• Title/Summary/Keyword: Three-dimensional culture

Search Result 262, Processing Time 0.027 seconds

Pineal Photoreceptor and Ganglion Cells in River Lamprey, Lampetra japonica -Two Types of Pineal Ganglion Cell-

  • Tamotsu, Satoshi;Kinugawa, Yoshimi;Kawano, Emi;Watanabe, Mai;Samejima, Michikazu;Oishi, Tadashi
    • Journal of Photoscience
    • /
    • v.9 no.2
    • /
    • pp.21-24
    • /
    • 2002
  • Pineal organs of poikilotherm vertebrates transform the environmental light information into a humoral message and a neuronal activity. The former is melatonin, and the latter is modulation of the impulse in ganglion cells. The ganglion cells are physiologically classified into luminosity (achromatic) type and chromatic one, as the neural activity is modulated in two ways. We attempted to classify the pineal ganglion cells with morphological characteristics by means of the three- dimensional reconstruction method. In the pineal ganglion cells of river lamprey, there are two different features, oval and spherical. For comparison of their projection region in the brain, the tracing investigation was also carried out. The application of the neural tracer near mesencephalic tegmentum showed that only oval-shaped ganglion cells were labeled in the pineal organ. These results suggest that the oval-shaped ganglion cell is functionally different from the spherical one.

  • PDF

A Study of the Patternmaking Methods for Mass Customization of the Men's Jacket (남성복 재킷의 Mass Customization을 위한 패턴 제작 방법 연구)

  • Oh, Seol-Young;Chun, Jong-Suk;Suh, Dong-Ae
    • The Research Journal of the Costume Culture
    • /
    • v.14 no.1
    • /
    • pp.40-47
    • /
    • 2006
  • Three-dimensional body scanners were used for years in the clothing manufacturing fields. The 3D body surface provide essential data to draft patterns for mass customization, virtual fit model, and computerized patternmaking systems. This research proposed the methods of drafting patterns for men's jacket by using three dimensional body scan data. Eight male subjects were scanned, the surface data was flattened. The differentials of the flattened body surface and the jacket draft were measured, and analyzed the regressions. To verify the fit of the patterns, the jacket was constructed by the regression formulae and tested by experts. The fit of the jacket were significantly improved rather than a ready-made suit especially the shoulder areas. This means that the methods that we proposed were good to improve the fit of the garments and could be used effectively to implement mass customization strategies in the apparel retail industry.

  • PDF

A Research of Madeleine Vionnet's Work II (Madeleine Vionnet의 작품에 관한 연구II -연구대상 작품의 제작되어진 패턴을 중심으로-)

  • 박선경
    • The Research Journal of the Costume Culture
    • /
    • v.4 no.1
    • /
    • pp.93-109
    • /
    • 1996
  • Madeleine Vionnet, a representative designer of the 1920′s, who made prefectly organized clothes with unique formativeness was the first to express fabric in a modern method and was called "The architect of fashion". This is because she developed a three dimensional design by expressing cloth-she rejected corset, should pad, etc-into elegant curved lines that do not stick to the body. By granting meaning to the beauty of the body and its movement in her own unique ways, she emphasized the formativeness in her work and gave shape to creative artistry. Expecially with the "Bias-Cut" she could express the lines of the body more flexibly and could make geometrical styles like the diamond shape dress or the triangle dress more effectively. Using the "Tired Bias cut" and "Handkerchief point" she let the skirt hemline dangle irregularly in geometrical forms, thus showing modern formative sense which forms a three dimensional solidness along the movement of the human body. Thus far, analyzed how the contemporary trend of art was reflected in her designs by studying her work; also investigated through her artistic characteristics and pattern method. Also be tried to find out what can be learned through her artistic view and superb formativeness as a designer.

  • PDF

Recent advances in organoid culture for insulin production and diabetes therapy: methods and challenges

  • Dayem, Ahmed Abdal;Lee, Soo Bin;Kim, Kyeongseok;Lim, Kyung Min;Jeon, Tak-il;Cho, Ssang-Goo
    • BMB Reports
    • /
    • v.52 no.5
    • /
    • pp.295-303
    • /
    • 2019
  • Breakthroughs in stem cell technology have contributed to disease modeling and drug screening via organoid technology. Organoid are defined as three-dimensional cellular aggregations derived from adult tissues or stem cells. They recapitulate the intricate pattern and functionality of the original tissue. Insulin is secreted mainly by the pancreatic ${\beta}$ cells. Large-scale production of insulin-secreting ${\beta}$ cells is crucial for diabetes therapy. Here, we provide a brief overview of organoids and focus on recent advances in protocols for the generation of pancreatic islet organoids from pancreatic tissue or pluripotent stem cells for insulin secretion. The feasibility and limitations of organoid cultures derived from stem cells for insulin production will be described. As the pancreas and gut share the same embryological origin and produce insulin, we will also discuss the possible application of gut organoids for diabetes therapy. Better understanding of the challenges associated with the current protocols for organoid culture facilitates development of scalable organoid cultures for applications in biomedicine.

