Browse > Article
http://dx.doi.org/10.13103/JFHS.2021.36.1.77

Effect of Oral Administration of Pineapple Fruit Extract Containing Glucosylceramide on Skin Barrier Function Improvement in Animal Model of Atopic Dermatitis  

Miyake, Yasuo (Research Center, Maruzen Pharmaceuticals Co., Ltd.)
Jo, Ho Young (Research and Development Center, Maruon Co., Ltd.)
Kim, Young-Dong (Research and Development Center, Maruon Co., Ltd.)
Yeom, Myeong-Hun (Research and Development Center, Maruon Co., Ltd.)
Publication Information
Journal of Food Hygiene and Safety / v.36, no.1, 2021 , pp. 77-85 More about this Journal
Abstract
Glucosylceramides (GluCer) are known to play an important role in both water retention and epidermal permeability barrier function in the mammalian stratum corneum. In this study, we investigated the effects of pineapple fruit extract containing glucosylceramides (PFEG) on the maintenance and recovery of skin barrier function using atopic dermatitis-induced animal models. Five-week-old male Hos:HR-1 mice were divided into four groups fed on standard diet, unsaturated fatty acids-deficient (HR-AD) diet, and HR-AD diet supplemented with 0.01% or 0.1% pineapple-GluCer. Skin barrier function was evaluated by transepidermal water loss (TEWL), dermal moisture content, moisture content of the stratum corneum and wrinkle formation. The control group (HR-AD administration group) showed increased transepidermal water loss (TEWL), while the epidermal moisture content and the moisture content of the stratum corneum slowly decreased. However, in the PFEG groups (with 0.1% or 0.01% glucosylceramide), the TEWL levels were significantly reduced at 2 weeks. The PFEG also helped maintain skin moisturizing function by significantly suppressing the decrease of the epidermal moisture content and the moisture content of the stratum corneum. These results show that the PFEG is effective for maintaining and improving the function of the skin barrier. Therefore, this study suggests that PFEG is a potential candidate material for skin functional foods.
Keywords
Pineapple fruit extract containing gulcosylceramide (PFEG); Glucosylceramide; Hairless mice; Skin barrier; Transepidermal water loss (TEWL);
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Imokawa, G., Abe, A., Jin, K., Higaki, Y., Kwashima, M., Hidano, A., Decreased level of ceramides in stratum corneum of atopic dermatitis: An etiologic factor in atopic dry skin?. J. Invest. Dermatol., 96, 523-526 (1991).   DOI
2 Candi, E., Schmidt, R., Melino, G., The cornified envelope: A model of cell death in the skin. Nat. Rev. Mol. Cell Biol., 6, 328-340 (2005).   DOI
3 Shimada, E., Aida, K., Sugawara, T., Hirata, T., Inhibitory effect of topical maize glucosylceramide on skin photoaging in UVA-irradiated hairless mice. J. Oleo Sci., 60, 321-325 (2011).   DOI
4 Inamine, M., Suzui, M., Morioka, T., Kinjo, T., Kaneshiro, T., Sugishita, T., Okada, T., Yoshimi, N., Inhibitory effect of dietary monoglucosylceramide 1-O-beta-glucosyl-N-2'-hydroxyarachidoyl-4,8-sphingadienine on two different categories of colon preneoplastic lesions induced by 1,2-dimethylhydrazine in F344 rats. Cancer Sci., 96, 876-881 (2005).   DOI
5 Takatori, R., Vu, P.L., Iwamoto, T., Satsu, H., Totsuka, M., Chida, K., Shimizu, M., Effects of oral administration of glucosylceramide on gene expression changes in hairless mouse skin: comparison of whole skin, epidermis, and dermis. Biosci. Biotechnol. Biochem., 77, 1882-1887 (2013).   DOI
6 Doopedia, (2021, January 21). Pineapple. Retrieved from https://www.doopedia.co.kr/doopedia/master/master.do?_method=view&MAS_IDX=101013000865575
7 Kwon, S.B., Lee, G.T., Choi, S.J., Lee, N.K., Park, H.W., Lee, K.S., Lee, K.K., Ahn, K.J., An, I.S., The effect of glycerin, hyaluronic acid and silicone oil on the hydration, moisturization and transepidermal water loss in human skin. Kor. J. Aesthet. Cosmetol., 11, 761-768 (2013).
