of this clinical research was to investigate the effects of Seunggaltang on patients with atopic dermatitis. Methods : Thirteen patients were treated with Seunggaltang and ten patients were treated with placebo for 8 weeks. We observed skin humidity, skin sebum,, transepidermal water loss, skin melanin, skin erythema, total IgE class and number of allergen. And Clinic Index of Atopic Dermatitis(Extent, Intensity, Subjective symptoms, Total score) was used to evaluate the effects of Seunggaltang. Statistical analysis was performed by using paired sample T-test. Statistical significance was achieved if the probability was less than 5%(P<0.05). The result were as follows : 1. Statistically, Seunggaltang didn't showed significant effect on skin humidity, skin sebum, skin transepidermal water loss, skin melanin and skin erythema. 2. Statistically, Seunggaltang showed significant effect on Clinic Index of atopic dermatitis. 3. Statistically, Seunggaltang didn't showed significant effect on total IgE class and number of allergen. 4. Before and after treatment, the results of blood test and urinalysis were normal. Conclusion : We speculate that Seunggaltang has some therapeutic effects in mitigating the symptoms of atopic dermatitis.

• 한방안이비인후피부과학회지
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• 제23권3호
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• pp.154-164
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• 2010

Objective : The purpose of this clinical research was to investigate the effects of herbal cosmetics containing Phellinus linteus extracts on patients with atopic dermatitis. Methods : A total of 35 patients who visited Daegu Hanny Oriental Medical Center from November 4th, 2008 to December 28th, 2008 were included in this study. In this study, we observed transepidermal water loss, skin moisture content, modified scorad index of atopic dermatitis and pruritic degree. Statistical analysis was performed by using paired t-test and wilcoxon signed ranks test. Statistical significance was achieved if the probability was less than 5%(p<0.05). Results : Statistically, herbal cosmetics containing Phellinus linteus extracts showed siginificant effect on transepidermal water loss, skin moisture content, modified scorad index of atopic dermatitis and pruritic degree(p<0.05). And satisfaction after using herbal cosmetics was near good. Conclusions : Considering the above results, we have concluded that herbal cosmetics containing Phellinus linteus extracts have the remarkable effects on atopic dermatitis.

• 대한한의학회지
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• 제28권4호
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• pp.18-26
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• 2007

Objective : To study the effect of the Insamyangyoung-tang(ISYT) extract on the skin barrier function, the skin pH, skin humidity and transepidermal water loss(TEWL) were measured and histological changes were observed in DNCB(2,4-dinitrochloro-benzen)-induced contact dermatitis(CD) hairless mice. Methods : The male hairless mice were divided into three groups. Each group consisted of 15 mice. The normal group which had acetone- olive oil applied. The control group which had intentionally induced CD by DNCB and it was fed normal saline orally. The ISYT group which had intentionally induced CD by DNCB and it was fed ISYT extract orally for 7 days. The three groups were checked 24h, 48h and 72h later after inducing CD, and the skin pH, skin humidity and TEWL were observed. Tissue samples were taken, and damage to the epithelial cell was observed. Statistical analysis was performed by using one way-ANOVA: significance was set at p values less than 5% (p<0.05). Results : ISYTextract efficiently maintained the pH balance, it kept the skin humidity at a normal level, and it inhibited TEWL of the DNCB-induced CD hairless mouse. The damage to the epithelium was decreased and the regeneration power of the skin was increased in the ISYT group. Conclusion : Insamyangyoung-tang has a good effect on the skin barrier function of DNCB induced contact dermatitis hairless mice.

• Journal of Ginseng Research
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• 제44권1호
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• pp.8-13
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• 2020

Atopic dermatitis (AD) is a chronic and relapsing inflammatory disease that affects 1%-20% of people worldwide. Despite affecting many people, AD current treatments, such as corticosteroids and calcineurin inhibitors, have not only harmful secondary effects but are also often ineffective. Therefore, natural nontoxic compounds are on high demand for developing new effective AD treatments. Panax ginseng Meyer has been used traditionally for its promising healing and restorative properties to treat many diseases including skin disorders, reason why in this review we want to explore the research performed with AD and P. ginseng as well as determining its potential for new drug development. Previous researches have shown that P. ginseng has positive effects in AD patients such as lower eczema area and severity index, transepidermal water loss, and immunoglobulin E levels and better quality of sleep. In vivo animal models, as well, have shown positive results to P. ginseng and derived ginsenosides, such as the decrease of transepidermal water loss, immunoglobulin E levels in serum, allergy-related cytokines, and downregulation of NF-κB, MAPK, and Ikaros pathways. All of these previous data suggest that P. ginseng and its derived ginsenosides are undoubtedly a nontoxic effective option to treat AD.