A Study on Formation of Concepts of Architectural Space based on the Optical Dimension (시각적 차원에 의한 건축 공간의 개념 형성에 관한 연구)

  • Byun, Dae-Joong
    • Korean Institute of Interior Design Journal
    • /
    • v.19 no.5
    • /
    • pp.56-66
    • /
    • 2010
  • This study proposes a thesis of architectural concepts and visual dimension systems, and the comparison between steps of spatial formation and dimensional alteration. The second chapter, to form the basis of this study, explains the dimensional alterations and changes of fundamental notion of space. In the third chapter, history of space, architectural formations, and changes of the viewpoint are analyzed as objects of study. The forth chapter presents the interrelation between dimensional alteration and the transition in fundamental notion of space, demonstrating that modern architecture has been born from these cultural movements. Lastly, the fifth chapter suggests possibilities on further studies and the following conclusions: First, architectural spaces have been changed, in accordance with the changes of culture, art and the tools that regulate architectural design. Proportional regulations by two-dimensional tools and depth through three-dimensional drawings are created. Second, architectural spaces gained depth by recognizing movement and time that have induced formations to change, creating various aesthetic backgrounds and attempts. Third, the aesthetic background and cosmologic spatial concept have led the visualization and changes of architectural experience. It created the design tools and shapes originated in dynamism and vitality. Forth, diversification of fundamental spatial concepts has become palimpsest and complex, and been divided into four dimensions; expressional two-dimensional space, perspective three-dimensional space, forth-dimensional space of time and experience, and imagery space formed by body movement. Fifth, architecture has been influenced by the elevated viewpoint that understands the whole world as a space. It has evolved from the two-dimensional proportion principle, change of depth and vanishing point to multidimensional space of movement and time. Sixth, changes of fundamental notion of space have arisen from changes of visual dimensions in times. In other words, space has been developed from two-dimensional space to multidimensional space by accepting visual dimension, grasping distance, direction, depth, height, velocity, movement, gravity, power and structure.

Glucosamine Hydrochloride and N-Acetylglucosamine Influence the Response of Bovine Chondrocytes to TGF-β3 and IGF in Monolayer and Three-Dimensional Tissue Culture

  • Pizzolatti, Andre Luiz A.;Gaudig, Florian;Seitz, Daniel;Roesler, Carlos R.M.;Salmoria, Gean Vitor
    • Tissue Engineering and Regenerative Medicine
    • /
    • v.15 no.6
    • /
    • pp.781-791
    • /
    • 2018
  • BACKGROUND: Glucosamine hydrochloride (GlcN HCl) has been shown to inhibit cell growth and matrix synthesis, but not with N-acetyl-glucosamine (GlcNAc) supplementation. This effect might be related to an inhibition of critical growth factors (GF), or to a different metabolization of the two glucosamine derivatives. The aim of the present study was to evaluate the synergy between GlcN HCl, GlcNAc, and GF on proliferation and cartilage matrix synthesis. METHOD: Bovine chondrocytes were cultivated in monolayers for 48 h and in three-dimensional (3D) chitosan scaffolds for 30 days in perfusion bioreactors. Serum-free (SF) medium was supplemented with either growth factors (GF) $TGF-{\beta}$ ($5ng\;mL^{-1}$) and IGF-I ($10ng\;mL^{-1}$), GlcN HCl or GlcNAc at 1mM each or both. Six groups were compared according to medium supplementation: (a) SF control; (b) SF + GlcN HCl; (c) SF + GlcNAc; (d) SF + GF; (e) SF + GF + GlcN HCl; and (f) SF + GF + GlcNAc. Cell proliferation, proteoglycan, collagen I (COL1), and collagen II (COL2) synthesis were evaluated. RESULTS: The two glucosamines showed opposite effects in monolayer culture: GlcN HCl significantly reduced proliferation and GlcNAc significantly augmented cellular metabolism. In the 30 days 3D culture, the GlcN HCl added to GF stimulated cell proliferation more than when compared to GF only, but the proteoglycan synthesis was smaller than GF. However, GlcNAc added to GF improved the cell proliferation and proteoglycan synthesis more than when compared to GF and GF/GlcN HCl. The synthesis of COL1 and COL2 was observed in all groups containing GF. CONCLUSION: GlcN HCl and GlcNAc increased cell growth and stimulated COL2 synthesis in long-time 3D culture. However, only GlcNAc added to GF improved proteoglycan synthesis.