8 Chang, M.Y., Kim, J.J., Lee, C.K., Moisturizers in cosmetics-classification of moisturizers by action mechanism-. J. Skin Barrier Res., 9, 18-26 (2007).
9 Rudolph, R., Kownatzki, E., Corneometric, sebumetric and TEWL measurements following the cleaning of atopic skin with a urea emulsion versus a detergent cleanser. Contact Dermatitis, 50, 354-358 (2004).   DOI
10 Kim, H.J., Jeong, S.K., New in trends skin barrier research. J. Skin Barrier Res., 14, 15-28 (2012).
11 Kim, D.S., Analyses of ethnic variations and physico-chemical factors that affect skin barrier functions. Master's thesis, Dong-Kuk University, Seoul, Korea (2010).
12 Nakano, F., Iwasaki, D., Nojima, J., Ohto, N., Kuwahara, H., Glucosylceramides from the fruits of pineapple. Japanese J. Pharm., 69, 66-67 (2015).
13 Uchiyama, T., Nakano, Y., Ueda, O., Mori, H., Nakashima, M., Noda, A., Ishizaki, C., Mizoguchi, M., Oral intake of glucosylceramide improves relatively higher level of transepidermal water loss in mice and healthy human subjects. J. Health Sci., 54, 559-566 (2008).   DOI
14 Toya, K., Kawashima, Y., Ohto, N., Nojima, J., Ikeoka, S., Tanabe, M., Kiso, A., Beautifying efficacies of pineapple fruit extract containing glucosylceramides. J. Soc. Cosmet. Chem. Jpn., 50, 306-313 (2017).   DOI
15 Ideta, R., Sakuta, T., Nakano, Y., Uchiyama, T., Orally administered glucosylceramide improves the skin barrier function by upregulating genes associated with the tight junction and cornified envelope formation. Biosci. Biotechnol. Biochem., 75, 1516-1523 (2011).   DOI
16 Kuwata, T., Hashimoto, T., Ohto, N., Kuwahara, H., Lee, J.W., Bamba, T., Mizuno, M., A metabolite of dietary glucosylceramide from pineapples improves the skin barrier function in hairless mice. J. Funct. Foods, 30, 228-236 (2017).   DOI
17 Oka, S., Ohto, N., Kuwahara, H., Mizuno, M., Oral administration of pineapple glucosylceramide improves defective epidermal barrier function by restoring diminished level of TGF-β in the skin. Eur. Food Res. Technol., 246, 867-874 (2020).   DOI
18 Ministry of Food and Drug Safety, 2020, Standards and specifications for health functional foods (No. 2020-92). Cheongju, pp. 132.
19 Jin, S.P., Lee, D.H., Epidermal structure and skin barrier. J. Skin Barrier Res., 16, 5-12 (2014).
20 Seo, Y.J., Epidermal structure and differentiation. J. Skin Barrier Res., 11, 3-12 (2009).
21 Kahraman, E., Kaykin, M., Bektay, H.S., Gungor, S., Recent advances on topical application of ceramides to restore barrier function of skin. Cosmetics, 6, 52 (2019).   DOI
22 Kwon, M.S., Choi, T.B., Kim, G.Y., The effect on the skin barrier function of ceramide. Kor. J. Aesthet. Cosmetol., 3, 131-137 (2005).
23 Kim, T. S., Lee, S.P., Yang, W.S., Kang, M.H., Murai, H., Okada, T., Lee, J.H., Park, I.B., Park, H.J., Improvement of skin moisture capacity through dietary beauty supplement containing ceramides derived from rice. Korean J. Food Sci. Technol., 44, 434-440 (2012).   DOI
24 Nam, J.J., Effect of Chrysanthemum zawadskii and Mentha arvensis on skin barrier function via keratinocytes differentiation. Master's thesis, Dong-Kuk University, Seoul, Korea (2012).
25 Matoltsy, A.G., Downes, A.M., Sweeney, T.M., Studies of the epidermal water barrier. J. Invest. Dermatol., 50, 19-26 (1968).   DOI
26 Elias, P.M., Lipids and the epidermal permeability barrier. Arch. Dermatol. Res., 270, 95-117 (1981).   DOI