• 한국식품위생안전성학회지
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• 제36권1호
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• pp.77-85
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• 2021

본 연구는 PFEG(글루코실세라마이드 4.0% 함유)가 저 미네랄 특수사료(HR-AD)를 섭취시킨 헤어리스 마우스에서 발생하는 피부장벽 붕괴에 대한 억제작용이 있는지를 확인하고자 하였다. 4주간의 투여를 통해 정상군(Rabo MR Stock)과 비교하여 대조군(HR-AD)에서는 경피 수분 손실량(TEWL)이 서서히 상승하여 피부장벽기능의 붕괴가 발생함과 동시에 표피 수분량 및 각층 수분량도 서서히 저하되는 것이 확인되었다. 육안적으로는 깊은 주름 형성이 확인되었으며 조직학적 소견에서는 표피의 비후와 각화 항진이 확인되었다. 그러나 PFEG를 섭취시킨 시험군에서는 대조군에서 유발되는 피부장벽기능 붕괴에 대한 억제 효과가 확인되었다. 즉, 글루코실세라마이드 0.01% 배합군 및 0.1% 배합군에서는 섭취 시작 2주일째 이후 야기되는 TEWL 상승을 완전히 억제하였다. 또한, 저하되는 표피 수분량, 각층 수분량에 대해서도 유의하게 억제하여 피부 보습 기능을 유지하고 있었다. 육안적으로도 깊은 주름 형성은 억제되었으며, 조직학적인 검토에서도 표피의 비후나 각화 항진은 확인되지 않았다. 이러한 결과는 HR-1 마우스의 피부 세라마이드 생산 개선에 기인한 결과라고 예상된다. 즉, HR-AD 섭취에 의해 HR-1 마우스는 세라마이드를 포함한 피부 지질의 함량 및 구성에 이상이 생기고, 피부 수분량이 급격히 줄어들어 피부 보습 능력이 저하된다. 그런데 PFEG의 혼합 투여는 손상된 피부의 세라마이드 함량 및 구성을 정상과 가깝게 만들어, 대조군에서 나타나는 증상의 발현을 억제하는 것으로 판단된다. 이상의 결과로부터 PFEG의 섭취는 피부장벽기능의 손상으로 발생하는 다양한 피부 수분 손실을 억제함으로써 피부 보습 개선에 우수한 효능이 있음을 알 수 있었다. 따라서 PFEG가 피부 보습에 도움을 주는 건강기능성 원료로서 이용 가능성이 높음을 확인할 수 있다.

• Journal of Applied Biological Chemistry
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• 제60권2호
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• pp.155-159
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• 2017

이 연구의 목적은 5종의 식물추출물을 함유하는 보습력이 우수한 보습크림을 개발하여 피부개선 효능을 평가하는 것으로, 비단풀, 황백, 홍화, 치자, 오배자의 복합추출물을 함유하는 보습크림과 이를 포함하지 않는 보습크림 간의 임상연구를 수행하였다. 21명의 피험자에 대해 효과분석이 이루어졌으며, 각각의 분석은 3회 반복 하여 검증하였다. 식물유래 복합추출물이 함유된 보습크림(A)과 함유되지 않은 크림(B)과의 수분함량 비교분석에서 A 크림이 B 크림에 비해 50% 이상 높은 수준의 보습력을 지니고 있으며, 피부 경피수분손실량 및 홍반반응 분석에서도 A 크림이 B 크림에 비해 유의성 있는 개선효과를 나타내었다. 결론적으로 식물복합추출물이 함유된 크림은 인체 피부자극 유도 후의 피부자극 완화 효과 평가를 통해 피부자극 완화 및 개선에 도움을 줄 수 있을 것으로 판단되고, 관련 산업분야의 다양한 응용성을 가질 것으로 판단된다.

• 한방안이비인후피부과학회지
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• 제22권1호
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• pp.76-88
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• 2009

Back ground and Objective : There is a need for objectification and scientific verification of Pattern identification in Oriental medicine. The purpose of this study was to investigate the skin characteristics of Qi deficiency and Blood deficiency animal models. Material and Methods : Sprague-Dawley rats were divided into three groups: normal group, Qi deficiency group and Blood deficiency group. The Qi deficiency animal model was induced through restriction of food (75g/kg/day) for 20 days. Blood deficiency animal model was induced by bleeding from tail vein(0.3 ml/time) 8 times. The normal animal model was kept without any intervention. The general condition was observed by measuring body weight, body temperature, blood pressure, pulse rate, and hematological and biochemical parameters. The skin characteristics were observed by measuring the erythema index(EI), melanin index(Ml), transepidermal water loss(TEWL) and dermal microcirculation. Results : 1. In the Qi deficiency group, body weight was lower than the other groups. In the Qi deficiency group, blood pressure was lower than in the Normal group. There was no difference in body temperature and pulse rate between the three groups. 2. In the Qi deficiency group, blood sugar was lower than in the Blood deficiency group. There was no difference in triacylglycerol between the three groups. In the Qi deficiency group, the WBC count was lower than in the Blood deficiency group. RBC count was highest in the Qi deficiency group, Normal group and Blood deficiency group respectively. In the Qi deficiency group, Hb and Hct were higher than the other groups. 3. EI and MI were decreased in the Qi deficiency group, and EI showed a significant decrease. 4. EI and MI were increased in the Blood deficiency group, and MI showed a significant increase. 5. TEWL was significantly increased in the Qi deficiency group, while it was decreased in the Blood deficiency group, TEWL was highest in the Qi deficiency group, Normal group and Blood deficiency group respectively and all three groups showed significant difference. 6. In the Qi deficiency group, dermal microcirculation was lower than the other groups. Conclusion : The above results show that the erythema index decreases in the Qi deficiency model, and the melanin index increases in the Blood deficiency model. The Qi deficiency animal model shows an increase in transepidermal water loss, while the Blood deficiency animal model shows a decrease. Further studies should develop new models of Pattern Identification that are more specific.