A study on the cultural ideology of narrative in 3D C.G. Animation (3D C.G. 애니메이션에 반영된 문화적 이데올로기 - <슈렉>을 중심으로)

  • Koh, Eun-Young
    • Cartoon and Animation Studies
    • /
    • s.6
    • /
    • pp.7-22
    • /
    • 2002
  • Animation constitutes the core of the media industry, which in turn lies at the center of the cultural industry. It is considered one of the industries where South Korea has the competitive edge over other countries. With the pool of customers getting wider, the genre of animation has become more and more diverse, forming a great market for it. Aware of this trend, this study focused on animation as a part of the pop culture, and on providing corresponding various viewpoints for future cultural studies. This researcher measured the practicality and persuasiveness of this study through Shreck, a three-dimensional C.G. animation which is acclaimed for its success in dismantling the old grammar of animation movies that represent the anti-Disney ideas. This researcher felt it imperative to heed the unique language of Shreck, which contains discourses on various cultural ideologies such as paradoxical structure that pits entertainment that is shown through dismantling of the canon, feminism and antifeminism against each other. This study analyzed the entertaining element of the animation genre by means of the Semiotics of Keith Moxey, thereby attempting to establish a legitimate social status of the genre, whose artfulness has been depreciated in the art society. In chapter II, this researcher examines the chronological development of three-dimensional C.G. animation that has shown a rapid advancement. Chapter III defines the cultural ideology of Shreck by exploring basic theories and texts employed in analysis of art works. This study started with the assumption that defines, from the viewpoint of symbology, the animation text as an aggregate of discourses on entertainment, and competitive and paradoxical ideologies. Then, this researcher analyzed the text and the generation process of meanings in Shreck. Consequently, this study has come to the following conclusions: First, Shreck induces changes of concepts about the canon by means of distorting and reversing the existing animation movies, which seems to reflect in the contemporary tendency of seeking new interpretations of entertainment. Second, Shreck shows up the cognitive changes of our age as to feminism by competing feminism against antifeminism. Although Shreck serves as a venue of competition between the two opposing ideas, it stops short of brushing off women as outsiders in society. Rather, it represents the resistance to the male chauvinism existing in the structures of animation and culture. As shown in the text analysis, Shreck presents an advent of a new ideology critical of the previous animation films. In addition, it reflects in the struggle between the pro-feminism on the part of the viewers and the anti-feminism that lies in the social and culture structure. This study, however, is limited in its scope and selection of subject. First, although this researcher has stressed the importance of understanding the animation as part of the pop culture and conducting researches within the historic paradigm, this study fails to provide an in-depth insight in the impacts that the changes in the C.G. industry and the systematic conditions may have on the three-dimensional C.G. animation genre. Furthermore, this study runs the risk of being understood as pro-American due to its selection of Shreck as its research subject.

  • PDF

Design and Implementation of Electronic Culture Atlas Based on Google Earth (구글어스 기반의 전자문화지도 설계 및 구현)

  • Kang, Ji-Hoon;Moon, Sang-Ho
    • Journal of the Korea Institute of Information and Communication Engineering
    • /
    • v.18 no.2
    • /
    • pp.357-363
    • /
    • 2014
  • The development of information technology grows the society and allows the user to generate more information and the requirements. According to recent growing interest in digital convergence technologies, digital convergence can be utilized by a variety of methods as a new study. Electronic culture atlas would be digitized cultural information that represents a particular area on the map. In detail, spatial data like as point, line, and area can be used to represent the culture information. Using electronic culture atlas, also, it is possible to provide and utilize information which is associated with time, space(map) and subject(culture). Thus, electronic culture atlas could be used as a method for the humanities or area studies and spread research results by displaying on the culture atlas. In general, existing electronic culture atlas used bitmap image mostly as a base map. Using bitmap image, it is difficult to represent to provide three-dimensional information of specific area. Also, it happens problem such as breakage occurs during zoom-in operation. To solve this problems, we design and implement electronic culture atlas based on Google Earth which is three-dimensional map service.

Past, Present, and Future of Brain Organoid Technology

  • Koo, Bonsang;Choi, Baekgyu;Park, Hoewon;Yoon, Ki-Jun
    • Molecules and Cells
    • /
    • v.42 no.9
    • /
    • pp.617-627
    • /
    • 2019
  • Brain organoids are an exciting new technology with the potential to significantly change our understanding of the development and disorders of the human brain. With step-by-step differentiation protocols, three-dimensional neural tissues are self-organized from pluripotent stem cells, and recapitulate the major millstones of human brain development in vitro. Recent studies have shown that brain organoids can mimic the spatiotemporal dynamicity of neurogenesis, the formation of regional neural circuitry, and the integration of glial cells into a neural network. This suggests that brain organoids could serve as a representative model system to study the human brain. In this review, we will overview the development of brain organoid technology, its current progress and applications, and future prospects of this technology.

Development of Three-Dimensional Knit Models through Rib & Purl Structures (리브편 조직과 펄편 조직을 이용한 입체 니트 구조의 개발)

  • Choi, Won-Seok;Lee, Youn-Hee
    • The Research Journal of the Costume Culture
    • /
    • v.18 no.1
    • /
    • pp.109-117
    • /
    • 2010
  • Knit fabrics are created on diverse machines with diverse knit loops and conditions to make different patterns and fabric types. Dimensional modifications of knit fabrics can also be achieved by numerous methods such as different knit-loop structures, different types of yarns, or different finishing processes including heat setting, steaming, chemical treatment et cetera. This research develops and explores sophisticated three-dimensional knit fabrics by combining the several different knit stitches including rib and purl. This study focuses on 3D knit models created on modern electronic weft (flat V-bed) knitting machines which have capability of individual needle selection. Several samples of the 3D knitted fabrics are also examined in this research. This research furthermore suggests new types of knitted fashion garment made by using the interesting physical effects.