• 한국응용과학기술학회지
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• 제34권4호
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• pp.727-736
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• 2017

본 연구에서는 임상시험의 대체시험법으로 시편을 이용한 화장품의 보습능 평가기법을 연구하였다. 4종의 시편(일본떡, 가래떡, 밀가루, 한천)에 대한 립 메이크업 제품의 발림성, 표면의 건조 특성, 수분손실율 등을 비교 평가하였다. 또한, 10종의 립 메이크업 제품(립스틱 5종, 립밤 3종, 립글로스 2종)을 대상으로 시편을 사용한 수분손실율과 임상평가를 통한 경피수분손실량을 분석하고 이들의 상관 관계를 조사하였다. 시편의 수분손실율은 한천 > 가래떡 > 일본떡 > 밀가루 순으로 한천시편의 수분 손실율(수분증발 민감도)이 가장 우수하였다. 또한, 시편소재 중 한천시편이 발림성, 표면 균열, 원료 수급 등의 측면에서 가장 적합하였다. 한천시편은 볼록하고 매끄러운 표면의 시편 제조가 가능한 열 전달이 낮은 플라스틱재질의 용기가 적합하였다. 한천시편을 이용한 립메이크업 제품군에 대한 수분손실율(보습능)과 임상시험법(경피수분손실)과 강한 상관관계를 보였다. 이러한 결과들은 본 연구에서 제안한 한천 시편을 이용한 보습능 평가 기법이 유용한 대체시험법 중 하나로 활용 가능함을 의미한다.

• 한방안이비인후피부과학회지
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• 제21권2호
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• pp.126-141
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• 2008

Objective : This clinical research was conducted to test patients with Atopic Dermatitis by external application with Yeongyuseungmatang in cosmetics. Methods : We gave scores to 33 patients who visited the Dept. of Oriental Medical Opthalmology & Otolaryngology & Dermatology of Semyung Jecheon Oriental Medical Hospital from April 17th, 2008 to June 12th. Seventeen patients were treated with Yeongyuseungmatang(Experimental group) and sixteen patients were treated with Atopico skin care cream(Control group) for 4weeks. We observed change of total IgE, eosinophil count, Skin Temperature, Transepidermal Water Loss(TEWL), Skin Hydration and Skin pH. Also Clinic Index of Atopic Dermatitis(SCORAD Index) and global assesment of efficacy were used to evaluate the effects of Yeongyuseungmatang. Statistical analysis was performed by using frequency analysis and descriptive analysis. Statistical significance was achieved if the probability was less than 5%(p<0.05). Results : 1. After 4 weeks of external application treatment, SCORAD Index in Yeongyuseungmatang was significant statistically decreased compared with control group. 2. After 4 weeks of external application treatment, eosinophil count in Yeongyuseungmatang was significant statistically decreased compared with control group but total IgE was very increased both Yeongyuseungmatang and control group. 3. Statistically, Yeongyuseungmatang and control group didn't showed significant effect on Skin Temperature was measured by Thermometer SM815. 4. Transepidermal Water Loss(TEWL) in Yeongyuseungmatang was significant statistically decreased compared with control group measured by Tewameter TM300. 5. Skin Hydration in Yeongyuseungmatang was significant statistically increased compared with control group measured by Corneometer CM825. 6. Statistically, Yeongyuseungmatang and control group didn't showed significant effect on Skin pH was measured by Skin-pH-Meter PH905. 7. After 4 weeks of external application treatment, Yeongyuseungmatang showed significant effect on global assesment of efficacy. Conclusion : Considering the above results, we can speculate that cosmetics with Yeongyuseungmatang has some therapeutical effects in mitigating the symptoms of atopic dermatitis.

• Preventive Nutrition and Food Science
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• 제17권4호
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• pp.245-253
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• 2012

Collagen tripeptide (CTP) is a functional food material with several biological effects such as improving dry skin and wound and bone fracture healing. This study focused on the anti-photoaging effects of CTP on a hairless mouse model. To evaluate the effects of CTP on UVB-induced skin wrinkle formation in vivo, the hairless mice were exposed to UVB radiation with oral administration of CTP for 14 weeks. Compared with the untreated UVB control group, mice treated with CTP showed significantly reduced wrinkle formation, skin thickening, and transepidermal water loss (TEWL). Skin hydration and hydroxyproline were increased in the CTP-treated group. Moreover, oral administration of CTP prevented UVB-induced MMP-3 and -13 activities as well as MMP-2 and -9 expressions. Oral administration of CTP increased skin elasticity and decreased abnormal elastic fiber formation. Erythema was also decreased in the CTP-treated group. Taken together, these results strongly suggest that CTP has potential as an anti-photoaging agent.